Plant genetic transformation genetic. Medical search. Frequent questions

Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. (eurekamag.com)
An efficient transformation system of Veratrum dahuricum mediated with Agrobacterium tumefaciens is presented. (vtt.fi)
Direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation of Solanum trilobatum L. (tubitak.gov.tr)
An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. Trilobatum was established for the first time. (tubitak.gov.tr)
The Agrobacterium strain EHA 105 harboring the binary vector pCAMBIA 1301, which contained hygromycin phosphotransferase (hpt) as a selectable marker gene and β-glucuronidase (gus) as a reporter gene, was used for the transformation. (tubitak.gov.tr)
Researchers have been using biolistics in delivering the genome editing complex in wheat due to difficulties in Agrobacterium -mediated genetic transformation in the species. (isaaa.org)
However, scientist Shujuan Zhang from Shandong Academy of Agricultural Sciences in China and colleagues overcome this limitation by successfully targeting three wheat genes using CRISPR-Cas9 delivered via Agrobacterium -mediated genetic transformation. (isaaa.org)
Agrobacterium -mediated genetic transformation is considered to be better than biolistic delivery because the latter produces single and more specific insertions of the genome editing complex in the genome and does not require expensive equipment. (isaaa.org)
These results show the applicability of Agrobacterium -mediated genetic transformation in wheat genome editing and genetic improvement. (isaaa.org)
Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)
In pursuit of this vision, Veena went on to study at Purdue University and the Danforth Center, focusing on the molecular basis of Agrobacterium -mediated transformation to improve the efficiency of genetic transformation of economically important plants and to prevent crown gall disease on grape and other susceptible plant species. (danforthcenter.org)
Additionally, Dr. Zhang developed highly efficient approaches for cotton somatic embryogenesis, plant regeneration and highly efficient Agrobacterium-mediated genetic transformation systems in cotton. (icac.org)
i) long distance RNA trafficking between parasitic plants and their hosts, ii) RNA interference (RNAi) as a resistance mechanism against parasites - Striga and Cuscuta, iii) genetic engineering strategies for enhancing drought tolerance in tropical crops, iv) Agrobacterium rhizogenes transformation of cereals as a functional genomics tool for plant-parasite interactions, and v) molecular mechanisms of parasitic plants/host plants interactions. (ac.ke)
GoldBio's EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. (goldbio.com)

On the other hand, transgenic plants produced from mature tissue via genetic transformation can drastically shorten flowering and fruiting time and thus speed up field evaluation studies. (springer.com)
Phosphinothricin (PPT, 16 mg/L) was used as the selectable agent, and a transformation efficiency of 15% (transgenic plants/100 infected calli) was obtained. (vtt.fi)
The steroid alkaloids cyclopamine, jervine, and veratramine were detected in transgenic plants, in non-transformed and control plants collected from natural sites. (vtt.fi)
Current trends and future prospects for optimization of excision-activation machinery and its practical implementation for the generation of transgenic plants and plant products free of undesired genes are discussed. (bioone.org)
The transgenic plants were confirmed by histochemical gus analysis, PCR, and RT-PCR analysis. (tubitak.gov.tr)
Alternatively, qPCR requires less DNA and is potentially simpler to perform, but its results can lack the accuracy and precision needed to confidently distinguish between one and two copy events in transgenic plants with large genomes. (usda.gov)
HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
1989). Genetic transformation may enable the development of transgenic plants with enhanced resistance to wilt diseases. (scialert.net)
The book dwells on aspects of genome editing which will enable researchers to produce transgenic plants in a more convenient and safer way to genetic modification of stem cells holding significant therapeutic promise to treat complications of diabetes and obesity. (intechopen.com)
Her core facility collaborates with all of our Principal Investigators, helping them test and develop transgenic plants to further their research. (danforthcenter.org)
Matheka JM, Anami S, Gethi J, Omer RA, Alakonya A, Machuka J, Runo SM A new double right border binary vector for producing marker-free transgenic plants. (ac.ke)

Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
Current areas of focus of nanotechnology development in the agricultural industry include development of environmentally conscious nano fertilizers to provide efficient ion, and nutrient delivery into plant cells, and plant gene transformations to produce plants with desirable genes such as drought resistance and accelerated growth cycles. (wikipedia.org)
17. Yanofsky M F. Floral meristems to floral organs:genes controlling early events in Arabidopsis flower development[J].Annu Rev Plant Physiol Plant Mol Biol,1995,46:167-188. (nefu.edu.cn)
19. Mara C D,Huang T,Irish V F. The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA negatively regulate the BANQUO genes implicated in light signaling[J].Plant Cell,2010,22(3):690-702. (nefu.edu.cn)
The expression of genes encoded within the T-DNA promotes the development and production of roots at the site of infection on most dicotyledonous plants. (bioone.org)
Various approaches to avoid or eliminate undesirable transgenes, most notably selectable marker genes used in plant transformation, have recently been developed. (bioone.org)
These approaches include cotransformation with two independent T-DNAs or plasmid DNAs followed by their subsequent segregation, transposon-mediated DNA elimination, and most recently, attempts to replace bacterial T-DNA borders and selectable marker genes with functional equivalents of plant origin. (bioone.org)
This review focuses on recent reports demonstrating the elimination of undesirable transgenes (essentially selectable marker and recombinase genes) from the plant genome and concomitant activation of a silent transgene (e.g., a reporter gene) mediated by different site-specific recombinases driven by constitutive or chemically, environmentally or developmentally regulated promoters. (bioone.org)
Such "single-copy transformants" are desirable because they are usually more stable in both the inheritance and expression of the added genes than transformed plants with multiple copies. (usda.gov)
By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)
Based on policy discussions, it is possible that the first-generation plants containing the edited genes will not require extensive regulation, and these tools will be extremely valuable in the long-term efforts of every strawberry breeding program. (ufl.edu)
The VPZ construct contains three genes that code for proteins of the xanthophyll cycle, which is a pigment cycle that helps in the photoprotection of the plants. (agrimarketing.com)
While at Monsanto (now Bayer) as a Senior Scientist, she contributed towards the development of novel, high-throughput tools for the evaluation of genes, promoter elements for binary vectors, genome-modification technologies for the plant transformation pipeline, and the establishment of the selectable-marker free transgenic pipeline. (danforthcenter.org)
The coding regions are obviously important - they're what makes the actual meaty, bony, brainy part of the animal, or the leaves and roots and flowers of a plant - but the thing is, we primates share almost all of the same genes with a mouse, which makes one wonder what makes us different from a mouse. (freethoughtblogs.com)
Hence there is a need to diversify the genetic basis of the locally adapted germplasm by introgressing genes responsible for improving tolerance to drought stress. (scialert.net)
In essence, "genetic modification" or "genetic engineering" techniques enable scientists to find individual genes that control particular characteristics, separate them from the original source, and transfer them directly into the cells of an animal, plant, bacterium, or virus. (hindawi.com)
Substantial genetic and biochemical similarities exist between resistance determinants in antimicrobial agent-producing actinomycetes and resistance genes found in gram-positive and gram-negative pathogens ( 6 - 9 ). (cdc.gov)
Some researchers have proposed that the vanH , vanA , and vanX genes of hospital enterococci may have been acquired en bloc from the actinomycetes ( 8 ). (cdc.gov)
Genetic polymorphism of CYP genes, alone or in combination, as a risk modifier of tobacco-related cancers. (cdc.gov)

The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
Our results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. (springer.com)
With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
Knowledge of the promoters used in gene constructs for genetic transformation is essential for successfully applying GM technology. (medwelljournals.com)
The transgenic nature of the regenerated plants was confirmed by PCR and Southern blot analysis, and expression of the bar gene was detected by RT-PCR and Quick PAT/bar strips. (vtt.fi)
Because many organic functions such as ion exchange and plant gene expression operate on small scales, nanomaterials offer a toolset that works at just the right scale to provide efficient, targeted delivery to living cells. (wikipedia.org)
The flower is the main reproductive organs of the plants, plays an extremely important role in the process of breeding and the PISTILLATA(PI) gene, and as a member of the B functional gene, plays an important role in floral organ development. (nefu.edu.cn)
In order to explore the function of PI gene in the development of petals and stamens, the PI gene in Arabidopsis thaliana was used as the research object, and the AtPI gene was amplified from cDNA by PCR, and the plant expression vector(pROKⅡ- AtPI ) was constructed and transformed into tobacco. (nefu.edu.cn)
Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana AtPI Gene in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2016, 36(3): 388-394. (nefu.edu.cn)
As a result, hairy roots are widely used as a trans-genic tool for the production of metabolites and for the study of gene function in plants. (bioone.org)
While transformation procedures have become routine for a growing number of plant species, the random introduction of complex transgenenic DNA into the plant genome by current methods generates unpredictable effects on both transgene and homologous native gene expression. (bioone.org)
This method provides an experimentally validated resource that the plant research community can use for simple and accurate determination of gene copy numbers in plant genomes. (usda.gov)
The risk of cancer is highlighted by the recent report that gene therapy - genetic modification of human cells - claimed its first cancer victim [4]. (i-sis.org.uk)
With gene editing, the final product can match that obtained by conventional plant breeding, but in a much shorter timeframe. (ufl.edu)
For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
She was working on functional characterization of gene called glyoxalase I. In mammals, this gene was understood to be involved in Alzheimer's and cancer, but no one knew what this gene did in plants. (danforthcenter.org)
When the gene is overexpressed in a plant, it can tolerate adverse soil conditions. (danforthcenter.org)
Kirigia D, Runo S, Alakonya A. A virus-induced gene silencing (VIGS) system for functional genomics in the parasitic plant Striga hermonthica. (ac.ke)
McHughen has succeeded in providing the reader with a comprehensive discussion on what is genetically modified food.Written for the lay audience, he has included well written and easy to understand descriptions of molecular genetics, gene banks, the polymerase chain reaction, genetic transformation, a history of genetic engineering, and the fundamentals of plant breeding (the … Be the first to write a review. (newideas.net)
The change in the cell's genetic material may occur spontaneously as a random event or due to a gene mutation or be brought on by an external exposure to a substance that causes cancer (a carcinogen). (msdmanuals.com)

One major limitation associated with existing protocols when used on economically important citrus species is likely low plant regeneration frequencies. (springer.com)
This review presents a summary of the different types of promoters with examples of some of them that have been identified and characterized for genetic transformation of various plant species. (medwelljournals.com)
The risk of transgene transfer into related plant species and consumers is another concern associated with the conventional transformation technologies. (bioone.org)
This method and the compendium of probes and primers will be a useful resource for the plant research community, enabling the simple and accurate determination of transgene copy number in these six important crop species. (usda.gov)
It is designed to cross species barriers and to jump into genomes, and it has homologies to the DNA of many species and their genetic parasites (plasmids, transposons and viruses), thereby enhancing recombination with all of them [2]. (i-sis.org.uk)
Unfortunately, although the CaMV virus is specific for plants, its 35S promoter is active in species across the living world, human cells included, as we discovered in the scientific literature dating back to 1989. (i-sis.org.uk)
Transgenic technology' refers to the transfer of a genetic material from one species to another. (ufl.edu)
1994). Availability of a tissue culture protocol with such criteria would be an important step towards the establishment of a genetic transformation system in this species. (scialert.net)
The book throws light on new transformation strategies which can be used to increase the transformation efficiency in most plant species. (intechopen.com)
The plant is one of the most important model plant species in the world, sought out by an ever-increasing number of research laboratories for a range of studies, due in part to its ease of growth and ease to genetic transformation. (lincoln.ac.nz)
Modern biotechnology has allowed the movement of genetic material across unrelated species, something impossible with the traditional breeding methods. (hindawi.com)
The application of genetic modification allows genetic material to be transferred from any species into plants or other organisms. (hindawi.com)
We want to understand how observed genetic variation relates to species limits, how variation is maintained and distributed throughout populations or selected for within single infections. (lu.se)
Landscape transformation, invasive organisms, climate change and other human-induced changes lead to altered population sizes and species composition - changes in biodiversity - which in turn can negatively affect functions such as carbon capture, pollination and pest control. (lu.se)
Over just a few generations, the genetic composition of species can change in response to environmental changes, altering not only the overall species composition but also the adaptation of individual species. (lu.se)

On the other hand, nanofertilizers are able to amend this issue because of their high absorption efficiency into the targeted plant- which is owed to their remarkably high surface area to volume ratios. (wikipedia.org)
A stable transformation efficiency of 64% was achieved under optimized transformation conditions. (tubitak.gov.tr)
After more than a decade of working toward this goal, a collaborative team led by the University of Illinois has transgenically altered soybean plants to increase the efficiency of photosynthesis, resulting in greater yields without loss of quality. (agrimarketing.com)
0.01) in callus induction frequency, formation of embryogenic callus, number of shoots per embryogenic callus regenerating plants, percent of embryogenic callus forming shoots and regeneration efficiency. (scialert.net)
Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol given below. (goldbio.com)
Transformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 µg of plasmid into a given volume of competent cells. (goldbio.com)

A key characteristic of hairy roots is their ability to grow quickly in the absence of exogenous plant growth regulators. (bioone.org)
To identify the optimal conditions for shoot and root regeneration for 'Florida Radiance', Sweet Sensation® 'Florida127' and 'Florida Brilliance', explants were grown on a range of media with varying compositions of plant growth regulators. (ufl.edu)
Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. (scielo.br)
The effect of different media and various combinations of Plant Growth Regulators (PGRs) on callus formation and shoot regeneration were investigated. (scialert.net)

Propelled by rapid advances in genomic technologies, recent studies with duckweed again highlight the potential of these small plants to enable discoveries in diverse fields from ecology to chronobiology. (osti.gov)
Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning[J].Proc Natl Acad Sci USA,1999,96(11):6535-6540. (nefu.edu.cn)
This suggests some of the extra energy gained from improved photosynthesis was likely diverted to the nitrogen-fixing bacteria in the plant's nodules," said RIPE Director Stephen Long, Ikenberry Endowed University Chair of Crop Sciences and Plant Biology at Illinois' Carl R. Woese Institute for Genomic Biology. (agrimarketing.com)
However, compared to other major cereals, modern genetic and genomic research is lagging for millets although they possess excellent agronomical and nutrient-supplementation traits. (rajagiri.edu)
In our group, we are interested in evolutionary, functional and behavioural ecology questions using molecular, genetic and genomic methods. (lu.se)

1990 Visiting Scientist in plant molecular biology, University of California, Riverside. (cornell.edu)
Lincoln University Associate Professor in Plant Genomics and Molecular Biology, Dr Chris Winefield , is an associate investigator in the Australian Research Council Centre of Excellence for Plant Success in Nature & Agriculture. (lincoln.ac.nz)
Invited Review-Physiology and Molecular Biology of Plants. (danforthcenter.org)
I teach mainly plant and molecular biology at different levels. (lu.se)

In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)
The first step is to develop and optimize a tissue culture and transformation system (protocols to introduce foreign DNA to new plants) for UF strawberry lines, so that new plants can be regenerated from cells containing introduced DNA. (ufl.edu)
Waweru D, KuriaE, BradleyJ, Scholes J, Runo S. Tissue Culture Protocols for the Obligate Parasitic Plant Striga hermonthica and Implications for Host-Parasite Co-cultivation. (ac.ke)
During those years I used and developed different applications, such as cell and tissue culture,genetic transformations and cell fusions in order to improve different agricultural characters in different crops. (lu.se)

I have been drawing our regulators' attention to it at least since 1996 [1], when there was already sufficient evidence to suggest that transgenic DNA in GM crops and products can spread by being taken up directly by viruses and bacteria as well as plant and animals cells. (i-sis.org.uk)
In this context, the objective was to evaluate the effect of pre-germination treatments with plant regulators and bioactivator in melon seeds for the production of seedlings irrigated with biosaline water from fish farming effluent. (bvsalud.org)

Their tiny size, rapid growth by clonal propagation, and facile uptake of labeled compounds from the media were attractive features that made them a well-known model for plant biology from 1950 to 1990. (osti.gov)
11. Theissen G,Saedler H. Plant biology. (nefu.edu.cn)
This method can help answer key questions in the plant molecular and cell biology field such as, how can we proteins in the cell. (jove.com)
For more information, read the article in BMC Plant Biology . (isaaa.org)
Invited review-In Vitro Cellular and Developmental Biology-Plant 43: 383-403. (danforthcenter.org)

Interest in duckweed has steadily regained momentum over the past decade, driven in part by the growing need to identify alternative plants from traditional agricultural crops that can help tackle urgent societal challenges, such as climate change and rapid population expansion. (osti.gov)
Plant geneticists who have incorporated the promoter into practically all GM crops now grown commercially are apparently unaware of this crucial information [5]. (i-sis.org.uk)
Do GM crops with more complex transformation contribute to the enrichment of multinationals? (intechopen.com)
Genetic engineering through plant transformation is foundational to improving crops, from increasing the nutritional value of cassava, to improving the drought tolerance of corn. (danforthcenter.org)
3 Those reviews, which focused almost entirely on the genetic aspects of biotechnology, concluded that GM crops pose no unique hazards to human health. (cornucopia.org)
These "Roundup-Ready" crops now account for more than 90% of the corn and soybeans planted in the United States. (cornucopia.org)
For example, the simplification of the agricultural landscape in the last century may have resulted in the disadvantage of specialized pollinators, with negative effects on the pollination of wild plants or crops that need these pollinators. (lu.se)

The factors that influence the transformation frequency were optimized as follows: age of explants (40 days old), acetosyringone concentration (150 μM), duration of preculture (2 days), bacterial optical density (O.D. 0.4), Tween 20 concentration (0.01%), infection time (15 min), pH of cocultivation medium (5.4), and cocultivation duration (3 days). (tubitak.gov.tr)
Plant regeneration from explants in a short period of time bypassing a long intermediate callus phase could reduce somaclonal variation (Skirvin et al . (scialert.net)
Her patent on soybean transformation and regeneration using half plant explants (US Patent #7,473,822, January 6, 2009) is highly cited by those working in plant transformation. (sivb.org)

An aggressive promoter from a virus is often used to boost the expression of the transgene, in animal and human cells, from the cytomegalovirus that infects mammalian cells, and in plants, the 35S promoter from the cauliflower mosaic virus (CaMV) that infects Cruciferae plants. (i-sis.org.uk)

The researchers first tested their idea in tobacco plants because of the ease of transforming the crop's genetics and the amount of seeds that can be produced from a single plant. (agrimarketing.com)
M.S. 1978 Plant Breeding & Genetics, Univ. (cornell.edu)
Ph.D . 1980 Plant Breeding & Genetics, Univ. (cornell.edu)
He graduated from China Agricultural University with a degree in plant genetics and breeding. (icac.org)
I have published on such topics as microbial genetics, genetic engineering, and the future of man and science. (roundtablegroup.com)
I have 26 years of experience and specialize in the field of genetics, with specific knowledge of agriculture, human identification and plant identification. (roundtablegroup.com)
Attempts are made in classical breeding via forward genetics to detect the genetic architecture of a trait from its expression (phenotype). (researchgate.net)

Thus, the protocol can be used for genetic improvement of this plant in the future. (tubitak.gov.tr)
Technical abstract: Genetic transformation is a powerful means for the improvement of crop plants, but requires labor and resource intensive methods. (usda.gov)
Program objectives focus on the genetic improvement of grapevines while integrating traditional and novel techniques. (cornell.edu)
Novel techniques in plant breeding are being studied for potential contributions to grapevine improvement. (cornell.edu)
As frontiers of crop production, plant physiologists are the most responsible for the improvement of crop production steadily. (techscience.com)
That study blew me away because it helped me understand the value and potential of genetic engineering in crop improvement. (danforthcenter.org)
Dr. Wang's research program at Iowa State University continues to focus on developing novel genetic transformation technologies for crop improvement. (sivb.org)
Conventional and Molecular Breeding for Genetic Improvement of Maize (Zea mays L. (researchgate.net)

Another beneficial aspect of using nanofertilizers is the ability to provide slow release of nutrients into the plant over a 40-50 day time period, rather than the 4-10 day period of conventional fertilizers. (wikipedia.org)
We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. (jove.com)
1994). Potential genetic variability for conventional breeding is limited in Cuminum cyminum (Hunault et al . (scialert.net)

Veena Veena has always paid attention to the natural diversity in plants, from color and shape, to size and development patterns. (danforthcenter.org)
Adding injury to infection: The relationship between injury status and genetic diversity of Theileria infecting plains zebra, Equus quagga. (ac.ke)
Genetic structure and diversity of East African taro [Colocasia esculenta (L.) Schott]. (ac.ke)
I have published on such topics as teaching evolution, genetic diversity and genetic markers. (roundtablegroup.com)
The Global Assessment of Biodiversity and Ecosystem Services by the UN's Intergovernmental Panel on Biological Diversity (IPBES) summarized research on how human transformation of the Earth's ecosystems is affecting all the ecosystem functions on which we depend. (lu.se)

Plant defensins also have been tested as biotechnological tools to improve crop production through fungi resistance generation in organisms genetically modified (OGM). (frontiersin.org)
He has been serving as co-editor-in-chief, associate editor and guest editor for 10 international journals, including Scientific Reports, Plant Biotechnology Journal and The Crop Journal. (icac.org)
The fundamental aspects of crop breeding are the identification, investigation, creation and utilization of genetic variation. (researchgate.net)

In this first-of-its-kind work, recently published in Science, the group improved the VPZ construct within the soybean plant to improve photosynthesis and then conducted field trials to see if yield would be improved as a result. (agrimarketing.com)
She worked for 7 years at ICI seeds, which became Syngenta, as a project leader working on the genetic transformation of corn and soybean. (sivb.org)
The Plant Transformation Facility at Iowa State rose to national prominence due to their innovative research program for transforming corn and soybean. (sivb.org)

Transgenic tobacco plants exist obvious phenotype compared to wild-type tobacco, such as smaller corolla, shorter stamens, and abnormal ovary, which is 5-10 mm longer than wild-type tobacco. (nefu.edu.cn)
Tobacco, being a non-food and non-feed plant, has been considered as a good choice for producing enzymes with applications in technical industry, using a transplastomic approach. (springer.com)
He worked with a team led by the Queensland University of Technology (QUT) to determine the genome sequences of two strains of native Australian tobacco Nicotiana benthamiana , a plant used worldwide for fundamental research and biotechnology. (lincoln.ac.nz)
EHA105 is ideal for genetic transformation of rice, tobacco and several other plants. (goldbio.com)

Strong Genetic Differentiation Between North American and European Populations of Phytophthora alni subsp. (unl.edu)
In our group, we ask questions on how natural and sexual selection shapes phenotypes in wild populations, why some populations are more evolvable than others and how the genetic architecture of traits constrain or facilitate evolution. (lu.se)
[ 4 ] The variable prevalence among different populations suggests genetic influences, although familial cases of nevus of Ota are exceedingly rare. (medscape.com)

Drought is one of the main causes of reduced growth and yield in these plants. (techscience.com)
Five Ethiopian tropical maize genotypes were evaluated for their regeneration potential through somatic embryogenesis to establish regeneration system and select regenerable genotypes as prerequisites in applying genetic transformation for improved drought stress tolerance. (scialert.net)

An efficient genetic transformation protocol is a key step for using transgenic technologies to improve citrus quality, productivity, and resistance to abiotic and biotic stresses such as HLB. (springer.com)

The use of site-specific recombination to remove undesired DNA from the plant genome and concomitantly, via excision-mediated DNA rearrangement, switch-activate by choice transgenes of agronomical, food or feed quality traits provides a versatile "transgene maintenance and control" strategy that can significantly contribute to the transfer of transgenic laboratory developments into farming practice. (bioone.org)

Solanum trilobatum L. İs one of the most valued medicinal plants of Indian Ayurveda and Siddha medicine. (tubitak.gov.tr)
Pumpkin is one of the well-known edible plants and has substantial medicinal properties due to the presence of unique natural edible substances. (researchgate.net)
The purpose of the present article is to discuss various medicinal and biological potentials of pumpkin that can impart further research developments with this plant for human health benefits. (researchgate.net)

From there, many plant defensins have been reported and studies on this class of peptides encompass its activity toward microorganisms and molecular features of the mechanism of action against bacteria and fungi. (frontiersin.org)
Herein, is a review of the history of plant defensins since their discovery at the beginning of 90s, following the advances on its structure conformation and mechanism of action towards microorganisms is reported. (frontiersin.org)
Our mission is to provide solutions to plant disease and health issues, develop new knowledge of plant-pathogenic and plant-associated microorganisms, and provide quality, relevant education. (unl.edu)
Environmental applications of microorganisms are wide and varied, ranging from bioremediation, biopesticides, nitrogen fixation, plant growth promoter, to biocontrol of plant diseases, and other such agricultural practices. (hindawi.com)
When I eventually returned to Lund University, I started to get interested in the interplay between microorganisms and plants on a molecular level. (lu.se)

The use of genetic engineering and modern techniques such as transcriptomics, proteomics and sequence analysis are included in the course. (lu.se)

Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches[J].Plant Mol Biol,2007,65(3):233-242. (nefu.edu.cn)
While the finished varieties do not contain DNA from other organisms, the process introduces genetic information that orchestrates the desired genetic change. (ufl.edu)
Understanding the genetic basis of a plant's qualitative and quantitative traits is essential for developing consumer-favored varieties. (bvsalud.org)

Abstract - - Crown gall was observed in 99% of the plant nurseries surveyed in the major fruit tree production region of Algeria. (agronomy-journal.org)
La galle du collet ou crown gall sévit dans 99% des pépinières de production de plants fruitiers de l'Algérois. (agronomy-journal.org)
Les espèces les plus touchées sont le pêcher (5,39%), l'amandier (3,43%), le cerisier (1,50%), le pommier (1,47%) et l'olivier (1,30%) (Tableau I). Au sein d'une même espèce, la proportion de plants atteints de crown gall varie en fonction du porte-greffe utilisé par le pépiniériste. (agronomy-journal.org)

Veena and her lab develop and explore genetic engineering technologies to develop plants with improved traits. (danforthcenter.org)
This work is so exciting to me, because the moment we see a successfully genetically engineered plant through this process, it is a step forward to understand the underlying mechanism of biological processes that could eventually result in plants with improved traits that can produce more food for people or better nutrition. (danforthcenter.org)
But unlike traditional selective breeding, genetic engineering vastly expands the range of traits that can be moved into plants and enables breeders to import DNA from virtually anywhere in the biosphere. (cornucopia.org)
The present study aimed to explore the genetic variation in fodder maize landraces for various morpho-physiological traits and estimation. (researchgate.net)
This review presents genetic and molecular advances related to qualitative and quantitative phenotypic traits and biochemical compounds in melons. (bvsalud.org)

Carbenicillin Disodium Salt is a penicillin-derived β-lactam antibiotic that interferes with cell wall synthesis, primarily in gram-negative bacteria such as Pseudomonas aeruginosa , while showing low toxicity to plant tissues. (agscientific.com)
Plants, algae, and some bacteria make their own food through photosynthesis, while animals and most other organisms obtain food by consuming other organisms or organic matter. (newideas.net)
be able to describe molecular genetic processes in bacteria. (lu.se)

Abstract Salt stress is one of the most serious abiotic stresses limiting plant growth and development. (techscience.com)

This enabled huge advances in medical science and diagnostics, and determining the sequence of the N. benthamiana genome has the potential to similarly aid biotechnological and agricultural research and plant-based production of vaccines and therapeutics. (lincoln.ac.nz)

Daniell H, Singh ND, Mason H, Streatfield SJ (2009) Plant-made vaccine antigens and biopharmaceuticals. (springer.com)
The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)

Parrou, J.L.: Genetic and metabolic regulation of glycogen and trehalose in Saccharomyces cerevisiae. (tu-dresden.de)

Frontiers in Plant Science, 2017 8, 271. (ac.ke)

To address the long juvenile phase of fruit trees, the book includes a chapter on plant breeding technique that can significantly shorten the breeding periods. (intechopen.com)
The application of genetic engineering to agriculture builds on the ancient practice of selective breeding. (cornucopia.org)
He identified several microRNAs for genetic and breeding purposes. (icac.org)
After finishing my undergraduate studies at Lund, I worked at biotech labs in plant breeding companies for almost 15 years. (lu.se)

This bacterium induces the neoplastic growth of plant cells that differentiate to form "hairy roots. (bioone.org)

Genetic Transformation Systems in Fungi. (lu.se)

Genetic engineering offers potentially viable solution to look for alternatives beyond Bt toxins with similar pattern of toxicity. (intechopen.com)
In their research, genetic engineers continually encounter unintended side effects - plants create toxins, react to weather differently, contain too much or too little nutrients, become diseased or malfunction and die. (cban.ca)
They noted that genetic transformation has the potential to produce unanticipated allergens or toxins and might alter the nutritional quality of food. (cornucopia.org)

Several citrus transformation protocols have been previously published. (springer.com)
Transformation protocols and retrieval of recombinants are essential to the success of gen. (intechopen.com)

The Plant Transformation Facility was recognized as a world leader in transformation of corn and soybeans and this facility served over 200 research groups from 125 institutions and 17 countries. (sivb.org)

1995. Cytochrome P4501A1 and glutathione S-transferase (M1) genetic polymorphisms and post menopausal breast cancer risk. (cdc.gov)

Experiments were conducted to develop an efficient and reliable protocol for in vitro direct regeneration of potato for future transformation. (banglajol.info)
½MS medium supplemented with 0.5 - 1.0mg/l IBA resulted the best performance as to the rooting from ZR treated regenerated shoots having 100% plant survival at ex vitro conditions. (banglajol.info)
To obtain plant material in reported methods for Cumin regeneration, seeds have to be germinated in vitro and then hypocotyls and cotyledon leaflets of seedlings used as explant. (scialert.net)
An interesting chapter is dedicated to in vitro fig regeneration and transformation systems. (intechopen.com)
Application of genetic engineering in improving many maize genotypes is, however, limited by lack of suitable regeneration systems that result in normal and fertile plants from the in vitro grown transformed cells. (scialert.net)

Production of this plant is limited due to several biotic stresses of which wilt diseases are the most serious. (scialert.net)

Since the beginning of the 90s lots of cationic plant, cysteine-rich antimicrobial peptides (AMP) have been studied. (frontiersin.org)

1. We have demonstrated that biolistic bombardment using embryogenic Vitis cultures is an effective process for genetic transformation. (cornell.edu)

The transgenic constructs used in genetic modification are basically the same whether it is of human cells or of other animals and plants. (i-sis.org.uk)

While no effective HLB control methods or HLB resistance citrus cultivars have been commercialized, transgenic plant technologies have been recognized as a powerful tool to control the HLB disease. (springer.com)
Herbicide resistance is the main characteristic that the biotechnology industry has chosen to introduce into plants. (cornucopia.org)
Genetic resistance is the most feasible approach to combat losses caused by viral diseases. (researchgate.net)

It has also been shown that the kn1 mRNA can be transported bidirectionally via phloem cells in plants (Duan et al. (springer.com)
Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
Transformation of human breast epithelial cells by chemical carcinogens. (cdc.gov)
Malignant transformation is the complex process by which cancerous cells develop from healthy cells. (msdmanuals.com)

Photosynthesis, the natural process all plants use to convert sunlight into energy and yield, is a surprisingly inefficient 100+ step process that RIPE researchers have been working to improve for more than a decade. (agrimarketing.com)
These factors allow researchers to go from genetic transformation to a field trial within months. (agrimarketing.com)

A. tumefaciens cell density OD660 = 0.8 for inoculation, half an hour infection period, and three days of co-culture duration were found to be optimal for callus transformation. (vtt.fi)
Hairy roots are induced by the incorporation of a bacterial-derived segment of DNA transferred (T-DNA) into the chromosome of the plant cell. (bioone.org)
1987 Invited Speaker, NATO Advanced Study Institute on Plant Cell Biotechnology, Portugal. (cornell.edu)
1980-present Ad hoc Reviewer: JASHS, HortScience, Plant Cell Rept. (cornell.edu)
Plant Cell 2012, 24(7):3153--‐66. (ac.ke)
I still plan to complete and finish one of my projects, in which cell culture and genetic transformations of clover are developed and used in order to enhance the nodulation efficacy. (lu.se)
Transformation assay of established cell lines : mechanisms and application, proceedings of a workshop organized by IARC in collaboration with the US National Cancer Institute and the US Environmental Protection Agency, held in Lyon, 15-17 February 1984 / editors, T. Kakunaga, H. Yamasaki. (who.int)
The first step in cancer development is initiation, in which a change in a cell's genetic material (a mutation) primes the cell to become cancerous. (msdmanuals.com)

It takes several minutes for the plant to switch off the protective mechanism, costing plants valuable time that could have been used for photosynthesis. (agrimarketing.com)

Genetically modified organisms (GMOs) are organisms whose genetic material has been artificially modified to change their characteristics in some way or another [ 1 ]. (hindawi.com)

I am also interested in the specific proteins, pathogesis-related proteins, expressed by plants as a response to microbial colonizations or attacks. (lu.se)

Genetic transformation provides scientists with many plants that are potentially useful for basic and applied research, but a significant amount of time and effort is needed to sort through them to find those with only one copy of the added DNA. (usda.gov)

This review details the anatomy, development, physiology, and molecular characteristics of the Lemnaceae to introduce them to the broader plant research community. (osti.gov)
The Department of Plant Pathology at the University of Nebraska-Lincoln (UNL) has a long tradition of excellence in research and service to the state,the nation, and internationally. (unl.edu)
Their paper has just been published in Nature Plants and on a website providing complete access to the genome and its annotation, which will assist the global scientific community's research into the plant. (lincoln.ac.nz)
Since 2021, Dr. Wang has served at the National Science Foundation as Program Director for Plant Genome Research Projects, and, in this role, she leads a team to determine the types of research that is prioritized for funding and establishes the research agenda for the call for proposals. (sivb.org)

The goal of our lab is to develop technologies that make plant transformation faster, more cost efficient and more precise. (danforthcenter.org)
Implementation of efficient marker-free system to meet commercial plant transformation demand. (danforthcenter.org)

Dr. Wang was then appointed as the Director of the newly founded Plant Transformation Facility in the Department of Agronomy at Iowa State University. (sivb.org)

Anatomy29

Organisms34

Diseases3

Chemicals and Drugs42

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Psychiatry and Psychology1

Phenomena and Processes62

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture12

Information Science4

Health Care4

Agrobacterium14

Transgenic plants11

Genes19

Gene21

Species15

Efficiency6

Growth regulators4

Genomic5

Molecular Biology4

Tissue4

Regulators2

Biology5

Crops7

Explants3

Cauliflower mosai1

Genetics7

Improvement8

Conventional3

Diversity5

Crop3

Soybean3

Tobacco4

Populations3

Drought2

Abiotic and biotic1

Recombination1

Medicinal3

Microorganisms5

Proteomics1

Varieties3

Crown gall3

Traits5

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Abstract1

Agricultural1

Antigens2

Regulation1

Frontiers1

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Toxins3

Protocols2

Corn1

19951

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