Pluripotent hematopoietic stem cells adherent cells cultured. Medical search. Frequent questions

While the terms mesenchymal stem cell (MSC) and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. (tno.nl)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are potentially unlimited cell sources for cellular therapies due to the unique capacities of PSCs to self-renew indefinitely and differentiate into cells from all three germ lineages (ectoderm, mesoderm, and endoderm) [ 1 ]. (biomedcentral.com)
The use of hASCs for therapeutic applications has grown substantially in the last years, because the use of stem cells from adult tissues circumvent some ethical issues associated with the application of embryonic stem cells, and because of their accessibility via isolation from lipoaspirates, a disposable byproduct of cosmetic surgery. (biomedcentral.com)
Although HECs are thought to be primarily limited to the aorta-gonad-mesonephros (AGM) during early development, EHT has been described in various other hematopoietic organs and embryonic vessels. (bu.edu)
Nanog is a unique homeobox transcription factor required to maintain the self-renewal and pluripotency of embryonic stem cells (ESCs). (researchsquare.com)
In addition, we found that in the presence of LIF, MDLS could replace feeder cells to maintain the undifferentiated state of OG2-mES cells (Oct4-GFP reporter gene mouse embryonic stem cell line), and the MDLS-expanded OG2-mES cells showed an elevated expression levels of pluripotency markers in vitro. (biomedcentral.com)
It is expressed in ESCs, embryonic germ (EG) cells and embryonic carcinoma (EC) cells and essential for early embryonic development [ 14 , 15 ]. (biomedcentral.com)
The influence of medium change on dissolved oxygen (DO) at cultivation of human embryonic stem cells (hESC) was investigated at different oxygen tensions in the incubator atmosphere. (presens.de)
Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)

Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (wikipedia.org)
Mesenchymal stem cells (MSCs), a term first coined by Arnold I. Caplan in 1991, are characterized morphologically by a small cell body with a few cell processes that are long and thin. (wikipedia.org)
However, MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
These cells have several advantages over bone-marrow-derived MSCs. (wikipedia.org)
MSCs have been seen to even differentiate into neuron-like cells, but doubt remains about whether the MSC-derived neurons are functional. (wikipedia.org)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Mesenchymal stem cells (MSCs) from bone marrow play a critical role in osteochondral repair. (hindawi.com)
Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. (hindawi.com)
Mesenchymal stem cells (MSCs) play a key role in articular cartilage repair. (hindawi.com)
MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
In contrast to the cost- and labor-intensive cultivation and propagation of cells such as MSCs or articular chondrocytes, the clinical use of "minimally processed" autologous BMA that can be prepared in the operation room as a single-step procedure appears straightforward. (hindawi.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
On infusion, culture-expanded MSCs regulate inflammatory and immune responses and tissue repair. (bmj.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
Mesenchymal stem cells (MSCs) are a promising cell type for cell-based therapies. (bvsalud.org)
Amniotic epithelial cells (AECs) and mesenchymal stem cells (MSCs) were isolated from placenta (a source of neonatal stem cells) by explant culture method. (bvsalud.org)
Results indicated that placenta-derived AECs and MSCs were efficiently differentiated into skin cells, that is, keratinocytes and fibroblasts, respectively, as indicated by morphological changes, immunostaining, and polymerase chain reaction analysis. (bvsalud.org)
Mesenchymal stem cells (MSCs) are an attractive candidate for autologous cell therapy, but their ability to repair damaged myocardium is severely compromised with advanced age. (bvsalud.org)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
MSCs were isolated from human umbilical cord (UC-MSCs) and expanded in an in vitro culture system. (biomedcentral.com)
UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)
Senescence of mesenchymal stem cells (MSCs) and the related functional decline of osteogenesis have emerged as the critical pathogenesis of osteoporosis in aging. (thno.org)

The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes and chondrocytes as well as myocytes. (wikipedia.org)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
Controlled expansion and differentiation of pluripotent stem cells (PSCs) using reproducible, high-throughput methods could accelerate stem cell research for clinical therapies. (biomedcentral.com)
This article discusses the challenges and opportunities of hydrodynamic culture environments for the expansion and differentiation of PSCs in microfluidic systems and larger-volume suspension bioreactors. (biomedcentral.com)
Ultimately, an improved understanding of the effects of hydrodynamics on the self-renewal and differentiation of PSCs could yield improved bioprocessing technologies to attain scalable PSC culture strategies that will probably be requisite for the development of therapeutic and diagnostic applications. (biomedcentral.com)
low cell number in adult tissues, restricted expansion and differentiation capacity, and donor-related heterogeneity limit their use. (bvsalud.org)
Cell viability and differentiation capacity toward the mesenchymal lineages were assessed, along with immunophenotype. (biomedcentral.com)
Malignant GEP-NET cells expressed genes and regulons associated with normal, gastrointestinal endocrine cell differentiation, and fate determination stages. (bu.edu)
However, the limited amount of stem cells and their tendency to undergo spontaneous differentiation upon extended propagation in vitro restrict their practical application. (biomedcentral.com)
Furthermore, MDLS could promote teratoma formation and enhanced differentiation potential of P19 cells in vivo. (biomedcentral.com)
that critically influences cell proliferation, differentiation and apoptosis ( Miele and Osborne, 1999 ). (elifesciences.org)
Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (thermofisher.com)

However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
Previous studies have shown that it is closely linked with cancer stem cells (CSCs), which have self-renewal potential and the capacity to differentiate into diverse populations. (researchsquare.com)
However, one of the bottlenecks in the stem cell therapy is that stem cells tend to differentiate into other types of cells during culture in vitro. (biomedcentral.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
Next, we showed that the resulting hybrids can differentiate and acquire a proto-neural electrophysiology profile when the Wnt/beta-catenin pathway is activated in the adult stem cells prior fusion. (regenerativemedicine.net)
Interpretation: We show fusion between human MG and adult stem cells, and demonstrate that the resulting hybrid cells can differentiate towards neural fate in human model systems. (regenerativemedicine.net)

Endothelial cell proliferation is a common feature of prolife. (shengsci.com)
In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. (tno.nl)
The culture system, in which a marrow-derived adherent cell population, established in vitro, exerts a long-term promoting influence on proliferation of haemopoietic cells, is reproduced. (tno.nl)
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. (tno.nl)
In this study, our aim was to evaluate the proliferation pattern as well as the resistance to spontaneous transformation of hASCs during expansion in a xeno-free culture condition. (biomedcentral.com)
This complete characterization of hASCs cultivated in pooled allogeneic human serum, a suitable xeno-free approach, shows that pooled allogeneic human serum provides a high proliferation rate, which can be attributed for the first time to C-MYC protein expression, and showed cell stability for safe clinical applications in compliance with good manufacturing practice. (biomedcentral.com)
For culturing of adherent cells, a range is limited within a scaffold in which the cells grow during the course of their division and proliferation, and therefore with continued proliferation it is unavoidable that they eventually reach the limit of proliferation in the scaffold. (justia.com)
Knockdown of TET1 or ectopic expression of TET2 in T-ALL was associated with genome-wide changes in 5mC and 5hmC enrichment and decreased cell proliferation, suggesting a tumor promoting function of TET1, and a tumor suppressing role for TET2. (biomedcentral.com)
We found that the depletion of Nanog inhibited proliferation and promoted apoptosis in EpCAM + CD44 + HCT-116 cells. (researchsquare.com)
One compelling idea to explain the Warburg effect is that the altered metabolism of cancer cells confers a selective advantage for survival and proliferation in the unique tumor microenvironment. (pharmaceuticalintelligence.com)

They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
Proliferating endothelial cell-specific expression of IGF-I receptor ribozyme inhibits retinal neovascularization. (shengsci.com)
Insulin-like growth factor-I (IGF-I) and its receptor (IGF-IR) are essential for normal ocular development and are expressed in numerous ocular cell types including lens epithelial cells, retinal pigment epithelial cells, Müller cells and endothelial cells. (shengsci.com)
Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
Hemogenic endothelial cells (HECs) are specialized cells that undergo endothelial-to-hematopoietic transition (EHT) to give rise to the earliest precursors of hematopoietic progenitors that will eventually sustain hematopoiesis throughout the lifetime of an organism. (bu.edu)
Explant cultures of murine and human fetal lungs display adherent endothelial cells transitioning into floating hematopoietic cells, accompanied by the gradual loss of an endothelial signature. (bu.edu)
Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. (thermofisher.com)
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. (thermofisher.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)

To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)

CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
The Center for Regenerative Medicine (CReM) is a joint effort between Boston University and Boston Medical Center that brings together nine principal investigators addressing various aspects of developmental biology, stem cells, regeneration and injury, cell lineage specification and disease modeling with a major focus on induced Pluripotent Stem Cells or iPSCs. (bu.edu)

These bone marrow stem cells do not contribute to the formation of blood cells, and so do not express the hematopoietic stem cell marker CD34. (wikipedia.org)

More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
Neural stem cells have long lives, much of which they spend in a quiescent state. (elifesciences.org)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
The neural stem cells, which Mich, Signer et al. (elifesciences.org)

In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. (tno.nl)
Preferred Cell Systems™ is the exclusive manufacturer and supplier of all reagents and in vitro assays originally produced by HemoGenix. (preferred-cell-systems.com)
More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)

In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
Prior to their clinical application, hASCs must be expanded ex vivo to obtain the required number of cells for transplantation. (biomedcentral.com)
2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
In recent years, with increasing interest being directed toward cultured cell-based production of vaccines, and in vivo proteins such as enzymes, hormones, antibodies and cytokines, as well as cell grafts for use in regenerative medicine, greater focus has been placed on efficient and convenient methodologies for mass cell culturing. (justia.com)
Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
However, in mice, in vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons. (regenerativemedicine.net)
In this study, the finding that a significant proportion of all dendritic cells (DCs) resident in vivo in the human postnatal thymus displayed a myeloid-related phenotype prompted us to re-examine the developmental origin of thymic DCs, a cell type hitherto considered to represent a homogeneous lymphoid-derived population. (ashpublications.org)
We also demonstrate that phenotypically and functionally equivalent myeloid precursors devoid of T-cell potential do exist in vivo in the postnatal thymus. (ashpublications.org)
8 As these latter DCs derive from an intrathymic lymphoid-restricted progenitor able to generate also T, B, and NK cells, but not myeloid cells, upon transfer in vivo, 5 , 9 , 10 they have hitherto been considered of lymphoid origin. (ashpublications.org)

Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma, or the dental pulp of deciduous (baby) teeth. (wikipedia.org)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
A focus is on the steps of mobilization of cells from the subchondral bone and repair tissue formation, including adherence of the bone marrow clot to the subchondral bone. (hindawi.com)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Worldwide, 50,000 hematopoietic stem cell transplant (HSCT) procedures are undertaken annually to replace a person's blood-forming cells - hematopoietic stem cells (HSCs) - to treat blood disorders and cancers, yet two-thirds of patients who need HSCT lack matched donor tissue. (utoronto.ca)
Human adipose tissue-derived stem cells (hASCs) are attractive cells for therapeutic applications and are currently being evaluated in multiple clinical trials. (biomedcentral.com)
and (2) through the paracrine release of trophic factors to induce tissue repair by endogenous cells [ 6 ]. (biomedcentral.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
1. Activation of adipose tissue and related mesenchymal stem cells: promotion of anti-inflammatory properties through SOX2 transcription factor and HuR RNA-binding protein modulation. (neurogelenmarche-usa.org)
1999). Recent studies have shown that the potentiality of tissue regeneration can be enhanced using adipose derived stem cells (hADSCs) (see Gimble et al. (neurogelenmarche-usa.org)
2011). Stem cell frequency appears to be significantly higher in adipose tissue than in other tissues such as bone marrow (Bieback et al. (neurogelenmarche-usa.org)
We have recently reported that mechanical manipulation of human adipose tissue without modification of tissue composition, structure and histology, that was totally comparable to that of the original source (see Experimental procedure for details), increased significantly the number of stromal cells bearing the expression of beta-tubulin III. (neurogelenmarche-usa.org)
We have suspected that mechanical forces may be of key relevance in adult tissue plasticity as it occurs during embryogenesis, tissue remodeling and mitotic and motile behavior of cells (Vogel et al, 2006). (neurogelenmarche-usa.org)
The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
A proteome can be defined as the complete set of proteins expressed in a particular cell or tissue of interest. (stanford.edu)

While they are described as multipotent, it is possible that they are pluripotent. (wikipedia.org)
With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)

In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)

We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)

Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
Recently, emerging evidences have demonstrated that Nanog is expressed in a variety of cancer cell lines and tissues, and is associated with aggressive tumors [10, 11]. (researchsquare.com)

2006). Furthermore, several recently published reports claim that hADSCs are capable under appropriate stimulation of differentiating into other different cell types such as neurons, cardiomyocytes, hepatocytes and pancreatic cells (Schäffler et al. (neurogelenmarche-usa.org)
Promotes transdifferentiation of hepatocytes from mouse pancreatic cells (Shen et al. (stemcell.com)

Cost-effective blood cell generation will require novel strategies based on better knowledge of underlying mechanisms driving in vitro development. (biomedcentral.com)

1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
7 There has been some variation and question regarding the correct terminology for this population of stromal adipose cells. (journalofprolotherapy.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
Tumor and lymphoid compartments sparsely expressed immunosuppressive targets commonly investigated in clinical trials, such as the programmed cell death protein-1/programmed death ligand-1 axis. (bu.edu)
Stem cells have been extensively explored for a variety of regenerative medical applications and they play an important role in clinical treatment of many diseases. (biomedcentral.com)

This team, which brings together expertise in stem cell biology, cell biology, developmental signalling pathways, and genetic screens, aims at improving methods to achieve generation of HSC. (utoronto.ca)

A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. (tno.nl)
Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. (tno.nl)

NIHMS1512949-health supplement-4.jpg (110K) GUID:?06D54B37-Compact disc61-49FB-982B-7EFB00148FD4 Abstract Calcitriol, the active type of vitamin D, continues to be well documented to do something in immune system cells and malignant cells straight. (synanet2020.com)

Methods: We used organotypic cultures of human retina and preparations of dissociated cells to test the hypothesis that cell fusion between human MG and adult stem cells can induce neuronal regeneration in human systems. (regenerativemedicine.net)
Findings: We first found that cell fusion occurs between MG and adult stem cells, in organotypic cultures of human retina as well as in cell cultures. (regenerativemedicine.net)

term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
The group plans to identify better markers to track and purify key cell intermediates as well as uncover key genes/pathways that lead to HSC development. (utoronto.ca)

Fetal bovine serum is the supplement most widely used for cell culture, but it has disadvantages and it is not safe for cell therapy due to the risks of pathogen transmission and immune reaction. (biomedcentral.com)
however, C-MYC protein expression was enhanced in pooled allogeneic human serum cultures compared to fetal bovine serum cultures. (biomedcentral.com)

Despite advances in using umbilical cord blood, donor material remains restricted by limited stem cells and the lack of ethnic diversity to provide sufficiently matched material. (utoronto.ca)

Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)

Therefore, when an excessive amount of medium has been used, it is often the case that oxygen may be insufficiently supplied to the cells and the low oxygen condition may lead to inhibition or even cell death. (justia.com)
These inhibitors are selectively inhibitors of nuclear move (SINE), and possess been demonstrated to end up being effective for dealing with specific types of malignancies, including pancreatic tumor, severe myeloid leukemia, mantle cell lymphoma, causing in significant development apoptosis and inhibition of tumour cells with no serious toxicity [20]C[22]. (cancerhugs.com)

most of the neutrophils in the body are contained in the bone marrow, either as mitotically active (one third) or postmitotic mature cells (two thirds). (medscape.com)
A common reference range for the absolute neutrophil count (ANC), which is calculated on the basis of the percentage of neutrophils in the white blood cell count (see the Absolute Neutrophil Count calculator) is 2500-8000/µL. (medscape.com)
During the recovery phase, the flow of cells from the marrow decreases, with a resultant decrease in the number of neutrophils. (medscape.com)

Recent studies indicate that stem cell-based regenerative therapies can restore severe damaged skin both structurally and functionally. (bvsalud.org)
The methods proposed are cutting-edge and no other team in the world has this combination of technologies, approaches and expertise to make advances in hematopoietic cell therapies. (utoronto.ca)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)

Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
As a solution for the issue of oxygen supply, it has been attempted to increase the oxygen concentration by utilizing microbubbles (PTL 1) or to employ methods for uniformly supplying oxygen in microcarrier culturing (PTL 2). (justia.com)
Methods of culturing while exposing the cell culture support to a gas phase have also been attempted. (justia.com)
Methods: To investigate whether Nanog plays a crucial role in maintaining the stemness of colorectal CSCs (CCSCs), RNA interference was used to downregulate Nanog expression in the CRC stem cell line, EpCAM + CD44 + HCT-116. (researchsquare.com)

Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

Colony formation assay, sphere-forming ability assay, alkaline phosphatase (AP) activity assay and teratoma-formation assay were used to assess the role of modaline sulfate (MDLS) in promoting self-renewal and reinforcing pluripotency of P19 cells. (biomedcentral.com)

Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)

A skin substitute was developed by mixing hNSCs-derived skin cells (hNSCs-SCs) in plasma for transplantation in a rat model of severe burn injury. (bvsalud.org)
Source material for hematopoietic (bone marrow) transplantation is in great demand as at least 20,000 allogeneic transplants are performed each year. (utoronto.ca)

They are sometimes referred to as bone marrow stromal stem cells. (wikipedia.org)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. (tno.nl)
The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
This is followed rapidly by egress of cells from the marrow, resulting in an increase in the TBGP and blood neutrophilia. (medscape.com)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)

The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)

The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. (heraldopenaccess.us)

Our group and others have previously shown the potential of this approach for the dominantly inherited neurological disease DYT1 dystonia by achieving potent short-hairpin RNA (shRNA)-mediated silencing of the disease protein, torsinA, in cultured cells. (shengsci.com)
An invasive Escherichia coli expressing the inv gene from Yersinia pseudotuberculosis was used as a vector for protein delivery to mammalian epithelial cells. (shengsci.com)
Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
During human being carcinogenesis or malignancy development, cancerous cells acquire the capability to move important nuclear protein that can impact treatment efficiency. (cancerhugs.com)
These protein consist of growth government bodies and suppressors of cell apoptosis, nuclear localization of which can be needed for their correct function [4]. (cancerhugs.com)
CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. (thermofisher.com)

Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
Our results suggest that cell fusion-mediated therapy is a potential regenerative approach for treating human retinal dystrophies. (regenerativemedicine.net)

Flow cytometry was used to measure the uptake of adriamycin (AM) and daunomycin (DM) by various cell types of the haemopoietic organs and by acute myeloid leukemia (AML) cells. (tno.nl)
However, infiltrating myeloid cell types within both primary and metastatic GEP-NETs were enriched for genes encoding other immune checkpoints, including VSIR (VISTA), HAVCR2 (TIM3), LGALS9 (Gal-9), and SIGLEC10. (bu.edu)
Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
1-3 These nonoverlapping functions have been proposed to result from the actions of 2 major DC populations which have been characterized as myeloid and lymphoid DCs, respectively, on the basis of their anatomical localization and cell-surface phenotypes and, ultimately, of their distinct developmental origin. (ashpublications.org)

Development of viable autologous cell therapy for treatment of heart failure in the elderly requires the need to address MSC ageing. (bvsalud.org)
KPT-185 and KPT-276) in NSCLC cells and to ideally offer book understanding into these medicines for potential focus SCH 442416 IC50 on therapy of NSCLC. (cancerhugs.com)

CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. (thermofisher.com)
Advantages (C-E). The altered metabolism of cancer cells is likely to imbue them with several proliferative and survival advantages, such as enabling cancer cells to execute the biosynthesis of macromolecules (C), to avoid apoptosis (D), and to engage in local metabolite-based paracrine and autocrine signaling (E). Potential Liabilities (F and G). This altered metabolism, however, may also confer several vulnerabilities on cancer cells. (pharmaceuticalintelligence.com)

Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)
Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). (thermofisher.com)

Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. (thermofisher.com)

Here, we investigated the single-cell transcriptomes of tumor and immune cells from patients with gastroenteropancreatic NETs. (bu.edu)
While aberrant DNA methylation is a characteristic feature of tumor cells, our knowledge of how these DNA methylation patterns are established and maintained is limited. (biomedcentral.com)
Here, we report that in T cell acute lymphoblastic leukemia (T-ALL) the MYC oncogene controls the expression of TET1 and TET2 to maintain 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) patterns, which is associated with tumor cell-specific gene expression. (biomedcentral.com)
For use with primary tumor cells and transformed cell lines. (preferred-cell-systems.com)
Study the interaction of tumor cells with other normal cell types, e.g. immune cells, mesenchymal stem/progenitor cells. (preferred-cell-systems.com)
Study the effect of agents on tumor cell growth. (preferred-cell-systems.com)
Study tumor stem cells. (preferred-cell-systems.com)
CellaGlo™ can be used for tumor cells from multiple species. (preferred-cell-systems.com)
Different tumor cells and cell lines have different growth requirements. (preferred-cell-systems.com)
Conclusions: In conclusion, these findings collectively demonstrate that Nanog, which is highly expressed in CRC stem cells, is a key factor in the development of tumor growth, and it may serve as a potential marker of prognosis and a novel and effective therapeutic target for the treatment of CRC. (researchsquare.com)
Results The current research proven the anti-tumor results of KPT-185 in NSCLC cells, including EGFR-TKI-resistant NSCLC cell lines. (cancerhugs.com)
Drivers (A and B). The metabolic derangements in cancer cells may arise either from the selection of cells that have adapted to the tumor microenvironment or from aberrant signaling due to oncogene activation. (pharmaceuticalintelligence.com)

Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. (tno.nl)
Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)

Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)

If the demand of cells is high, a shift to the left in the differential count may occur. (medscape.com)

These findings demonstrated, for the first time, that MDLS could maintain self-renewal and pluripotency of stem cells. (biomedcentral.com)

A cell can be classified as an MSC if it shows plastic adherent properties under normal culture conditions and has a fibroblast-like morphology. (wikipedia.org)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)

Thus, it is vital to improve the current therapeutic strategies for CRC and find novel treatments to eradicate cancer stem cells. (researchsquare.com)
HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)

Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)

These stem cells are capable of differentiating into chondrocytes, cardiomyocytes, melanocytes, and hepatocyte‐like cells in vitro. (wikipedia.org)

Anatomy51

Organisms15

Diseases16

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Psychiatry and Psychology1

Phenomena and Processes43

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Named Groups2

Health Care2

Embryonic13

MSCs39

Differentiation21

Differentiate9

Proliferation10

Endothelial11

Progenitor cell1

IPSCs2

CD341

Neural Stem8

Assays3

Vivo10

Tissue30

Multipotent2

Stromal cell3

CD451

Tissues2

Pancreatic cells2

Driving in vitro1

Adipose3

Clinical8

Biology1

Mouse bone marrow2

Abstract1

Adult stem2

Markers2

Fetal2

Umbilical cord1

Antigen-prese2

Inhibition2

Neutrophils3

Therapies4

Methods4

Undergo1

Assay1

Osteoblasts1

Transplantation2

Marrow8

Mesenchymal stromal2

Monolayer cell cultures2

Protein6

Regenerative2

Myeloid4

Therapy2

Apoptosis2

Receptor2

Dendritic2

Tumor12

Formation2

Autologous1

Differential1

Self-renewal and pluripotency1

Morphology1

Immune5

Therapeutic2

Microenvironment1

Chondrocytes1