Plasma membrane potentials. Medical search. Frequent questions

The current study aims to find potential candidate proteins for sperm/oocyte plasma membrane interaction in a porcine model. (escholarship.org)
First, sperm and oocyte plasma membrane proteins with affinity for proteins of the plasma membrane of their partner gamete were separated by affinity chromatography. (escholarship.org)
Approximately 100 oocyte plasma membrane proteins with affinity for the sperm, and 100 sperm plasma membrane proteins with affinity for the oocyte were identified with the two MS approaches. (escholarship.org)
In addition, a library of porcine oocyte membrane proteins was generated containing more than 200 proteins. (escholarship.org)
In the IVF experiments, antibodies targeting oocyte proteins lipoprotein E (ApoE) and complement protein 6 (C6) significantly decreased the fertilization rate, suggesting that ApoE and C6 have a relevant role in the sperm/oocyte plasma membrane interaction. (escholarship.org)
Curcumin modulates cellular activity through interacting with membrane-bound proteins and modulating signaling cascade activity by altering the fluidity of the membrane bilayer [ 17 ]. (hindawi.com)
Ras proteins are attached by a fatty tail to the inner surface of the cell membrane. (elifesciences.org)
The effect of cholesterol stereoisomers, in particular, has been established, identifying a direct link with the activity of specific membrane proteins. (bath.ac.uk)
utilizing this photoreactivity advantageously, [3H]LSD was used to photolabel membrane proteins. (erowid.org)
Sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis of [3H]LSD labeled membranes from F. hepatica showed two proteins which were selectively labeled by the photoreactive [3H]LSD. (erowid.org)
These proteins act as channels and allow ions to move across cell membranes. (elifesciences.org)
Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
Chloride channels are various group of proteins that control fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, regulation of intracellular pH, and maintenance of cell volume. (prospecbio.com)
The aquaporins (AQPs) form a family of integral membrane proteins that facilitate the movement of water across biological membrane by osmosis, as well as facilitating the diffusion of small polar solutes. (lu.se)
However, recent studies have suggested that targetting the trafficking of AQP proteins to the plasma. (lu.se)
However, recent studies have suggested that targetting the trafficking of AQP proteins to the plasma membrane is a viable alternative drug target to direct inhibition of the water-conducting pore. (lu.se)

The sodium channels open rapidly in response to initial depolarization of the axon plasma membrane, allowing sodium ions (Na + ) to flood in. (encyclopedia.com)
Depolarization also triggers less rapid opening of the potassium channels, which permits outflow of potassium ions (K + ), thus acting to restore the membrane potential to its resting state. (encyclopedia.com)
Two-pore domain potassium (K(+)) channels (K2P channels) control the negative resting potential of eukaryotic cells and regulate cell excitability by conducting K(+) ions across the plasma membrane. (rcsb.org)
Biologists have taken great pains over the years to document the minute workings of the openings in cell membranes that allow hydrogen, sodium, calcium and other ions to make their way inside across the barrier that envelops the cell and its contents. (scientificamerican.com)
These mechanisms are essentially the opening and closing of certain pores that allow certain ions to pass freely across the cell membrane. (scientificamerican.com)
They span the membrane of cells, allowing the selective permeation of K + ions from one side of the membrane to the other, usually from the inside of the cell to the outside. (ersjournals.com)

The sodium channel protein has positively charged voltage-sensing regions, which move towards negative charges on the outer surface of the membrane when the latter becomes depolarized. (encyclopedia.com)
Transient receptor potential canonical 6 (TRPC6) is a cation selective, DAG-regulated, Ca 2+ -permeable channel activated by the agonists of G q -protein-coupled heptahelical receptors. (mdpi.com)
When the researchers mutated the C-terminal domain of VP40, the protein was much less effective at binding to and bending membranes and forming virus-like particles that could escape from host cells. (eurekalert.org)
The Ebola Virus Matrix Protein Deeply Penetrates the Plasma Membrane: An Important Step in Viral Egress. (eurekalert.org)
The presence of plasma membrane multidrug resistance protein P-glycoprotein (P-gp) is favorable for tumor cells to elicit chemotherapeutic resistance. (jcancer.org)
Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)

In other words, what makes cells with excitable membranes so unusual is their response to electrostatic disturbances of homeostasis (slight acidification of the normally alkaline cellular interior) following external stimulation. (scientificamerican.com)
This gene subfamily is constitutively active at physiological resting membrane potentials in excitable cells, including smooth muscle cells, and has been particularly linked to the human pulmonary circulation. (ersjournals.com)

abstract = "Cholesterol is a major constituent of the plasma membrane in higher order eukaryotes. (bath.ac.uk)

voltage-gated ion channel Any ion channel that opens and closes in response to changes in electrical potential across the cell membrane in which the channel is situated. (encyclopedia.com)
Two types are especially important in transmitting action potentials along axons: voltage-gated sodium channels and voltage-gated potassium channels. (encyclopedia.com)
As a consequence, the attached plasma membrane is depolarised and voltage-gated ion channels are activated, triggering cellular action potentials. (tugraz.at)

Nevertheless, iron excess has the potential to catalyze the formation of cell-damaging reactive oxygen species ( Halliwell and Gutteridge, 1984 ). (frontiersin.org)

Zeta potential measurements were also performed, giving potentially useful information about the biological functionality of exosomes. (anton-paar.com)
From a biological perspective, we suggest that the lowest-level candidate mechanism is membrane "excitability:" the unusual capability of certain types of living cells to sense and respond to stimuli within several milliseconds. (scientificamerican.com)
Rather, the dynamics of the exchange of materials across biological membranes differ remarkably among cell types. (scientificamerican.com)
I have analyzed the contradictory items of the FRTA, outlining a new, comprehensive interpretation of the possible biological role of oxygen free radicals in the living state, cell differentiation and aging, called the membrane hypothesis of aging (MHA). (antiaging-systems.com)
In addition to biological tissues, these systems have potential applications for the assessment of bioengineered tissues, biomaterials with fine structures, or some engineering materials. (who.int)

We characterized a glucose dependent hyperpolarization of the mitochondrial membrane potential (Δψ m ) in the majority of neurons after transient glutamate excitation. (jneurosci.org)
The largest and significant increases occurred in the basilar membrane, spiral ganglion neurons and stria vascularis of the cochlea. (cdc.gov)

Exosomes are nanometer-range vesicles originating from the fusion of endosomes with the plasma membrane of eukaryotic cells. (anton-paar.com)

Exosomes are small membrane vesicles of endocytic origin that are released into the extracellular compartment upon fusion of multivesicular bodies (MVB) with the plasma membrane. (dovepress.com)
To address this question, Stahelin and his team made vesicles designed to mimic the plasma membrane of host cells and exposed these vesicles to VP40. (eurekalert.org)

Published 24 January 2019 We explored plasma extracellular vesicle immunoprofiling as a tool for noninvasive diagnosis of glioma. (lu.se)

2016). AA also takes part in the regeneration of α-tocopherol (vitamin E) in photosynthetic membranes and participates in chloroplast metabolism as a cofactor for violaxanthin de-epoxidase, an enzyme involved in xanthophyll cycle-mediated photoprotection (Smirnoff and Wheeler, 2000). (deepdyve.com)
Published 20 April 2016 Hypoxia modulates global membrane proteome turnover in cancer cells, providing opportunities for tumor specific drug delivery. (lu.se)

Ultrasound elastomicroscopy using water jet and osmosis loading: potentials for assessment for articular cartilage. (who.int)

VP40 also caused the vesicle membranes to bend into the shape of the Ebola virus, paving the way for its escape. (eurekalert.org)

In addition, PMRS also regulates physiological functions like cell metabolism, cellular growth, activity of ion channels, and cell death against changes in redox potential [ 7 , 8 ]. (hindawi.com)
While the in vivo physiological relevance of exosomes is still largely unclear, their potential as drug delivery system has long been recognized. (anton-paar.com)

Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. (hindawi.com)
In light of the fact that all living cells have enveloping membranes and exchange materials with their external worlds, it is unlikely that metabolic activity, biochemical homeostasis [keeping cellular systems in balance], or the mere presence of a boundary between the cellular self and the external world alone is sufficient to explain the origins of mind. (scientificamerican.com)
Dr. Jacobs described Vaccinia viruses deficient in E3L, a regulator of the cellular antiviral response and noted their potential for the production of improved vaccines. (cdc.gov)

Normally, vasodilatation within these arteries occurs through activation of large-conductance, calcium-activated potassium channels on the plasma membrane by nearby localised calcium-release events - referred to calcium sparks - from the sarcoplasmic reticulum (SR) of vascular smooth muscle cells. (hospitalhealthcare.com)

This study complements the knowledge from previous analyses on gamete interactions by using a molecular-affinity-driven process to identify sperm/oocyte plasma membrane interacting components. (escholarship.org)
Currently, we are trying to find small molecules that can inhibit VP40 interactions with the plasma membrane," Stahelin says. (eurekalert.org)
Based on these simulations, an atomic-level description of the stereospecific cholesterol-phospholipid interactions is provided, establishing a potential mechanism for the perturbation of membrane properties, specifically the membrane dipole potential. (bath.ac.uk)
Oakes, V & Domene, C 2018, ' Stereospecific Interactions of Cholesterol in a Model Cell Membrane: Implications for the Membrane Dipole Potential ', Journal of Membrane Biology , vol. 251, no. 3, pp. 507-519. (bath.ac.uk)

KRas G12D exhibits an immobile state in ~70 nm domains, each embedded in a larger domain (~200 nm) that confers intermediate mobility, while the rest of the membrane supports fast diffusion. (elifesciences.org)

Specifically, the ONC coating protected against a silica-induced inflammatory response, but induced macrophage plasma and phagosome membrane damage, SAEC membrane damage, and robust stimulation of in vitro/in vivo LF reticular fiber and collagen production. (cdc.gov)

Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. (hindawi.com)
Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present in all cell types that transfers electrons from intracellular substrates to extracellular acceptors maintaining redox homeostasis for a successful cell physiology [ 1 ]. (hindawi.com)
The use of the cationic, dye thioflavin T (ThT), to estimate the electric plasma membrane potential difference ( PMP ) via the fluorescence changes and to obtain its actual values from the accumulation of the dye , considering important correction factors by its binding to the internal components of the cell , was described previously for baker's yeast . (bvsalud.org)
Potential for corneal endothelium cell loss. (nih.gov)
There is an increased potential for developing corneal edema in patients with low endothelial cell counts. (nih.gov)
From there, the cell constantly removed Ras molecules from these membrane domains and returned them back to their 'fast' diffusing state. (elifesciences.org)
The effect of cholesterol on the structure and organisation of cell membranes has been studied extensively by both experimental and computational means. (bath.ac.uk)
The attached part of the cell membrane is separated from the top layer of the photocap by a thin layer of electrolyte, and the free part is in contact with the bath. (tugraz.at)

Sperm/oocyte membrane interaction in mammalian fertilization remains poorly understood. (escholarship.org)
Here we review the literature on the trafficking of mammalian AQPs with a view to highlighting potential new drug targets for a variety of conditions associated with disrupted water and solute homeostasis. (lu.se)

Due to these membrane alterations, an accumulation of dry mass (i.e., a decrease of the intracellular water content) takes place in the intracellular space. (antiaging-systems.com)

When the membrane potential is considered, PCFT generates substantial transmembrane electrochemical-potential gradients at extracellular pH levels relevant to the tumor microenvironment. (aspetjournals.org)

They regulate the excitability of cells and contribute to their resting membrane potential [ 1 , 2 ]. (ersjournals.com)

Also, they can clump together or agglomerate, which can affect their potential for settling in the lungs if inhaled, their ability to penetrate the body's membranes and consequently move from the lungs to other organs, and their interaction with cells and tissue. (cdc.gov)

We examined the bioelectric properties (resting potential gradients in the epidermis) of Xenopus laevis froglets undergoing hindlimb amputation and observed that the contralateral (undamaged) limb exhibits apparent depolarization signals immediately after the opposite hindlimb is amputated. (biologists.com)
The alterations are due in part to free radical induced molecular damage, and also to the 'residual heat' formed during each depolarization of the resting potential. (antiaging-systems.com)

Journal of Membrane Biology , 251 (3), 507-519. (bath.ac.uk)
Molecular Membrane Biology 2013, 30 (3):246-260. (lu.se)

Recent studies show that modulation of TASK-1 channels, either directly or indirectly by targeting their regulatory mechanisms, has the potential to control pulmonary arterial tone in humans. (ersjournals.com)
CLIC1 inserts into membranes and forms chloride ion channels. (prospecbio.com)
In addition we have recently started to explore the structure and molecular function of pain receptors from animals, i.e. transient receptor potential (TRP) ion channels, and their activation by temperature and various ligands. (lu.se)

This rather efficient photoreaction yields a variety of products which have very low affinity for LSD-hinding sites on plasma membranes from Fasciola hepatica. (erowid.org)
Since this photoreaction may be elicited by normal white fluorescent lighting in the laboratory, it represents a potential source of error in determining the binding affinity of LSD. (erowid.org)

O 2 and N 2 gases permeation through the 10-minute plasma treated membranes tend to increase. (hindawi.com)
For the CO 2 /CH 4 separation, the hydrophilic property of the membrane surface has an influence on the CO 2 permeation rate. (hindawi.com)
Therefore, the permeation rate of CO 2 was enhanced by increasing the hydrophilic property of the membrane surface [ 3 , 4 ]. (hindawi.com)
However, the increase of gas permeation rate depends on many parameters like kinetic diameter, quadrupole moment, and polarizability of gas, as well as the polarity of polymeric membrane surfaces. (hindawi.com)

In order to increase the permeability of gas, hydrophilic properties, selective layer thickness, and porosity of the membranes have to be improved. (hindawi.com)

Sestamibi is taken up by tissues with large numbers of mitochondria and negative plasma membrane potentials. (wikipedia.org)
HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues. (lu.se)
These guidelines address the use of tests for the hepatitis B and C viruses to screen donations of blood and plasma collected for transfusion or further manufacture into injectable products, as well as to screen donors of organs, tissues, and semen. (cdc.gov)

Liu C, Pan Y, Li Q, Zhang Y. Bioinformatics analysis identified shared differentially expressed genes as potential biomarkers for Hashimoto's thyroiditis-related papillary thyroid cancer. (medsci.org)

Here, using single particle tracking with photoactivated localization microscopy (spt-PALM) and detailed trajectory analysis, we show that distinct membrane domains dictate KRas G12D (an active KRas mutant) diffusion and trafficking in U2OS cells. (elifesciences.org)
Our results reveal how membrane organization dictates membrane diffusion and trafficking of Ras and offer new insight into the spatial regulation of Ras signaling. (elifesciences.org)

High resolution structures are available for individual receptors dimers, but less is known about receptor clusters that form in plasma membranes composed of many different RTKs with the potential to interact. (nature.com)
Effects of direct current glow discharge plasma using argon as working gas on morphological structures and gas separation properties of membranes were studied. (hindawi.com)
It was also shown that the dosage of PEG and plasma treatment affected the morphological structures and mechanical and gas separation properties. (hindawi.com)

Alteration of membrane characteristics were analyzed by various techniques like contact angle, scanning electron microscope, Fourier transform infrared spectroscopy, and dynamic mechanical thermal analysis. (hindawi.com)

Exosomes are intensively studied as potential drug delivery vehicles. (anton-paar.com)

Most glow discharge plasmas used for these aims are generated from radio frequency (13.56 MHz) and microwave (2.45 GHz) power sources. (hindawi.com)

Polysulfone/polyethylene glycol (PSF/PEG) membranes were prepared by dry/wet phase inversion method. (hindawi.com)
Typically, the permeability of asymmetric polymeric gas separation membranes prepared by phase inversion techniques is quite low and unsuitable for practical use. (hindawi.com)
In 1998, Kim and Lee [ 5 ] studied the effect of PEG on membrane formation by phase inversion. (hindawi.com)

Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)

For a long time, estimations and actual measurements of the electric plasma membrane potential (PMP) in whole yeast cells have been the subject of studies by several groups without reliable results. (intechopen.com)

In this review, we discuss the changes in irradiated cancer cells and immune cells in the TME under different RT regimens and describe existing and potential molecules that could be targeted to improve the therapeutic effects of RT. (nature.com)
It has been suggested that to prevent these signals from being accidently activated, Ras molecules must group together at specialized sites within the membrane before passing on their message. (elifesciences.org)
This revealed that Ras molecules quickly diffuse along the inside of the membrane until they arrive at certain locations that cause them to halt. (elifesciences.org)
However, computer models consisting of just the 'fast' and 'immobile' state could not correctly re-capture the way Ras molecules moved along the membrane. (elifesciences.org)
The map showed regions in the membrane where the Ras molecules had stopped and possibly clustered together. (elifesciences.org)

Plasma surface modification is one of the processes with high potential that can be used to raise the utility of membrane materials in diverse technical applications. (hindawi.com)
Generally, etching and deposition processes occur at the same time when the polymeric membranes are treated with the discharge plasma from non-polymer forming gases [ 1 ]. (hindawi.com)
Nevertheless, these processes are depended on the initial plasma conditions. (hindawi.com)
The MHA attributes a leading role in differentiation and aging processes to the plasma membrane, undergoing inevitable, continuous alterations during the life. (antiaging-systems.com)

Little research is available on how the Ebola virus buds from the plasma membrane of human cells," says senior study author Robert Stahelin of Indiana University School of Medicine. (eurekalert.org)
Past studies have shown that a part of VP40 called the C-terminal domain penetrates the plasma membrane surrounding host cells. (eurekalert.org)
But until now, it was not known exactly how VP40 binds to the plasma membrane to allow the virus to escape host cells. (eurekalert.org)
It gives a full rundown of the current NP-based vaccines, their potential as adjuvants, and the ways they can be delivered to cells. (researchgate.net)

This effort could lead to the discovery of potential drug candidates that could form the basis of much-needed therapies for this deadly virus. (eurekalert.org)

Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). (rndsystems.com)

CLIC1 displays both nuclear and plasma membrane chloride ion channel activity. (prospecbio.com)

By shedding light on this process, our study will help us to identify potential drug candidates that could interfere with this step in the viral life cycle. (eurekalert.org)

The Plasma Membrane Electric Potential in Yeast: Probes, Results, Problems, and Solutions: A New Application of an Old Dye? (intechopen.com)
Results showed that hydrophilic and gas separation properties of PSF/PEG membranes increased by plasma surface modification. (hindawi.com)
On the basis of these results, CPH could be considered as a potential anti-aging drug. (antiaging-systems.com)
A potential therapeutic target underlying the cause of reduced cerebral blood flow to the brain that results in vascular dementia has been recently discovered by researchers at the University of Manchester. (hospitalhealthcare.com)

In summary, incineration status and presence of organic coating influences the potential mode of action of the pulmonary response. (cdc.gov)
Hearing function was assessed using distortion product otoacoustic emissions (DPOAE), auditory brainstem responses (ABR) and the compound action potential (CAP). (cdc.gov)

Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes. (lu.se)

Data from EPINet revealed that 68.5% settings and who has the potential for expo- of injuries were linked to hollow-bore sure to infectious materials including body needles (syringes, butterfly needles, phle- substances, contaminated medical supplies botomy needles, needles on IV lines and and equipment, contaminated environmen- blood gas syringes) [ 1 ]. (who.int)

Addition of organic or inorganic particle as a third component of the casting solution is one of the most important chemical techniques, while the plasma surface modification is a physical (plasma treatment) and physicochemical (plasma polymerization and grafting) technique. (hindawi.com)
The Food and Drug Administration (FDA) currently requires that all donations of whole blood and transfusable components as well as plasma for fractionation into injectable derivatives be subjected to a serologic test for syphilis, hepatitis B surface antigen (HBsAg), and antibody to the human immunodeficiency virus (anti-HIV). (cdc.gov)

In Experiment 1, we treated rats with 10 g/L MnCl2 in drinking water for 60 or 90 days and used inductively coupled plasma mass spectrometry to quantify the increase in Mn levels in three regions of the cochlea and three brain regions. (cdc.gov)

Treatment and prognosis vary by the etiology and potential reversibility of the disorder at the time of diagnosis. (msdmanuals.com)

Anatomy53

Organisms16

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes47

Disciplines and Occupations3

Technology, Industry, Agriculture5

Information Science3

Health Care1

Proteins16

Ions6

Protein6

Excitable2

Abstract1

Voltage-gated3

Reactive oxygen1

Biological5

Neurons2

Eukaryotic cells1

Vesicles2

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Osmosis1

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Physiological2

Cellular3

Potassium1

Interactions4

Confers1

Stimulation1

Cell8

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Intracellular1

Tumor1

Excitability1

Interaction1

Depolarization2

Biology2

Channels3

Affinity2

Permeation4

Selective1

Tissues3

Biomarkers1

Diffusion2

Structures3

Electron1

Exosomes1

Aims1

Phase3

Glucose1

Measurements1

Molecules5

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Cells4

Form1

Found1

Channel1

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Results4

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Transport1

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Water1

Diagnosis1