• Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • The identification of the intracellular sGAG-interacting proteins could help to unravel these functions. (degruyter.com)
  • Insulin stimulates glucose transporter 4 (GLUT4) vesicle recruitment from its intracellular storage site to the plasma membrane. (tokushima-u.ac.jp)
  • Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present in all cell types that transfers electrons from intracellular substrates to extracellular acceptors maintaining redox homeostasis for a successful cell physiology [ 1 ]. (hindawi.com)
  • Maintaining the structure of intracellular membranes is essential for preserving normal cellular function. (phys.org)
  • [ 4 ] Protein hZIP4 transports zinc ions from the cell exterior or lumen of intracellular organelles into the cytoplasm, where it is available to other newly synthesized proteins. (medscape.com)
  • Glycine Plasma Membrane Transport Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This graph shows the total number of publications written about "Glycine Plasma Membrane Transport Proteins" by people in this website by year, and whether "Glycine Plasma Membrane Transport Proteins" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Glycine Plasma Membrane Transport Proteins" by people in Profiles. (childrensmercy.org)
  • Hence, the applications for imaging protein localization and dynamics require high accuracy, particularly at high spatio-temporal level. (biomedcentral.com)
  • Besides direct overlap of dual-color fluorescence signals, the co-localization analysis was carried out quantitatively in combination with the methodology for calculating puncta distance and protein proximity index. (biomedcentral.com)
  • Following up on the top hits obtained by proximity labeling, I study co-localization, protein-protein interaction, and genetics to expand our knowledge on the molecular networks that regulate stomatal development. (oeaw.ac.at)
  • Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
  • We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
  • Protein function is critically determined by subcellular localization, as organelles offer different chemical environments and interaction partners. (elifesciences.org)
  • In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)
  • We investigated control mechanisms that regulate the activity, localization, and stability of Wee1, especially the bud neck-localized protein kinase Hsl1 and its more distant paralogs (Gin4 and Kcc4), in particular their recruitment to septin filaments, which assemble at the presumptive site of cell division. (berkeley.edu)
  • The IZUMO1 protein and its receptor is the only sperm/oocyte interaction pair known to date that is necessary for gamete fusion in mice. (escholarship.org)
  • High resolution structures are available for individual receptors dimers, but less is known about receptor clusters that form in plasma membranes composed of many different RTKs with the potential to interact. (nature.com)
  • The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
  • Membrane receptor proteins serve as connection between the cell's internal and external environments. (phys.org)
  • Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
  • El receptor GlyA es sensible a la ESTRICNINA y se encuentra en la membrana postsináptica de las neuronas glicinérgicas inhibidoras. (bvsalud.org)
  • GlyA receptor is sensitive to STRYCHNINE and localized in the post-synaptic membrane of inhibitory glycinergic neurons. (bvsalud.org)
  • Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS. (ox.ac.uk)
  • Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)
  • Using an antibody to the extracellular domain of PMCA2 as a probe, we observed that PMCA2 diffuses laterally from the stereocilia membrane and is internalized at the apical cell border maintaining an estimated half-life of residency in the stereocilia of approximately 5-7 h. (elsevierpure.com)
  • The binding of bacteria or bacterial products to host proteins of tissue extracellular matrix may be a mechanism of tissue adherence. (nyu.edu)
  • Gelsolin is also an abundant extracellular protein that circulates in blood plasma, and Stossel established that it is a component of innate immunity that promotes host antimicrobial activity and prevents the potentially lethal dissemination of inflammation. (wikipedia.org)
  • Glycosaminoglycans (GAGs) are multifunctional polysaccharides of the extracellular matrix (ECM) responsible for ECM hydration and binding of cations and proteins due to their negative charge. (degruyter.com)
  • Proposed functions of PMRS include maintenance of redox state in proteins, stimulation of cell growth, reduction of lipid hydroperoxides, recycling of α -tocopherol, reduction of ferric ion prior to iron uptake by a transferring-independent pathway, and the maintenance of the extracellular concentration of ascorbic acid [ 2 - 6 ]. (hindawi.com)
  • Scientists have found that extracellular calcium mediates the activation of a membrane protein that waves the flag signaling cell death. (phys.org)
  • In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
  • In preparation for overwintering, they accumulate mixtures of different small molecules - most often sugars, amino acids and their derivatives, and also proteins, in their hemolymph. (cas.cz)
  • The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. (pubchase.com)
  • Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signaling and are suggested to be strongly associated with the actin cytoskeleton. (ox.ac.uk)
  • Attachment of YadA-positive and YadA-negative recombinant Yersinia enterocolitica strains to cartilage-derived human cellular fibronectin and human plasma fibronectin in the solid phase revealed that YadA mediates binding of yersiniae to cellular fibronectin in a saturable, concentration- dependent manner. (nyu.edu)
  • These observations provide evidence for the hypothesis that the binding region for YadA in cellular fibronectin is not present in plasma fibronectin. (nyu.edu)
  • This research led to the discovery of two important cellular proteins, filamin and gelsolin, that regulate the assembly of actin. (wikipedia.org)
  • By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
  • Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. (hindawi.com)
  • Curcumin modulates cellular activity through interacting with membrane-bound proteins and modulating signaling cascade activity by altering the fluidity of the membrane bilayer [ 17 ]. (hindawi.com)
  • Membrane proteins play a pivotal role in various cellular functions and are key targets for pharmaceutical interventions. (phys.org)
  • In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. (lu.se)
  • The present study characterizes an inhibitor of endomembrane protein trafficking, TE1 (trafficking and endocytosis inhibitor 1/TENin1) that reduces gravitropic root bending in Arabidopsis thaliana seedlings. (portlandpress.com)
  • I discovered a novel polarity protein in Arabidopsis thaliana that polarizes at distinct developmental stages in stomatal precursors. (oeaw.ac.at)
  • Identification of Candidate Ergosterol-Responsive Proteins Associated with the Plasma Membrane of Arabidopsis thaliana . (bvsalud.org)
  • They successfully detected the inward and outward confirmations of the symporter Sugar Transport Protein 10 (STP10) - a protein responsible for monosaccharide transport across cell membranes - at high resolutions (1.8 and 2.6 Å respectively) in Arabidopsis thaliana. (lu.se)
  • The G-protein on cholesterol-rich membrane microdomains mediates mucosal sensing of short- chain fatty acid and secretory response in rat colon. (nih.gov)
  • Sperm/oocyte membrane interaction in mammalian fertilization remains poorly understood. (escholarship.org)
  • The current study aims to find potential candidate proteins for sperm/oocyte plasma membrane interaction in a porcine model. (escholarship.org)
  • Antibody binding to its antigen would be predicted to reduce fertilization if that protein antigen was involved in gamete interaction. (escholarship.org)
  • In the IVF experiments, antibodies targeting oocyte proteins lipoprotein E (ApoE) and complement protein 6 (C6) significantly decreased the fertilization rate, suggesting that ApoE and C6 have a relevant role in the sperm/oocyte plasma membrane interaction. (escholarship.org)
  • To understand the molecular role of polarity proteins in plant development, it is important to characterize their interactome, including their protein-protein interaction partners. (oeaw.ac.at)
  • Part of heterotrimeric G-protein complex. (nih.gov)
  • The pheromone receptors have seven hydrophobic segments and are coupled to a heterotrimeric G protein. (berkeley.edu)
  • Here we use superresolution STED microscopy combined with fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics of fluorescent lipid analogues and GPI-anchored proteins (GPI-APs) in the live-cell plasma membrane and in actin cytoskeleton-free, cell-derived giant plasma membrane vesicles (GPMVs). (ox.ac.uk)
  • Our data underline the crucial role of the actin cortex in maintaining hindered diffusion modes of many but not all of the membrane molecules and highlight a powerful experimental approach to decipher specific influences on molecular plasma membrane dynamics. (ox.ac.uk)
  • This rapid turnover of a major stereocilia membrane protein matches the previously described rapid turnover of proteins of the stereocilia actin core, further demonstrating that these organelles undergo rapid continuous renewal. (elsevierpure.com)
  • We find that these receptors are intermixed nonhomogenously on the plasma membrane. (nature.com)
  • Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of serotonergic neurons. (musc.edu)
  • Second, the eluted proteins were identified by two different LC/MS-MS approaches using bioinformatic tools. (escholarship.org)
  • notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. (nature.com)
  • Biological membranes consist of a phospholipid bilayer and a variety of proteins that accomplish vital biological functions. (phys.org)
  • Ypk1, in turn, regulates (via its phosphorylation of multiple substrates) maintenance of sphingolipid and glycerolipid homeostasis and bilayer lipid organization in the plasma membrane. (berkeley.edu)
  • B) proteins can span the bilayer. (flashcardmachine.com)
  • Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes. (lu.se)
  • Our results suggest that the SHOU4 proteins regulate cellulose synthesis in plants by influencing the trafficking of CESA complexes to the cell surface. (nih.gov)
  • Over-expression of wild-type CSP1 led to attenuated insulin-stimulated glucose uptake without any change in GLUT4 content in the plasma membrane, rather it inhibits docking by blocking the association of VAMP2 with syntaxin 4. (tokushima-u.ac.jp)
  • [ 3 ] This protein controls zinc uptake across the plasma membrane of various cell types, including the intestine. (medscape.com)
  • Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. (lu.se)
  • Third, the biological role of selected candidate proteins was evaluated in in vitro fertilization (IVF) trials employing commercially available antibodies to selected proteins. (escholarship.org)
  • Based on in vitro assays and theory, insect physiologists hypothesized that these molecules protect insect enzymes and biological membranes against loss of. (cas.cz)
  • The aquaporins (AQPs) form a family of integral membrane proteins that facilitate the movement of water across biological membrane by osmosis, as well as facilitating the diffusion of small polar solutes. (lu.se)
  • These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. (pubchase.com)
  • Deficiency in plasma membrane calcium ATPase isoform 2 increases susceptibility to noise -induced hearing loss in mice. (cdc.gov)
  • Mice homozygous for a null mutation in the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) gene are deaf (Kozel et al. (cdc.gov)
  • Recent evidence indicates that SLP-2 is involved in the organization of cardiolipin-enriched microdomains in mitochondrial membranes and the regulation of mitochondrial biogenesis and function. (ox.ac.uk)
  • Based on these results, we propose that SLP-2 facilitates the compartmentalization not only of mitochondrial membranes but also of the plasma membrane into functional microdomains. (ox.ac.uk)
  • The nucleus is surrounded by a membrane called the nuclear envelope, which protects the DNA and separates the nucleus from the rest of the cell. (medlineplus.gov)
  • protein trafficking. (nih.gov)
  • During development, protein trafficking and storage require an extensive reorganization of the endomembrane system. (nature.com)
  • Depending on the protein trafficking, PB composition is affected, thereby influencing the qualitative output. (nature.com)
  • Rapid turnover of stereocilia membrane proteins: evidence from the trafficking and mobility of plasma membrane Ca(2+)-ATPase 2. (elsevierpure.com)
  • We studied the spatial distribution, mobility, and trafficking of plasma membrane Ca2+ATPase-2 (PMCA2), a protein enriched in the hair cell apical membrane and essential for hair cell function. (elsevierpure.com)
  • Dive into the research topics of 'Rapid turnover of stereocilia membrane proteins: evidence from the trafficking and mobility of plasma membrane Ca(2+)-ATPase 2. (elsevierpure.com)
  • The short-term protein trafficking inhibition and long-term effect on plant growth and survival caused by TE1 were fully reversible upon drug washout. (portlandpress.com)
  • Cysteine string protein 1 (CSP1) is a SNARE-binding protein involved in the vesicular trafficking of neurotransmitters and other exocytic processes. (tokushima-u.ac.jp)
  • However, recent studies have suggested that targetting the trafficking of AQP proteins to the plasma. (lu.se)
  • However, recent studies have suggested that targetting the trafficking of AQP proteins to the plasma membrane is a viable alternative drug target to direct inhibition of the water-conducting pore. (lu.se)
  • By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. (nature.com)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • First, sperm and oocyte plasma membrane proteins with affinity for proteins of the plasma membrane of their partner gamete were separated by affinity chromatography. (escholarship.org)
  • Approximately 100 oocyte plasma membrane proteins with affinity for the sperm, and 100 sperm plasma membrane proteins with affinity for the oocyte were identified with the two MS approaches. (escholarship.org)
  • Some of these proteins were already suspected in involvement in gamete recognition and some met the threshold for affinity compared with nonspecific controls but did not exhibit a particularly strong affinity for the complementary gamete. (escholarship.org)
  • This study complements the knowledge from previous analyses on gamete interactions by using a molecular-affinity-driven process to identify sperm/oocyte plasma membrane interacting components. (escholarship.org)
  • Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)
  • We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)
  • Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. (nature.com)
  • Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
  • Membrane enzymes produce a variety of substances essential for cell function. (phys.org)
  • A symporter is an integral membrane protein that is involved in the transport of two different molecules across the cell membrane in the same direction. (lu.se)
  • We provided experimental data for the applications of Variable-Angle Epifluorescence Microscopy (VAEM) in dissecting protein dynamics in plant cells. (biomedcentral.com)
  • Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5's active and structural roles. (pubchase.com)
  • Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
  • Structural proteins are attached to microfilaments in the cytoskeleton which ensures stability of the cell. (phys.org)
  • In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)
  • GSH is a hydrophilic antioxidant with nucleophilic thiol group and has been reported to participate in regulation of gene expression, protein synthesis, cell proliferation, signal transduction, cytokine production, apoptosis, immune response, and protein glutathionylation [ 11 ]. (hindawi.com)
  • These strategies involve rearrangements at the molecular level starting from transcription, regulation of mRNA processing, translation, and protein modification or its turnover. (intechopen.com)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)
  • once we know what compartment a protein is in, it is easier to narrow down what it might be doing. (elifesciences.org)
  • We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
  • Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
  • We investigated interactions of the plasmid-encoded outer membrane protein YadA, which confers pathogenic functions on enteropathogenic yersiniae, with fibronectin. (nyu.edu)
  • The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
  • Finally, transport proteins play an important role in the maintenance of concentrations of ions. (phys.org)
  • Moreover, endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. (nature.com)
  • Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. (hindawi.com)
  • Sex hormone-binding globulin (SHBG) is the blood transport protein for testosterone and estradiol. (cdc.gov)
  • Molecular Membrane Biology 2013, 30 (3):246-260. (lu.se)
  • The present study presented experimental evidence on illustrating the use of VAEM in tracking and dissecting protein dynamics, dissecting endosomal dynamics, cell structure assembly along with membrane microdomain and protein motility in intact plant cells. (biomedcentral.com)
  • Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? (pubchase.com)
  • Using a green fluorescent protein tag, we observed that PMCA2 is rapidly delivered to the apical cell border from where it diffuses to the entire stereocilia surface. (elsevierpure.com)
  • Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. (lu.se)
  • Auxin gradients are established and maintained by polarized distribution of auxin transporters that undergo constitutive endocytic recycling from the PM (plasma membrane) and are essential for the gravitropic response in plants. (portlandpress.com)
  • Although the major pool of SLP-2 is associated with mitochondria, we show here that there is an additional pool of SLP-2 associated with the plasma membrane of T cells. (ox.ac.uk)
  • Both plasma membrane-associated and mitochondria-associated pools of SLP-2 coalesce at the immunological synapse (IS) upon T cell activation. (ox.ac.uk)
  • Our data also suggest that there may be a net exchange of membrane material between mitochondria and plasma membrane, explaining the presence of some mitochondrial proteins in the plasma membrane. (ox.ac.uk)
  • Seedling morphology, H+-ATPase activity and content, 14-3-3 protein content, binding to PM and phosphorylation, gene expression, and ROS quantification were assessed in maize (Zea mays) dark-grown seedlings. (unito.it)