Platelet aggregation. Medical search. Frequent questions

Agonists Abolished platelet activation Inhibition Inhibitors Flow cytometry Human platelet aggregation Thrombin Involved in platelet aggregation and activation Secretion Receptor Thrombus formation Fibrinogen Pathways Coagulation Proteins Inhibits Performed in platelet-rich Reduce platelet aggregation Receptors Aggregates Antibodies Mediates platelet Thrombocytopenia Arterial thrombosis Glycoprotein Extent of platelet Methods Fibrin Collagen-induced platelet Arachidonic Discoid platelets Activate platelets Anti-thrombotic Interactions with platelets Thromboxane Residual platelet Thrombosis Changes in platelet Adhesion of platelets Aggregometry Adenosine diphosphate Willebrand Vivo Analyzer Epinephrine Correlation Disorders Mechanisms Aggregometer Endothelial Calcium TRAP Blood Revealed by adenosine Membrane Clump together Morphological change Mechanism Reduced ADP-induced Activators Plasma Function

Platelet aggregometry is a standard clinical technique that measures the in vitro aggregability of platelets in response to agonists by using the amount of light transmitted through platelet-rich plasma (PRP) ( Holmsen, 1987 ). (nature.com)
Severe abnormalities in platelet function, such as Bernard-Soulier syndrome, Glanzmann's thrombasthenia, or platelet storage-pool deficiency, are characterized by the inability of platelets to aggregate in response to certain agonists. (nature.com)
It has long been assumed that platelet aggregation and thrombus growth are initiated by soluble agonists generated at sites of vascular injury. (nature.com)
By using high-resolution intravital imaging techniques and hydrodynamic analyses, we show that platelet aggregation is primarily driven by changes in blood flow parameters (rheology), with soluble agonists having a secondary role, stabilizing formed aggregates. (nature.com)
Platelet aggregation was measured in 55 patients undergoing elective PCI at 16-24 hours after 600 mg clopidogrel loading dose by light transmittance aggregometry using ADP 20 μM and thrombin receptor agonist peptide (TRAP) at 15 μM and 25 μM as agonists. (nih.gov)
After initial sample preparation (preparing PRP and PPP plasma) and loading, systems can perform platelet aggregation testing automatically for five agonists without additional pipetting steps or other dedicated hands-on time. (siemens-healthineers.com)
This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with other agonists in the vicinity of vessel injury, such as adenosine 5'-diphosphate (ADP), subendothelial collagen, and thrombin. (medscape.com)
Platelet aggregation studies offer a qualitative assessment of platelet function in response to various agonists that stimulate platelet activation pathways. (nwlpathology.nhs.uk)
To determine the contribution of platelet-leukocyte interactions to aggregation induced by platelet agonists, we performed standard impedance and optical aggregometry assays in the presence and absence of antibodies to P-selectin and P-selectin glycoprotein ligand 1 (PSGL). (johnshopkins.edu)
Specifically, we examined the dose-response nature of each individual's responsiveness to platelet agonists in parallel assays of platelet aggregation (assessed by light transmission in PRP) and platelet ATP secretion (assessed by luminometry). (isth.org)
The results show significant inter-individual differences in aggregation and secretion responses to all 3 agonists in the donor population. (isth.org)
There is a strong positive correlation between responses to all 3 agonists in the aggregation assays and, separately, in the secretion assays. (isth.org)
Platelets exhibit diverse responses in a variety of agonists. (edu.pk)
In vivo, most of the agonists act in synergism, causing aggregation of platelets. (edu.pk)
Results: The combinations of these agonists in subthreshold levels showed the synergistic responses in causing platelet aggregation. (edu.pk)
Conclusion: These findings indicate that the optimal platelet aggregation does occur between the above mentioned pairs of agonists even when each of the agonist is added to the other in subthreshold doses. (edu.pk)
Characteristics of the synergistic actions of platelet agonists. (edu.pk)
Our study presents compelling evidence in support of non-canonical juxtacrine Notch signaling within platelet aggregates that synergizes with physiological agonists to generate occlusive intramural thrombi. (elifesciences.org)
aggregation was evaluated by whole blood platelet aggregometry employing Multiplate Analyzer (agonists: arachidonic acid and collagen) and VerifyNow Aspirin. (crispr-reagents.com)
Alibertia edulis leaf extract considerably inhibited platelet aggregation induced by completely different agonists. (lindenbachlab.org)
Studies performed included platelet count, immature platelet fraction (IPF), plasma thrombopoietin (TPO), C-reactive protein (CRP), platelet aggregation and ATP secretion in response to six agonists, and spontaneous aggregation. (umn.edu)

Blockade of the platelet Fc-receptor or enzymatic cleavage of IgG abolished platelet activation and aggregation. (lu.se)

Clopidogrel inhibits ADP mediated platelet aggregation through inhibition of the P2Y12 receptor by its active metabolite. (nih.gov)
Thrombin induces platelet aggregation by binding to protease activated receptor-1 (PAR-1), and inhibition of PAR-1 has been evaluated in patients treated with clopidogrel to reduce ischemic events after acute coronary syndromes. (nih.gov)
We tested whether low-dose sevoflurane inhalation provides sustained inhibition of detrimental granulocyte-platelet aggregation in humans. (uzh.ch)
Inhibition of granulocyte-platelet aggregates was accompanied by reduced clot firmness 24 h after sevoflurane inhalation compared with control. (uzh.ch)
It has now been found that certain compounds within the scope of International Patent Application WO 9905143 but not specifically disclosed therein exhibit high potency combined with surprisingly high metabolic stability and bioavailibility, such that the predicted therapeutic dose for prolonged inhibition of aggregation in man shows advantage. (justia.com)
The inhibition of platelet aggregation and blood coagulation by Micropechis ikaheka venom. (ox.ac.uk)
DLL-4, in turn, instigated neighbouring platelets to switch to 'activated' phenotype through cleavage of Notch receptor and release of its intracellular domain (NICD), which was averted by inhibition of γ-secretase and phosphatidylinositol-3-kinase (PI3K). (elifesciences.org)
Inhibition of Notch signaling, too, restrained agonist-induced platelet activation, and significantly impaired arterial thrombosis in mice. (elifesciences.org)
This may reflect that some patients do not derive adequate platelet inhibition from Hydroxocobalamin (Vitamin B12a) aspirin [1]-[2]. (crispr-reagents.com)
In the search for genetic mechanisms to explain inadequate platelet inhibition by aspirin especially the IIIa subunit of the complex continues to be scrutinized [10]. (crispr-reagents.com)
Our outcomes reveal the antiplatelet aggregation properties of Alibertia edulis leaf extract, rutin and caffeic acid offering pharmacological details about its origin from cyclooxygenase 1 inhibition and its downstream pathway. (lindenbachlab.org)

This was also seen when preactivated platelets, which cannot undergo further morphological changes, were used, and when platelets were activated in the presence of shape-change inhibitors such as cytochalasin D and vincristine. (nature.com)
Platelets and serum were treated with different inhibitors to determine mechanisms involved in platelet aggregation and activation. (lu.se)

Aggregation was studied using light-transmission aggregometry and platelet activation using flow cytometry detecting. (lu.se)
Aggregation was studied using light-transmission aggregometry and platelet activation using flow cytometry detecting surface upregulation of CD62P. (lu.se)
Venous blood samples were collected at baseline before inhalation, immediately after inhalation, and 24 h thereafter, and were used for flow cytometry to determine platelet surface marker (CD41, CD42b, CD62P/P-selectin, and PAC-1) on platelets and granulocytes and for kaolin-induced clot formation, as assessed by thromboelastography. (uzh.ch)
In flow cytometry experiments, platelets were stimulated with arachidonic acid (AA, 30 microM), adenosine diphosphate (ADP, 1 microM), and thrombin receptor agonist peptide-6 (TRAP-6, 6 microM). (uzh.ch)
Transcripts/peptides of Notch1 and Delta-like ligand (DLL)-4 were detected in platelets isolated from human blood by RT-qPCR, Western analysis and flow cytometry. (elifesciences.org)
Platelet aggregation, granule secretion and platelet-leukocyte interaction were analyzed by lumi-aggregometry and flow cytometry. (elifesciences.org)
further testing may include platelet aggregation tests, platelet flow cytometry, and in some cases, genetic testing. (arupconsult.com)

The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
Effects of time, platelet concentration and sex on the human platelet aggregation response. (edu.pk)

Platelets were activated with ADP, thrombin, or the thrombin receptor agonist peptide SFLLRN (TRAP 1-6 ). (nature.com)
Figure 2: Platelet aggregation induced shear microgradients occurs independently of ADP, TXA 2 and thrombin. (nature.com)
Thrombin can produce platelet aggregation largely independently of other pathways but substantial quantities of thrombin are unlikely to be present without prior activation of platelets by other mechanisms. (justia.com)
Platelet aggregation studies test the clumping response of platelets to various platelet activators (eg, ADP, collagen, arachidonic acid, thrombin, epinephrine, ristocetin) as continuously recorded by a light transmission aggregometer. (medscape.com)
Whole blood aggregation in response to thrombin receptor activating peptide (TRAP) was determined by impedance aggregometry. (johnshopkins.edu)
Strikingly, prevention of DLL-4-Notch1 interaction by a blocking antibody abolished platelet aggregation and extracellular vesicle shedding induced by thrombin. (elifesciences.org)
The blood samples were stimulated by adenosine diphosphate (8 μ M) for evaluation of platelet-monocytic THP1 cell aggregation and P-selectin expression and were stimulated by thrombin (0. (tmu.edu.tw)
Platelet ATP secretion was similar in the two groups, except the response to 50μmol/l thrombin receptor agonist peptide 6 (TRAP 6) was significantly greater in GWVI+ patients. (umn.edu)

Genes involved in platelet aggregation and activation are downregulate" by Francesca M. McGrath, Abbie Francis et al. (edu.au)
Conclusion: Downregulation of genes involved in platelet aggregation and activation is a unique feature of the early anaphylactic reaction not previously reported and may be associated with reaction severity. (edu.au)

Their effect on oxidative stress, platelet aggregation and the secretion of cytokines from mononuclear cells was measured and a biological score was calculated. (wineinformationcouncil.eu)
Seven tubes are required for full aggregation and secretion testing. (medscape.com)
With some aggregometers, the secretion of platelet granules, another indicator of platelet function, may also be evaluated simultaneously by measuring the release of ATP by the aggregating platelets. (medscape.com)
Platelet secretion defects can provide greater diagnostic sensitivity than platelet aggregation testing alone. (medscape.com)
The result is the secretion of ADP and thromboxane A2 which subsequently activate other platelets. (teachmephysiology.com)
Similarly, the maximal extent of ATP secreted in response to TRAP from minimum 0.11 to maximum 4.67 pmoles / 106 platelets, demonstrating a 40 to 50 fold range in secretion-capacity for this dense-granule component. (isth.org)
However, there is no correlation between platelet secretion and aggregation responses. (isth.org)
Aggregation and secretion are independently regulated, suggesting that more studies are merited to investigate if ATP secretion might provide better insights into clinical risks in patient populations. (isth.org)

citation needed] In an unknown fashion, the antibiotic ristocetin causes von Willebrand factor to bind the platelet receptor glycoprotein Ib (GpIb), so when ristocetin is added to normal blood, it causes agglutination of fixed platelets or initiates the initial agglutination phase of aggregation of live platelets. (wikipedia.org)
Savage, B., Almus-Jacobs, F. & Ruggeri, Z.M. Specific synergy of multiple substrate-receptor interactions in platelet thrombus formation under flow. (nature.com)
SBSEC mediates platelet aggregation in an IgG and IgG-Fc-receptor dependent manner. (lu.se)
The Fc-IgG-receptor pathway was shown essential for platelet activation and aggregation. (lu.se)
It adds SBSEC to a group of bacteria that activate and aggregate platelets via the platelet Fc-receptor. (lu.se)
ADP-induced platelet aggregation is mediated by the P 2T receptor subtype located on the platelet membrane. (justia.com)
The P 2T receptor (also known as P2Y ADP or P2T AC ) is primarily involved in mediating platelet aggregation/activation and is a G-protein coupled receptor which is as yet uncloned. (justia.com)
Adhesion receptors promote the adhesion of platelets to other platelets, the vessel wall or leucocytes , depending on the receptor stimulated. (teachmephysiology.com)
Once activated, platelets express the GPIIb/IIIa receptor which can then bind with vWF or fibrinogen. (teachmephysiology.com)
Scholars@Duke publication: Genetic Variation in the Platelet Endothelial Aggregation Receptor 1 Gene Results in Endothelial Dysfunction. (duke.edu)
Platelet Endothelial Aggregation Receptor 1 (PEAR1) is a newly identified membrane protein reported to be involved in multiple vascular and thrombotic processes. (duke.edu)
While most studies to date have focused on the effects of this receptor in platelets, PEAR1 is located in multiple tissues including the endothelium, where it is most highly expressed. (duke.edu)
Synergistic responses and receptor occupancy in rabbit blood platelets. (edu.pk)
Results The A-allele of the rs12041331 SNP in the platelet endothelial aggregation receptor-1 ((rs12041331) reproducibly influenced platelet aggregation in aspirin-treated patients with coronary artery disease. (crispr-reagents.com)
The platelet surface hosts a panel of receptors mediating platelet activation and ultimately they all converge towards glycoprotein (GP) IIb/IIIa fibrinogen receptor complex. (crispr-reagents.com)

describes a novel signaling pathway involving Notch1 and its ligand, Δ-like ligand-4 (DLL4) in driving platelet activation and thrombus formation. (elifesciences.org)
An antiplatelet is a medication that decreases platelet aggregation and inhibits thrombus formation. (cdc.gov)

Savage, B., Saldivar, E. & Ruggeri, Z.M. Initiation of platelet adhesion by arrest onto fibrinogen or translocation on von Willebrand factor. (nature.com)
Fibrinogen mediated activation of platelet aggregation. (bmj.com)
The underlying mechanisms to explain these protective effects against coronary heart disease include an increase in high-density lipoprotein cholesterol and an increase in insulin sensitivity, and a decrease in platelet aggregation and circulating concentrations of fibrinogen. (wineinformationcouncil.eu)
Whatever the initial stimulus, the final common event is a cross-linking of platelets by binding of fibrinogen to a membrane-binding site, glycoprotein IIb/IIIa (GPIIb/IIIa). (justia.com)
On resting platelets, GP IIb/IIIa is unable to bind fibrinogen or vWf. (medscape.com)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)
Fibrinogen facilitates the formation of crosslinks between platelets, aiding platelet aggregation to form a platelet plug. (teachmephysiology.com)
Fibrinogen is an essential blood-clotting factor and is involved in a range of other functions, including platelet aggregation and smooth muscle proliferation. (cdc.gov)

The Streptococcus bovis/Streptococcus equinus complex (SBSEC) contains known IE-pathogens, but there is limited research on the different subspecies ability to interact with platelets and what signaling pathways are involved. (lu.se)
A number of converging pathways lead to platelet aggregation. (justia.com)
Platelet activation pathways. (medscape.com)
However given the considerable interdependency of platelet activation pathways and the fact that aspirin has effects impartial of COX-1 [8]-[9] the effect of aspirin may also be susceptible to genetically decided changes of various other receptors. (crispr-reagents.com)

Integrated onto routine coagulation analyzers-the Sysmex® CS-2500 and CS-5100 Systems 1 and the Atellica® COAG 360 System 2 -platelet aggregation becomes more manageable, reliable, and accessible with easy-to-use, standardized testing. (siemens-healthineers.com)
Test scheduling is simplified, because platelet aggregation testing can be performed by any lab technician at any time on a routine coagulation analyzer. (siemens-healthineers.com)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
Platelet disorders lead to defects in primary hemostasis and produce signs and symptoms different from coagulation factor deficiencies (disorders of secondary hemostasis). (medscape.com)
Platelets are generally considered as the main functional unit of the coagulation system. (qxmd.com)
Problems can occur when platelets are low in number or do not work properly or when certain coagulation factors are low or missing. (medlineplus.gov)
70%. Severe cases exhibit abnormal coagulation, endothelial barrier disruption, and dysfunctional platelet aggregation but the underlying mechanisms remain poorly understood. (cdc.gov)
In Sierra Leone during 2015-2018, we assessed LF patients' day-of-admission plasma samples for levels of proteins necessary for coagulation, fibrinolysis, and platelet function. (cdc.gov)
During the process, increased platelet aggregation and coagulation. (msdmanuals.com)

Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
Normal blood clotting involves blood particles, called platelets, and as many as 20 different plasma proteins that layer over the platelets. (medlineplus.gov)

Novel evidence is provided demonstrating that adiponectin has anti-thrombotic properties, since it inhibits platelet aggregation and platelet activation. (cun.es)
1 vol % end-tidal) inhibits agonist-induced granulocyte-platelet interactions 24 h after administration and thus counteracts thromboinflammatory processes. (uzh.ch)

Also, in vitro studies were performed in platelet-rich plasma from 9 healthy volunteers. (cun.es)
Optical aggregometry was performed in platelet rich plasma (PRP)in the presence and absence of isolated granulocytes. (johnshopkins.edu)

Most epidemiological studies have found sustained consumption at light-to-moderate amounts to increase HDL cholesterol, reduce platelet aggregation, and promote fibrinolysis. (wineinformationcouncil.eu)

Platelets express the receptors for adiponectin. (cun.es)
Platelets have abundant surface receptors , classified into agonist and adhesion receptors. (teachmephysiology.com)
Exposed collagen fibres bind vWF released from the damaged endothelium, which in turn binds to vWF receptors on platelets to promote adhesion. (teachmephysiology.com)
When a platelet binds to collagen, the glycoprotein IIb/IIIa pathway is activated - a complex system controlled by G-protein coupled receptors (GPCRs). (teachmephysiology.com)

We find that in response to vascular injury, thrombi initially develop through the progressive stabilization of discoid platelet aggregates. (nature.com)
Analysis of blood flow dynamics revealed that discoid platelets preferentially adhere in low-shear zones at the downstream face of forming thrombi, with stabilization of aggregates dependent on the dynamic restructuring of membrane tethers. (nature.com)
The amount of granulocyte-platelet aggregates increased upon agonist stimulation. (uzh.ch)
Low-dose sevoflurane inhalation reduced ADP-induced CD62P expression on platelets 24 h after inhalation, and inhibited the formation of granulocyte-platelet aggregates under stimulation with AA and ADP after 1 and 24 h, and with TRAP-6 after 24 h compared with control. (uzh.ch)
and the number of platelet-neutrophil aggregates increased significantly at 12 hours. (tamu.edu)
CONCLUSIONS AND CLINICAL RELEVANCE: In horses, concurrent increases in venous blood ET-1 immunoreactivity, insulin and glucose concentrations, and platelet-neutrophil aggregates support a role of endothelial dysfunction in the pathogenesis of CHO-induced laminitis. (tamu.edu)

CD69 stimulation by anti-Leu-23 monoclonal antibodies induced platelet aggregation in a dose-dependent fashion. (nih.gov)
The high anti-platelet efficacy of antibodies or antagonists for GPIIb/IIIa is explained by their interference with this final common event. (justia.com)
Aggregation was performed in the presence and absence of antibodies to P-selectin, PSGL, or an IgG control. (johnshopkins.edu)

The platelet GP IIb/IIIa complex mediates platelet-to-platelet interactions (platelet aggregation). (medscape.com)

The workup of a patient with a platelet-type bleeding pattern typically includes correlation with platelet count ( thrombocytopenia ), evaluation of platelet morphology on peripheral smear, exclusion of acquired causes of platelet dysfunction, and testing for von Willebrand disease (VWD) . (arupconsult.com)
Thrombocytopenia is a common feature of hemorrhagic fevers and vascular permeability disorders ( 8 ), but the decrease in platelet counts in acute LF is not low enough to cause spontaneous hemorrhage. (cdc.gov)
A low platelet count is called thrombocytopenia. (healthline.com)
Whether or not you experience symptoms of thrombocytopenia may depend on how low your platelet count is. (healthline.com)
Platelets live about 7-10 days in people without thrombocytopenia. (healthline.com)

Platelet aggregation at sites of vascular injury is essential for hemostasis and arterial thrombosis. (nature.com)
to Platelet adhesion and aggregation are initiating events in arterial thrombosis. (justia.com)
Platelet activation plays an important role in arterial thrombosis in coronary heart disease, stroke, and peripheral arterial disease. (isth.org)

Distinct glycoprotein Ib/V/IX and integrin αIIbβ3-dependent calcium signals cooperatively regulate platelet adhesion under flow. (nature.com)
Platelets play a primary role in this process, interacting with subendothelium-bound von Willebrand factor (vWf) via the membrane glycoprotein (GP) Ib complex. (medscape.com)

Since then, the initial decrease in light transmission detected in aggregometry is used as a measure for the extent of platelet shape change. (nature.com)

Subjects/Methods: Platelet aggregation and circulating adiponectin, sCD40L and P-selectin were determined in 30 controls and 30 patients with MS. (cun.es)
Methods: In this study, the synergism between ADP - Epinephrine and Epinephrine - Collagen has been determined by means of turbidometric method, which measures changes in optical density of platelet suspension. (edu.pk)
Methods: Platelet-rich plasma samples from healthy volunteers were treated with cilostazol. (tmu.edu.tw)

The initial hemostatic plug, composed primarily of platelets, is stabilized further by a fibrin mesh generated in secondary hemostasis. (medscape.com)

Cytosolic calcium as a second messenger for collagen-induced platelet responses. (edu.pk)
All the steps in ADP induced but not collagen induced platelet activation, viz. (caluniv.ac.in)

In addition, CD69 stimulation in platelets induced production of thromboxane B2 and PGE2, suggesting activation of arachidonic acid metabolism by cycloxygenase. (nih.gov)

We present a microfluidic device that is able to trigger initial recruitment and subsequent aggregation of discoid platelets by mimicking the effects of pathological changes in blood vessel geometry. (technologynetworks.com)

These interactions further activate platelets. (medscape.com)

The limited anti-thrombotic efficacy of aspirin may reflect the fact that it blocks only one source of ADP which is that released in a thromboxane-dependent manner following platelet adhesion (see e.g. (justia.com)
Thus, Notch pathway can be a potential anti-platelet/anti-thrombotic therapeutic target. (elifesciences.org)

Twenty-six isolates of the four most common species and subspecies of SBSEC identified in bacteremia were collected, and interactions with platelets were investigated in platelet rich plasma (PRP) from three donors. (lu.se)

Drugs or agents which antagonize or impair any mechanism leading to blood platelet aggregation, whether during the phases of activation and shape change or following the dense-granule release reaction and stimulation of the prostaglandin-thromboxane system. (online-medical-dictionary.org)
Elevated platelet count, C-reactive protein and thromboxane analog-induced platelet aggregation in patients with Gulf War veterans' illnesses: Evidence of a chronic inflammatory state? (umn.edu)

Non-responders to clopidogrel show increased residual platelet aggregation induced by TRAP, whereas clopidogrel responders exhibit attenuated response to TRAP. (nih.gov)
Furthermore heritable factors take into account around 30% from the variant in innate platelet reactivity [5] and Hydroxocobalamin (Vitamin B12a) hereditary variability can be an essential contributor to residual platelet reactivity during aspirin treatment [6]. (crispr-reagents.com)

Background: Platelet activation is critical during inflammation, thrombosis, and tumor progression and metastasis. (tmu.edu.tw)

The exact biological mechanism remains elusive but the effect of this polymorphism may be related to changes in platelet activation. (crispr-reagents.com)

The body's reaction to vessel wall injury is rapid adhesion of platelets to the subendothelium. (medscape.com)

Because the initial decrease in light transmission in platelet aggregometry is attributed to platelet shape change, it is widely held that platelet shape change is a prerequisite for platelet aggregation. (nature.com)

Michal F, Motamed M. Shape change and aggregation of blood platelets: interaction between the effects of adenosine diphosphate, 5-hydroxytryptamine and adrenaline. (edu.pk)

It measures platelet aggregation with the help of von Willebrand factor (vWF) and exogenous antibiotic ristocetin added in a graded fashion. (wikipedia.org)
It is similar to the ristocetin cofactor assay but has the added benefit in that it helps in the diagnosis of type 2B/pseudo von Willebrand disease (vWD) and Bernard-Soulier syndrome because it uses patient's live endogenous platelets, whereas ristocetin cofactor assay tests the function of only the vWF and not the platelets. (wikipedia.org)
Von Willebrand disease and hemorrhagic abnormalities of platelet and vascular function. (medlineplus.gov)

The ristocetin-induced platelet aggregation (RIPA) is an ex vivo assay for live platelet function. (wikipedia.org)
Figure 1: Shear microgradients promote platelet aggregate formation in vivo . (nature.com)

SC-2000 semi-automated platelet aggregation analyzer uses 100-220V. (succeeder.com)
Platelet-derived extracellular vesicles were documented with Nanoparticle Tracking Analyzer. (elifesciences.org)

ADP- and epinephrine platelet aggregation was measured after preincubation with adiponectin. (cun.es)
Higher platelet aggregation was found in the MS. Adiponectin inversely correlated with P-selectin (R:-0.35, p:0.009), sCD40L (R:-0.24, p:0.05) and epinephrine and collageninduced aggregation (r=-0.80, p=0.005 and r=-0.70, p=0.011). (cun.es)

In the authors' previous study, it was reported that the degree of platelet aggregation in an aneurysm had a signifi cant correlation with the flow pattern in the aneurysmal dome. (witpress.com)
Research progress on the correlation between platelet aggregation and tumor progression. (qxmd.com)

700+ publications in peer-reviewed journals confirm the convenient and reliable testing of platelet-function disorders using Siemens Healthineers PFA Systems. (siemens-healthineers.com)
Integrated platelet aggregation provides cost-effective system consolidation for detection of inherited, acquired, or drug-induced platelet disorders. (siemens-healthineers.com)
Conditions associated with decreased platelet aggregation include suspected hereditary and acquired disorders of platelet function. (medscape.com)
Bleeding disorders can also result from a problem with the number or function of platelets. (medlineplus.gov)
Functional platelet disorders affect the adhesion, activation, or aggregation of platelets and are frequently associated with excessive bleeding, often following trauma or surgery. (arupconsult.com)
How do functional platelet disorders manifest clinically? (arupconsult.com)
The clinical features of functional platelet disorders vary. (arupconsult.com)
rarely, it may be observed in functional platelet disorders). (arupconsult.com)
Functional platelet disorders are generally associated with immediate bleeding in response to surgery or trauma, whereas factor deficiencies are associated with delayed bleeding. (arupconsult.com)
What are some causes of acquired functional platelet disorders? (arupconsult.com)
Which functional platelet disorders are inherited? (arupconsult.com)
Bernard-Soulier syndrome (an adhesion disorder) and Glanzmann thrombasthenia (an aggregation disorder) are among the most well-recognized inherited disorders of platelet function and are severe clinical conditions. (arupconsult.com)
Refer to the table, Laboratory Findings in Selected Inherited Functional Platelet Disorders , for additional information and a more comprehensive list of inherited functional platelet disorders. (arupconsult.com)
Laboratory testing for functional platelet disorders is appropriate in individuals with bleeding manifestations that suggest a functional platelet disorder (eg, easy bruising, prolonged bleeding following dental work or surgery, menorrhagia), particularly those with a family history of a bleeding disorder and in whom VWD has been previously excluded. (arupconsult.com)

These findings provide insight into the prothrombotic effects of disturbed blood flow parameters and suggest a fundamental reinterpretation of the mechanisms driving platelet aggregation and thrombus growth. (nature.com)
To the best of our knowledge, this is the first study that reports on platelet interactions of SBSEC-isolates other than Streptococcus gallolyticus subspecies gallolyticus as well as the first study to report of mechanisms of platelet interaction of SBSEC-isolates. (lu.se)
Once activated, platelets have two major mechanisms to recruit additional platelets to the growing hemostatic plug. (medscape.com)

AGEs quantification (spectrofluorometric method ) and the platelet aggregation test (aggregation of cuvettes in a four-channel platelet aggregometer) were also conducted. (bvsalud.org)

Although the process of platelet adhesion to the sub-endothelial surface may have an important role to play in the repair of damaged vessel walls, the platelet aggregation that this initiates can precipitate acute thrombotic occlusion of vital vascular beds, leading to events with high morbidity such as myocardial infarction and unstable angina. (justia.com)
The venom inhibited platelet aggregation in response to ADP and collagen, and also delayed clotting dependent on platelet activation or endothelial cell tissue factor expression. (ox.ac.uk)
These results suggest that, by inhibiting procoagulant activities of platelets and endothelial cells, a 17 kDa PLA2 plays an important role in the anticoagulant action of M. ikaheka venom. (ox.ac.uk)
The impaired homeostasis and platelet dysfunction implicate alterations in the protein C pathway, which might contribute to the loss of endothelial barrier function in fatal infections. (cdc.gov)

Intercellular calcium communication regulates platelet aggregation and thrombus growth. (nature.com)
Ishii, Umeda F, Hashimoto T, Nawata H. Increased intracellular calcium mobilization in platelets from patients with type 2 (non-insulin dependent) diabetes mellitus. (edu.pk)

Increasing quartiles of 20 μM ADP induced platelet aggregation after clopidogrel loading were associated with increasing levels of TRAP mediated platelet aggregation. (nih.gov)
Addition of PAR-1 antiplatelet drugs may be most effective in patients with reduced clopidogrel response and high residual TRAP mediated platelet aggregation. (nih.gov)
RESULTS: AA, ADP, and TRAP-6 markedly increased the expression of CD62P on platelets, whereas CD42b (shedding) and PAC-1 (heterotypic conjugates) expression decreased. (uzh.ch)

In this study, patterns of blood fl ow in three models of cerebral artery bifurcation aneurysms were numerically analysed and compared to discern the likelihood of platelet aggregation. (witpress.com)
These impingements presumably caused haemolysis, with ADP originating from haemolysed red blood cells inducing platelet aggregation. (witpress.com)
Aspirin has no effect on aggregation produced by other sources of ADP, such as damaged cells or ADP released under conditions of turbulent blood flow. (justia.com)
The platelets arise from the fragmentation of the cytoplasm of megakaryocytes in the bone marrow and circulate in blood as disc-shaped anucleate particles for 7-10 days. (medscape.com)
Platelets play an important role in the formation of blood clots . (teachmephysiology.com)
The key role of platelets is their participation in haemostasis through the formation of blood clots at the site of bleeding. (teachmephysiology.com)
Conclusions: The presence of granulocytes in whole blood and plasma contributes substantially to aggregation induced by platelet activators. (johnshopkins.edu)
Temperature and platelet aggregation during storage of whole blood. (docksci.com)
SjR,-Cold storage of platelet-rich plasma, platelet concen- platelet aggregation and reduced platelet viability.1-6 Nevertheless, although many centres now advise short-term storage of platelet concentrates and platelet-rich plasma at room temperature, whole blood is still stored refrigerated, partly because of the unknown effects of long term (up to 35 days) storage at room temperature and also to inhibit bacterial multiplication. (docksci.com)
At least one blood-pack manufacturer (Fenwall) advises storage of whole blood at 1-6°C. However, in our experience whole blood storage at 1°C is considerably worse, in respect of cellular aggregation, than trates, and whole blood can result in storage at 4°C, and at room temperature little or no aggregation is observed even after 21 days storage. (docksci.com)
Cellular aggregation was measured using the screen filtration pressure (S.F.P.) method7 on samples from standard units of blood. (docksci.com)
Background: Efficient hemostasis in human body depends on a complicated series of events which actively involve blood platelets. (edu.pk)
The aggregation of blood platelets. (edu.pk)
Effects of adrenaline on human blood platelets. (edu.pk)
The numbers of white blood cells and platelets may also be higher than normal. (limamemorial.org)
The platelet aggregation blood test checks how well platelets, a part of blood, clump together and cause blood to clot. (limamemorial.org)
When you don't have enough platelets in your blood supply, your body can't form clots. (healthline.com)
Your bone marrow is the spongy tissue inside the bone where all the components of blood, including platelets, are produced. (healthline.com)
If you think you may be experiencing symptoms of low platelet count, talk to a healthcare professional - especially if you're experiencing serious symptoms, such as wounds or cuts that won't stop bleeding, or blood in your stools or urine. (healthline.com)
We also observed host-dependent differences for interaction of platelets with C. albicans , showing enhanced platelet aggregation, adhesion and activation in murine blood. (frontiersin.org)
For human blood, opsonization was shown to decrease platelet interaction suggesting that complement factors interfere with fungus-to-platelet binding. (frontiersin.org)
platelet-neutrophil aggregation, and concentrations of nitric oxide, insulin, and glucose in blood obtained from horses following carbohydrate overload. (tamu.edu)
PROCEDURES: Digital and jugular venous blood samples were collected at 1-hour intervals (for assessment of endothelin-1 [ET-1] immunoreactivity and measurement of glucose, insulin, and nitric oxide [NO] concentrations) or 4-hour intervals (CBC and platelet-neutrophil aggregate assessment) for 8 hours or 16 hours after induction of CHO-associated laminitis in horses treated with an ET-1 antagonist. (tamu.edu)
Washed RBCs are free of almost all traces of plasma, most white blood cells, and platelets. (msdmanuals.com)
for exchange transfusions, for patients who require cytomegalovirus-negative blood that is unavailable, and possibly for the prevention of human leukocyte antigen (HLA) alloimmunization to help prevent refractoriness to platelet transfusion (failure to achieve the target level of blood platelets after platelet transfusion). (msdmanuals.com)

This effect was associated with Ca2+ influx and platelet degranulation, as revealed by adenosine triphosphate release. (nih.gov)

Figure 5: Stabilized discoid platelet aggregation occurs via restructuring of membrane tethers. (nature.com)
In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)
Platelet activation results in a morphological change on the membrane surface of the platelet, increasing the surface area and preparing it for aggregation. (teachmephysiology.com)

When your skin is injured or broken , your platelets clump together and form clots to stop the bleeding . (healthline.com)

It was suggested that this optical effect was caused by the morphological change of platelets from discs to spherical cells with pseudopodia ( Latimer et al, 1977 ). (nature.com)

Studies on the mechanism of ristocetin-induced platelet adhesion. (wikipedia.org)
For IE-pathogens, the capacity to activate and aggregate platelets is believed to be an important virulence mechanism. (lu.se)
Therefore, the present study explored the effects of M. ikaheka venom on platelets and endothelium, which are important components of the haemostatic mechanism. (ox.ac.uk)

In addition, an 11 kDa fraction containing a long-chain neurotoxin reduced ADP-induced aggregation. (ox.ac.uk)

The reference range is a normal biphasic pattern of aggregation in response to specific platelet activators (see image below). (medscape.com)

Ristocetin cofactor assay uses the patient's platelet poor plasma (with vWF but no platelets) and adds ristocetin and exogenous formalin-fixed platelets which can passively agglutinate (but not actively aggregate, as they are dead). (wikipedia.org)
Butt IF, Aslam M, Khan FA, Ayub M. Plasma insulin and platelet functions in diabetes mellitus. (edu.pk)
Vanags DM, Rodgers SE, Duncan EM, Lloyd JV, Bochner F. Potentiation of ADP induced aggregation in human platelet-rich plasma by 5-hydroxytryptamine and adrenaline. (edu.pk)
Fresh frozen plasma (FFP) is an unconcentrated source of all clotting factors without platelets. (msdmanuals.com)

These results suggest that CD69 may function as an activating molecule on platelets, as on lymphocytes, and point toward a more general role of this surface dimer in signal transduction. (nih.gov)
We hypothesized that adiponectin modulates platelet function. (cun.es)
Recovery time of platelet function after aspirin withdrawal. (medscape.com)
In this article, we will consider the structure, function and clinical relevance of platelets. (teachmephysiology.com)
Kunisaki M, Umeda F, Inoguchi T, Watanaba J, Nawata H. Effects of Vit-E administration on platelet function in diabetes mellitus. (edu.pk)
Cilostazol is widely used for arteriosclerosis obliterans and is known to inhibit platelet function. (tmu.edu.tw)
aspirin and nonsteroidal anti-inflammatory drugs (NSAIDs) are common causes of abnormal platelet function, and platelet function testing may need to be deferred if patients have been taking these medications. (arupconsult.com)
Results showed that coffee reduced AGE formation and platelet aggregation in our animal model , not altering the animals ' renal function. (bvsalud.org)
Platelet-dependent neutrophil function is dysregulated by M protein from Streptococcus pyogenes. (lu.se)

Anatomy15

Organisms14

Diseases16

Chemicals and Drugs144

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes46

Disciplines and Occupations1

Information Science2

Health Care1

Agonists21

Abolished platelet activation1

Inhibition11

Inhibitors2

Flow cytometry7

Human platelet aggregation2

Thrombin8

Involved in platelet aggregation and activation2

Secretion8

Receptor15

Thrombus formation2

Fibrinogen8

Pathways4

Coagulation10

Proteins2

Inhibits2

Performed in platelet-rich2

Reduce platelet aggregation1

Receptors4

Aggregates6

Antibodies3

Mediates platelet1

Thrombocytopenia5

Arterial thrombosis3

Glycoprotein2

Extent of platelet1

Methods3

Fibrin1

Collagen-induced platelet2

Arachidonic1

Discoid platelets1

Activate platelets1

Anti-thrombotic2

Interactions with platelets1

Thromboxane2

Residual platelet2

Thrombosis1

Changes in platelet1

Adhesion of platelets1

Aggregometry1

Adenosine diphosphate1

Willebrand3

Vivo2

Analyzer2

Epinephrine2

Correlation2

Disorders14

Mechanisms3

Aggregometer1

Endothelial4

Calcium2

TRAP3

Blood25

Revealed by adenosine1

Membrane3

Clump together1

Morphological change1

Mechanism3

Reduced ADP-induced1

Activators1

Plasma4

Function9