• nucleotides
  • If there is sufficient DNA to measure both mass and average length, it is possible to determine the proper amount of dATP to be added to generate poly(dA) tails 90 to 200 nucleotides long. (wikipedia.org)
  • The 5' terminus of the splint is designed to be complementary to the 3' end of the mRNA, and the 3' terminus of the splint is designed to be complementary to the 5' end of the DNA spacer linker, which usually consists of poly dA nucleotides (Figure 2). (wikipedia.org)
  • tail
  • To be compatible with the oligo(dT)50 primer on the flow cell surface, it is necessary to generate a poly(dA) tail of at least 50 nt at the 3' end of the molecule to be sequenced. (wikipedia.org)
  • Generation of a 3' poly(dA) tail can be accomplished with a variety of different ligases or polymerases. (wikipedia.org)
  • Hybridization to the flow cell is typically carried out at 55◦C for 1 hr.[citation needed] Generally, samples for sequencing are prepared in such a way that the poly(A) tail is longer than the oligo(dT)50 on the surface of the flow cell. (wikipedia.org)
  • Thus, all of the unpaired dAs present in the poly(A) tail are filled in with TTP. (wikipedia.org)
  • Messenger RNA is then purified using an oligo-dT column that binds to the poly-A tail, a signal specifically found on transcribed genes. (wikipedia.org)
  • bases
  • Previous investigations of poly(dA) have suggested that adenine bases stack in a very uniform way," said Ching-Hwa Kiang, a co-author of the new study and assistant professor of physics and astronomy at Rice. (redorbit.com)
  • Low complexity - complexity score of a sequence reflects number of identical bases in a series (homo-polymers) such as poly-dT or poly-dA. (wikipedia.org)
  • University
  • The new study on poly(dA) was conducted by Kiang, Rice graduate student Wuen-shiu Chen and colleagues at Rice and National Chung Hsing University (NCHU) in Taiwan. (redorbit.com)
  • The University of Madeira (UMa - Portuguese: Universidade da Madeira, pronounced [univɨɾsiˈdad(ɨ) dɐ mɐˈdɐjɾɐ], is a Portuguese public university, created in 1988 in Funchal. (wikipedia.org)
  • pinyin: Běijīng Gōngyè Dàxué), also called Beijing Polytechnic University or Bei Gong Da (北工大), is recognized as one of the Project 211 universities. (wikipedia.org)
  • Length
  • In addition to providing flexibility and length, the poly dA portion of the linker also allows further purification of the mRNA-polypeptide fusion due to its high affinity for dT cellulose resin. (wikipedia.org)