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  • gene
  • Immunoprecipitation experiments using antibodies to the TFB1 gene product demonstrate that the transcriptional and CTD kinase activities of factor b are closely associated with an oligomer of the three polypeptides. (sciencemag.org)
  • binds
  • Rho factor binds to the transcription terminator pause site, an exposed region of single stranded RNA (a stretch of 72 nucleotides) after the open reading frame at C-rich/G-poor sequences that lack obvious secondary structure. (wikipedia.org)
  • promoter
  • Interacting selectively and non-covalently with a member of the class of TATA-binding proteins (TBP), including any of the TBP-related factors (TRFs), to facilitate the aggregation, arrangement and bonding together of proteins on RNA polymerase II promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription by RNA polymerase. (yeastgenome.org)
  • enzyme
  • In short, Rho factor acts as an ATP-dependent unwinding enzyme, moving along the newly forming RNA molecule towards its 3′ end and unwinding it from the DNA template as it proceeds. (wikipedia.org)
  • proteins
  • The binding sites for these initiator proteins overlap with the binding sites for the DNA polymerase, so the clamp cannot simultaneously associate with a clamp loader and with a polymerase. (wikipedia.org)
  • DNA clamps also associate with other factors involved in DNA and genome homeostasis, such as nucleosome assembly factors, Okazaki fragment ligases, and DNA repair proteins. (wikipedia.org)
  • The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. (wikipedia.org)
  • subunits
  • Under some conditions, some of the 70-kDa subunits are replaced by one of the other, more-specific factors. (wikipedia.org)
  • In bacteria, the sliding clamp is a homodimer composed of two identical beta subunits of DNA polymerase III and hence is referred to as the beta clamp. (wikipedia.org)
  • The gamma complex of DNA polymerase III, composed of γδδ'χψ subunits, catalyzes ATP to chaperone two beta subunits to bind to DNA. (wikipedia.org)
  • The beta subunits in turn bind the αε polymerase complex. (wikipedia.org)
  • synthesis
  • because one of the rate-limiting steps in the DNA synthesis reaction is the association of the polymerase with the DNA template, the presence of the sliding clamp dramatically increases the number of nucleotides that the polymerase can add to the growing strand per association event. (wikipedia.org)
  • The presence of the DNA clamp can increase the rate of DNA synthesis up to 1,000-fold compared with a nonprocessive polymerase. (wikipedia.org)
  • terminator
  • But a nonsense mutation releases the ribosome, so that Rho is free to attach to and/or move along the RNA, enabling it to act on RNA polymerase at the terminator. (wikipedia.org)
  • gene
  • Immunoprecipitation experiments using antibodies to the TFB1 gene product demonstrate that the transcriptional and CTD kinase activities of factor b are closely associated with an oligomer of the three polypeptides. (sciencemag.org)
  • chain
  • Methods: Genomic DNA was extracted from 50 children under 15 years of age with ALL and 50 age-matched healthy children's whole blood and genetic variation of each participant was detected using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) with taqI restriction enzyme. (ijbc.ir)
  • 510 of these identified hCONDELs were then validated computationally with 39 of these being validated by polymerase chain reaction(PCR). (wikipedia.org)
  • The number of hCONDELs currently identified is 583 using the genome-wide comparison method however validation of these predicated regions of deletions through polymerase chain reaction methods produce 510 hCONDELs. (wikipedia.org)
  • human
  • Histoplasmosis in patients with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS): multicenter study of outcomes and factors associated with relapse," Medicine , vol. 93, no. 1, pp. 11-18, 2014. (hindawi.com)
  • important factor
  • These data indicate that the novel S68 Delta is a previously unrecognized deletion that may represent an important factor in NRTI multidrug resistance treatment strategies. (diva-portal.org)
  • influence
  • We also aimed to evaluate the influence of socio-economic factors, such as access to medical services, age, origin and education on the prevalence of certain infectious diseases. (biomedcentral.com)