Pluripotent cell differentiation. Medical search. Frequent questions

Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. (bioregate.com)

Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. (edu.au)
Applying Expedition to single pluripotent stem cells undergoing neuronal differentiation, we discover that up to 20% of AS exons exhibit bimodality. (nih.gov)
In this study, we used iPSC-derived neural progenitor cells (NPCs) from individuals with ASD of known and unknown genetic cause as well as from neurotypical controls to examine the effects of exogenous IL-17a on NPC proliferation, migration and neuronal differentiation, and whether IL-17a and genetic risk factors for ASD interact exacerbating alterations in NPC function. (figshare.com)
In conclusion, our results suggest that exogenous IL-17a positively regulates the neuronal differentiation of human NPCs, which may disturb normal neuronal and synaptic development and contribute to MIA-related changes in brain function and behavior. (figshare.com)

To determine mechanisms in these stage-specific lineage interactions, we modeled potential effects of soluble signals derived from immortalized human fetal liver parenchymal cells on stem cells, including embryonic and induced pluripotent stem cells. (elsevierpure.com)
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Lactate may be applied to produce hepatocytes from iPS cells more efficiently. (spandidos-publications.com)
Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
These methods have been previously used to obtain hepatocytes from iPS cells. (spandidos-publications.com)
Therefore, methods for obtaining hepatocytes from iPS cells should be further investigated. (spandidos-publications.com)
A Hepatocyte selection medium (HSM) was developed to enrich hepatocytes from co-cultures with iPS cells ( 18 ). (spandidos-publications.com)
Gene expression profiling and comparison of regulatory networks in immature and mature hepatocytes revealed stem cell-derived hepatocytes represented early fetal-like stage. (elsevierpure.com)
The extent of metabolic and synthetic functions in stem cell-derived hepatocytes was sufficient for providing hepatic support along with promotion of tissue repair to rescue mice in acute liver failure. (elsevierpure.com)
They are polarized cells that breathe aerobically, consume lactate, and contain as many mitochondria as the human hepatocytes. (wikipedia.org)
One unique characteristic of HepaRG cells is that they can survive up to four weeks in culture, making them useful for long-term studies and repeated exposures to drugs and chemicals, unlike primary human hepatocytes. (wikipedia.org)
In contrast to many other cell lines, HepaRG cells are capable of regulating glycogenolysis and gluconeogenesis at levels similar to primary human hepatocytes, as well as retaining the response mechanisms associated with lipid-metabolizing enzymes. (wikipedia.org)
In this study, we examined whether human iPS cells, cultured on scaffolds, can differentiate into definitive endodermal cells, as precursor for hepatocytes, pancreatic and lung cells. (ac.ir)
Advanced techniques and awaited clinical applications for human pluripotent stem cell differentiation into hepatocytes. (amedeo.com)

Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
Here we report the differentiation of human colonic organoids (HCOs) from hPSCs. (bioregate.com)
Recent breakthroughs in human pluripotent stem cell-derived cerebral organoids provide a valuable platform for investigating the human brain after different drugs treatments and for understanding the complex genetic background to human pathology. (frontiersin.org)
Here, we identified tranylcypromine, which is used to treat refractory depression, caused human-induced pluripotent stem cell-derived brain organoids neurotoxicity, leading to decreased proliferation activity and apoptosis induction. (frontiersin.org)
Recent breakthroughs in human pluripotent stem cells (hPSCs)-derived cerebral organoids offer a promising approach for investigating the mystery of human brain ( 19 ). (frontiersin.org)
Our investigations utilize in vivo mouse retina and human retinal organoids derived from pluripotent stem cells as study systems. (nih.gov)
Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)

Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. (unlp.edu.ar)
To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
Human pluripotent stem cells, including ESCs and iPSCs, are highly sensitive cells and undergo apoptosis during these routine procedures. (nih.gov)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)

abstract = "Adoptive cell transfer (ACT) of antigen-specific CD8+ cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1. (psu.edu)
Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)

Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
Induced pluripotent stem (iPS) cells have shown potential for differentiation and may become a resource of functional neurons for the treatment of PD. (globalauthorid.com)
This study examined whether functional neurons could be efficiently generated from iPS cells using a five-step induction procedure combined with docosahexaenoic acid (DHA) treatment. (globalauthorid.com)
DHA treatment facilitated iPS differentiation into tyrosine hydroxylase (TH)-positive neurons in vitro and in vivo and functionally increased dopamine release in transplanted grafts in PD-like animals. (globalauthorid.com)
Therefore, our data suggest that DHA plays a crucial role in iPS differentiation into functional DA neurons and that this approach could provide a novel therapeutic approach for PD treatment. (globalauthorid.com)
Moreover, tranylcypromine treatment affects neurons and astrocytes, which impairs cell density and arrangement. (frontiersin.org)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
The goal of our research is to develop stem cell-based therapies to replace lost neurons as a treatment for brain diseases. (lu.se)
With this knowledge at hand, we become better at controlling the differentiation of human pluripotent cells to subtype-specific neurons which can be used for disease modeling, drug screening, and transplantation, leading to new treatments for several types of brain disorders. (lu.se)
We aim to develop novel, lasting therapies for neurodegenerative diseases through the replacement of lost neurons in the brain with new neurons generated from stem cells. (lu.se)

We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
My lab integrates traditional and single-nuclei RNA sequencing to elucidate transcriptome-wide changes to RNA metabolism in clinically relevant post-mortem brain tissue and human induced pluripotent stem cell (hiPSC) models of neurological disease including ALS and autism. (ed.ac.uk)

In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)

We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
The implementation of human pluripotent stem cells (hiPSCs) in cell therapy has an extraordinary potential but faces many practical challenges, including costs associated with growth media and factors. (authorea.com)
There is an immediate need to establish an optimized culture platform to direct the differentiation of hiPSCs into germ layers in a defined nutritional microenvironment to generate cost-effective and robust therapeutics. (authorea.com)
The aim of this study was to identify the optimal nutritional environment by mimicking the in vivo concentrations of three key factors (glucose, pyruvate and oxygen) during the spontaneous differentiation of hiPSCs derived from cord blood, which greatly differ from the in vitro expansion and differentiation scenarios. (authorea.com)
A design of experiments (DoE) was used to investigate the interaction between these three variables during the spontaneous differentiation of hiPSCs. (authorea.com)
Our findings present a novel approach for efficient directed differentiation of hiPSCs through the manipulation of media components whilst simultaneously avoiding the usage of growth factors thus reducing costs. (authorea.com)
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. (mdc-berlin.de)
However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. (mdc-berlin.de)
We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. (mdc-berlin.de)
We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. (mdc-berlin.de)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)

Our focus on mesoderm leads us on to molecular pathways for early human cardiomyocyte differentiation, with a goal of understanding the transcriptional networks responsible for cardiomyocyte identity and using this to generate more homogeneous cardiomyocyte populations for therapeutic applications and drug discovery. (qscience.com)
The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. (musc.edu)

All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)

The group led by Dr. Juan Carlos del Pozo has found that the transcription factor OBP4 activates cell proliferation in differentiated pericycle cells. (upm.es)
OBP4 does not induce cell division in the root meristem but it promotes pericycle cell proliferation, forming callus-likes structures at the root tip as shown by expression of stem cells markers. (upm.es)
Exogenous IL-17a did not induce abnormal proliferation and migration of ASD and control NPCs but, on the other hand, it significantly increased the expression of synaptic (Synaptophysin-1, Synapsin-1) and neuronal polarity (MAP2) proteins in these cells. (figshare.com)
Angiotensin-converting enzyme (ACE) stimulates hematopoietic precursors proliferation and erythroid differentiation. (bvsalud.org)
BACKGROUND: Glioblastoma is the most aggressive form of brain cancer, characterised by high proliferation rates and cell invasiveness. (bvsalud.org)
For this purpose, three glioblastoma cell lines were tested, and the QMR effect was evaluated on cancer cell proliferation rate and aggressiveness. (bvsalud.org)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
A complex process that involves the proliferation of MEGAKARYOCYTES in BONE MARROW, their differentiation in size and ploidy, and maturation before the release of functional BLOOD PLATELETS. (bvsalud.org)

However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
For identifying lineage conversion and maturation, we utilized conventional assays of cell morphology, gene expression analysis and lineage markers. (elsevierpure.com)
Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)

Many research groups are engaged in using human pluripotent stem cells (hPSCs) to generate surrogate pancreatic beta-cells for transplantation into diabetic patients. (ca.gov)
However, to our knowledge, there is no report on the successful generation of glucose-responsive insulin-producing beta-cells from hPSCs in vitro. (ca.gov)
Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
Accordingly, human pluripotent stem cells (hPSCs) with the properties of self-renewal and pluripotency present an ideal cell model for the isolation of pericytes to study their development or the therapeutic effect in pericyte-related diseases. (researchsquare.com)
Here, we successfully derive pericyte-like cells with cranial neural crest (CNC) origin from hPSCs (designated as hPSC-CNC PCs), which may represent an ideal cell source for the treatment of pericyte dysfunction-related disorders and help to model the human BBB in vitro for the study of the pathogenesis of such neurological diseases. (researchsquare.com)
Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)

Metabolic properties were analyzed to determine the extent of hepatic differentiation. (elsevierpure.com)
Cell transplantation studies were performed in mice with drug-induced acute liver failure to elicit benefits in hepatic support and tissue regeneration. (elsevierpure.com)
These studies showed signals emanating from fetal liver cells induced hepatic differentiation in stem cells. (elsevierpure.com)
We concluded that hepatic differentiation of pluripotent stem cells with metabolomics products will be significant for developing therapies. (elsevierpure.com)
Bandi, S , Tchaikovskaya, T & Gupta, S 2019, ' Hepatic differentiation of human pluripotent stem cells by developmental stage-related metabolomics products ', Differentiation , vol. 105, pp. 54-70. (elsevierpure.com)
HepaRG cell line is a human hepatic in vitro line used in liver biology research and for assessing liver pathology, hepatotoxicity, and drug-induced injury. (wikipedia.org)
In contrast to the historic hepatic line HepG2, HepaRG cells preserved various liver-specific functions, including the expression of CYP enzymes and transporters, the formation of bile canaliculi, and, the ability to be applied in 2D and 3D configuration. (wikipedia.org)
Rumin observed a group of cells that resembled hepatic cells and gradually lost HCV infection markers. (wikipedia.org)
The HepaRG cells have been found to express major nuclear receptors, as well as drug and bile acids transporters, and key hepatic nuclear factors. (wikipedia.org)
See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)

In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)

In recent studies we have examined the role of transforming growth factor family members in both pluripotency and differentiation. (qscience.com)
EpiSCs share many features with hESCs, and subsequent work supports our hypothesis that hESCs are the human counterparts of EpiSCs, with similar responses to growth factors and mechanisms of pluripotency and differentiation. (qscience.com)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
In stem cells exposed to signals from fetal cells, mechanistic gene networks of upstream regulators decreased pluripotency, while simultaneously inducing mesenchymal and epithelial properties. (elsevierpure.com)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
HepaRG cells are considered fit-for-all-purpose cells as they were used in many applications ranging from drug development, drug metabolism and interaction assessment, chemical testing, assay validation, hepatotoxicity assessments, liver Biology, liver disease characterization, and virology studies. (wikipedia.org)
Systematic AS characterization in single cells redefines our understanding of AS complexity in cell biology. (nih.gov)
This invention will significantly improve quality assurance, characterization, and application of pluripotent stem cells in both pre-clinical and clinical settings. (nih.gov)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
It was hypothesized that the addition of glucose and galactose promoted hepatocyte differentiation of iPS cell. (spandidos-publications.com)
The HSM was modified into a hepatocyte differentiation inducer (HDI). (spandidos-publications.com)
Philippe Gripon later developed these cells, finding that they had the ability to undergo complete hepatocyte differentiation while retaining all liver-specific functions. (wikipedia.org)

Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage. (ca.gov)
Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
This work reveals the importance of BRACHYURY and CDX2 genes as key mediators of embryonic and extraembryonic lineage differentiation in hESCs and EpiSCs. (qscience.com)
During liver development, cytokines released from various cell types are critical for stem/progenitor cell differentiation and lineage expansions. (elsevierpure.com)
or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

The cells were differentiated into definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)
In the present study, cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively.The results of this study demonstrated the positive effect of 3D cultures, by using suitable factors, on definitive endoderm differentiation.definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)

In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
We are now focused on delineating the transcription factors and signaling pathways that are responsible for generating photoreceptors from retinal progenitor cells. (nih.gov)
Can we find common cellular pathways associated with photoreceptor cell death caused by distinct genetic mutations? (nih.gov)

In our most recent work we have focused on the role of bone morphogenetic protein (BMP)-4 in the cell fate decision between endoderm and mesoderm, demonstrating the similarity of BMP-induced hESC and EpiSC differentiation to mesoderm induction during mouse gastrulation. (qscience.com)

Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. (unlp.edu.ar)
In the present work, the Quantum Molecular Resonance® (QMR) technology was used to create a regular microchannel structure inside the connective tissue of full-thickness decellularized tubular porcine esophagi to facilitate a diffuse and uniform spreading of seeded mesenchymal stromal cells within the scaffold. (bvsalud.org)
The scaffold was seeded with mesenchymal stromal cells under dynamic conditions, to assess the ability to be repopulated before its implantation in a large animal model. (bvsalud.org)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)

In a single experiment, cells that shared a progenitor were more transcriptionally similar to each other than to other cells in the differentiated population. (figshare.com)
The differentiation mechanisms involving metabolomics products could have an impact on advancing recruitment of stem/progenitor cells during tissue homeostasis. (elsevierpure.com)
To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
This project seeks to elucidate transcriptional and post-transcriptional regulatory networks that determine cell fate and guide the development of photoreceptors from retinal progenitor cells. (nih.gov)

Additionally, we found that substantial cell death occurs during differentiation in a manner that suggested all cells were equally likely to survive or die, suggesting that there is no intrinsic selection bias for cells descended from particular hiPS cell progenitors. (figshare.com)
We show that Zeb2 over-expression has a positive impact on skeletal muscle differentiation in pluripotent stem cells and adult myogenic progenitors. (unipv.it)
HepaRG cells are bipotent progenitors, capable of differentiating into both biliary and hepatocyte lineages. (wikipedia.org)
What are intrinsic control mechanisms that lead to photoreceptor cell fate from retinal progenitors? (nih.gov)

Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)

However, the scarcity of material sources greatly limits the application of primary pericytes in disease modeling and cell transplantation studies. (researchsquare.com)

Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
These transcription factors bind promoters and distant regulatory regions, including E-box elements, of genes whose expression is restricted to muscle cells. (unipv.it)
Furthermore, DHA dramatically upregulated the endogenous expression levels of neuroprotective genes ( Bcl-2, Bcl-xl, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor) and protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis in iPS-derived neuronal precursor cells. (globalauthorid.com)
Furthermore, we found that DHA treatment in iPS cell-grafted rats significantly downregulated the mRNA expression of embryonic stem cell-specific genes (Oct-4 and c-Myc) in the graft and effectively blocked teratoma formation. (globalauthorid.com)
The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)

In the Kirkeby lab, we further apply advanced human stem cells models and single-cell RNA sequencing to understand how the hundreds of human neural subtypes of cells are formed during embryo development. (lu.se)

It is known that less-differentiated and central-memory-like (termed highly reactive) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7 . (psu.edu)

Our research focuses on understanding how pluripotent mammalian stem cells maintain their undifferentiated state and undergo differentiation in culture - this reflecting my enduring interest in the emergence of diversity during mouse gastrulation. (qscience.com)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)
This activation promotes the formation of callus, a mass of proliferative cells in an undifferentiated state. (upm.es)
many of them involved in hormonal signaling and in the formation of callus, a mass of proliferative cells derived from reprogrammed pericycle cells. (upm.es)

However, when the same hiPS cells were differentiated in parallel, we did not observe high transcriptional similarity across differentiations. (figshare.com)

Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)

Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
The process of generating thrombocytes (BLOOD PLATELETS) from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via the MEGAKARYOCYTES. (bvsalud.org)

However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. (psu.edu)
Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs. (psu.edu)
According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)

Some plant cells types, such as pericycle cells, have a remarkable plasticity to be reprogrammed and to regenerate new organs and functional plants. (upm.es)
In this work, a functional screening of transcription factors, using the Spanish collection TRANSPLANTA, identifies Arabidopsis OBP4 as a novel regulator of root growth by reducing cell elongation and cell differentiation. (upm.es)
Our data provide molecular insights into how differentiated root cells acquire the potential to generate callus, a pluripotent mass of cells that can regenerate fully functional organs and plants. (upm.es)
Additionally, the HepaRG cells have functional mitochondria, hepatokine secretion abilities, and a suitable response to insulin. (wikipedia.org)
A study demonstrated that HepaRG cells can be incorporated into a microfluidic system to form a functional bio-artificial liver capable of detoxifying ammonia and other harmful substances. (wikipedia.org)
Importantly, the in vivo experiment suggests that DHA induces the differentiation of functional dopaminergic precursors and improves the abnormal behavior of 6-OHDA-treated PD-like rats by 4 months after transplantation. (globalauthorid.com)
Protein phosphatase 2A regulation of markers of extracellular matrix remodelling in hepatocellular carcinoma cells: functional consequences for tumour invasion. (tcd.ie)
We previously discovered that that Maf-family bZIP transcription factor NRL is critical for rod photoreceptor fate and functional differentiation, and that loss of NRL leads to S-cones instead of rods. (nih.gov)

Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. (unlp.edu.ar)
By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
However, the molecular mechanisms underlying cell reprogramming are not completely known. (upm.es)
The answers to these questions will be valuable for delineating pathogenic mechanisms that contribute to photoreceptor cell death. (nih.gov)

Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)

or as fully differentiated cells that are ready and easy-to-use cells with high inter-assay reproducibility and proven functionality across multiple applications. (wikipedia.org)
Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
METHODS: We assessed the clonogenic potential by colony-forming unit (CFU) assay of mononuclear cells isolated from PV JAK2 positive or JAK2 negative patients with erythrocytosis treated with enalaprilat or losartan. (bvsalud.org)

Differentiation of pluripotent embryonic stem cells into cardiomyocytes. (bvsalud.org)

In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)

In vitro, HCOs express colonic markers and contained colon-specific cell populations. (bioregate.com)
To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)

However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
In particular, we aim to enter a clinical trial with a pluripotent cell product for the treatment of Parkinson's Disease, and are also developing preclinical cell therapies for narcolepsy and dementia. (lu.se)
To develop novel cell therapies for neurodegenerative diseases. (lu.se)
Such stem cell therapies have the potential to repair the damaged brain, making patients independent of daily medications with severe side effects. (lu.se)
Through our work on developing novel stem cell therapies for brain diseases, we contribute to the main goal of MultiPark on establishing novel therapeutic approaches for developing more effective symptomatic, new disease-modifying, and/or plasticity-enhancing treatments. (lu.se)

RESULTS: Treatment with drugs led to a decrease of erythroid precursor frequency both in the presence and absence of JAK2 mutation, with a high extent in JAK2 positive cells and without affecting other types of precursors. (bvsalud.org)

A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)

Taken together, these studies should significantly accelerate the progression from basic stem cell research to clinical applications. (qscience.com)
Tissue engineering techniques for the creation of customized "self-developing" esophageal substitutes, which are obtained by seeding autologous cells on artificial or natural scaffolds, allow simplifying surgical procedures and achieving good clinical outcomes. (bvsalud.org)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
To enter a clinical trial with a stem cell product for the treatment of Parkinson's Disease. (lu.se)

These studies led to our discovery of a novel type of pluripotent epiblast stem cell (EpiSC) from the late epiblast layer of mouse and rat embryos. (qscience.com)
During the transition from the naive epiblast to a pluripotent state, over 100 cells are organized into an approximately 60-80 µm diameter pre-implantation mouse embryo. (zeiss.com)

Retinal ganglion cells can be purified and matured in vitro to generate models for optic neuropathies. (indianactsi.org)

Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
Recent iPS cell technology and the development of an in vitro system for gene delivery are capable of generating iPS cells from patients without any surgical approach. (psu.edu)
Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
Bimodal exons are flanked by more conserved intronic sequences harboring distinct cis-regulatory motifs, constitute much of cell-type-specific splicing, are highly dynamic during cellular transitions, preserve reading frame, and reveal intricacy of cell states invisible to conventional gene expression analysis. (nih.gov)
We demonstrated that DHA, a ligand for the RXR/Nurr1 heterodimer, significantly activated expression of the Nurr1 gene and the Nurr1-related pathway in iPS cells. (globalauthorid.com)
Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
We are using state-of-the-art next generation sequencing combined with bioinformatic strategies, and developing stem cell-based approaches for gene therapy and drug discovery. (nih.gov)

Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

Endogenous cell signals regulate tissue homeostasis and are significant for directing the fate of stem cells. (elsevierpure.com)
In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)

Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
We then carried out a detailed analysis of the roles of Smad proteins (Smad2 and Smad3) as direct regulators of Nanog, which in turn blocks an hESC default differentiation into neuroectoderm. (qscience.com)
b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
Unexpectedly, differentiation-inducing soluble signals constituted metabolomics products and not proteins. (elsevierpure.com)
Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)

Anatomy62

Organisms11

Diseases6

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes62

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Differentiated from human pluripotent stem1

Neuronal differentiation4

Embryonic and induced4

Hepatocytes12

Organoids8

IPSCs15

Abstract2

Neurons13

Human induced plur2

Hematopoietic cells1

HiPSCs11

Cardiomyocyte differentiation2

Growth and differentiation2

Proliferation8

Maturation5

HPSCs9

Hepatic10

Vivo differentiation1

Pluripotency7

Characterization5

Hepatocyte differentiation5

Lineage6

Definitive endoderm2

Pathways3

Endoderm1

Mesenchymal4

Cardiac1

Progenitor5

Progenitors4

Molecular Biology1

Transplantation studies1

Genes10

Kirkeby1

Apoptosis1

Undergo2

Proliferative4

Transcriptional1

Protocols2

Bone marrow5

ESCs4

Functional8

Mechanisms4

Differentiate into various1

Assay3

Cardiomyocytes1

Spectroscopic1

Markers2

Therapies7

Precursors1

20161

Somatic cells1

Clinical6

Epiblast2

Generate1

Gene9

IPSC lines1

Fate2

Proteins5