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  • Functional
  • Functional characterisation of these channels has revealed a diversity of properties in that they may show inward or outward rectification, their activity may be modulated in different directions by protein phosphorylation, and their sensitivity to changes in intracellular or extracellular pH varies. (ebi.ac.uk)
  • In conclusion, the PUFA-activated K2P 2.1 and K2P 6.1 are expressed in murine lung and functional K2P -like channels contribute to endothelium hyperpolarization and pulmonary artery relaxation. (forskningsdatabasen.dk)
  • cytoplasmic
  • Channel activity increased with the cytoplasmic free Ca2+ concentration ([Ca2+]i) with a Hill plot giving a half-saturating [Ca2+] (K0.5) of 1.35 μM and slope of ≅3. (edu.au)
  • blockers
  • Moreover, the action potential firing rates evoked by the depolarizing current and BK Ca channel blockers were remarkably decreased by application of resveratrol. (biomedcentral.com)
  • Myography on pulmonary arteries showed that DHA induced concentration-dependent instantaneous relaxations that were resistant to endothelial removal and inhibition of NO and prostacyclin synthesis and to a cocktail of blockers of calcium-activated K(+) channels but were abolished by high extracellular (30 mM) K(+) -concentration. (forskningsdatabasen.dk)
  • cardiac
  • In eukaryotic cells, K + channels are involved in neural signalling and generation of the cardiac rhythm, act as effectors in signal transduction pathways involving G protein-coupled receptors (GPCRs) and may have a role in target cell lysis by cytotoxic T-lymphocytes [ PMID: 1373731 ]. (ebi.ac.uk)
  • activity
  • Ionomycin (1-10 μM) increased BK channel activity in the cell-attached recording configuration. (edu.au)
  • Protein
  • When the signaling molecule cAMP docks onto the potassium channel, it causes a rearrangement and shift in the protein scaffold," explains Julia Kowal, first author of this study. (innovations-report.com)
  • Functional characterisation of these channels has revealed a diversity of properties in that they may show inward or outward rectification, their activity may be modulated in different directions by protein phosphorylation, and their sensitivity to changes in intracellular or extracellular pH varies. (ebi.ac.uk)
  • These results suggest that the cardiac K ATP channel protein possesses endogenous cytoprotective properties when transfected into a noncardiac cell type. (ahajournals.org)
  • Characterization, purification and affinity labeling of the brain [3H]glibenclamide binding protein, a putative neuronal ATP-regulated K+ channel. (springer.com)
  • The high resolution crystallographic structure of the rat Kvα1.2/β2 channel has recently been solved (Protein Databank Accession Number 2A79​), and then refined in a lipid membrane-like environment (PDB: 2r9r​). (wikipedia.org)
  • When moving through the selectivity filter of the potassium channel, the water-K+ interactions are replaced by interactions between K+ and carbonyl groups of the channel protein. (wikipedia.org)
  • Hence the potassium cations are well "solvated" by the protein carbonyl groups, but these same carbonyl groups are too far apart to adequately solvate the sodium cation. (wikipedia.org)
  • In eukaryotic cells, K+ channels are involved in neural signalling and generation of the cardiac rhythm, act as effectors in signal transduction pathways involving G protein-coupled receptors (GPCRs) and may have a role in target cell lysis by cytotoxic T-lymphocytes. (wikipedia.org)
  • The G protein-coupled inwardly-rectifying potassium channels (GIRKs) are a family of inward-rectifier potassium ion channels which are activated (opened) via a signal transduction cascade starting with ligand-stimulated G protein-coupled receptors (GPCRs). (wikipedia.org)
  • transmembrane
  • The simplicity of the two transmembrane helices in KcsA, as opposed to the six in many eukaryotic ion channels, also provided a method to understand the mechanisms of K+ channels conduction at a more rudimentary level, thereby providing even great impetus for the study of KcsA. (wikipedia.org)
  • The transmembrane cation channel superfamily was defined in InterPro and Pfam as the family of tetrameric ion channels. (wikipedia.org)
  • They are described as minimally having two transmembrane helices flanking a loop which determines the ion selectivity of the channel pore. (wikipedia.org)
  • gene
  • In Potassium Channels: Methods and Protocols , experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. (springer.com)
  • Cloning of a probable potassium channel gene from mouse brain. (ebi.ac.uk)
  • Such genetic methods allowed the genetic identification of the "Shaker" K+ channel gene in Drosophila before ion channel gene sequences were well known. (wikipedia.org)
  • intracellular
  • Intracellular ADP activates K+ channels that are inhibited by ATP in an insulin-secreting cell line. (springer.com)
  • This family of ion channels is, for the most part, activated by intracellular Ca2+ and contains 8 members in the human genome. (wikipedia.org)
  • However, some of these channels (the KCa4 and KCa5 channels) are responsive instead to other intracellular ligands, such as Na+, Cl−, and pH. (wikipedia.org)
  • BK channels are activated (opened) by changes in membrane electrical potential and/or by increases in concentration of intracellular calcium ion (Ca2+). (wikipedia.org)
  • A cytoplasmic C-terminal domain (CTD) consisting of a pair of RCK domains that assemble into an octameric gating ring on the intracellular side of the tetrameric channel. (wikipedia.org)
  • The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. (wikipedia.org)
  • inactivation
  • Continued investigation of the various opened and closed, inactive and active conformations of KcsA by other imaging methods such as ssNMR and EPR have since provided even more insight into channel structure and the forces gating the switch from channel inactivation to conduction. (wikipedia.org)
  • adenosine
  • Adenosine-5'triphosphate-sensitive ion channels in neonatal rat cultured central neurones. (springer.com)
  • The potassium channel RNA editing signal is an RNA element found in human Kv1.1 and its homologues which directs the efficient modification of an adenosine to inosine by an adenosine deaminase acting on RNA (ADAR). (wikipedia.org)
  • blockade
  • Conclusions These findings suggest that the mechanism of action of the rare proarrhythmic effects of the nonsedating antihistamines appears to be secondary to potassium channel blockade. (ahajournals.org)
  • Until recently, the prevailing theory was that either K ATP openers or ischemia resulted in the enhanced shortening of action potential duration (APD), which shortened phases 2 and 3 of the action potential and resulted in a blockade of L-type calcium channels and a reduction in calcium overload during ischemia and/or reperfusion. (ahajournals.org)
  • cardiac myocytes
  • To determine the cellular electrophysiological basis for the proarrhythmic effects of these drugs, this study was designed to investigate the actions of two H 1 receptor antagonists, terfenadine and astemizole, on various potassium (K + ) channels of cardiac myocytes. (ahajournals.org)
  • Cardiac myocytes expressing the native endogenous K ATP channel were also exposed to the same chemical hypoxia-reoxygenation protocol and demonstrated a marked increase in cellular calcium that was significantly attenuated by pinacidil, an effect that was abolished by glyburide. (ahajournals.org)
  • KATP channels were first identified in cardiac myocytes by the Akinori Noma group in Japan. (wikipedia.org)
  • molecules
  • Potassium channels are a key component of this electrical circuit and are controlled either by an electrical impulse or through signaling molecules. (innovations-report.com)
  • The change from one state to the other happens quickly and synchronously, due to C-terminus multimerization among proximate KATP channel molecules. (wikipedia.org)