• The goal of this thesis was to characterize how cells regulate signaling of cytokinesis, both positively and negatively, during cell division using the fission yeast Schizosaccharomyces pombe as a model organism. (umassmed.edu)
  • A single intronless action gene in the fission yeast Schizosaccharomyces pombe: Nucleotide sequence and transcripts formed in homologous and heterologous yeast. (mpg.de)
  • Secretory production of ricinoleic acid in fission yeast Schizosaccharomyces pombe. (nih.gov)
  • Engineered high content of ricinoleic acid in fission yeast Schizosaccharomyces pombe. (nih.gov)
  • Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus . (biorxiv.org)
  • The fission yeast Schizosaccharomyces japonicus provides an attractive stripped-down model for metabolic compartmentalization in peroxisomes. (biorxiv.org)
  • Professor Forsburg studies how chromosome duplication and maintenance contributes to overall genome stability using a simple model genetic system, the fission yeast Schizosaccharomyces pombe . (usc.edu)
  • In the February 21 Nature, an international consortium of laboratories, led by the British Nobel laureate Paul Nurse, reports the complete sequence of the fission yeast Schizosaccharomyces pombe (Nature 2002, 415:871-880).The depth of sequence coverage was about eight-fold. (the-scientist.com)
  • In our studies, we employ a range of genetic, biochemical, cell biology, computational and genomic methods using predominantly the fission yeast Schizosaccharomyces pombe as a model. (google.com)
  • The secret behind BeBella's powerful anti-aging properties lies in the combination of nutrient-rich extracts harvested from fermentation of the probiotic Lactobacillus casei and fission yeast Schizosaccharomyces pombe . (prnewswire.com)
  • Cell division in many eukaryotes, including the fission yeast Schizosaccharomyces pombe, utilizes a contractile actomyosin ring. (thebiogrid.org)
  • The strain included a gene for malolactic enzyme from the bacterium Oenococcus oeni and a malate permease gene from the fission yeast Schizosaccharomyces pombe . (i-sis.org.uk)
  • An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. (thebiogrid.org)
  • In 1680, Dutch naturalist Anton van Leeuwenhoek first microscopically observed yeast, but at the time did not consider them to be living organisms, but rather globular structures as researchers were doubtful whether yeasts were algae or fungi. (wikipedia.org)
  • it induced gene conversion in yeast. (inchem.org)
  • In a new study in the Journal of Agricultural and Food Chemistry , Wang and coworkers were able to toughen up budding yeast against caffeine by adding bfr1 , a gene from Schizosaccharomyces pombe that encodes the ABC transporter that shunts caffeine out of the cell. (yeastgenome.org)
  • When Wang and coworkers added the gene to S. cerevisiae , this newly engineered yeast could now better tolerate caffeine. (yeastgenome.org)
  • Schizosaccharomyces pombe acid phosphatase (gene pho1). (embl.de)
  • The yeast ML01 was modified using a shuttle vector containing a chromosome integration cassette with genes for malolactic enzyme, malate transporter (permease), regulatory genes and a sequence directing homologous recombination at a chromosomal locus (not specified in the FDA report), and the antibiotic phleomycin gene was used as a selectable marker via another plasmid. (i-sis.org.uk)
  • The company distributing the GM yeast, Springer Oenologie, Lesaffre Group of North America, stated that the malate transporter gene and malolactic gene were both controlled by the phosphoglycerate kinase gene (PGK1) promoter and transcription terminator. (i-sis.org.uk)
  • In plants the recombinant gene insertions are not precise and disrupt genes that are not specifically targeted, while in yeast, gene insertions disrupt genes that are targeted. (i-sis.org.uk)
  • The expression vector comes equipped with a sequence homologous with a yeast chromosomal gene. (i-sis.org.uk)
  • Recombination between vector and chromosomal gene disrupts the target chromosomal gene and inserts the transgene, and frequently also a selectable marker, into the yeast target locus [3]. (i-sis.org.uk)
  • The yeast released for commercial wine production, ML01, was found to be only somewhat substantially equivalent to unmodified wine yeast, as a cytochrome p450 enzyme protein appeared to have been altered from the parental strain based on a comprehensive analysis of the yeast cell proteins, and a number of codon changes were observed in the inserted malolactic gene cassette, but those changes were not considered significant. (i-sis.org.uk)
  • The fate of yeast and plant DNA, as monitored by yeast chitinase gene and the plant chlorophyll a/b binding protein gene and micro-satellite markers showed that large DNA markers were present in must (the starting fermentation mixture of mainly yeast and grape juice), while the 250 base pair micro-satellites were present in both must and young wine for up to six months [9]. (i-sis.org.uk)
  • Fission yeast is a single-celled organism with chromosomes very similar to those in humans, and uses the same genes to maintain those chromosomes. (usc.edu)
  • Fission yeast is a simple, single-celled organism with chromosomes very similar to those in humans, and has proven to be an important model for cell division. (usc.edu)
  • Yeast constitutive and repressible acid phosphatases (genes PHO3 and PHO5). (embl.de)
  • After culturing the selected antibiotic resistant lactic-acid producing yeast, a phleomycin-sensitive lactic acid producing strain was isolated and found to contain the integrated malolactic-malate transporter genes. (i-sis.org.uk)
  • The shuttle vector may also replicate autonomously in the yeast nucleus and express genes equipped with promoter and terminator genes. (i-sis.org.uk)
  • Although hybrids of these two species inherit three chromosomes from each parent, the majority of spores (the yeast equivalent of sperm) that these hybrids produce fail to develop into new yeast cells. (elifesciences.org)
  • the other was due to three different sites in the DNA of S. kambucha that interfere with the development of the spores that inherit S. pombe chromosomes. (elifesciences.org)
  • The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. (wikipedia.org)
  • Some yeast species have the ability to develop multicellular characteristics by forming strings of connected budding cells known as pseudohyphae or false hyphae, or quickly evolve into a multicellular cluster with specialised cell organelles function. (wikipedia.org)
  • Yeast sizes vary greatly, depending on species and environment, typically measuring 3-4 µm in diameter, although some yeasts can grow to 40 µm in size. (wikipedia.org)
  • The yeast species Saccharomyces cerevisiae converts carbohydrates to carbon dioxide and alcohols through the process of fermentation. (wikipedia.org)
  • Other species of yeasts, such as Candida albicans, are opportunistic pathogens and can cause infections in humans. (wikipedia.org)
  • Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
  • have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
  • Since these two yeast species are so closely related, the findings of Zanders et al. (elifesciences.org)
  • It also contains a range of yeast and bacterial species with polyphenols, amino acids, and organic acids. (organicfacts.net)
  • In an effort to understand the fundamental signaling elements, or 'core machinery', required for the function of these GTPases, we describe here the conservation and functional similarities of Cdc42 and Rac effectors in five different species: plant, yeast, fruit fly, roundworm and human. (biomedcentral.com)
  • We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
  • The modification of bacteria and yeast is based on homologous recombination while modification of plants is based on illegitimate recombination. (i-sis.org.uk)
  • Yeast cells in wine were found to be hyperactive in mitotic recombination, contributing to the observed instability of wine yeasts [7]. (i-sis.org.uk)
  • In the paper "Mémoire sur la fermentation alcoolique," Pasteur proved that alcoholic fermentation was conducted by living yeasts and not by a chemical catalyst. (wikipedia.org)
  • Wine making involves alcoholic fermentation via the metabolic pathways of yeast and the malolactic pathway to convert malic acid to lactic acid, to reduce the acidity of the wine. (i-sis.org.uk)
  • Most yeasts reproduce asexually by mitosis, and many do so by the asymmetric division process known as budding. (wikipedia.org)
  • We conclude that signaling cytokinesis is tightly coordinated with mitosis in S. pombe by positive signals acting through Sid1p and the SIN, and under certain conditions, negative signals acting through Dma1p. (umassmed.edu)
  • In S. pombe, the actomyosin ring is assembled at the medial cortex upon entry into mitosis and constricts at the end of anaphase to guide the centripetal deposition of the septum. (thebiogrid.org)
  • Nurse P. Genetic control of cell size at cell division in yeast. (harvard.edu)
  • Maybe this genetic engineered yeast can make the equivalent of a Red Bull and vodka all in one step! (yeastgenome.org)
  • Genetic modification of yeast and bacteria differs fundamentally from modification of plants. (i-sis.org.uk)
  • For yeast genetic engineering 'shuttle' vectors are used, which are propagated in bacteria for insertion in yeast. (i-sis.org.uk)
  • In 2003, the United States Food and Drug Administration (FDA) designated the GM yeast strain Saccharomyces cerevisae ML01 'generally recognized as safe' (GRAS). (i-sis.org.uk)
  • Of course unlike even the most grizzled 30 year coffee drinker with ideal genetics, the yeast almost certainly gets no joy from its morning Joe. (yeastgenome.org)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
  • Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis. (thebiogrid.org)
  • The original anti-phleomycin plasmid did not contain a sequence allowing it to be integrated into the yeast chromosome and the plasmid was therefore unstable and frequently lost from the yeast cell [1]. (i-sis.org.uk)
  • Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. (wikipedia.org)
  • ATLANTA , April 28, 2015 /PRNewswire/ -- Today, a new skincare company BeBella, LLC announced its launch of a line of anti-aging products based on a unique probiotic and fission yeast technology. (prnewswire.com)
  • Kombucha is fermented with a live colony of bacteria and yeast, so it works as a probiotic by sustaining gut health and provides relief from various abdominal disorders including chronic constipation and diarrhea . (organicfacts.net)
  • In fact, like a lot of other microorganisms, caffeine actually kills this yeast. (yeastgenome.org)
  • Yeasts have recently been used to generate electricity in microbial fuel cells and to produce ethanol for the biofuel industry. (wikipedia.org)
  • Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe. (nih.gov)
  • Description: English: Division stages of Schizosaccharomyces pombe in bright field (left) and dark field (right) light microscopy (Zeiss Axiostar, 100x objective, oil immersion), compound picture Deutsch: Teilungssatdien von Schizosaccharomyces pombe im Hellfeld (links) und Dunkelfeld (rechts) (Zeiss Axiostar, 100x Objective, Ölimmersion), zusammengesetztes Bild. (eol.org)
  • Oral administration of whole dihomo-γ-linolenic acid-producing yeast suppresses allergic contact dermatitis in mice. (nih.gov)
  • We show that the first of the two transition points of cdc2.33, a mutant of Schizosaccharomyces pombe, exists in exponential phase cells. (ox.ac.uk)
  • While not as robust as bfr1-B , this mutant allowed yeast expressing it to tolerate caffeine concentrations up to 15 mg/ml, conditions under which cells with wild type bfr1 failed to grow. (yeastgenome.org)
  • And who knows, maybe they can have the yeast make caffeine and alcohol at the same time creating the equivalent of a vodka and Red Bull in a single step! (yeastgenome.org)
  • Vessels studied from several archaeological sites in Israel (dating to around 5,000, 3,000 and 2,500 years ago), which were believed to have contained alcoholic beverages (beer and mead), were found to contain yeast colonies that had survived over the millennia, providing the first direct biological evidence of yeast use in early cultures. (wikipedia.org)
  • You can imagine adding the caffeine pathway from coffee to yeast and having the yeast merrily exporting caffeine to the culture medium where it can be harvested. (yeastgenome.org)
  • Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast. (thebiogrid.org)
  • Although the yeast don't get any of the pleasurable effects of caffeine, at least they can now happily grow in cultures that have more caffeine than a strong cup of coffee. (yeastgenome.org)
  • Kombucha tea is a refreshing beverage made by the fermenting of sweetened tea with a symbiotic combination of acetic acid, bacteria, and specific yeast cultures. (organicfacts.net)
  • Description: English: Visualization of yeast cells (Schizosaccharomyces pombe). (eol.org)
  • Heart muscle cells die en masse after injury, yet the adult mammalian heart retains little capacity to regenerate them. (natureasia.com)
  • To download a certificate of analysis for Schizosaccharomyces pombe Lindner ( MYA-230 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Schizosaccharomyces pombe Lindner ( MYA-230 ) is not currently available online. (atcc.org)
  • The certificate of analysis for that lot of Schizosaccharomyces pombe Lindner ( 44149 ) is not currently available online. (atcc.org)
  • Description: English: Cell size of the fission yeast across the cell cycle. (eol.org)
  • In the United States, naturally occurring airborne yeasts were used almost exclusively until commercial yeast was marketed at the Centennial Exposition in 1876 in Philadelphia, where Charles L. Fleischmann exhibited the product and a process to use it, as well as serving the resultant baked bread. (wikipedia.org)
  • Numerous translocations have been observed uniquely in wine yeasts and such chromosome rearrangements involving transgenes can lead to unexpected toxicity in the final product [8]. (i-sis.org.uk)
  • Description: English: Light microscopy of Schizosaccharomyces octosporus showing the ascospores with protective casings. (eol.org)
  • Description: English: An overview of the fission yeast general cell cycle. (eol.org)
  • English: Combined transmission electron microscopic images of Schizosaccharomyces pombe and its centrosome (spindle pole body). (eol.org)
  • Yeasts do not form a single taxonomic or phylogenetic grouping. (wikipedia.org)
  • Archaeologists digging in Egyptian ruins found early grinding stones and baking chambers for yeast-raised bread, as well as drawings of 4,000-year-old bakeries and breweries. (wikipedia.org)
  • Cdc42 and/or Rac homologs are found in fruit flies, round-worms and budding yeast. (biomedcentral.com)
  • For example, whereas wild type yeast barely grew with 8 mg/ml caffeine, the engineered yeast did OK. (yeastgenome.org)
  • With their single-celled growth habit, yeasts can be contrasted with molds, which grow hyphae. (wikipedia.org)
  • Previous research had shown that bfr1 was an important player in helping S. pombe deal with caffeine. (yeastgenome.org)
  • Neither the FDA document [1] nor the publications on ML01 [4] considered important complications associated with wine yeast. (i-sis.org.uk)
  • The fission yeast clade has lost the peroxisomal FA β-oxidation system. (biorxiv.org)