Positive rna stability. Medical search. Frequent questions

A recently published study found that viral RNA was consistently detectable in blood of cynomolgus macaques until 3 weeks after euthanasia ( 6 ). (cdc.gov)
In addition, quantification (copies/mL) or cycle threshold (C t ) determination of EBOV RNA for estimating viral load has been shown to be a major prognosis marker in affected patients ( 1 , 7 , 8 ). (cdc.gov)
Moreover, C t value does not reflect viral viability but only presence of RNA in samples. (cdc.gov)
The foot-and-mouth disease virus (FMDV) capsid is highly acid labile and tends to dissociate into pentameric subunits at acidic condition to release viral RNA for initiating virus replication. (biomedcentral.com)
The generation of an infectious FMDV particle is a complex feat of engineering mainly involved in the process of capsid assembly, RNA encapsidation, and the viral maturation which are regulated by complicated events in infected cells. (biomedcentral.com)
Maybe the interaction mechanism between RNA molecule and viral capsid would help us solve this puzzle. (biomedcentral.com)
It is reasonable to assume that some viral messenger RNAs will trigger changes in the host miRNA transcription profiles or stabilities and that the resulting modified miRNA clusters might favor the development of different disorders. (ldsfreedomforum.com)
Results: The spiked ante- and postmortem sera from related donors showed similar results for HBsAg, anti-HBc, anti-HCV, HCV RNA, anti-HIV, and HIV p24 Ag, indicating a high stability of viral markers in cadaveric specimens. (uni-frankfurt.de)
In addition to molecular testing for the etiological diagnosis of COVID-19, which is mainly based on the detection of viral RNA using reverse transcriptase polymerase chain reaction techniques (rRT-PCR) on nasopharyngeal swabs, laboratory diagnostics may measure specific IgM, IgG and IgA classes antibodies against SARS-CoV-2 antigens. (medrxiv.org)
In the pediatrics, outpatient clinic waiting room, where the children were without masks, and unvaccinated, 66% of the samples were positive for viral RNA. (aaqr.org)
The viral RNA was positive in 50% of the COVID-19 patient rooms, 33% of the febrile disease outpatient clinic, and 33% of the clinical laboratory waiting room. (aaqr.org)
Only one sample collected 24 hours after the meeting was weakly positive for the viral RNA (1.12 × 10 2 PFU m -3 ). (aaqr.org)

Unlike such genetic changes, epigenetic changes, which do not alter genome sequence, can result in three possible outcomes: passive dilution, active repair through negative feedback, or active maintenance through positive feedback. (nature.com)
The virion consists of single-stranded positive-sense RNA genome of approximately 8500 nucleotides in length and icosahedron capsid protein [ 5 , 6 ]. (biomedcentral.com)
Here I review recent work in yeast, flies, mice and primates that examines the influences of naturally occurring sequence variation, chromosomal order and speciation on genome-wide expression profiles of both RNA and protein. (biomedcentral.com)
The RNA genome of SARS-CoV-2 consists of four proteins and 30000 nucleotides. (ijpsr.com)
These viruses are enveloped with a positive sense single-strand RNA genome 1, 6, 7 . (ijpsr.com)
Primary and secondary structure analysis showed that the RNA genome of the PSTVd-TR isolate (GenBank Accession No. HQ456944) differed from other compared isolates by only a few nucleotides. (tubitak.gov.tr)
These RNAs then target complementary DNA for elimination in a positive feedback process, contributing to germline defense and genome stability. (unibe.ch)

We propose an underlying mechanism for the favorable effect of longer DNA on gene expression in terms of the enhancement of access of RNA polymerase to the shrunken conformation. (nature.com)
Rifaximin binds to the beta-subunit of bacterial DNA-dependent RNA polymerase and prevents catalysis of polymerization of deoxyribonucleotides into a DNA strand, thereby inhibiting bacterial RNA synthesis. (hindawi.com)
In vitro synthesis of RNA requires a purified DNA plasmid, purified RNA polymerase, metal-ion enzyme cofactors, and raw-material nucleotides. (bioprocessintl.com)
We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
We examined the effects of SCD inhibition on Wnt signaling, including the expression and stability of low-density lipoprotein-receptor-related proteins 5 and 6 (LRP5 and LRP6), by immunoblot and quantitative polymerase chain reaction analyses. (nih.gov)
Specific mutations, characteristic of the A2a clade, were also detected, which included the P323L in RNA-dependent RNA polymerase and D614G in the Spike glycoprotein. (iisc.ac.in)

5- Different classes of non-coding RNAs such as small and micro RNAs can be involved in splicing, ribosome synthesis, degradation of mRNAs, translation repression. (france-genomique.org)

30, but in urine, RNA degradation occurred more quickly. (cdc.gov)
An ideal stability indicating analytical method is one that quantifies the drug and also resolves its degradation products. (hindawi.com)
The ICH guideline explicitly states the requirement of stability indicating methodology by conducting forced degradation studies under a variety of conditions, like pH, light, oxidation, dry heat, and so forth, and for separating drug from its degradation products, [ 3 , 13 ]. (hindawi.com)
Moreover, forced degradation studies are carried out to establish the inherent stability characteristics and degradation pathways under various conditions [ 14 ]. (hindawi.com)
Although stability indicating methods have been reported for assay of various drugs in drug products, chromatographic methods have taken precedence over the conventional methods of analysis, showing greater accuracy and sensitivity for small amounts of degradation products generated [ 13 , 16 ]. (hindawi.com)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Prevents RNA degradation thereby drastically reducing false positives. (filtrous.com)

RNA stability assay was applied to characterize the mechanism of C1QBP regulating XDH transcription. (medsci.org)
RNA in the completed transcription mixture is massively more abundant than the plasmid or enzymes. (bioprocessintl.com)
However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
a) genes whose products organize the cytoskeleton and establish the initial polarity of the embryo, (b) genes that encode cell signaling pathways, (c) genes whose products regulate mRNA translation and protein stability, and (d) genes that encode positive and negative regulators of transcription. (pewtrusts.org)
3- Regulatory proteins (transcription factors) have the ability to bind specifically to short DNA sequences and to regulate the transcription of a gene in a positive or negative way. (france-genomique.org)

The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. (nih.gov)
Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. (nih.gov)
The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. (nih.gov)
The current model of dissociation of FMDV supports the assumption that the acidification system in endosome triggers the direct dissociation of FMDV into pentameric subunits, protein VP4, and RNA without intermediate. (biomedcentral.com)
Researchers have found that dyskerin, a protein that provides stability to non-coding small nucleolar RNAs, signals when cutaneous squamous cell carcinomas are preparing to metastasize by migrating to the lymph nodes. (bio-itworld.com)
4- RNA-protein interactions (RNP: ribonucleoprotein complexes) act on post-trancriptional mechanisms such as splicing, RNA stability, etc. (france-genomique.org)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
Furthermore, PKP3 engages with the RNA-binding protein FXR1 to stabilize OTUB1 mRNA, and OTUB1 elevates PD-L1 abundance by facilitating its deubiquitination. (biomedcentral.com)
More importantly, the possible implications of mutation D614G (in SDÂ domain) and G1124V (in S2 subunit) on the structural stability of S protein have also been discussed. (iisc.ac.in)
This process allows the generation of a double-stranded RNA for Dicer-like protein cleavage to give rise to a population of small regulatory RNAs that precisely match the excised DNA sequences. (unibe.ch)
Positive regulators include two protein groups that allow cells to pass through regulatory checkpoints: cyclins and cyclin-dependent kinases (CDKs). (jove.com)

Wang M, Yu F, Wu W, Zhang Y, Chang W, Ponnusamy M, Wang K, Li P. Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity. (ijbs.com)
Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
Circular RNAs (circRNAs) have been implicated in cancer progression. (biomedcentral.com)

In vitro studies of rifaximin have demonstrated broad-spectrum coverage including gram-positive, gram-negative, and anaerobic bacteria as well as a limited risk of bacterial resistance [ 5 ]. (hindawi.com)
The conflict between the acid lability in vivo and acid stability in vitro of FMDV capsid promotes the selection of a series of amino acid substitutions which can confer resistance to acid-induced FMDV inactivation. (biomedcentral.com)

We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Some RNA samples, although positive by RT-PCR test, did not give a hybridization signal by dot blot assay. (tubitak.gov.tr)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
A luciferase reporter assay, RNA immunoprecipitation (RIP), RNA pulldown assays and fluorescence in situ hybridization were performed to identify the interactions among circIGF2BP3, miR-328-3p, miR-3173-5p and plakophilin 3 (PKP3). (biomedcentral.com)

miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)

Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)
levels of SCD messenger RNA (mRNA) correlated with HCC stage and patient survival time. (nih.gov)

short lengths of RNA produced from double-stranded RNA during the process of RNA interference. (freezingblue.com)
A powerful tool for our studies of embryogenesis involves a reverse genetic method called RNA interference or simply "RNAi. (pewtrusts.org)
This method is similar conceptually to "antisense" however the active agent is double stranded RNA and the interference effect is remarkably specific, potent and long lived. (pewtrusts.org)

Non-coding RNA (ncRNA) such as microRNA (miRNA) and long non-coding RNA (lncRNA) regulates the translation and/or stability of multiple mRNA targets either directly or by modulation of signal transduction pathways. (frontiersin.org)

This refers to species in which part of the sequence doubles back on itself and forms hydrogen-bonded bridges to complementary sequences on the same molecule and/or with other RNA molecules. (bioprocessintl.com)
The study of pH stability of FMDV not only contributes to the exploration of molecule and mechanism information for FMDV uncoating, but also enlightens the development of FMDV vaccines, including the traditionally inactivated vaccines and the new VLP (virus-like particle) vaccines. (biomedcentral.com)

Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. (nature.com)

RNA or DNA) sequences of interest in organisms present in a clinical specimen. (cdc.gov)

It must be absent from a final product to prevent triggering a toxic cytokine storm and to facilitate proper translation of the single-stranded RNA (ssRNA) ( 1 ). (bioprocessintl.com)
A piece of single-stranded nucleic acid that hybridizes specifically to the complementary sequence of RNA or DNA in the sample. (cdc.gov)

In Caenorhabditis elegans , RNA-dependent RNA polymerases (RdRPs) are used for the small RNA-guided production of additional small RNAs 8 that are complementary to terminally modified mRNA fragments 9 . (nature.com)

The C-nucleoside based drug Remdesivir has these necessary antiviral properties and is effective against a number of RNA viruses, including corona and ebola viruses. (tugraz.at)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
Alpha, beta, gamma, and delta are the four subgroups of coronavirus, which are the largest known spherical RNA viruses. (ijpsr.com)
Further environmental sampling studies of SARS-CoV-2 should consider including testing for the environmental presence of viruses within laboratory settings, targeting the collection of samples to early in the course of a patient's illness and including sampling of confirmed positive control surfaces, while maintaining appropriate biosafety measures. (who.int)

The first comprehensive vaccinations against COVID-19 with RNA vaccines have been running for a few days. (tugraz.at)
The biocatalytic production of C-nucleosides could provide further impetus for this new hope as well as RNA vaccines based on C-nucleosides. (tugraz.at)
RNA needs this kind of asset to make gene therapy and vaccines available to billions. (bioprocessintl.com)
This review summarized the molecular determinants of the acid stability of FMDV in order to explore the uncoating mechanism of FMDV and improve the acid stability of vaccines. (biomedcentral.com)
In order to explore the uncoating activity of FMDV and enhance the acid stability of vaccines, we summarized the available works about the pH stability of FMDV. (biomedcentral.com)

We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West Africa's environmental conditions. (cdc.gov)
Few laboratory studies have investigated Ebola virus RNA stability over time in collected samples. (cdc.gov)
We measured EBOV RNA stability in blood and urine samples from 7 case-patients with laboratory-confirmed Ebola. (cdc.gov)

Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)

Plasmid preparations can be contaminated with host DNA, RNA, and proteins. (bioprocessintl.com)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)

They have low stability but high mutation potential 4, 7, 8 . (ijpsr.com)

This facilitates the development of antiviral agents and RNA-based therapeutics. (tugraz.at)
GUILDFORD, United Kingdom, Sept. 18, 2023 (GLOBE NEWSWIRE) -- SiSaf Ltd, an RNA therapeutics company, is pleased to announce the publication in the peer-reviewed journal Molecular Therapy Nucleic Acids (1) of positive in vivo data demonstrating the safety and efficacy of its siRNA therapy for Autosomal Dominant Osteopetrosis 2 (ADO2). (wane.com)
SiSaf is an RNA therapeutics company with a proprietary delivery platform. (wane.com)

For example, although the presence of RNA amplification correlates with reported cases of persistent RNA silencing, most induced silencing dissipates within a few generations (reviewed in ref. 7 ). (nature.com)
A technique that amplifies (copies) DNA or RNA segments, in Amplification (NAA) order to directly identify microorganisms in sputum specimens. (cdc.gov)

In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. (qiagen.com)

It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. (nih.gov)
And it could have wider translational significance for RNA-based treatments of other skeletal disorders. (wane.com)

Comparable to a 'Trojan horse', it can be assumed that a whole series of metabolic events will be triggered as a positive feedback loop in any given gene network that is regulated by miRNAs. (ldsfreedomforum.com)
It is also known that most of the miRNAs that operate in the nucleus will simultaneously regulate transcript stability in the cytoplasm and vice versa, resulting in a highly integrated mechanism. (ldsfreedomforum.com)

This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans . (nature.com)

Methods: Twenty related and paired ante- and postmortem blood samples from cornea donors were obtained and subsequently analyzed for hepatitis B surface antigen (HBsAg), hepatitis B antibody (anti-HBc), anti-HCV, HCV RNA, anti-HIV-1/2, and HIV p24 Ag using Abbott test systems. (uni-frankfurt.de)
6. Active infection requiring therapy, including known positive tests for Hepatitis B surface antigen and hepatitis C virus (HCV) RNA. (who.int)

The FastLane Cell Probe Kit delivers sensitive probe-based detection in real-time one-step RT-PCR without the need for RNA purification. (qiagen.com)
Third, to assist in the diagnosis of patients who present later in their disease course or when the standard RNA detection methods are negative but clinical suspicion is high and other diseases have been ruled out [ 6 , 7 ]. (medrxiv.org)
Detection of severe acute respiratory syndrome coronavirus 2 RNA on surfaces in quarantine rooms. (who.int)

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), the virus causing coronavirus disease 2019 (COVID-19), is a positive-stranded RNA virus, similar to other coronaviruses. (medscape.com)

Natural and synthetic nucleosides have an antiviral effect and can act as building blocks of ribonucleic acids (RNA). (tugraz.at)

Co-author on the paper, Dr. Suzanne Saffie-Siebert, CEO of SiSaf Ltd, said, "Targeting the bone safely and effectively is a challenge for RNA-based therapies. (wane.com)

In the ciliated protozoan Paramecium tetraurelia, Piwi-associated small RNAs are generated upon the elimination of tens of thousands of short transposon-derived DNA segments as part of development. (unibe.ch)
In this work, we investigate the formation of these RNAs, which we show to be transcribed directly from the short (length mode 27 bp) excised DNA segments. (unibe.ch)
Our data support a mechanism whereby the concatenation and circularization of excised DNA segments provides a template for RNA production. (unibe.ch)
Surgical resection includes cholecystectomy, en bloc hepatic resection (typically of segments IVb and V), and lymphadenectomy with or without bile duct excision, depending on the location of the tumor. (medscape.com)

Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)

C t value, which can be used to perform RNA semiquantification, is not an absolute quantification, which necessitates use of a calibration curve. (cdc.gov)

cellular mechanism activated by double-stranded RNA molecules that results in the destruction of RNAs containing a similar nucleotide sequence. (freezingblue.com)

This cycle of small RNA production can act across generations, leading to effects that last for varying numbers of generations (Supplementary Table 1 ). (nature.com)
However, the mere presence of small RNAs (Supplementary Table 1 ) or terminally modified mRNA fragments 9 does not result in indefinite RNA silencing. (nature.com)

It allowed them to screen efficacy, stability, pharmacokinetics, and various aspects of manufacturability for hundreds of clones, then objectively select the best qualified clinical candidates, far in advance of commercial product development. (bioprocessintl.com)

In an additional series of 44 SARS-CoV-2 positive, IgM and IgG time kinetics were also evaluated in a time-period of 38 days. (medrxiv.org)

The HPV 16/18 was detected in 24% of the pterygium tissues (31 of 129) but not in the normal conjunctiva, and the HPV16/18 E6 oncoprotein was detected in 48.3% of HPV 16/18 DNA-positive pterygium tissues (15 of 31). (molvis.org)

This is particularly relevant for the production of RNA-based therapeutic products, as the incorporation of pseudouridine into the RNA increases stability and half-life and thus improves the effectiveness of therapeutic RNA, such as a vaccine. (tugraz.at)
However, the acid stability of virus capsid is greatly required for the maintenance of intact virion during the process of virus culture and vaccine production. (biomedcentral.com)
Is there anything in the vaccine that would suggest there may be other forms of RNA in it? (ldsfreedomforum.com)

CLIA-certified laboratories or testing sites are no longer required to report negative results for non-NAAT tests (rapid or antigen test results) or antibody tests, positive or negative. (nails-beauty.de)
A positive antigen test result is considered accurate when instructions are carefully followed. (nails-beauty.de)

They were also able to show that one of these derivatives can be incorporated into RNA and thus enable the modification of RNA. (tugraz.at)

Despite this, the mRNAs have concerns about their stability and immuno-stimulation, therefore, researchers and companies have concentrated their work to address this issue [2]. (researchgate.net)

One is that the RNA is packaged into the immature capsid after the arrangement of 12 copies of pentamer into a icosahedral capsid. (biomedcentral.com)

Interesting correlation was observed between these mutations and travel or contact history of COVID-19 positive cases. (iisc.ac.in)

HPV 16/18 E6 contributes to HPV-mediated pterygium pathogenesis as it is partly involved in p53 inactivation and is expressed in HPV DNA-positive pterygium. (molvis.org)

Anatomy5

Organisms5

Diseases1

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes50

Information Science3

Health Care2

Viral12

Genome7

Polymerase6

Synthesis1

Degradation7

Transcription6

Protein11

Circular RNAs4

Vitro2

Assay4

MRNA stability3

Messenger RNA2

Interference3

MicroRNA1

Molecule2

Coding sequence1

Sequences of interest1

Single-stranded2

Complementary1

Viruses4

Vaccines5

Ebola3

CircRNAs1

Proteins2

Mutation1

Therapeutics3

Amplification2

Stabilize1

Translational2

MiRNAs2

Homologous1

Hepatitis2

Detection3

Coronavirus1

Antiviral1

Therapies1

Segments4

Regulatory1

Quantification1

Mechanism1

Small2

Clinical1

Kinetics1

Tissues1

Vaccine3

Antigen2

Derivatives1

MRNAs1

Copies1

COVID1

Inactivation1