Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). (wikipedia.org)
In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. (wikipedia.org)
The processive polyadenylation complex in the nucleus of eukaryotes works on products of RNA polymerase II, such as precursor mRNA. (wikipedia.org)
Cpeb1b-mediated cytoplasmic polyadenylation of mRNA modulates zebrafish definitive hematopoiesis. (stembook.org)
Messenger RNA (mRNA) polyadenylation is an essential cellular process in eukaryotes, which consists of cleavage at the 3′ end of pre-mRNA and an addition of a tract of adenosines [poly(A) tail]. (biomedcentral.com)
In vitro and in vivo experiments demonstrate that excess UIA protein specifically inhibits polyadenylation of pre-mRNAs that contain the conserved 3′ UTR from human UlA mRNA. (researchwithrutgers.com)
Thus, U1 A protein regulates the production of its own mRNA via a mechanism that involves pre-mRNA binding and inhibition of polyadenylation. (researchwithrutgers.com)
One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor or as well as for the tail elongation reaction. (unibas.ch)
In mice that were gene-edited to contain human STMN2 cryptic splice-polyadenylation sequences, ASO injection into cerebral spinal fluid successfully corrected Stmn2 pre-mRNA misprocessing and restored stathmin-2 expression levels independently of TDP-43 binding. (jax.org)
Alternative polyadenylation (APA) is a common post-transcriptional process of eukaryotic mRNA maturation, which generates altered 3′ ends of transcripts. (biomedcentral.com)
The candidates include a large number of essential proteins such as four canonical mRNA cleavage and polyadenylation factors. (nih.gov)
Inactivation of the pre-mRNA cleavage and polyadenylation factor Pfs2 in fission yeast causes lethal cell cycle defects. (ox.ac.uk)
The product of the orthologous Saccharomyces cerevisiae gene PFS2 is a component of a multiprotein complex required for 3'-end cleavage and polyadenylation of pre-mRNAs and, in keeping with the conservation of this essential function, an S. pombe pfs2 mutant was defective in mRNA 3'-end processing. (ox.ac.uk)
10. A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex. (nih.gov)
Polyadenylation involves recognizing the processing site signal, (AAUAAA), and cleaving of the mRNA to create a 3' OH terminal end to which poly A polymerase ( POLYNUCLEOTIDE ADENYLYLTRANSFERASE ) adds 60-200 adenylate residues. (bvsalud.org)
Cytoplasmic polyadenylation2
The expression and localization of cytoplasmic polyadenylation element binding proteins in the retina. (louisville.edu)
This involves the use of cytoplasmic polyadenylation binding proteins (CPEBs) to regulate translation. (louisville.edu)
Post-transcriptional2
Alternative polyadenylation (APA), which is regulated by both cis-elements and trans-factors, plays an important role in post-transcriptional regulation of eukaryotic gene expression. (mdc-berlin.de)
Alternative PolyAdenylation (APA) is a key post-transcriptional regulatory mechanism that varies the 3'UTR length of the majority of mRNAs, leading to modification of transcript stability, cellular localisation, and translation efficiency. (edu.au)
RNAs2
Dattaroy, T & Hunt, AG 2002, ' Polyadenylation of RNAs associated with a nucleus-localized phosphorolytic nuclease ', Journal of Plant Biochemistry and Biotechnology , vol. 11, no. 1, pp. 21-25. (uky.edu)
TDP-43 mislocalization results in cryptic splicing and polyadenylation of pre-messenger RNAs (pre-mRNAs) encoding stathmin-2 (also known as SCG10), a protein that is required for axonal regeneration. (jax.org)
Proteins1
Efficient in vitro and in vivo systems are now in place to study the role of viral proteins in replication and/or transcription, the regulation of these processes, polyadenylation of viral mRNAs, the viral promoter structures, or the significance of noncoding regions for virus replication. (ox.ac.uk)
Alternative22
Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing. (wikipedia.org)
These quantitative maps of polyA usage in evolutionarily and functionally related samples constitute a resource for understanding the regulatory mechanisms underlying alternative polyadenylation. (nih.gov)
Alternative polyadenylation (APA) has emerged as a pervasive mechanism that contributes to the transcriptome complexity and dynamics of gene regulation. (biomedcentral.com)
Alternative polyadenylation (APA), a posttranscriptional mechanism of gene expression via determination of 3′UTR length, has an emerging role in carcinogenesis. (jci.org)
Alternative cleavage and polyadenylation are emerging as an important layer of gene regulation as they generate transcript isoforms that differ in their 3' UTRs, thereby modulating genes' response to 3' UTR-mediated regulation. (prinsesmaximacentrum.nl)
However, mechanisms that regulate this enhanced alternative polyadenylation are unknown. (prinsesmaximacentrum.nl)
RESULTS: Here, we explored, on a transcriptome-wide scale, alternative polyadenylation events associated with cellular proliferation and neoplastic transformation. (prinsesmaximacentrum.nl)
CONCLUSIONS: Our results comprehensively identify alternative polyadenylation events associated with cellular proliferation and transformation, and demonstrate that the enhanced alternative polyadenylation in proliferative conditions results not only in global 3' UTR shortening but also in enhanced premature cleavage in introns. (prinsesmaximacentrum.nl)
Our results also indicate that E2F-mediated co-transcriptional regulation of 3'-end processing genes is one of the mechanisms that links enhanced alternative polyadenylation to proliferation. (prinsesmaximacentrum.nl)
However, mechanisms that regulate this enhanced alternative polyadenylation are unknown.RESULTS: Here, we explored, on a transcriptome-wide scale, alternative polyadenylation events associated with cellular proliferation and neoplastic transformation. (prinsesmaximacentrum.nl)
Alternative polyadenylation (APA) is a widespread and important mechanism in regulation of gene expression. (jci.org)
Genome-wide change of polyadenylation (polyA) sites (also known as alternative polyadenylation, APA) is emerging as an important strategy of gene regulation in response to stress in plants. (biomedcentral.com)
VEZF1-guanine quadruplex DNA interaction regulates alternative polyadenylation and detyrosinase activity of VASH1. (nih.gov)
1. Aberrant Alternative Polyadenylation is Responsible for Survivin Up-regulation in Ovarian Cancer. (nih.gov)
4. The microRNA target site landscape is a novel molecular feature associating alternative polyadenylation with immune evasion activity in breast cancer. (nih.gov)
6. Dynamics of alternative polyadenylation in human preimplantation embryos. (nih.gov)
8. Alternative Polyadenylation of ABC Transporters of the C-Family (ABCC1, ABCC2, ABCC3) and Implications on Posttranscriptional Micro-RNA Regulation. (nih.gov)
13. Detecting alternative polyadenylation from microarray data. (nih.gov)
16. Alternative polyadenylation of single cells delineates cell types and serves as a prognostic marker in early stage breast cancer. (nih.gov)
19. Alternative Polyadenylation in Triple-Negative Breast Tumors Allows NRAS and c-JUN to Bypass PUMILIO Posttranscriptional Regulation. (nih.gov)
20. Tempo-spatial alternative polyadenylation analysis reveals that 3' UTR lengthening of Mdm2 regulates p53 expression and cellular senescence in aged rat testis. (nih.gov)
One of the most exciting aspects of transcriptome biology is the contextual adaptability of eukaryotic transcriptomes and proteomes by alternative splicing (AS), alternative polyadenylation (APA), and alternative transcription start sites (ATSS), jointly referred to here as alternative transcript expression mechanisms (AltTEM). (biomedcentral.com)
Transcription5
The process of polyadenylation begins as the transcription of a gene terminates. (wikipedia.org)
In nuclear polyadenylation, a poly(A) tail is added to an RNA at the end of transcription. (wikipedia.org)
Orthomyxovirus replication, transcription, and polyadenylation. (ox.ac.uk)
and a transcription termination and polyadenylation signal. (genomeweb.com)
βGlo pAn: The human beta-globin 3'UTR and polyadenylation sequence allow the efficient arrest of the transgene transcription. (invivogen.com)
AAUAAA5
This site often has the polyadenylation signal sequence AAUAAA on the RNA, but variants of it that bind more weakly to CPSF exist. (wikipedia.org)
Most human polyadenylation sites contain the AAUAAA sequence, but this sequence is less common in plants and fungi. (wikipedia.org)
For any given mutation, cleavage and polyadenylation are reduced to similar extents, strongly suggesting that the same factor interacts with AAUAAA in both reactions. (nih.gov)
We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. (unibas.ch)
La poliadenilación requiere el reconocimiento de la señal del punto de proceso (AAUAAA), y la escisión del ARNm para crear un terminal OH en 3' al que la poli A (POLINUCLEOTIDO ADENILILTRANSFERASA) pueda unir 60-200 residuos de adenilato. (bvsalud.org)
Mechanism1
Mechanism of STMN2 cryptic splice-polyadenylation and its correction f" by Michael W Baughn, Ze'ev Melamed et al. (jax.org)
Genome-wide3
Here, we performed a genome-wide profiling of polyadenylation sites under heat and cold treatments in Populus trichocarpa . (biomedcentral.com)
Genome-Wide Analysis of Polyadenylation Events in Schmidtea mediterranea. (ncbs.res.in)
In this study we performed first genome-wide screening of RNApolyadenylation (pA) for KSHV transcripts in KSHV-infected B cells by RNA-seq technology. (nih.gov)
Sequence3
The polyadenylation signal - the sequence motif recognised by the RNA cleavage complex - varies between groups of eukaryotes. (wikipedia.org)
The system was used to examine the influence of 5'-capping, 3'-polyadenylation and leader sequence upon the translation and stability of the synthetic Tn9 cat (chloramphenicol acetyl transferase), yeast PGK (phosphoglycerate kinase) and yeast HSP26 (heat-shock protein 26) mRNAs. (kent.ac.uk)
and (d) a polyadenylation sequence positioned downstream of the insertion site. (nih.gov)
Subunit1
In the oxt6 mutant, a transfer DNA is inserted in the CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30) gene, which encodes a polyadenylation factor subunit homolog. (edu.sa)
PolyA2
We developed PolyA-seq, a strand-specific and quantitative method for high-throughput sequencing of 3' ends of polyadenylated transcripts, and used it to globally map polyadenylation (polyA) sites in 24 matched tissues in human, rhesus, dog, mouse, and rat. (nih.gov)
The use of different polyadenylation site (polyA) has been found to play essential roles of increasing transcriptome diversity and regulating gene expression profiles [ 1 , 2 ]. (biomedcentral.com)
Posttranscriptional1
Posttranscriptional polyadenylation of nascent transcripts plays a critical role in regulation of eukaryotic expression. (nih.gov)
Factors1
The thick rectangular frame encompasses the Hfq and PABP II polyadenylation stimulatory factors. (nih.gov)
Degradation3
In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. (wikipedia.org)
Institut de biologie moléculaire des plantes » Polyadenylation-assisted RNA degradation processes in plants. (cnrs.fr)
Polyadenylation-assisted RNA degradation processes in plants. (cnrs.fr)
Signal2
Gene Editing Targeting the DUX4 Polyadenylation Signal: A Therapy for FSHD? (mdpi.com)
hEF-1alpha pAn: This provides a strong polyadenylation signal. (invivogen.com)
Regulatory1
By analysis of sequences flanking pA sites we identified several regulatory cis-elements governing viral polyadenylation. (nih.gov)
Transcripts1
We also detected polyadenylation of noncoding and antisense transcripts, including constitutive and tissue-specific primary microRNAs. (nih.gov)
MRNAs1
However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. (wikipedia.org)
Sites3
Promoters are indicated with arrows and 'P'. The early and the two late polyadenylation sites are indicated with arrows and vertical bars on the genome map. (medscape.com)
Among the 24,721 polyadenylation sites (pAs) from 7,271 genes expressing multiple pAs, we identified 3,747 pAs showing significant divergence between the two strains. (mdc-berlin.de)
The use of alternate polyadenylation sites has been found for this gene. (nih.gov)
Genes1
We also looked at how these SINEs were importing polyadenylation signals into the 3'UTRs of dog genes. (jax.org)
Site1
The polymorphism results from a single A-T to G-C transition in the 3' noncoding region about 300 bp upstream from the polyadenylation site. (cdc.gov)
Factor2
Bercovich, N. The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30. (uba.ar)
Vazquez, M.P. 'The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30' (2009) Biochemical and Biophysical Research Communications. (uba.ar)
Tail1
The PABPN1 protein helps add adenines to the poly(A) tail through a process called polyadenylation. (medlineplus.gov)
Plays1
These results suggest a role for polyadenylation in exosome function, perhaps analogous to the role that polyadenylation plays in facilitating RNA breakdown by the bacterial degradosome. (uky.edu)
Similar1
Interestingly, RNApolyadenylation has been implicated in degradosome functioning, giving rise to the possibility of a similar role in exosome function. (uky.edu)
Global1
This work provides a comprehensive picture of global polyadenylation patterns in response to temperatures stresses in trees. (biomedcentral.com)