• Specifically, the work on host-controlled DNA modification and discovery of restriction endonucleases, which earned Arber the Nobel prize in Physiology or Medicine (along with Hamilton Smith and Daniel Nathans), was described in two 1962 articles where Dussoix and Arber were the sole authors. (wikipedia.org)
  • The integrity and the homogeneity of the viral DNA thus obtained was confirmed by restriction endonucleases analysis. (pasteur.fr)
  • Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome. (pasteur.fr)
  • The fragmentation of DNA using restriction endonucleases (REs). (openwetware.org)
  • The 6.7-kilobase plasmid (pTf1) was cloned separately into the HindIII or BamHI site of Escherichia coli plasmid pBR322. (unab.cl)
  • The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. (pasteur.fr)
  • A 4.8-kilobase SalI-EcoRI DNA fragment containing araE was subcloned from the phage lambda d(lysA+ galR+ araE+) into the plasmid vector pBR322. (ox.ac.uk)
  • Daisy Dussoix and Werner Arber showed that this process required enzymes, resulting in two publications that paved the way for discovery and isolation of the restriction and modification enzymes involved. (wikipedia.org)
  • Lab experiments introduce a variety of molecular biology techniques such as replica plating, bacterial conjugation and transformation, the isolation and restriction enzyme cleavage of plasmid DNA, and restriction mapping. (monroecc.edu)
  • Researchers can perform restriction enzyme analyses, edit sequences, create plasmid maps, cut and ligate "in silico," analyze GC content, make homology plots, and select primer sites from a user-specified database. (the-scientist.com)
  • Precise knowledge of restriction sites and removal of unnecessary sequences will facilitate plasmid manipulations and plant transformation. (tamu.edu)
  • cycFdr (founder) plasmid for uniform insertion of cyclin coding sequences. (galeriaespacio48.com)
  • A restriction map lists the locations of DNA sequences that are cut by a particular restriction. (solvedlib.com)
  • A restriction map lists the locations of DNA sequences that are cut by a particular restriction enzyme for a piece of DNA, such as a chromosome or a plasmid. (solvedlib.com)
  • The classical protocols to join DNA fragments by restriction and ligation as well as most techniques employing recombination depend on the presence of specific short sequences at or around the joining regions. (biomedcentral.com)
  • Digesting the DNA sequence with the restriction enzymes will result in fragmented DNA of predictable sizes, based on the restriction map, that allow a researcher to analyze if his or her construct was generated correctly when visualized using gel electrophoresis. (solvedlib.com)
  • She was one of the discoverers of restriction enzymes during her doctoral studies. (wikipedia.org)
  • She worked for her PhD with Werner Arber and Eduard Kellenberger, Swiss microbial geneticists, at the time when the barriers to infection of bacterial cells by virus (bacteriophage) first became apparent, leading to the discovery of restriction and modification enzymes that have subsequently become essential molecular biology tools. (wikipedia.org)
  • Dussoix-Roulland was a member of the research groups of two future Nobel Prizewinners (Werner Arber (for discovery of restriction enzymes), and the group of Harold Varmus and J. Michael Bishop (for the cellular origin of retroviral oncogenes). (wikipedia.org)
  • Use the linear restriction map below to predict where you would expect to see bands on a gel if a digest is performed using the specified restriction enzymes. (solvedlib.com)
  • Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium. (snapgene.com)
  • A basic plasmid vector map showing the restriction sites. (bitesizebio.com)
  • The presence of restriction sites within the insert makes it challenging to digest and clone into the MCS of the vector. (bitesizebio.com)
  • D) Schematic representation of pGS-3700 targeting plasmid containing a 3723 bp fragment viral genomic sequence including the viral cyclin from positions 101,654 and 105,377 (shown in reverse orientation here) in the pGS284 BAC recombination vector, and modifications to create the pGS3700. (galeriaespacio48.com)
  • Second, it can enable you to use Vector NTI Advance 11.0 with all its features and functions without any limitations or restrictions. (entrevart.com)
  • It can help you to use Vector NTI Advance 11.0 without paying for it and without any limitations or restrictions. (entrevart.com)
  • From this plasmid a 2.8-kilobase HincII-PvuII DNA fragment including araE was sequenced and also subcloned into the expression vector pAD284. (ox.ac.uk)
  • Brie﫿y, two single-stranded oligonucleotides are annealed to form a double-stranded DNA sequence containing the shRNA sequence followed by a Pol III terminator (consisting of a stretch of four or more thymidines), as well as 5'- and 3'-end "sticky" overhangs that are complementary to those generated in the targeting vector following restriction digestion with BglII and SalI (Figures 3E and 4C). (berterolab.com)
  • Hereditary congenital facial paresis type 1 (HCFP1) is an autosomal dominant disorder of absent or limited facial movement that maps to chromosome 3q21-q22 and is hypothesized to result from facial branchial motor neuron (FBMN) maldevelopment. (bvsalud.org)
  • High-resolution comparative mapping of the proximal region of the mouse X chromosome. (mpi.nl)
  • The murine homologues of the loci for McLeod syndrome (XK), Dent's disease (CICN5), and synaptophysin (SYP) have been mapped to the proximal region of the mouse X chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate Mus musculus x Mus spretus backcrosses. (mpi.nl)
  • These data have enabled us to construct a high-resolution genetic map for the approximately 3-cM interval between DXWas70 and Cybb on the mouse X chromosome, which encompasses 10 loci. (mpi.nl)
  • Addgene is a nonprofit plasmid repository. (addgene.org)
  • Imagine you wish to clone a PCR product that contains many common sticky end restriction endonuclease sites, such as EcoRI or HindIII, at the ends and within the sequence. (bitesizebio.com)
  • dH 2 O, DNA, Restriction Endonuclease (10U/µL) and its appropriate (10x) Buffer, incubator (i.e. a water bath) @ 37°C, ice, micro-centrifuge tube(s) (MCT) Optionally: Ribonuclease A (RNase), Alkaline Phosphatase (ALP), incubator @ 65°C. (openwetware.org)
  • Blunt-end cloning can be useful when traditionally sticky-end cloning isn't practical (such as if the presence of multiple restriction enzyme sites could cut up your fragment). (bitesizebio.com)
  • This will help make sense of the restriction pattern when run on an agarose gel and/or isolating a specific fragment for sub-cloning. (openwetware.org)
  • By using standard restriction fragment length polymorphism, 6 zero-copy IS 6110 Mycobacterium tuberculosis isolates were identified from 1,180 Maryland isolates as part of the National Tuberculosis Genotyping Surveillance Network Project. (cdc.gov)
  • IS 6110 restriction fragment length polymorphism (RFLP) has been used to investigate Mycobacterium tuberculosis transmission within populations since the early 1990s ( 1 - 4 ). (cdc.gov)
  • We analyzed travel-associated clinical isolates of Escherichia coli O104:H4, including 1 from the 2011 German outbreak and 1 from a patient who returned from the Philippines in 2010, by genome sequencing and optical mapping. (cdc.gov)
  • Analysis of plasmid, cosmid or genomic DNA in combination with various methods, e.g. (openwetware.org)
  • Potential recombinant clones were each screened by PCR, restriction mapping, and Southern blotting prior to final sequence verification. (galeriaespacio48.com)
  • Furthermore, the oligonucleotides are designed to destroy the BglII restriction enzyme site in the 﫿nal plasmid in order to allow ​screening of correct recombinant clones by diagnostic restriction digestion (Figure 4D). (berterolab.com)
  • Restriction maps of the recombinant plasmids, termed pTf100 and pTf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between E. coli and T. ferrooxidans. (unab.cl)
  • This detailed map demonstrates the power of high-resolution genetic mapping in the mouse as a means of determining locus order in a small chromosomal region and of providing an accurate framework for the construction of physical maps. (mpi.nl)
  • Evidence from restriction enzyme analysis and Southern blot DNA-DNA hybridization indicates that the three native plasmids share little consequence homology. (unab.cl)
  • Plasmids containing this gene, or a homologous gene. (addgene.org)
  • Initiating methionine, termination codon, poly A sites, and relevant restriction enzyme sites are indicated. (galeriaespacio48.com)
  • Students will be able to accurately draw a plasmid map based on the results of a restriction digest of a plasmid. (mtsac.edu)
  • Restriction maps are important when generating a construct for experimental use. (solvedlib.com)
  • Targeting plasmids for all recombinant cyclin viruses were generated by insertion of the cyclin cDNAs from p3X-FLAG plasmids between the and sites of the pGS-3700.cycFdR targeting plasmid. (galeriaespacio48.com)
  • You're probably aware of the two main types of restriction cloning: sticky-end and blunt-end cloning, but do you know the difference? (bitesizebio.com)
  • Patients entered the model insured under Medicaid and were treated under state-specific restrictions by Metavir fibrosis stage (base case) or all treated (all-patient strategy) with an approved all-oral regimen (ledipasvir/sofosbuvir [LDV/SOF] for 8 weeks or 12 weeks, depending on cirrhosis status, viral load, and state-specific LDV/SOF restrictions). (cdc.gov)
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
  • Assume that there is enough restriction enzyme that every possible restriction site on each molecule of DNA will be cut. (solvedlib.com)
  • You may not be able to create an account or request plasmids through this website until you upgrade your browser. (addgene.org)
  • Next, nine HER3-CTD truncation mutants were constructed to map minimal regions of transactivation potential using the Gal4 UAS-luciferase based system. (plos.org)
  • RESULTS: The sustained virologic response (SVR) rate of the current Medicaid LDV/SOF restriction strategy was 75.2% versus 95.9% if all LDV/SOF-eligible patients were treated under Medicaid. (cdc.gov)
  • Southern-blotting protocol was followed at the genotyping network except for the use of the plasmid pTBN12 as the probe. (cdc.gov)
  • Have questions about your order, deposit, or a plasmid? (addgene.org)
  • She subsequently worked as Assistant Professor in Residence in the Department of Microbiology from 1968 at the University of California, San Francisco and continued to study DNA restriction and modification with Herbert W Boyer. (wikipedia.org)
  • The current study aimed to map the putative transactivation domains (TADs) of the HER3 receptor. (plos.org)
  • Each plasmid expressed a 3x-FLAG-cyclin fusion protein at the expected size that was uniformly detected by the FLAG antibody and specifically detected by antibodies to each cyclin. (galeriaespacio48.com)