Plasma growth applications cells cultured. Medical search. Frequent questions

The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Therefore, the amount of cholesterol available in vitro may be insufficient, particularly during cell proliferation, repair and maintenance of organ-specific functions (e.g. in cells of the nervous system during neurite extension, synaptogenesis, and myelination). (forschung3r.ch)
Both in-vitro and in-vivo studies revealed that cold plasmas selectively kill cancer cells. (nature.com)
To evaluate their in vitro biological responses, we conducted experiments involving porcine coronary artery endothelial cells (PCAECs) and smooth muscle cells (PCASMCs). (programmaster.org)
A method for stimulation of in-vitro cell cultures electrical activity with visible light, based on the use of organic semiconducting polymers is described. (jove.com)
Our method is described here for photostimulation of in vitro cell culture. (jove.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Cold atmospheric plasma (CAP), a highly reactive (partially) ionized physical state containing a dose controlled mixture of biologically-active agents has been shown to exert anticancer effects in vitro and in vivo ( 6 - 9 ). (iiarjournals.org)
The purpose of this kit is the in-vitro quantitative determination of human IL-2 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate). (biosensis.com)
In contemporary usage, "tissue culture" usually relates to the extension of cells from a tissue of a multicellular organism in vitro. (microbiologynote.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. (gentaur.com)
Our data showed that CBP is equivalent to M FBS for culturing placental MSCs with respect to the phenotype, proliferation rate and differentiation to various lineages. (brjnmims.org)
Background/Aim: Cold atmospheric plasma (CAP) attenuates tumor cell proliferation and induces apoptosis in various cell lines. (iiarjournals.org)
The aim of the study was to analyse the effects of different CAP sources and application times on osteosarcoma (OS) cells and non-malignant fibroblast cell proliferation. (iiarjournals.org)
Cell proliferation was determined by viable cell count at different time points after treatment. (iiarjournals.org)
Conclusion: CAP treatment attenuates cell proliferation of OS cancer cells and fibroblasts in a treatment time-dependent manner, whereby U2-OS cells appeared more sensitive to CAP treatment as 3T3 fibroblasts after 10 sec of treatment. (iiarjournals.org)
Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well. (accela.eu)
Porous scaffolds for cell cultured meat are currently limited in the food-grade material requirements, the cell adhesion, proliferation, and differentiation capacities, and the ignored appearance design. (bvsalud.org)
Due to the abundant tripeptide presence and well-aligned porous structures, the scaffold could not only provide sites for cell adhesion and proliferation, but also promote the oriented growth and differentiation of cells. (bvsalud.org)

Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit. (anticorps-enligne.fr)
The panel has been validated for use on cell culture supernatant, serum, and plasma samples. (biolegend.com)
For the quantification of Interleukin-2 (IL-2) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate). (biosensis.com)

5. What is the best ECM protein to study stem cell differentiation? (sigmaaldrich.com)
Stem cells express varied integrins on the surface during different stages of differentiation. (sigmaaldrich.com)
Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
Description: Quantitativecompetitive ELISA kit for measuring Rat growth differentiation factor 15(GDF15) in samples from serum, plasma, tissue homogenates. (scalegen.com)
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. (gentaur.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
The ability to artificially simulate the 'mechanical' niche, broadly termed as Extracellular Matrix (ECM), of the bone marrow determines success for stem cell growth, architectural organization and differentiation viz. (brjnmims.org)
miR-203 drives breast cancer cell differentiation. (medscape.com)
The up-regulation of myogenic related genes, synthesis of myogenic related proteins and formation of matured myotubes furtherly proved the differentiation of cells on aligned scaffold. (bvsalud.org)
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)

Tissue engineering performs the culture of cells on scaffolds, aiming at the restoration of damaged tissue. (springer.com)
Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
We proposed programmable scaffolds specially tailored for cell cultured meat. (bvsalud.org)
Meanwhile, patterned scaffolds were achievable as well with the help of mold-assisted ice templating, which would improve the people's interest, recognition, and acceptance of the tailored cell cultured meat. (bvsalud.org)
These characteristics indicate great application prospects of the proposed programmable scaffolds in cell cultured meat. (bvsalud.org)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)

Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero cell culture, in a one case report study. (springer.com)
Platelet-rich plasma: the choice of activation method affects the release of bioactive molecules. (springer.com)
Use of autologous platelet-rich plasma in the treatment of dormant corneal ulcers. (medscape.com)
Subsequent regenerative dental procedures include the development of guided tissue or bone regeneration (GTR, GBR) procedures and distraction osteogenesis 2 the application of platelet rich plasma (PRP) for bone augmentation 17 . (bvsalud.org)

The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
Hyphal tip polarity was altered when V. longisporum was confronted with pseudomonads in narrow interaction channels, resulting in a curly morphology instead of straight hyphal tip growth. (frontiersin.org)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
The efficiency of the cultures was analyzed by growth curve, morphology, phenotype and functionality. (brjnmims.org)
Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. (accela.eu)

Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
To investigate the cytoxicity of Ecasol using cultured keratinocyte monolayers and reconstituted human oral epithelial (RHE) tissue and to extend the study of Ecasol's effectiveness in maintaining the microbiological quality of DUWL output water. (researchgate.net)

Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Neonatal tissues, cord and placenta, are explored as alternate sources of mesenchymal stem cells (MSCs) for their therapeutic applications. (brjnmims.org)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Ehrenfest DMD, Del Corso M, Diss A, Mouhyi J, Charrier J-B. Three-dimensional architecture and cell composition of a Choukroun's platelet-rich fibrin clot and membrane. (springer.com)
The tech allows for selective cultivation of egg cells-the amnion, yolk sac, and plasma membrane-"amplify" the potency of the nutritional elements in eggs. (greenqueen.com.hk)
In the organism, liposoluble nutrients such as cholesterol, fatty acids and lipophilic vitamins reach the cells by means of specific apolipoproteins which form complexes with the lipids, the so-called lipoproteins (LPs), and which recognize specific membrane receptors expressed by the cells. (forschung3r.ch)
It is an antifungal polyene macrolide that binds to ergosterol and blocks fungal growth, without changing the permeability of the plasma membrane. (toku-e.com)
The overall goal of this method is to control the cell membrane potential upon stimulation by visible light pulses. (jove.com)
Furthermore, vacuolar-type H+-ATPase (V-ATPase) and H+/Cl- exchange transporter 7 (CLC-7) were detected on the surface of AM-1 cells by plasma membrane biotinylation and immunofluorescence analysis. (elsevierpure.com)
Immunohistochemical analysis of clinical samples of ameloblastoma also showed plasma membrane-localized V-ATPase and CLC-7 in the epithelium of plexiform, follicular and basal cell types. (elsevierpure.com)
Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. (accela.eu)
The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. (accela.eu)
Once cells become apoptotic plasma membrane PS asymmetry is lost. (accela.eu)

Description: A sandwich ELISA kit for detection of Neuroepithelial Cell Transforming Gene 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (noveoninc.com)
This ELISA kit is a solid phase ELISA designed for quantitative determination of Growth Factor Receptor Bound Protein 14 (Grb14). (anticorps-enligne.fr)

But it might open also interesting opportunities for in vivo applications. (jove.com)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
60][61][62] Furthermore, keratinocytes are the first cells to be exposed to potential irritants in vivo. (researchgate.net)
In addition to in vivo and isolated microvessel methods, the cultured endothelial cell monolayer protocol is an important tool that allows for understanding the specific, endothelial subcellular mechanisms that determine permeability of the endothelium to plasma proteins. (bvsalud.org)

The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)

The inhibition of vessel formation is an active area of cancer research given the central role angiogenesis plays in tumor growth. (biolegend.com)
Chemotaxis of metastatic tumor cells: Clues to mechanisms from the Dictyostelium paradigm. (godman-inc.com)

Background: Vascular endothelial growth factor C is a VEGF. (antibodies-online.com)
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. (antibodies-online.com)
This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation. (antibodies-online.com)
We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. (nature.com)
The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively. (biomatik.com)
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA). (biomatik.com)
No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed. (biomatik.com)
This protocol describes a simple and an economical method for isolation of endothelial cells from human umbilical vein. (bvsalud.org)
Thus, umbilical vein is an ideal source for isolation of endothelial cells of human origin. (bvsalud.org)
Endothelial cells are isolated from umbilical vein by collagenase digestion. (bvsalud.org)
This is followed by their culture on extracellular matrix (ECM) protein coated tissue culture flasks in presence of endothelial cell growth medium. (bvsalud.org)
In the last few decades, human umbilical vein endothelial cells (HUVECs) have come to be regarded as a standard model system by vascular biologists to understand general principles of endothelial cell biology and dysfunction. (bvsalud.org)
Advances in molecular biology over the past few decades have facilitated the generation of fusion proteins in which fluorescent reporters based upon the structure of green fluorescent protein can be linked to proteins found in human endothelial cells, such as VE-cadherin or ß-actin. (bvsalud.org)

Cold atmospheric plasma (CAP) treatment is a rapidly expanding and emerging technology for cancer treatment. (mdpi.com)
However, recent progress in atmospheric plasmas has led to the creation of cold plasmas with ion temperature close to room temperature. (nature.com)

The Global Peripheral Blood Mononuclear Cells Market is segmented based on product, application, technique, source, region, and competitive landscape.Based on Product, the market can be split into Cryopreserved or Frozen PBMC, Cultured or Fresh PBMC and peripehral blood mononuclear cell isolation & viability kits. (yahoo.com)

Expression levels of RANKL assessed by western blotting were markedly lower in ameloblastoma (AM-1) cells than in highly metastatic oral squamous cell carcinoma (HSC-3) cells. (elsevierpure.com)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)

These characteristics would facilitate the traditional meat characteristics simulation of cell cultured meat in term of texture and microstructure. (bvsalud.org)

Using Dyadic's proprietary filamentous fungal based microbial protein production platforms, the Company has been able to successfully develop stable cell lines to produce animal-free recombinant bovine serum albumin and recombinant human serum albumin at high levels. (itbusinessnet.com)
Today, serum albumin can be manufactured without blood plasma through recombinant protein production technology. (itbusinessnet.com)
Recombinant serum albumin provides high-quality consistency reducing time and offering high-grade performance for applications where protein contaminants are undesirable. (itbusinessnet.com)
SUMMARY Different nutritional disorders prevail at different stages in the life cycle, e.g. growth retardation in the fetus, protein energy deficiency in children, noncommunicable conditions in adults. (who.int)
These fusion proteins have been found to incorporate into structures alongside their native protein counterparts, allowing the dynamic visualization of how these subcellular structures are modified when cells are challenged with stimuli such as inflammatory mediators. (bvsalud.org)

On the cellular level, plasma effects include detachment of cells from the extracellular matrix and decreased migration velocity of cells. (nature.com)
Sulfated polysaccharides (SPs) are anionic carbohydrate polymers synthesized as extracellular or cell wall components by most of the algae and have potent bioactive properties. (brjnmims.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)

Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
MARS platform provides an easy and cost-effective protocol for CD34+ cell isolation. (accela.eu)

Human serum albumin (HSA) and bovine serum albumin (BSA) are proteins that are produced by the liver and extracted from blood plasma. (itbusinessnet.com)
While laminin and fibronectin are widely used ECM proteins, the one that your cell needs should be determined by test experiments. (sigmaaldrich.com)
In the present project (No 109-08 ) the utility of blood serum or plasma proteins for FBS replacement was investigated. (forschung3r.ch)
As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. (gentaur.com)
Vaccination is based on the principle of altering of immunity by inducing the multiplication of specialized cells, mainly lymphocytes, which either kill virus-infected cells by direct contact or through proteins they secrete. (globenewswire.com)
Membranes and Cell Movement: Interactions of Membranes with the Proteins of the Cytoskeleton. (godman-inc.com)
When microvascular hyperpermeability becomes uncontrolled or chronic, the excessive escape of plasma proteins into the surrounding tissue disrupts homeostasis and ultimately leads to organ dysfunction. (bvsalud.org)

Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
Conventionally, MSCs isolated from cord tissues are maintained and propagated in FBS containing medium for promotion of growth and survival of cells. (brjnmims.org)
The cells are immersed in a culture medium, which includes vital nutrients and energy reservoirs essential for the cells' survival. (microbiologynote.com)
1991 were contemporaneous with that of FAV 007 and were plasma and mycoplasma DNA and did not support its survival. (cdc.gov)

Nakamura M, Chikama T, Nishida T. Synergistic effect with Phe-Gly-Leu-Met-NH2 of the C-terminal of substance P and insulin-like growth factor-1 on epithelial wound healing of rabbit cornea. (medscape.com)

To enhance efficiency and expand the applicability of the CAP method for brain tumors and reduce the gas flow rate and size of the plasma jet, a novel micro-sized CAP device (µCAP) was developed and employed to target glioblastoma tumors in the murine brain. (mdpi.com)
In this research, Neuro2a cells that are murine neuroblastoma line derived from AJ mice is cultured in DMEM media. (tamucc.edu)

An osmolarity testing device is used to test a variety of solutions such as blood, plasma, urine, serum, cell culture media and others. (medgadget.com)
[ 1 ] Urine osmolality is greater than plasma osmolality. (medscape.com)
Other cultures use urine from cows or confidence, P was the expected prevalence, and d was other animals, and some even use human urine ( 16 , 20 ). (who.int)

Thus, the secondary metabolite mediated effect of Pseudomonas isolates on Verticillium species results in a complex transcriptional response, leading to decreased growth with precautions for self-protection combined with the initiation of a change in fungal growth direction. (frontiersin.org)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)

This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. (nature.com)

Stem cell researchers value its role in maintaining pluripotency. (sigmaaldrich.com)
In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

This medium was developed by Murashige and Skoog to induce organogenesis, and regeneration of plants in cultured tissues. (microbiologynote.com)

For the quantitative determination of rabbit Grb14 concentrations in serum, plasma,cell culture supernates and tissue homogenate. (anticorps-enligne.fr)

Breast metastatic tumors in lung can be substituted by lung-derived malignant cells transformed by alternative splicing H19 lncRNA. (medscape.com)

Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)
Recent studies have delineated the effects of cold plasma on both the cellular and sub-cellular levels. (nature.com)
In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)
To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)
Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes. (accela.eu)
Silicon nanowires can be used as one-dimensional semiconductors for sensing charged molecules in a biological sample and nanopillars can enhance cellular growth and measure minute mechanical stresses. (lu.se)
I will show how these nanostructures can be utilized for extremely sensitive virus detection, enhanced cellular growth, extracting polluting chemicals from the groundwater and mechanical stress sensors. (lu.se)

Experiments coculturing mouse macrophages (RAW264.7) with AM-1 demonstrated low osteoclastogenic activity, as assessed by tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cell formation, probably because of low release of RANKL, whereas cocultures of RAW264.7 with HSC-3 cells exhibited very high osteoclastogenic activity. (elsevierpure.com)

Description: serum, platelet-poor plasma, tissue homogenates, cell culture supernatants and other biological fluids. (tissue-cell-culture.com)

Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

Along with Dr. Alexis Carrel, a surgeon at Rockefeller Institute (1906 - 1927), Dr. Burrows is credited with coining the phrase "tissue culture", and is among the first to adapt such methods to the study of tissues from warm-blooded animals. (wikipedia.org)
Among the propositions was the idea that their chick heart cultures could live indefinitely by continuous application of their methods. (wikipedia.org)
Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
This method provides a new tool in the technologies field seeing as it represents a valuable alternative to existing methods such as optogenetics and thermal stimulation for optical control of living cells activity. (jove.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)

Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)

It is an important building block of all cell membranes, and an important metabolite in many biosynthetic pathways. (forschung3r.ch)
The study on PBMC cells is mandatory to understand the biology and pathology related pathways as well as in clinical research, research on life threatening diseases, immunology, vaccine development, etc.PBMCs provide information on effects of prospective novel medication molecules on people, particularly on their immune systems. (yahoo.com)
A number of studies have explored how regulation of generally, and at least to a first approximation, it is obvious that metabolic pathways affects the growth rate of microorganisms, gene regulation only is useful if the environmental conditions vary both in the steady state and in response to changes in the local with time. (lu.se)

MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Recently, MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment. (techscience.com)
The current study is designed to compare the effect of cord blood plasma (CBP) with MSC qualified FBS (M FBS) during culture and cryopreservation of MSCs. (brjnmims.org)
However, cord MSCs displayed slow growth rate and reduction in surface expression of CD105 marker in CBP, whereas, the other parameters were comparable. (brjnmims.org)

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. (antibodies-online.com)

Plant tissue culture is a technique that is utilized to sustain or grow plant cells, tissues, or organs in a sterile condition on a nutrient culture medium of known composition. (microbiologynote.com)

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. (accela.eu)
The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. (accela.eu)
Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. (accela.eu)

In this study, we examined the therapeutic potential of a cold plasma jet in cancer cell lines and tumours, focusing on selective tumour cell eradication capabilities and signalling pathway deregulation. (nature.com)

Application of umbilical cord serum eyedrops for the treatment of neurotrophic keratitis. (medscape.com)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

In this chapter, two variations of the popular Transwell culture methodology to determine permeability to using fluorescently labeled tracers are presented. (bvsalud.org)

It can be found in human serum, follicular fluid, and Among the essential components that affects the plasma [11-13]. (who.int)

The effects of different fluorescent pseudomonads, including some known biocontrol agents of other plant pathogens, on fungal growth of the haploid Verticillium dahliae and/or the amphidiploid Verticillium longisporum were compared on pectin-rich medium, in microfluidic interaction channels, allowing visualization of single hyphae, or on Arabidopsis thaliana roots. (frontiersin.org)

There is still some controversy with respect to the mechanism of plasma-cell interaction. (nature.com)
Even less clear is the nature of the interaction between cold plasmas and cancer tissue. (nature.com)
For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. (homeodomains.com)
Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. (accela.eu)

For instance, bead-based Luminex xMAP multiplex assay provides a platform for cancer biomarker assays, which are compatible with plasma, serum, cultured cells, and other biological samples. (articlepedia.xyz)

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)

1) One is to replace serum by other biological but as yet inadequately defined mixtures such as extracts from plants, animal tissues or cells. (forschung3r.ch)
The technique in which the tissues or cells are grown on an artificial medium separate from the parent organism is known as tissue culture. (microbiologynote.com)

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neuroepithelial Cell Transforming Gene 1 (NET1) in Tissue homogenates and other biological fluids. (noveoninc.com)
NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. (gentaur.com)
Population is gaining awareness regarding the use of peripheral blood mononuclear cells in cell and gene therapy.Hence, the extraction and usage of peripheral blood mononuclear cells prove to be the key driving force for the market growth. (yahoo.com)
A very natural place to study gene detecting and metabolizing lactose, it is known that the overall regulation is in the metabolism of the cell, and then specifically in effect of expressing the lac genes in vain is a drop in the growth rate the regulation of genes that code for enzymes and transporter of as much as 5% [1,6]. (lu.se)

However, AM-1 cultured directly on calcium phosphate-coated plates formed resorption pits, and this was inhibited by application of bafilomycin A1. (elsevierpure.com)

Riboflavin supports cell growth and metabolism, aiding researchers in R&D. In cell culture, it is often found as a key ingredient in media formulations. (sigmaaldrich.com)

Exogenous proline and glycinebetaine increase NaCl-induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycine betaine in tobacco Bright Yellow-2 suspension cultured cells. (arccjournals.com)
Gamborg developed this medium for cell suspension and callus cultures. (microbiologynote.com)

Description: A sandwich quantitative ELISA assay kit for detection of Chicken Transforming Growth Factor Beta 1 (TGFb1) in samples from serum, plasma or other biological fluids. (tissue-cell-culture.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. (stemcellcharter.org)
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. (stemcellcharter.org)

Patients now have a better alternative to cell-based therapies with respect to traditional regenerative medicines, but their side effects can hinder their usage.Developed regions such as North America and Europe have a strong presence of cell therapies for human well-being. (yahoo.com)
One novel approach to restore tooth structure is based on regenerative endodontic procedures by application of tissue engineering. (bvsalud.org)
Regenerative endodontic procedures can be defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp-dentin complex. (bvsalud.org)
Regenerative dental procedures have a long history, originating around 1952, when Hermann reported on the application of Ca(OH)2 in a case report of vital pulp amputation 13 . (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)

Mr. Hazelton continued, "With the global albumin market valued at US$ 5.7 billion in 2022 and expected to reach US$ 8.2 billion by 2028, we believe this market represents a significant growth opportunity for Dyadic. (itbusinessnet.com)
The requirement of high dosage of serum albumin in clinical applications is expected to continue to drive demand for economical large-scale production of animal-free recombinant serum albumin which provides us the opportunity to leverage our microbial expression platforms to potentially develop and commercialize multiple end-market serum albumin products in both pharmaceutical and non-pharmaceutical applications. (itbusinessnet.com)
Recombinant serum albumin is purified, bacteria-free, animal component-free, and virus-free and is structurally equivalent to plasma-derived serum albumin. (itbusinessnet.com)
In addition, recombinant serum albumin is more homogeneous than plasma-derived serum albumin and lacks immunogenicity and toxicity while offering a distinct and uniform medium. (itbusinessnet.com)
Factors driving market growth include increase in the adoption of albumin products, growth in awareness about recombinant albumin products, and increasing use of albumin in other applications such as an excipient and drug formulating agent. (itbusinessnet.com)
Increasingly, recombinant serum albumin is being used as an alternative to plasma-derived albumin in human therapeutics, cell culture media, diagnostic test kits, and research & development. (itbusinessnet.com)
During riboflavin localization in plasma, it is bound to albumin and specific immunoglobulins. (sigmaaldrich.com)

Anitua E, Zalduendo MM, Alkhraisat MH, Orive G. Release kinetics of platelet-derived and plasma-derived growth factors from autologous plasma rich in growth factors. (springer.com)
In April, it announced it grew cultivated chicken and duck meat without an animal-based media-and at a fraction of the cost compared to using animal-based growth factors. (greenqueen.com.hk)
The growth in this market is majorly driven by factors such as strong pipeline for vaccines, increasing government focus on immunization programs, and technological progress in vaccine administration. (globenewswire.com)
However, high capital investment and dearth of skilled labors are the major factors that hinder the market growth. (articlepedia.xyz)
ü A comprehensive analysis of the factors that drive and restrict the market growth is provided. (articlepedia.xyz)
Embryonic signals also alter the expression of a large number of transcription factors, growth factors and their receptors and integrins. (brjnmims.org)
The TX-HYB Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. (homeodomains.com)
Published September 5, 2007 transcription factors and replicating extra DNA, rather than going directly into growth of the cell. (lu.se)

However, optimizing the concentrations that work for your cells is recommended. (sigmaaldrich.com)
In the current study, Chlamydomonas reinhardtii (Cr) cells were attributed to sodium nitrate stress in concentrations such as 5 mM, 10 mM, 20 mM, 30 mM, and a control to determine the productivity and bioactivity of SPs. (brjnmims.org)

Triplicate samples of 104 neuroblastoma cells were plated and treated with cold plasma technology. (tamucc.edu)

There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

At Yale, Burrows also successfully established tissue cultures of embryonic chick cells. (wikipedia.org)

Due to its clinical applications success of IVF results is the morphological and the expansion of non-invasive treatment quality of the embryo. (who.int)

These improvements include the substitution of blood plasma for lymph and the addition of embryo extract in the tissue culture medium that improved the growth of tissue cultures. (wikipedia.org)
Because the tissue cultures far outlived their organisms, they were deemed "immortal. (wikipedia.org)
the medium was developed by Chu for the cereal anther culture, besides other tissue cultures. (microbiologynote.com)

Anatomy14

Organisms11

Diseases5

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Psychiatry and Psychology1

Phenomena and Processes40

Disciplines and Occupations5

Technology, Industry, Agriculture7

Information Science10

Health Care15

Vitro14

Proliferation13

Supernatant3

Differentiation11

Scaffolds6

Platelet-rich plasma4

Morphology5

Monolayers2

Mesenchymal Stem3

Membrane10

ELISA Kit2

Vivo4

Fetal bovin2

Tumor2

Endothelial15

Atmospheric2

Viability1

Squamous cell carc2

Characteristics1

Protein5

Extracellular3

Isolation3

Proteins9

Survival5

Epithelial1

Murine2

Urine3

Transcriptional2

Polymers1

Stem cell6

Induce1

Supernates1

Metastatic1

Cellular8

Experiments1

Supernatants1

Populations1

Methods5

Embryonic cells1

Pathways3

MSCs4

Sandwich ELISA1

Organs1

Apoptosis3

Therapeutic1

Umbilical2

Permeability1

Human serum1

Fungal growth1

Interaction4

Biological samples1

Serum-free media1

Tissues or cells2

Gene4

Calcium1

Metabolism1

Suspension2

Detection3

Regenerative6

Albumin7

Factors8

Concentrations2

Samples1

Regeneration1

Chick1

Clinical applications1

Tissue cultures3