• The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. (nih.gov)
  • Golden Promise carries a loss-of-function mutation in HvDep1, an AGG3-type subunit encoding gene that positively regulates culm elongation and seed size in barley. (lu.se)
  • Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. (lsbio.com)
  • Our results demonstrate that BR signaling positively regulates chilling tolerance via ABA biosynthesis in tomato. (sciencenet.cn)
  • The CFTR protein also regulates the function of other channels, such as those that transport positively charged particles called sodium ions across cell membranes. (medlineplus.gov)
  • Positively regulates HOXC8 and HOXC6 gene expression. (icr.ac.uk)
  • Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. (umn.edu)
  • CodY also positively regulates bacteria virulence. (health-ground.com)
  • This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. (affbiotech.com)
  • Here we show that the Calmodulin 1 (CaM1) gene, which encodes Ca2+-binding protein calmodulin 1, positively regulates leaf senescence in Arabidopsis. (ibs.re.kr)
  • RPK1 positively regulates the expression of the CaM1 gene, and the CaM1 protein, in turn, up-regulates RbohF gene expression. (ibs.re.kr)
  • Furthermore, we identified PU.1 as positive transcriptional regulator of ATG3, ATG4D, and ATG5. (hindawi.com)
  • Functions as a transcriptional regulator. (icr.ac.uk)
  • Rgg simply because a worldwide transcriptional regulator, promotes S. suis 2 bacterial success during pathogen-host connections12. (health-ground.com)
  • CodY is definitely a global transcriptional regulator of gene manifestation in low G? (health-ground.com)
  • Thus, our results demonstrate that changes in neuronal activity initiate competing signaling pathways that positively and negatively regulate the growth of the dendritic arbor. (jneurosci.org)
  • A total of 34 TFs were postulated as co-regulators in both pathways directly or indirectly, which might play important roles in carotenoids and flavonoids accumulation in CNO fruit. (researchsquare.com)
  • The prominence of Kv7 channels as regulators of electrical excitability may be ascribed to their unique properties, such as a very negative threshold of activation (negative to −50 mV), absence of time-dependent inactivation, and high sensitivity to signal transduction pathways that influence their probability of opening, either positively or negatively ( Delmas and Brown, 2005 ). (aspetjournals.org)
  • Additionally, we describe how neuropathology can negatively influence these pathways, and conclude by discussing opportunities to optimize the intramuscular administration route to maximize gene delivery and thus therapeutic potential. (frontiersin.org)
  • Genes and pathways associated with lactation number in cows were identified for the first time to date, and we found that many were comparable to those known to be associated with ageing in humans and model organisms. (biomedcentral.com)
  • In spite of the advantages and successful applications, the identification of causal oncogenic pathways and driver-regulators remains a challenge [ 6 ]. (biomedcentral.com)
  • To identify novel molecular targets within the ubiquitination pathway that positively regulate migration we conducted a loss-of-function genetic screen using an epithelial cell line derived from human triple-negative breast cancer (MDAMB231) infected with a pooled shRNA interference library. (nature.com)
  • Furthermore, 15/15 candidate TFs might regulate at least three key genes and contribute to carotenoids/flavonoids accumulation in CNO fruit. (researchsquare.com)
  • The Goal of research is to characterize the molecular mechanisms, including identifying genes, which regulate biological processes in crops that effect agronomical performance, postharvest quality and shelf life and yield of processed products made from fruit crops. (purdue.edu)
  • RNA-binding proteins have increasingly been identified as important regulators of gene expression given their ability to bind distinct RNA sequences and regulate their fate. (bvsalud.org)
  • We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. (umn.edu)
  • In addition, we identified a number of differentially expressed genes that are involved in epigenetics, chromatin remodeling, apoptosis, and metabolism that may point to underlying molecular mechanisms that regulate pluripotency and trophoblast differentiation in humans. (mpg.de)
  • In addition, mutual antagonism between two of these and other master regulators have been shown to regulate lineage determination. (lu.se)
  • TFs being expressed and the downstream differentiation target been established as candidates to regulate both pluripotency and genes being shut off [11]. (lu.se)
  • The binding of the positively charged aminoglycosides to the negatively charged biofilm matrix polymers of P. aeruginosa will delay the penetration of aminoglycosides, [ 13 ] while the penetration of fluoroquinolones occurs immediately and without delay. (medscape.com)
  • in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation. (sjtu.edu.cn)
  • In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
  • The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. (microbiologyresearch.org)
  • The effects of PTE on osteoclast differentiation and the expression of osteoclast-specific genes and proteins were determined. (researchgate.net)
  • Additionally, PTE efficaciously suppressed the expression of key osteoclast-specific genes and proteins. (researchgate.net)
  • Differentially expressed candidate genes for ageing previously identified in the human blood transcriptome up-regulated in PP cows were mainly associated with T-cell function ( CCR7 , CD27 , IL7R , CAMK4 , CD28 ), mitochondrial ribosomal proteins ( MRPS27 , MRPS9 , MRPS31 ), and DNA replication and repair ( WRN ). (biomedcentral.com)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • From our screen, we identified the Ubiquitin-specific protease 19 (USP19) as a candidate gene associated with the regulation of cell migration. (nature.com)
  • Binding of VENTX to the homeobox domain binding (HDB) site in the promoter of the macrophage colony stimulating factor receptor (M-CSFR, CSF1R) gene results in up-regulation of CSF1R transcription. (reactome.org)
  • Analyzing gene expression of PSGs during aging in the long-lived naked mole-rat and up-regulation in the short-lived rat, we found a pattern fitting the antagonistic pleiotropy theory of aging. (biorxiv.org)
  • Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor β1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. (umn.edu)
  • These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb. (umn.edu)
  • The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. (cancerindex.org)
  • The top three clusters from modules more highly expressed in the PP cows all involved regulation of gene transcription, particularly zinc fingers. (biomedcentral.com)
  • Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)
  • Moreover, genome instability was attributed to impaired autophagy and several autophagy genes with tumor suppressor functions (e.g. (hindawi.com)
  • In order to identify novel molecular targets of tumor cell migration and invasion we performed a genetic screen with an shRNA library against ubiquitination pathway-related genes. (nature.com)
  • This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. (genetex.com)
  • Srivastava A, Chung SH, Fatima T, Datsenka T, Handa AK, Mattoo AK (2007) Polyamines as anabolic growth regulators revealed by transcriptome analysis and metabolite profiles of tomato fruits engineered to accumulate spermidine and spermine. (purdue.edu)
  • Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. (molcells.org)
  • The protein encoded by this gene belongs to the GSK-3-binding protein family. (nih.gov)
  • This gene encodes a member of the regulator of G-protein signaling family. (nih.gov)
  • Variation in regulator of G-protein signaling 17 gene (RGS17) is associated with multiple substance dependence diagnoses. (nih.gov)
  • Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. (lsbio.com)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. (genetex.com)
  • As a positive regulator of plant immunity, E3 ligase SAUL1 is guarded by NLR protein SOC3. (ubc.ca)
  • The CFTR gene provides instructions for making a protein called the CF transmembrane conductance regulator (CFTR). (medlineplus.gov)
  • Most of these mutations change single protein building blocks (amino acids) in the CFTR protein or delete a small amount of DNA from the CFTR gene. (medlineplus.gov)
  • Ramakrishna W., Deng Z., Ding C.-K, Handa A. K., Ozminkowski Jr R. H. (2003) A novel small heat shock protein gene, vis1, contributes to pectin depolymerization and juice viscosity in tomato fruit. (purdue.edu)
  • Smooth muscle cells express Kv7.4 and Kv7.5 voltage-dependent potassium channels, which have each been implicated as regulators of smooth muscle contractility, though they display different sensitivities to signaling via cAMP/protein kinase A (PKA) and protein kinase C (PKC). (aspetjournals.org)
  • The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • The protein encoded by this gene is a member of the BCL-2 family. (affbiotech.com)
  • To coordinate expression of virulence genes during infection, S. aureus uses 2-component systems, transcription factors ( 5 ) and regulatory or small RNAs (sRNAs), which function as positive ( 6 ) or negative ( 7 ) virulence determinants. (cdc.gov)
  • In addition, K-2157 harbored two plasmids: one of 113,644 bp (KPC+) and another of 230,602 bp, containing virulence genes, in addition to an integrative and conjugative element (ICE) embedded on its chromosome, revealing that the presence of these mobile genetic elements mediates the convergence between virulence and antibiotic resistance. (bvsalud.org)
  • In strains resulted in the overexpression of several virulence genes. (health-ground.com)
  • It activates toxin gene manifestation by regulating the global regulator AtxA in or capsulation, but led to attenuated virulence of a wild-type strain inside a mouse model of illness25. (health-ground.com)
  • In general, this PIP5K1C study would enhance our understanding of virulence rules mediated by CodY in mutation inhibited cell growth of 2 To investigate virulence rules by in 2, we constructed a mutant strain for gene (Fig. 1a). (health-ground.com)
  • Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
  • Mutations in the CFTR gene disrupt the function of the chloride channel, preventing the usual flow of chloride ions and water into and out of cells. (medlineplus.gov)
  • More than 1,000 mutations in the CFTR gene have been identified in people with cystic fibrosis. (medlineplus.gov)
  • Disease-causing mutations in the CFTR gene alter the production, structure, or stability of the chloride channel. (medlineplus.gov)
  • A few mutations in the CFTR gene have been identified in people with isolated problems affecting the digestive or respiratory system. (medlineplus.gov)
  • Molecular docking studies suggest that cranberry-derived proanthocyanidin binds to QS transcriptional regulators, mainly interacting with their ligand binding sites. (nature.com)
  • Here, we determined that overexpression of the BR biosynthesis gene DWARF ( DWF ) or the key BR signaling gene BRASSINAZOLE \RESISTANT1 ( BZR1 ) increases ABA levels in response to chilling stress via positively regulating the expression of the ABA biosynthesis gene 9 \CIS \EPOXYCAROTENOID DIOXYGENASE1 ( NCED1 ). (sciencenet.cn)
  • Yellowing of leaves, accumulation of reactive oxygen species (ROS), and expression of the senescence-associated gene 12 (SAG12) were significantly enhanced in CaM1 overexpression plants. (ibs.re.kr)
  • Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)
  • It has been proposed that NnERF4/5 could have be important regulators of fresh-cut lotus root browning, and that the relationships of NnERF4/5 and NnPAL1 , NnPPOA , and NnPOD2/3 should to be studied further. (ashs.org)
  • Then, the regulatory TFs for the mesenchymal signature genes were inferred from the use of master regulator analysis (MRA) together with or without stepwise linear regression method (SLR). (biomedcentral.com)
  • Also, under/over-expression studies of the master regulator Oct4 have revealed that some self-renewal/pluripotency as well as differentiation genes are expressed in a biphasic manner with respect to the concentration of Oct4. (lu.se)
  • The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. (mpg.de)
  • To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. (mpg.de)
  • A model based which contains the Oct4/Sox2/Nanog core as well its interaction upon regulatory mechanisms inferred from ChIP-on-chip and with a few other key genes. (lu.se)
  • Cluster identity was assigned based on the top 10 marker genes of each cluster ( Table S2 ), followed by inspection of RNA in situ hybridization patterns. (stowers.org)
  • The selected TFs showed a comparable or slightly better prognostic performance than the original 85 signature genes in spite of greatly reduced number of marker genes from 85 to 10. (biomedcentral.com)
  • The cyclic GMP-AMP synthase (cGAS)-Stimulator of Interferon Genes (STING) pathway is a critical defender to sense various pathogens and trigger innate immunity of mammalian cells. (frontiersin.org)
  • Our results confirm the role of AGG3-type subunit-encoding genes in shaping plant architecture, but interestingly also indicate that the impact HvDep1 has on yield in barley is both genotypically and environmentally sensitive. (lu.se)
  • This may explain why widespread exploitation of variation in AGG3-type subunit-encoding genes has not occurred in temperate cereals while in rice the DEP1 locus is widely exploited to improve harvestable yield. (lu.se)
  • Strikingly, K-2157 displayed a resistome composed of ß-lactam resistance genes (blaSHV-190, blaTEM-1, blaOXA-9, and blaKPC-2), the fosfomycin resistance gene fosA, and the fluoroquinolones resistance genes oqxA and oqxB. (bvsalud.org)
  • Although QACs may play an important role in the propagation of antibiotic resistance gene (ARGs), the potential contribution and mechanism remains unclear. (bvsalud.org)
  • We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)
  • We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. (stowers.org)
  • Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. (sjtu.edu.cn)
  • 3 cows were pooled, and the gene lists subjected to David functional annotation cluster analysis. (biomedcentral.com)
  • Another cluster included genes encoding enzymes in the mitochondrial beta-oxidation pathway. (biomedcentral.com)
  • Blom A. M. , Webb J., Villoutreix B. and *Dahlbäck B. (1999) A cluster of positively charged amino acids in the N-terminal modules of C4BP a-chain is crucial for the C4b binding and factor I cofactor function. (lu.se)
  • Using the genome of SC19 as the template, the DNA fragment was amplified by PCR before becoming cloned into the pSET4s vector to produce pSET4s-was inserted into the endogenous gene to disrupt the practical manifestation of CodY (Fig. 1a). (health-ground.com)
  • These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
  • Cox regression analysis identifies independent prognostic factors for these patients, and enrichment analysis of prognosis-related genes was also performed. (biomedcentral.com)
  • We constructed a 13-gene prognostic model for LUAD patients. (biomedcentral.com)
  • Within this context, we constructed a prognostic model of ERS-related genes, combining the risk scores in the model with clinical characteristics (age, sex, staging, etc.) to establish a nomogram, and found its performance in estimating patient survival was excellent. (biomedcentral.com)
  • Gene expression signatures have been commonly used as diagnostic and prognostic markers for cancer subtyping. (biomedcentral.com)
  • In order to identify prognostic master regulators, we took the known 85 prognostic signature genes for colorectal cancer and inferred their upstream TFs. (biomedcentral.com)
  • Our network approach leads to the identification of the upstream transcription factors for prognostic signature genes to provide leads to their regulatory mechanisms. (biomedcentral.com)
  • The set of 319 PSGs contains regulators of mTOR and is enriched in functional terms associated with (i) processes that are regulated by the mTOR pathway, e.g. translation, autophagy and mitochondrial biogenesis, (ii) the immune system and (iii) antioxidant defense. (biorxiv.org)
  • Although individualized teaching strategies may change epigenetic gene expression and improve reading and writing during earlier stages of education, the underlying gene sequences may continue to play an etiological role for individuals with expressive writing disorder, especially as curriculum requirements increase in nature, complexity, and volume with increasing academic complexity. (medscape.com)
  • Although the genes that encode carotenogenesis and flavonoids enzymes are well characterized, the transcriptional regulatory mechanisms that are related to the pathway genes remain to be investigated. (researchsquare.com)
  • Finally, a hierarchical model for the regulatory network among the pathway genes and TFs was proposed. (researchsquare.com)
  • Their upstream regulators such as transcription factors (TFs) may take a more critical role as drivers or master regulators to provide better clues on the underlying regulatory mechanisms and therapeutic applications. (biomedcentral.com)
  • Notably, these TFs were selected solely from inferred regulatory links using gene expression profiles and included many TFs regulating tumorigenic processes such as proliferation, metastasis, and differentiation. (biomedcentral.com)
  • applied a reverse engineering method for context-specific transcriptional regulatory networks to 176 gene expression profiles from high-grade glioblastoma (HGG) patients. (biomedcentral.com)
  • The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
  • 2006). The PI3K/Akt signalling pathway is strongly implicated in breast cancer, and published gene array studies have suggested that PIPP mRNA expression is positively associated estrogen receptor positive breast cancers and improved prognosis (van 't Veer et al. (monash.edu)
  • We then used small interfering RNA-based MSI1 and MSI2 double knockdown (DKD) to understand gene expression and functional changes upon MSI depletion. (bvsalud.org)
  • Spiller B. O., Blackbourne, D., Mark L, Proctor D. and * Blom A. M. , (2003) Functional activity of the complement regulator encoded by Kaposi's sarcoma associated herpesvirus. (lu.se)
  • To this end, we set up a protocol to specifically enrich positive migration regulator candidates. (nature.com)
  • BTS is an iron-deficiency-induced gene whose transcript level increases in response to iron deficiency. (molcells.org)
  • The powerful high throughput screening approach, developed in my group, enables me to study all the possible combinations between a phenotypic reporter of choice and a collection of gene mutants throughout the yeast genome in a few months (while a conventional manual test would take more than several decades). (gu.se)
  • With advances in genome-wide gene expression technologies, classification of cancer subtypes based on expression signatures is widespread and results in many biomarkers for various cancers. (biomedcentral.com)
  • Studies in yeast have identified a series of autophagy- (ATG-) related genes forming the autophagy machinery. (hindawi.com)
  • These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
  • Using a microarray assay and the real time PCR, we further shown that mutation inhibited the manifestation of sialic acid synthesis gene, which correlated with the observation that mutation decreased the cellular sialic acid content material. (health-ground.com)
  • Collectively, our results suggest that DXS1 , DXR , GGPPS2 , PSY1 and LCYB genes were the most important genes for carotenoids accumulation, while PAL-1 , PAL-4 , 4CL-2 , 4CL-5 , CHS-1 and CHI for flavonoids biosynthesis. (researchsquare.com)
  • All cells must detect and respond to changes in their environment, often through changes in gene expression. (nih.gov)
  • Chilling stress or high BR levels decreased the abundance of BRASSINOSTEROID \INSENSITIVE2 (BIN2), a negative regulator of BR signaling. (sciencenet.cn)
  • Therefore, BTS is thought to be a negative regulator of the iron deficiency response. (molcells.org)
  • It has been proposed that upon iron deficiency, BTS is produced as a negative regulator together with those involved in iron uptake in order to prevent excess iron uptake that might happen due to over-activation of the uptake process in response to iron deficiency. (molcells.org)
  • Neuraminidase 1 is a negative regulator of lysosomal exocytosis. (medscape.com)
  • Some affected males have a mild mutation in one copy of the CFTR gene in each cell and a more severe, cystic fibrosis-causing mutation in the other copy of the gene. (medlineplus.gov)
  • Changes in the CFTR gene also have been associated with rhinosinusitis, which is a chronic inflammation of the tissues that line the sinuses. (medlineplus.gov)
  • What is the normal function of the CFTR gene? (experts123.com)
  • Quorum sensing is mediated by the accessory gene regulator agr , and RNAIII is the effector ( 9 ). (cdc.gov)
  • We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
  • With thousands of clinical trials to date, gene therapy is a flourishing strategy with great promise for the treatment of diseases impacting the nervous system. (frontiersin.org)
  • Preliminary studies indicate that both of these properties have positively correlated with the clinical subtype of sialidosis. (medscape.com)
  • The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
  • Expression of each cluster's gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq. (stowers.org)
  • This provides an exemplary case to pinpoint upstream regulators of known cancer signatures as cancer drivers and, accordingly, to a promising therapeutic target. (biomedcentral.com)
  • In this study, Cara cara navel orange fruit at four development stages were used to identify the key genes and TFs for carotenoids and flavonoids accumulation. (researchsquare.com)
  • In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
  • We demonstrate that our approach could identify upstream biomarkers for a given set of signature genes with markedly smaller size and comparable performances. (biomedcentral.com)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • A computational model of master regulators that are required for successful reprogramming the dynamics of this core network has revealed that it functions as of a differentiated cell into a cell exhibiting stem cell like a bistable switch, which in the on state, corresponds to all these properties. (lu.se)