• coli
  • Members of Enterobacteriaceae family, for example, Escherichia coli or Klebsiella pneumoniae pose the biggest threat regarding plasmid-mediated resistance in hospital- and community-acquired infections. (wikipedia.org)
  • It appears that the overwhelming amount of host cell DNA isolated in the total DNA preparation effectively masks most of the rescuable plasmids when transforming E. coli DH10B (BRL). (bio.net)
  • Have you checked whether the coli is actually harbouring the plasmid? (bio.net)
  • In E. coli, multiple plasmid copies appear to cluster together, creating a few multiplasmid clusters in each cell. (wikipedia.org)
  • Two such mutants, PFF1cop254D and PFF1cop271C, increase the copy number of PFF1 in E. coli from approximately 39-40 to about 501 and 113 plasmids per cell, respectively. (wikipedia.org)
  • Experimental evidence has shown that in Escherichia coli, when levels of this RNA are decreased, the plasmid copy number of R1162 is increased. (wikipedia.org)
  • proteins
  • It also denatures proteins, like DNase, which is especially important if the plasmids are to be used for enzyme digestion. (wikipedia.org)
  • Partition systems involve three elements, organized in an auto-regulated operon: A centromere-like DNA site Centromere binding proteins (CBP) The motor protein The centromere-like DNA site is required in cis for plasmid stability. (wikipedia.org)
  • cDNA
  • Animal research has demonstrated that administration of HGF cDNA plasmids into ischemic cardiac tissue can increase cardiac function (improved left ventricular ejection fraction and fractional shortening compared to control subjects) after induced MI or ischemia. (wikipedia.org)
  • sequence
  • I am trying to find the sequence of some plasmids, but I have no idea who the vendor is, etc. (bio.net)
  • Malgorzata Adamczyk and Grazyna Jagura-Burdzy: "Spread and survival of promiscuous IncP-1 plasmids", Acta Biochimica Polonica, Vol 50, no. 2/2003, p. 425-453 LEWIS C. INGRAM, M. H. RICHMOND, AND R. B. SYKES: "Molecular Characterization of the R Factors Implicated in the Carbenicillin Resistance of a Sequence of Pseudomonas aeruginosa Strains Isolated from Burns", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 1973, p. 279-288 Thomas CM et al. (wikipedia.org)
  • miniprep
  • Try a colony miniprep to verify you have enough plasmid before performing the miniprep, although this technique doesn't work very well with low copy plasmids. (bio.net)
  • A typical plasmid DNA yield of a miniprep is 50 to 100 µg depending on the cell strain. (wikipedia.org)
  • Miniprep of large number of plasmids can also be done conveniently on filter paper by lysing the cell and eluting the plasmid on to filter paper. (wikipedia.org)
  • High Throughput miniprep of plasmid for sequencing Birnboim HC, Doly J (November 1979). (wikipedia.org)
  • Transfection
  • Ultrapure DNA purified with HiSpeed Plasmid Kits yields excellent results in all applications, from cloning and sequencing to transfection. (bio-medicine.org)
  • Transfection with HGF plasmids in damaged cardiac tissue also promotes angiogenesis (increased capillary density compared to control subjects), as well as decreasing detrimental remodelling of the tissue at the site of injury (decreased fibrotic deposition). (wikipedia.org)
  • It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. (wikipedia.org)
  • molecular
  • David Wessner reviews Vector NTI Suite, an INternet integrated software tool that can catalog, analyze, and design complex plasmids for molecular biologists. (sciencemag.org)
  • The term plasmid was introduced in 1952 by the American molecular biologist Joshua Lederberg to refer to "any extrachromosomal hereditary determinant. (wikipedia.org)
  • chromosome
  • Later in 1968, it was decided that the term plasmid should be adopted as the term for extrachromosomal genetic element, and to distinguish it from viruses, the definition was narrowed to genetic elements that exist exclusively or predominantly outside of the chromosome and can replicate autonomously. (wikipedia.org)
  • selective
  • The global spread of MDR plasmids has been enhanced by selective pressure from antibiotic usage in human and veterinary medicine. (wikipedia.org)
  • extrachromosomal
  • Plasmid publishes original research on genetic elements in all kingdoms of life with emphasis on maintenance, transmission and evolution of extrachromosomal elements . (elsevier.com)
  • genetic
  • A Ti or tumour inducing plasmid is a plasmid that often, but not always, is a part of the genetic equipment that Agrobacterium tumefaciens and Agrobacterium rhizogenes use to transduce their genetic material to plants. (wikipedia.org)
  • mechanism
  • The mechanism is similar to a treadmill mechanism: Multiple TubR dimer binds to the centromere-like region stbDRs of the plasmids. (wikipedia.org)
  • species
  • The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. (wikipedia.org)
  • transformation
  • I am sure that this is occurring based on experiments in which I dose the total DNA preparation with some control plasmid before transformation. (bio.net)
  • In the laboratory, plasmids may be introduced into a cell via transformation. (wikipedia.org)
  • Often
  • The plasmids are often coated with some type of adjuvant prior to delivery to enhance the immune response from the host. (wikipedia.org)