While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
Genes2
2013). Transposon mutagenesis identified chromosomal and plasmid genes essential for adaptation of the marine bacterium Dinoroseobacter shibae to anaerobic conditions. (tcdb.org)
this getting suggests that the 88 MDa plasmid offers afimbrial adhesin genes. (immune-source.com)
Proteins3
One method developed for making high ethanol yeast, for example, involves site-specific mutagenesis (see below) of regulatory proteins controlling a metabolic network for ethanol production, to make the yeast tolerate high levels of ethanol and glucose [2]. (i-sis.org.uk)
Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins. (genscript.com)
Site-directed mutagenesis of conserved T4P-specific residues in the PilC C-terminal domain yielded mutant proteins that supported wild-type pilus assembly but had a reduced capacity to support twitching motility, suggesting impairment of putative PilC-PilT interactions. (mcmaster.ca)
Bacterial3
The quick change kit provides the tools for changing the wild type gene carried on a bacterial plasmid. (i-sis.org.uk)
Rates and mechanisms of bacterial mutagenesis from maximum-depth sequencing. (cdc.gov)
Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules. (cdc.gov)
Mutations8
Site-directed mutagenesis (SDM) is a commonly used technique for introducing mutations into a gene of interest. (genengnews.com)
Here we describe the use of New England Biolabs' Gibson Assembly Master Mix in two different mutagenesis experiments: first, multiple mutations of the lacZ gene ( Figure 1 ) and second, the mutation of 5 of the 6 nucleotides at position 174-179 of eGFP from CTGACC to TTCTAT in order to change the amino acid sequence from LeuThr to PheTyr ( Figure 2 ). (genengnews.com)
Herein we have developed a widely applicable method for constructing unmarked mutations in this family of bacteria using a newly engineered temperature-sensitive plasmid, derived from an endogenous plasmid of M. haemolytica pD70. (usda.gov)
Two of the TS plasmids were shown by sequencing to possess unique single-base-pair mutations. (usda.gov)
These mutations were clustered within a 200-bp region of the presumed plasmid origin of replication. (usda.gov)
The mutant oligonucleotide is annealed to the wild type gene in the plasmid, which is amplified to produce plasmids carrying the gene with the specific mutations. (i-sis.org.uk)
The work 'Restriction digest screening facilitates efficient detection of site-directed mutations by CRISPR in Candida albicans' by Evans and colleagues describes a useful mutagenesis and screening strategy for the important fungal pathogen Candida albicans. (peerj.com)
Using our sophisticated gene synthesis and mutagenesis techniques, GenScript can introduce mutations at any site. (genscript.com)
Constructs1
The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis using our online quotation form . (genscript.com)
CDNA1
Cotransfection of the cells with a mixture of plasmid DNA consisting of TIF3 cDNA in the sense and in the antisense orientation resulted in significant inhibition of translation of the TIF3 protein. (cdc.gov)
Experiments1
NEB offers a selection of common and specialized DNAplasmids for use in cloning experiments and applications such as protein expression, gene expression, and cellular analysis. (neb.com)
Genome1
Mutagenesis with mobile genetic elements that insert into the genome offers a great scope for screening as these provide readily detected tags to identify insertion sites, and can potentially either activate or disrupt gene expression. (biomedcentral.com)
Residues1
By site-directed mutagenesis, we have identified several amino acid residues on cten and DLC-1 that are essential for this interaction. (rupress.org)
Efficient1
While mutagenesis at some loci is very efficient, others are more difficult, requiring extensive screening to identify successful mutants. (peerj.com)
Polymerase1
The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase and custom mutagenic primers to create substitutions, deletions and insertions for a wide variety of plasmids up to at least 14 kb in length. (neb.com)
Restriction4
To consistently transform H. somnus with TS plasmid, in vitro DNA methylation with commercially available HhaI methyltransferase was necessary to protect against the organism's restriction enzyme HsoI (recognition sequence 5'-GCGC-3') characterized herein. (usda.gov)
We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
The authors incorporation of restriction enzymes into CRISPR homologous replacement mutagenesis screens is related to previous work using restriction screening for site directed mutagenesis. (peerj.com)
The manuscript reports a clever incorporation of restriction enzymes into a CRISPR mutagenesis screening regime, as well as results of site-directed mutagenesis of the C. albicans UME6 transcription factor. (peerj.com)
Involves1
During this part of the course the student will do a project that involves specific or random mutagenesis techniques. (lu.se)
Deletion1
Deletion analysis on the wild-type pD70 plasmid replicon revealed that approximately 720 bp necessary for plasmid maintenance. (usda.gov)
Resistance1
Temperature-sensitive (TS) plasmids were generated through chemical mutagenesis of a derivative of the streptomycin resistance parent plasmid pD70, isolated from Mannheimia hemolytica serotype 1. (usda.gov)
Substitutions1
Learn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit. (neb.com)
The Q5 Site-Directed Mutagenesis Kit allows rapid site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. (neb.com)
Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)
Site-specific mutagenesis has been used to make yeast produce and tolerant high levels of ethanol [2, 7]. (i-sis.org.uk)
In the case of the high ethanol yeast, a commercial kit called the quick change site-specific mutagenesis kit was used. (i-sis.org.uk)
GenScript's site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. (genscript.com)
310 "A high efficiency method for site-directed mutagen -esis with any plasmid":Gene;1989 (Dec 7);84(1);p153-7.Average yield of mutants was 60% with simple and rapid techniques. (globalchange.com)
Techniques1
Existing techniques for SDM, such as whole plasmid SDM, while effective, are time consuming and prone to off-target mutation incorporation. (genengnews.com)
Products1
LABORATORY STUDIES directed mutagenesis, to distinguish plasmid-derived prod- ucts from authentic products in diagnostic applications. (cdc.gov)
Identify1
Annotate: automatically annotate common features, or manually annotate coding sequences and other features, design and annotate primers for PCR, sequencing, or mutagenesis, and identify open reading frames (ORFs) with a single mouse click. (cnet.com)
Analysis1
Three TS plasmids which failed to replicate at or above 42°C in M. hemolytica but which were fully functional below 31°C were selected for further analysis. (usda.gov)
Time2
Prices and turnaround times shown for Cat No. SC1441 apply to mutagenesis ordered at the same time as new gene synthesis orders. (genscript.com)
For mutagenesis from a template previously synthesized by GenScript (Cat No. SC1626), please Quote with your previous order number to request a quote for price and turnaround time. (genscript.com)
Data1
Share: export a plasmid map as an image, or export an annotated DNA sequence to GenBank format and freely share data with your colleagues or customers using the universally accessible SnapGene format. (cnet.com)
Base1
The third TS plasmid contained a unique base pair substitution and a second mutation that had been previously identified. (usda.gov)
Carry1
The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. (nih.gov)