• regulates
  • For example, H19 lncRNA cis-regulates IGF2 gene expression at the same genomic locus, and HOTAIR lncRNA is transcribed on Chr 12, and it transregulates HoxD gene on Chr 2. (hindawi.com)
  • The Wnt signaling pathway regulates Wnt-responsive genes through the stabilization of the cytoplasmic component β-catenin, which subsequently enters the nucleus and complexes with the T-cell factor (TCF)/lymphoid-enhancing factor family of transcription factors, whose activity is promoted by their association with β-catenin. (aacrjournals.org)
  • isozymes
  • We recently reported on the significant cooverexpression of PLD isozymes with β-catenin in human colorectal cancers and induced the expression of PLD1 by tumor promoter phorbol 12-myristate 13-acetate (PMA) for the enhancement of the activity of MMP ( 5 ). (aacrjournals.org)
  • interaction
  • Furthermore, we identified differentially expressed lncRNAs that are co-located and co-expressed with differentially expressed coding RNAs in ESCC and the results point to a potential interaction between lncRNAs and neighboring coding genes that affect ether lipid metabolism, and the interaction may contribute to the development of ESCC. (hindawi.com)
  • novel
  • The present results implicate PLD1 as a novel target of Wnt3a, one of the Wnts-mediated canonical signaling pathway that promotes Wnt/β-catenin activity, which in turn enhances anchorage-independent growth and invasion in concert with Wnt/β-catenin signaling. (aacrjournals.org)
  • We aimed to generate novel renal genome-wide gene transcription data in rat models of diabetes in order to test the responsiveness to hyperglycaemia and renal structural changes of positional candidate genes at selected diabetic nephropathy (DN) susceptibility loci. (biomedcentral.com)
  • Further expression analysis of rat orthologs of human DN positional candidate genes provided functional annotations of known and novel genes that are responsive to hyperglycaemia and may contribute to renal functional and/or structural alterations. (biomedcentral.com)
  • protein
  • LncRNAs are generally expressed at a lower level than protein-coding genes, and they display more tissue-specific and cell-specific expression patterns [ 8 , 9 ]. (hindawi.com)
  • PLD1 (NP_002653, 965 a.a. ~ 1074 a.a) partial recombinant protein with GST tag. (abnova.com)
  • These included genes already tested in genetic studies of DN and a large number of protein coding sequences of unknown function which can be considered as functional and, when they map to DN loci, positional candidates for DN. (biomedcentral.com)
  • Phospholipase D3, also known as PLD3, is a protein that in humans is encoded by the PLD3 gene. (wikipedia.org)
  • PLD3 was first characterized as a human homolog of the HindIII K4L protein in the vaccinia virus, having a DNA sequence 48.1% similar to the viral gene. (wikipedia.org)
  • Ras-related protein Ral-A (RalA) is a protein that in humans is encoded by the RALA gene on chromosome 7. (wikipedia.org)
  • upstream
  • Phospholipase D1 (PLD1) is upstream in the mTOR pathway and serves as a positive effector for mTORC1. (wikipedia.org)
  • The genomic coordinate information of 410 DE-lncRNAs was used to search neighboring genes whose genomic locations are within ~5 kb upstream and ~1 kb downstream of the lncRNA and may extend to 1000 kb in both directions using GREAT software (http://bejerano.stanford.edu/great/public/html/index.php). (nih.gov)
  • Genomic location shows that PLD1 is located at −22,068 bp upstream of ESCCAL-337 lncRNA on Chr 3 and LPCAT1 is at −21,250 bp upstream of ESCCAL-356 lncRNA on Chr 5. (nih.gov)
  • cardiac
  • More than half a century of biochemical studies have implicated phospholipase D and PA activity in a wide range of physiological processes and diseases, including inflammation, diabetes, phagocytosis, neuronal & cardiac signaling, and oncogenesis. (wikipedia.org)
  • 19q13.2
  • The PLD3 gene in humans is located at chromosome 19q13.2, with a sequence comprising at least 15 exons and is alternatively spliced at the low GC 5' UTR into 25 predicted transcripts. (wikipedia.org)
  • PLD3
  • The PLD3 gene is also found with high expression in the cerebellum. (wikipedia.org)
  • PLD3 was implied to be involved in sensing oxidative stress, such that suppressing the PLD3 gene with short hairpin RNA increased the viability of cells exposed to oxidative stress. (wikipedia.org)
  • endocytosis
  • For example, dynamin and μ2 showed the effects on EGFR -mediated endocytosis via R128/R197 of the PLD1-PX domain and R304 of the PLD1-PH domain, respectively (Lee et al. (atlasgeneticsoncology.org)
  • Phospholipase D is an important player in many physiological processes, including membrane trafficking, cytoskeletal reorganization, receptor-mediated endocytosis, exocytosis, and cell migration. (wikipedia.org)
  • Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. (antibodies-online.com)
  • degradation
  • Miltefosine is almost completely eliminated by degradation via phospholipase D. Drug keeps accumulating until the end of treatment due to the extremely slow elimination, as seen by the long elimination half lives. (drugbank.ca)
  • proteins
  • Tuberous sclerosis is an autosomal recessive disease in which the genes required to express the tumor-suppressant proteins that form the TSC complex is mutated or missing, so the TSC complex is unable to function properly. (wikipedia.org)
  • humans
  • Combining transcriptomics in animal models and comparative genomics provides important information to improve functional annotations of disease susceptibility loci in humans and experimental support for testing candidate genes in human genetics. (biomedcentral.com)
  • RHEB is expressed by the RHEB gene in humans. (wikipedia.org)
  • expression
  • Tumorigenesis is a complex dynamic biological process that includes multiple steps of genetic and epigenetic alterations, aberrant expression of noncoding RNA, and changes in the expression profiles of coding genes. (hindawi.com)
  • Long noncoding RNAs (lncRNAs) are pervasively transcribed in the genome and they have key regulatory functions in chromatin remodeling and gene expression. (hindawi.com)
  • Mechanisms of action of transcribed lncRNAs are described as modifying chromatin architecture and regulating gene expression in a cis or trans manner. (hindawi.com)
  • Deregulation of β-catenin leads to the formation of β-catenin-TCF complexes and altered expression of TCF target genes, which can then contribute to the development of cancer ( 18 , 19 ). (aacrjournals.org)
  • Western Blot analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3. (abnova.com)
  • ddPCR ™ probe assay designed for gene expression analysis. (bio-rad.com)
  • The impact of prolonged hyperglycaemia on gene expression changes was more profound in STZ-WKY rats than in GK rats and involved largely different sets of genes. (biomedcentral.com)
  • antibody
  • Mouse monoclonal antibody raised against a partial recombinant PLD1. (abnova.com)
  • Detection limit for recombinant GST tagged PLD1 is 0.03 ng/ml as a capture antibody. (abnova.com)
  • locus
  • We wondered whether such a "locus control" process could operate in ESCC development, and, therefore, we searched neighboring genes of the 410 DE-lncRNAs in the genome. (nih.gov)
  • genome
  • Although genome-wide linkage studies have identified regions of the human genome contributing to DN [ 8 - 12 ], the causative genes remain unknown. (biomedcentral.com)
  • Identification
  • Identification of a Wnt-β-catenin-TCF-regulated PLD1 axis may provide new mechanistic insights into development of cancer. (aacrjournals.org)
  • Identification of lncRNAs co-located and co-expressed neighboring genes in esophageal squamous cell carcinoma (ESCC). (nih.gov)
  • a) Identification of neighboring genes of the DE-lncRNAs. (nih.gov)
  • lncRNAs
  • The percentage of DE-lncRNAs harboring zero, one, or two neighboring genes is presented. (nih.gov)
  • Overlap of 538 DE-lncRNA co-located genes with 3307 DE-mRNAs in microarrays identified 76 lncRNAs co-located and co-expressed coding mRNAs (list in Table 2). (nih.gov)
  • specific
  • Nephropathy does not necessarily develop in a significant proportion of diabetic patients, suggesting the involvement of specific genes. (biomedcentral.com)