Physiological conditions cell culture techniques. Medical search. Frequent questions

regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. (benthamscience.com)
Gelomics fast tracks biomedical research and development by enabling researchers to grow human-like tissues in the laboratory that drastically improve the translational value of cell-based research and reduce the requirements for animal experimentation. (bio-itworld.com)
Gelomics' first product series, LunaGel™ is a photocrosslinkable cell culture system that recreates the natural extracellular matrix (ECM) surrounding cells in the human body, allowing researchers to grow three-dimensional microscopic tissues, rather than just cells on plastic surfaces. (bio-itworld.com)
Finally, we compared the phase shift and the period change resulting from two methods of light stimulations of retinal explants: the first involved the transfer of the cultured tissues from the Lumicycle into a light stimulation chamber, while the second used a light delivery apparatus embedded in the Lumicycle. (molvis.org)
Spheroids and complex organoid models allow the formation of self-assembled cell aggregates recapitulating the cellular organization and functionality of specific tissues or organs 8-10 . (jneurology.com)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
these systems integrate engineered human tissues into physiological microenvironment supplied by a vasculature-like microfluidic perfusion. (nature.com)
Summary/Conclusion: These final results might indicate (i) feasibility of genetic modifications of EVs enforcing their regenerative proprieties as well as (ii) enhanced action of EVs from hiPS cells overexpressing miR1, miR199a and miR126 in regeneration of ischaemic tissues. (translateinthetownships.com)
Traditional metabolic and physiological measurements are combined with molecular analyses in various tissues as well as in primary cell cultures. (varbi.com)
Improved models based on human cells and tissues are urgently needed to understand pathogenesis as well as to explore novel therapeutic strategies. (biomedcentral.com)
With these environmental cues in mind, biologists have developed more physiologically accurate methods for culturing cells and tissues, including new media formulations and co-culture techniques. (mimetas.com)
They demonstrated that, in cultured mouse cortical neurons, addition of zinc at a focus similar compared to that within ischemic human brain tissues created significant neuronal damage. (ole2011.org)
Compared with non-targeted nanoparticles, C@CF/CaP could specifically target tumor tissues and then enter tumor cells through folate receptor-mediated endocytosis. (bvsalud.org)

The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Cell culture systems are an important in vitro tool in basic research and essential in drug discovery and development. (jneurology.com)
Since that time, cell culture methods have been greatly advanced, and the two-dimensional (2D) cell culture systems evolved to be the standard in vitro tool. (jneurology.com)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
In vitro investigation of human umbilical vein endothelial cell (HUVEC) and adipose-derived stem cell (ADSC) responses in a biomimetic 3D bioprinted blood vessel model under complex physiological flows was performed. (aps.org)
We use cell culture techniques, mutagenesis approaches, primary cultures of neurons and glia, pharmacology, in vitro models of stroke and epilepsy, and in vivo models of neuropathic pain and cocaine abuse disorder. (drexel.edu)
The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
Concurrently, in vitro scientific studies were carried out to examine the responses of iPSCderived human cardiomyocytes to plasma-derived EVs applying BNP being a surrogate marker of the cardiovascularIntroduction: Extracellular vesicles (EVs) represent population of tiny circular membrane vesicles secreted by most cells such as stem cells (SCs). (translateinthetownships.com)
Cardiac cells and endothelial cells have been utilised as target cells in vitro, and their functional properties were evaluated after hiPSEVs treatment. (translateinthetownships.com)
The hiPS-EVs enhanced also proangiogenic capacity, migration and metabolic activity of HCAEC cells in vitro. (translateinthetownships.com)
Tissue culture, also known as micropropagation or in vitro propagation, is a method of growing plants in a sterile, controlled environment using small tissue samples from mother plants. (plantcelltechnology.com)
Along with microfluidic or organ-on-a-chip systems , 3D cell culture offers researchers an unparalleled ability to recreate physiological compositions and spatial arrangements of cells in vitro . (mimetas.com)
Interferon (IFN)-γ synthesis by T cells after an in vitro. (cdc.gov)

Other compounds that enhance the activity of EAAT2 (the main astrocytic transporter in the CNS) in pathological conditions, such as ß-lactam antibiotics, attenuate damage in both acute and chronic neurodegenerative disorders. (drexel.edu)
Apoptosis is a programmed cell death process under both normal physiological and pathological conditions. (edu.pl)

The introduction of cell culture techniques has helped a lot in understanding the physiological processes like cell signalling, neurobiology, cell proliferation, pathogenesis of diseases, apoptosis and even more. (benthamscience.com)
Microparticles are extracellular vesicles shed by cells upon activation or apoptosis involved in physiological processes such as coagulation and inflammation. (unipi.it)
Describe the structure of an eukaryotic cell, and the central processes in cell division. (ntnu.edu)
These 2D cell culture systems have already provided many insights into basic cellular functions, biological mechanisms and various disease processes. (jneurology.com)
Models of animal cell culture systems have a wide range of potential applications, such as analysis and prediction of experimental results, optimisation of culture conditions for prolonged viability, and perhaps most importantly, the investigation of fundamental metabolic processes and their subsequent elucidation. (imperial.ac.uk)
The integration of these three research tools represents a unique, novel, and interdisciplinary approach to addressing the complicated research and industrial problem of model-based control and optimisation of mammalian cell culture processes. (imperial.ac.uk)
Since intercellular communication seems to play a role in various biological processes, it might be important to conduct studies in co-culture systems, which at least mimic partially more physiological conditions, and enables this intercellular exchange to occur. (intechopen.com)
This article discusses the challenges facing cell therapy research, and how the latest cell culture technologies are delivering enhanced safety capabilities, improved laboratory performance and, ultimately, optimised cell therapy processes. (ddw-online.com)
Because of the huge potential for variability within treatments, an additional challenge in cell therapy is the need for robust manufacturing processes that consistently deliver safe and effective products. (ddw-online.com)
Cell therapy manufacturing processes can range from the simple expansion of autologous cells that will be administered back to patients, to the complex genetic manipulation of allogeneic cells that could be stored and banked for the treatment of multiple patients. (ddw-online.com)
The regulations surrounding cell therapies must therefore be sufficiently broad to cover this wide range of therapeutic processes, while still safeguarding product safety and quality. (ddw-online.com)

This led to the basis of cell culture studies, where cells grown in controlled artificial conditions simulate the conditions prevailing in natural ones, therefore, presumed to act as those in in vivo conditions. (benthamscience.com)
Ex vivo cardiac function is evaluated using an isolated perfused rodent heart apparatus that allows for the quantification of cardiac pressures, volumes, and flow under controlled conditions. (unco.edu)
For physiologically relevant disease modeling, three-dimensional (3D) cell culture systems are of great importance because they provide a more representative in vivo -like micro-environment to the cells. (jneurology.com)
However, 2D cell cultures do not represent the physiological in vivo microenvironment of the cells 2 . (jneurology.com)
Usually, a homogenous cell population is cultured as a monolayer, while in vivo the cells are interacting with a heterogenous cell population. (jneurology.com)
These different 3D cell culture systems provide a more representative in vivo -like microenvironment which influences cellular features like morphology, proliferation, differentiation and migration 11,12 . (jneurology.com)
Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (sigmaaldrich.com)
The expected in vivo chemical stability of the CaP coating was measured by immersing CaP-coated PCU discs in cell culture media under physiological conditions. (omicsonline.org)
Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
Physiological osmolality (290 mOsm/kg) is recommended for in vivo applications. (sigmaaldrich.com)
Disease models for GERD, BE, GVHD, and ulcerative colitis based on three-dimensional human cell and tissue culture systems that recapitulate in vivo growth and differentiation in inflammatory-associated microphysiological environments would enhance our understanding of disease progression and improve our ability to test for disease-prevention strategies. (biomedcentral.com)
We combine experiments in cultures and in vivo with theory and computer simulations. (nyu.edu)
Cells in vivo experience a complex, three-dimensional environment that exposes them to circulating molecules, neighboring cells, and the extracellular matrix (ECM). (mimetas.com)
Meanwhile, 3D cell culture systems feature increased complexity for increased faithfulness to the in vivo environment. (mimetas.com)
While WA/1 virus exhibited a moderately increased proportion compared to that in the inoculum following co-infection in human respiratory cells, Delta variant possessed a substantial in vivo fitness advantage as this virus becoming predominant in both inoculated and contact animals. (cdc.gov)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)

The researchers optimized a previously developed culturing approach known as the air-liquid interface technique so that it would more closely simulate the physiological conditions found in the lung airway. (eurekalert.org)
Numerous prior studies have investigated the dissolution of CaP coatings, such as hydroxyapatite, in conditions designed to simulate physiological conditions, i.e. typically at pH 7.4 and at ~37 °C [ 3 - 7 ]. (omicsonline.org)

Conventional DNA origami assembly requires levels of magnesium easily 10-30 times as high as those in normal physiological conditions. (aalto.fi)

We focus on innovations for the generation of neurons, astrocytes, oligodendrocytes, microglia as well as endothelial cells and pericytes. (jneurology.com)
Here, we refine these observations and show that glutamate produces the following three distinct optical responses in mouse primary cortical neurons in culture, predominantly mediated by NMDA receptors: biphasic, reversible decrease (RD) and irreversible decrease (ID) responses. (jneurosci.org)
In this study, we have taken advantage of the possibility of DHM to monitor transmembrane water fluxes as detected by the phase signal, to assess the activity of NKCC1 and KCC2 in response to depolarizations induced by glutamate on primary neurons in culture. (jneurosci.org)
During a sensory stimulus, the responses of neurons depend on the patterns of connections between excitatory and inhibitory cells and on their intrinsic and synaptic properties. (nyu.edu)

Scientists at the Dana-Faber Cancer Institute believe that those odds can be shortened by taking into account neighbouring cells in the tumour microenvironment in preclinical testing. (drugdiscoveryopinion.com)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)
Cancer cells, including leukemia cells, reside in a complex microenvironment, which influences biology and activity of the cells. (intechopen.com)
The present chapter outlines the importance of modern, multiparameter flow cytometry methods, which allow to analyze interactions between different types of cells within the leukemia microenvironment. (intechopen.com)

Poly (ADP-ribose) polymerase 1 is a mediator of programmed-necrotic cell death and appears to be also involved in the apoptosis. (edu.pl)
5] Elmore S. Apoptosis: a review of programmed cell death. (edu.pl)
Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor. (edu.pl)
Success: Our information indicate that hiPS-EVs carrying procardio- and proangiogenic miRNAs may perhaps defend cardiac cell types from apoptosis at the same time as enhance their proliferation, metabolic activity, migration and cardiomyogenic differentiation. (translateinthetownships.com)

The survival of Enterococcus faecalis under different environmental stresses (salinity, starvation and sunlight) were examined under controlled conditions in the laboratory. (iwaponline.com)
We found that exposure to the SARS-CoV-2 spike protein alone was enough to change baseline gene expression in airway cells," said Nicholas Evans, a master's student in the laboratory of Sharilyn Almodovar, PhD, at the Texas Tech University Health Sciences Center. (eurekalert.org)
Describe the basic principles for isolation and growing microorganisms in the laboratory, including the composition of culture media. (ntnu.edu)
Although both non-malignant cells and secreted proteins from tumour and stromal cells are recognised to be active participants in cancer progression, early laboratory testing is typically carried out using homogeneous populations of tumour cells. (drugdiscoveryopinion.com)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Our group established the first laboratory cultures of phototrophic consortia that represent the only laboratory model system currently available for the study of highly specific interactions between nonrelated bacteria. (dsmz.de)
Lastly, laboratory cultures of phototrophic consortia also represent valuable model system for establishing suitable cryoconservation techniques for complex microbiomes. (dsmz.de)
Setting up a tissue culture laboratory requires specialized equipment, sterile conditions, and skilled personnel. (plantcelltechnology.com)
Successful tissue culture implementation requires expertise in plant tissue culture techniques and laboratory practices. (plantcelltechnology.com)

Rousselot Biomedical will supply Gelomics with its X-Pure GelMA (gelatin methacryloyl), a photo cross-linkable extracellular matrix, for use in Gelomics' LunaGel™ 3D Tissue Culture System. (bio-itworld.com)
In addition, in physiological conditions the cells are interacting with multiple extracellular matrix (ECM) components, which can actively affect the behavior of the cells 3 . (jneurology.com)
The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)

We are studying the interactions between the amnion cells and immune cell subsets as well as endogenous stem cell populations under different inflammatory conditions during development as well as adulthood. (hudson.org.au)
Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)
The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)
In this respect, different routes of interactions were defined, such as direct cell-cell interactions or indirect cross talk, by release of soluble factors or vesicular particles containing proteins, RNAs and other molecules. (intechopen.com)
Thus, it is crucial to improve analytical methods of investigation of co-cultured cells, to study their interactions and so to understand biology of leukemia in order to understand molecular mechanisms and offer novel therapeutic strategies. (intechopen.com)
This will not only improve our understanding of the physiological basis of interactions in cyanobacterial co-cultures, but also will add value to the cyanobacteria collection and guide subsequent attempts to obtain pure (axenic) cultures. (dsmz.de)
They are ideal for the study of complex physiological interactions, cell signaling, and host pathogen defense. (jove.com)
Features of the acoustic space are represented in cortex through the complex interactions between excitatory and inhibitory cells within and across layers. (nyu.edu)
3D cell culture represents yet another step towards this goal by focusing on mimicking normal cell-matrix and cell-cell interactions. (mimetas.com)
In multicellular structures, cell-cell interactions dominate. (mimetas.com)

The authors suggest that the shape and movement of cells in 2-D culture experiments are artifacts of the environment and could produce misleading results in studies to test the effects of drugs on cell motility. (drugdiscoveryopinion.com)
The focal adhesion proteins still modulate cell motility, but not in the same way as in a 2-D environment. (drugdiscoveryopinion.com)
Because loss of adhesion and increased motility are hallmarks of cancer cells, it is important to understand cell motility under physiological conditions and to use culture techniques that most closely mimic this. (drugdiscoveryopinion.com)
The primary changes to cells in 3D are rather obvious - shape, motility, and polarity are all affected - but can have important secondary, less-obvious tissue-specific consequences. (mimetas.com)

In a second set of experiments, we quantified the effect of a medium change done after 4, 6, 8, 9, or 10 days of culture on the phase and period of retinal explants. (molvis.org)
Role of microbes in biogeochemical cycles Physiological experiments and quantitative microbial analysis. (ntnu.edu)
Cell culture experiments to screen for compounds that can inhibit cell migration - and potentially metastasis of cancer cells - are typically carried out in a 2-D environment, but researchers at Johns Hopkins University and the University of Washington suggest that results from such experiments may be, at best, misleading. (drugdiscoveryopinion.com)
This may explain why positive results from cell culture experiments do not always translate into efficacy in animal models. (drugdiscoveryopinion.com)
A systematic approach has therefore been pursued in which a new technique has been developed that yields useful information on the ease of estimation of these parameters from potential experiments. (imperial.ac.uk)
For this reason, they are extensively used in a wide range of applications from protein production to bacterial biofilms and endothelial cell experiments. (imperial.ac.uk)
The expertise of IGEOMAR includes all culture techniques for marine fungi and bacteria up to 250 Liter scale, isolation and molecular identification of microorganisms, functional gene analysis, physiological experiments to specifically trigger and optimize secondary metabolite production, chemical analysis of the cell extracts and identification of their chemical structures (HPLC-UV/MS, HPLC/MS-MS, FCPC, NMR) and bioassays (antibacterial, antifungal, antiproliferative) to determine the application potential of metabolites. (marinefungi.eu)

It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. (heraldopenaccess.us)

Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
Especially in drug discovery and development, 2D cell culture systems are essential concerning compound testing and high-throughput screening (HTS) assays. (jneurology.com)
In drug discovery and development, 3D cell culture systems are more frequently used in HTS assays 13,14 . (jneurology.com)
More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)

Different decay kinetics were observed for measurements of the bacteria using a culture-based method and a molecular technique (PMA-qPCR). (iwaponline.com)
When E.Coli bacteria were introduced into the air on the 'lung' side of the chip, white blood cells on the 'blood' side of the chip migrated through the porous membrane into the air chamber to destroy the bacteria. (drugdiscoveryopinion.com)
Flow cytometry offers multiparameter analysis of bacteria on a cell-by-cell basis to characterize populations. (purdue.edu)
1993. Viability and isolation of marine bacteria by dilution culture: Theory, procedures and initial results. (purdue.edu)
Sincle cell genomic analysis of the bacteria constituting different types of phototrophic consortia indicate a long-term co-evolution of the partners. (dsmz.de)
The E. coli bacteria will initially be cultured in a lab setting prior to filling one section of the Type #3 FME Mini-Lab. (ncesse.org)
Once the FME Mini-Lab is returned, living bacteria will be examined in a viable cell count technique. (ncesse.org)
Development of a method for bacteria and virus recovery from heating, ventilation, and air conditioning (HVAC) filters. (cdc.gov)
Our results indicate that the airborne concentration of spore-forming bacteria in building AHUs may be determined by analyzing the material collected on HVAC filter media, however culture-based analytical techniques are impractical for virus recovery. (cdc.gov)

An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
Irving, United States, 16th November 2022 / Sciad Newswire / Darling Ingredients' Health Brand, Rousselot, the global leader in collagen-based solutions, and Gelomics, the world-leading provider of fully integrated 3D cell, organoid and tissue culture technologies, have entered into a cobranding partnership. (bio-itworld.com)
GelMA is a well-known biomaterial with tuneability and bioactivity that is used in a wide range of tissue engineering, 3D bioprinting and 3D cell culture applications. (bio-itworld.com)
Founded in 2018 in Australia, Gelomics has rapidly become a world-leading provider of fully integrated 3D cell, organoid and tissue culture technologies. (bio-itworld.com)
Gelomics is a world-leading provider of fully integrated 3D cell, organoid, and tissue culture technologies. (bio-itworld.com)
Applications of complex 3D tissue-like models in physiological and pathological studies. (ibecbarcelona.eu)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
The successful candidate will work primarily with cell models, but will also use skeletal muscle tissue from humans and mice. (varbi.com)
Infiltrating immune cells and stromal components of tissue including fibroblasts contribute to dynamic changes occurring in tissue related to disease development. (biomedcentral.com)
Then, filter the tissue through a 100 micrometer cell strainer and discard the flow through. (jove.com)
Immuno-oncology therapies typically use genetically modified T-cells to combat and kill cancerous tissue by identifying the cancer cells as a foreign body in need of attack. (ddw-online.com)
One such technique that holds great promise for the cannabis industry is tissue culture. (plantcelltechnology.com)
While tissue culture has been widely used in the horticultural and agricultural industries, its application to cannabis cultivation is relatively new but rapidly gaining attention. (plantcelltechnology.com)
Tissue culture offers a means to preserve the genetic integrity of cannabis strains. (plantcelltechnology.com)
Through tissue culture, cultivators can create a "bank" of genetic material, ensuring the preservation of valuable and rare strains. (plantcelltechnology.com)
Tissue culture techniques involve the use of sterilized media, preventing the introduction and spread of pathogens and pests. (plantcelltechnology.com)
Although tissue culture techniques aim to maintain genetic stability, there is a possibility of somaclonal variation, which refers to genetic changes occurring during tissue culture propagation. (plantcelltechnology.com)
The legal and regulatory landscape surrounding tissue culture in cannabis cultivation varies across different jurisdictions. (plantcelltechnology.com)
Cultivators must ensure compliance with relevant regulations and obtain necessary permits before implementing tissue culture techniques. (plantcelltechnology.com)
Collaboration between industry stakeholders and regulatory bodies is crucial to facilitate the integration of tissue culture into the cannabis sector. (plantcelltechnology.com)
Collaboration with academic institutions and research organizations can help bridge the knowledge gap and promote innovation in tissue culture methods. (plantcelltechnology.com)
Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook. (mimetas.com)
1994). This pattern of localized toxicity emphasizes the impor- inhaled organic acid vapor, acrylic acid, than the olfactory epi- tance of local tissue dose for interspecies extrapolation and risk thelium of the rodent nasal cavity when the exposure conditions assessment. (cdc.gov)

Our group combines clinically relevant physiological outcomes with unique 3D co-cultures and cutting edge molecular biology techniques to answer some of the most pressing questions when translating bench based stem cell research to clinical utility. (hudson.org.au)
The study is published in Nature Cell Biology . (drugdiscoveryopinion.com)
In-depth models can be utilised as tools in predictive cell biology. (imperial.ac.uk)
It is clear that the biological behaviour of cells is of such complexity that any model, to be robustly predictive, must be sufficiently structured and detailed in terms of the captured biology. (imperial.ac.uk)
The DNA origami technique is a widely used method for making complex, yet well-defined nanostructures, with applications in biophysics, molecular biology, as well as drug and enzyme delivery. (aalto.fi)
Therefore, we are looking for highly motivated students with a strong background in cell biology and a genuine interest in skeletal muscle physiology. (varbi.com)

Current understanding of mammalian cell cultures lies in the association of their metabolic behaviour to their antibody production output. (imperial.ac.uk)
Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)
Secondary metabolite production is studied under a variety of metabolic conditions and stimulated experimentally to enlarge the metabolite profile of fungal strains. (marinefungi.eu)

In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)
T-cell therapies based on chimeric antigen receptor (CAR) T-cells are widely employed for this purpose, as they have the capacity to proliferate within the patient after infusion and offer sustained functional immunity. (ddw-online.com)
Using a rapid flow cytometric test, we previously showed that monitoring interferon (IFN)-γ production by antigen-pulsed T cells provides a quantitative and functional assessment of HIV- or cytomegalovirus (CMV)-specific CD8(+) and CD4(+) T cells ( 4 - 6 ). (cdc.gov)

Nevertheless, together with benchmarking analyses, we show here that the diametric ratio approach can be applied for evaluating the physiological conditions experienced by microbes in situ. (osti.gov)

This technology enables the differentiation of the brain cell types of interest, also from diverse patient-derived material. (jneurology.com)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
The goal is to characterize the dynamic flow conditions which drive endothelial cell mechanotransduction, cellular growth, and morphological maturation, as well as the differentiation of ADSCs into endothelial-like phenotypes. (aps.org)
Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
How Does Microgravity Affect the Differentiation of Mesenchymal Stem Cells and the Function of Osteoblasts and Osteocytes in Producing Bone Matrix? (ncesse.org)
To facilitate the creation of substances that inhibit the progression of the condition, we must understand the role of gravity in regulating the differentiation of mesenchymal stem cells and osteoblast performance. (ncesse.org)
These newly available materials led to compelling evidence that the physical cellular environment is far from inert, but acts as a biological and mechanical signal to regulate cell-type specific function, differentiation, and morphology 1 . (mimetas.com)

Sterile pyuria is urine which contains white blood cells while appearing sterile by standard culturing techniques. (wikipedia.org)
After finishing this course, the students can: - Master basic microbiological techniques (e.g. sterile techniques, microscopy, traditional microbial tests). (ntnu.edu)
Cylindrical vessel scaffolds were 3D printed using polylactic acid (PLA) material and the vessel lumen were inoculated with a co-culture of HUVEC and ADSC in a custom-built bioreactor system using cell growth medium ((DMEM, Corning®) and (ECM, Cell Applications Inc)). The vessels were installed in a sterile experimental flow loop facility capable of providing physiological flow forcing conditions. (aps.org)

Even in cell culture systems designed to more closely mimic a 3-D environment, the cells may be only partially embedded in a matrix and produce misleading results. (drugdiscoveryopinion.com)
Spaceflight osteopenia, a condition that closely mimics earth based osteoporosis, causes bone density to decrease at an elevated rate. (ncesse.org)
The more closely a cell culture system can replicate those conditions, the more closely those cells will replicate the behaviors and responses of cells in the body. (mimetas.com)

The generation and maturation of high-fidelity organoids entails developing and optimizing environmental conditions to provide the optimal cues for growth and 3D maturation, such as oxygenation, mechanical and fluidic activation, nutrition gradients, etc. (mdpi.com)
These 3D cell culture systems range from scaffold and scaffold-free techniques to more complex systems like organoids. (jneurology.com)
Enteroids are three dimensional organoids derived from intestinal epithelial cells. (jove.com)
Since then, remarkable progress has been made in 3D cell culture, including organoids, miniature organ-like structures that self-organize in 3D culture. (mimetas.com)

Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)
The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)

The "Biomimetic systems for cell engineering" Group is looking for a young researcher on the development of hydrogel-based microfluidic toolbox to investigate immune cell recruitment in colorectal cancer. (ibecbarcelona.eu)
This review focuses primarily on recent advances in biologically inspired microfluidic systems that are based on 3-dimensional hydrogel cell cultures and discusses advantages and current challenges, various applications of microfluidic hydrogel cell cultures and future perspectives. (heraldopenaccess.us)
These microfluidic cell cultures are considered potential candidates to provide next generation cell analysis systems. (heraldopenaccess.us)
Starting from single cell analysis using miniaturized flow cytometers [3] a variety of microfluidic devices have been developed for cell studies to investigate cell transport and cultivation in the absence and presence of concentration and temperature gradients or shear force conditions. (heraldopenaccess.us)
The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. (heraldopenaccess.us)

Molecular-based identification techniques such as PCR could be used. (cdc.gov)
Fluorescent Protein Technology - It took over thirty years, and the advent of recombinant DNA as well as vastly improved molecular biological approaches to see the pioneering work of Osamu Shimomura developed into a useful tool for live-cell imaging by Doug Prasher and Martin Chalfie. (fsu.edu)

The development of physiologically relevant, human cell-based culture systems is therefore a major focus of our research. (biomedcentral.com)

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. (jneurosci.org)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)

Leveraging the consistency and purity of X-Pure GelMA, the LunaGel X-Pure products offer Gelomics' customers an easy-to-use solution for creating the optimal experimental conditions for cell culture. (bio-itworld.com)
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. (sigmaaldrich.com)
There are a number of complications that make it a difficult process to ensure that the culture is growing under optimal conditions at all times. (imperial.ac.uk)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
Hypoxia maintains the niche where stem cells stay in optimal condition that benefits the. (globalspec.com)

The researchers found that cultured human airway cells exposed to both low and high concentrations of purified spike protein showed differences in gene expression that remained even after the cells recovered from the exposure. (eurekalert.org)
Microfluidics also enables precise regulation of soluble factors including drug candidates, growth factors at specific solution concentrations and gradients, thus providing robust and reproducible measurement conditions. (heraldopenaccess.us)
Until now, the technique has required high concentrations of magnesium well above those found in the human body. (aalto.fi)
Despite these known facts, there is no direct proof showing that TRPM7 stations are likely involved in intracellular zinc dynamics at physiological/pathological relevant concentrations and moreover, in zinc-mediated neurotoxicity. (ole2011.org)

The rapid expansion in biomedical research using live-cell imaging techniques over the past several years has been fueled by a combination of events that include dramatic advances in spinning disk confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. (fsu.edu)

Since the mammalian cells do not uptake the foreign DNA efficiently, the availability of effective methods for introducing genes into the cells is essential. (benthamscience.com)
The difference between the two enumeration methods (i.e. the VBNC cells) was modelled as a function of environmental variables. (iwaponline.com)
Methods: Cells were isolated from the blood and the umbilical cords of normal donors with standard techniques and cultured in the presence of particulate from a standard reference. (unipi.it)
3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
Allogeneic methods are more akin to the pharmaceutical model of drug manufacturing as products can be produced on a larger scale and can be prepared for 'off the shelf' distribution, provided the recipients have an immunological profile matching the cells or cell products. (ddw-online.com)
Utilizing a mix of fluorescent zinc imaging, steel response element-based reporter gene assay, cell damage analysis and little interfering RNA methods, Inoue and co-workers were the first ever to provide a solid proof helping that TRPM7 stations represent a book pathway for intracellular zinc deposition and zinc mediated neurotoxicity [50]. (ole2011.org)

The team are now exploring whether the system can mimic gas exchange between alveolar cells and the bloodstream and believe that the device provides proof-of-principle for the concept that organs-on-chips could replace many animal studies in the future. (drugdiscoveryopinion.com)

The outcomes from these studies will inform the design of clinical trials and allow us to develop target approaches for the clinical use of amnion cells. (hudson.org.au)
The work will also foster the development of novel therapeutic and preventive strategies that will improve patient care for these important clinical conditions. (biomedcentral.com)

Cellular co-culture was subjected to wall shear stresses from physiological flow forcing for up to 8 hours each day and then followed by steady flow. (aps.org)

The main goals of my research are twofold: to investigate the regulation of EAATs in physiological states and neurological disorders, and to progress the development of compounds that target these transporters for therapeutic purposes. (drexel.edu)

This observation is of particular interest since it shows that DHM is the first imaging technique able to monitor dynamically and in situ the activity of these cotransporters during physiological and/or pathological neuronal conditions. (jneurosci.org)
Even more relevant to human brain ischemia, Zn2+-mediated neuronal injury in OGD conditions was reduced by TRPM7 knockdown [50] also. (ole2011.org)

Resulting data, used in conjunction with other techniques, have improved understanding of these organisms and their function. (purdue.edu)

This phenomenon suggests the entry of the cells into another physiological state i.e. the viable but non-culturable (VBNC) state. (iwaponline.com)
These innovative therapies rely on the use of techniques and technologies that minimise the risk of contamination and achieve the strictly-controlled, secure environment necessary for growing healthy, viable cells. (ddw-online.com)

show this method can be used for examining other physiological conditions, such as exposure to nitric oxide and osmotic pressure. (osti.gov)

Post-facto analysis was performed on dissected vessels using confocal microscopy techniques, live/dead staining, and polymerase chain reaction (PCR) testing to probe cellular growth and proliferation indicators. (aps.org)
Involving the direct use of whole cells or cellular material in patients, cell therapy is an umbrella term for many subdivisions of biological research, encompassing fields such as stem cell therapy, gene therapy and immunotherapy. (ddw-online.com)

In this chapter, we will provide general overview of various technical approaches for detecting apoptotic cells. (benthamscience.com)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
There are two prominent approaches to modelling cells. (imperial.ac.uk)
Approaches: We obtained informed consent and recruited 24 age- and sex-matched response stable CML individuals both off-TKI (median 32.26 months, n = 6) or on long-term remedy with imatinib, nilotinib or ponatinib (median 79.01 months, n = 6/group), and assayed plasma-derived EV-associated miRNAs using the nCounterAnalysis Technique. (translateinthetownships.com)

Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
Cell-secreted vesicles have emerged as an integral com- ponent of intercellular exchange of information (Fig. 1). (unicyte.ch)

They were able to confirm that the optimized cell culture approach reflected what occurs in patients, making it useful for future translational studies. (eurekalert.org)
Our projects include basic research programs to further our knowledge of glutamate transporters regulation in physiological and disease states, and translational projects involving the development of small molecule allosteric modulators of these transporters. (drexel.edu)

Nanosight analysis confirmed that upon exposure to particulate matter both cells express particles with a size range consistent with the definition of microparticles (50-100 nm). (unipi.it)
Profiles of DAPI-DNA fluorescence intensity, along with population data, are used for cell cycle analysis. (purdue.edu)
Distributions of marine isolate Cycloclasticus oligotrophus grown at two different rates (µ) under acetate-limiting conditions for cell cycle analysis. (purdue.edu)
The analysis of the intracellular distribution of PARP-1 show a signal at the nuclear level in about 75 % of the cells of DS subjects with a slight uniformly fluorescent cytoplasm. (edu.pl)
Pulse-labeling and nanoSIMS analysis showed a physiological coupling of the phototrophic and heterotrophic partner by exchange of carbon and nitrogen compounds. (dsmz.de)
This technique requires that whole proteins or selected peptide antigens are added to blood cells, allowing the simultaneous analysis of both major histocompatibility complex class I and II restricted T-cell responses ( 7 ). (cdc.gov)
Flow cytometric analysis of T-cell responses to smallpox antigens after recent smallpox vaccination and in long-term vaccinated or not vaccinated persons. (cdc.gov)

In order to further understand mechanisms that may explain the beneficial effects of exercise in cancer survivors, we utilize tumor-bearing rodent models to study the effects of exercise on various physiological systems, with a particular focus on cardiac, skeletal, and smooth muscle function. (unco.edu)
The field of 3D cell culture systems using diverse hiPSC-derived cells is growing and gets steadily advanced. (jneurology.com)
Here, we review the latest improvements of 3D hiPSC-based cell culture systems in the field of neuroscience. (jneurology.com)
To address this challenge, multiple three-dimensional (3D) cell culture systems have been developed. (jneurology.com)
Hydrogela are the most widely used systems for 3D cell culture. (sigmaaldrich.com)
Spheroid culture can be achieved in diverse environments including low-attachment microplates, bioreactors, and microfluidics culture systems. (sigmaaldrich.com)
Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)
Complications arising in these systems include the requirement to grow cultures in complex media, the lack of on-line measurements for many of the key substrates, metabolites, and products, the limited and noisy nature of much of the available experimental data and the extremely complex underlying reaction system. (imperial.ac.uk)
Our research programme sets out to integrate modelling, experiment design and validation, and control and optimisation into a single framework that would lead to increased productivity, regulated product quality, and reduced costs for mammalian cell culture systems. (imperial.ac.uk)
Importantly, the proposed experimental setups can be easily transformed to study different cell types and different biological systems. (intechopen.com)
The main advantage of this technique is that enteroids are able to recapitulate the multiple cells types of human intestinal epithelium and are able to overcome the recognized limitations of animal models and cell based systems. (jove.com)

Conclusions: Exposure of mononuclear and endothelial cells to particulate matter upregulates the generation of microparticles at least partially mediated by calcium mobilization from intracellular storage pools. (unipi.it)
Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)

Inflammation initiated by an immune response targets epithelial cells, leading to cytotoxic effects, and is a key driver of several disease conditions in the gastrointestinal (GI) tract [ 1 ]. (biomedcentral.com)

Exercises/Lab: Microscopy and microbiological techniques. (ntnu.edu)
In 3-D, the cells take on a more spindle-like appearance, with two pointed protrusions at opposite ends and focal adhesions - if they form at all - are so small and short-lived that they cannot be resolved by microscopy. (drugdiscoveryopinion.com)
Using live-cell microscopy, the team showed that, when cells are fully embedded in a 3-D matrix, focal adhesion proteins do not form aggregates, but are distributed throughout the cytoplasm. (drugdiscoveryopinion.com)
Spinning Disk Confocal Microscopy - Spinning disk microscopy has advanced significantly in the past decade and now represents one of the optimum solutions for both routine and high-performance live-cell imaging applications. (fsu.edu)

Mass Propagation: T issue culture enables the rapid multiplication of plants on a large scale. (plantcelltechnology.com)

We also utilize cell culture techniques to maintain multiple tumor cell lines (metastatic and nonmetastatic) that are used in rat and mouse tumor-bearing models. (unco.edu)

The population balance framework is used to capture cell cycle effects while a single-cell model takes account of some features of intracellular metabolism. (imperial.ac.uk)
Consequently, the phase signal provides information about cell morphology (and volume) and the intracellular refractive index whose value is related to the amount of nonaqueous material (essentially protein content) present in the cell ( Barer, 1953 ). (jneurosci.org)
Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)
The aim of the present work was to detect the intracellular distribution of PARP-1 protein using immunofluorescence techniques and the expression of PARP-1 mRNA in culture of fibroblasts of DS subjects. (edu.pl)
Recent ultrastructural analyses have provided evidence for intracellular sorting of antenna structures in the epibiont cells, which occurs exclusively when they are in the symbiotic state. (dsmz.de)
In contrast, over-expression of TRPM7 in HEK-293 cells resulted in a rise in intracellular subsequent and Zn2+ Zn2+-mediated cell damage [50]. (ole2011.org)

Our work is focused on a stem cell-like population of cells obtained from the human amnion, a fetal membrane that comprises part of the placenta. (hudson.org.au)
Our international collaborations include key scientists at A*STAR, Children's Hospital of Philadelphia and Harvard Stem Cell Institute. (hudson.org.au)

Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Mammalian cells are currently the preferred expression system for high-value bio-pharmaceutical products, in particular, monoclonal antibodies. (imperial.ac.uk)
The technique, written into an algorithm, is developed through initial application to a structured model for mammalian cells. (imperial.ac.uk)

Therefore, the use of human induced pluripotent stem cells (hiPSCs) is important to develop human brain-like models to study the interaction of different brain cell types in health and disease. (jneurology.com)

Microparticles were assessed in the conditioned medium as posphatidylserine concentration. (unipi.it)
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization. (sigmaaldrich.com)
DNA origami nanostructures (green triangles) survive although magnesium concentration is drastically decreased from fabrication conditions. (aalto.fi)
Stem cells reside in niches that have much lower oxygen concentration than many other parts of the body. (globalspec.com)
PBMC were cultured in complete Roswell Park Memorial Institute 1640 medium, 10% v/v heat-inactivated fetal calf serum, 2 mM L-glutamine, and 10 U/mL penicillin/streptomycin at a concentration of 10 6 cells/mL. (cdc.gov)
At-home technique utilizes trays and lowconcentration bleaching gels, while in-office bleaching requires higher concentration gels, under professional supervision 4 . (bvsalud.org)

However, the establishment of a standardized procedure to replicate experimental conditions and to enable meaningful comparisons between findings from different studies is still lacking. (molvis.org)

During infection, the spike proteins bind with receptors on cells in our body, starting a process that allows the virus to release its genetic material into the inside of the healthy cell. (eurekalert.org)
Animal cell technology is an area of rapid expansion and one that produces a wide range of high-value products, including vaccines, recombinant proteins, drugs for cardiovascular, respiratory and immune diseases, and monoclonal antibodies. (imperial.ac.uk)
In fact, by mass, the most abundant proteins in our bodies are ECM proteins - the nonliving material outside of our cells that provides a physical scaffold, barrier, and mechanical and biological signaling. (mimetas.com)

The researchers also compared their cultured human airway cells to studies from others where cells were collected from patients with COVID-19 infection. (eurekalert.org)
The researchers further observed that the more tightly packed the helices in their DNA objects were, the more sensitive they were to the environment in low-magnesium conditions. (aalto.fi)

Endocrine functions of white adipocytes, and other WAT-resident cells in the stromal vascular fraction, are performed by the release of a variety of adipokines (adipose-associated cytokines) which affect the functioning of the brain, liver, pancreas and immune system 9 . (nature.com)
The protective role of bone marrow stromal cells is already commonly recognized. (intechopen.com)

Christoph Meinert, CEO of Gelomics, said, "The broad uptake of modern 3D cell culture techniques in biomedical R&D has been slowed down by a lack of easy-to-use and reproducible biomaterials that enable cells to grow and behave in a physiological manner. (bio-itworld.com)
Simple, inexpensive, and reproducible, traditional or 2D cell culture is the mainstay of biological research. (mimetas.com)

Further, prior studies have generally focused on simulating physiological rather than storage conditions, meaning that the "shelf life" of CaP coatings has not been investigated adequately despite the wide range and large number of CaP-coated orthopaedic implants in existence. (omicsonline.org)

When cells are grown in 2-D, they develop broad fan-shaped protrusions called lamella along their leading edge which help them to move forward. (drugdiscoveryopinion.com)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)

The physiological conditions experienced by a microbial community can thus be inferred using meta-transcriptomic sequencing by comparing transcription levels of specifically chosen genes. (osti.gov)

Anatomy7

Organisms8

Diseases3

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes11

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science2

Health Care4

Tissues16

Vitro13

Pathological conditions2

Processes11

Vivo17

Simulate2

Normal physiological1

Neurons4

Microenvironment4

Apoptosis4

Laboratory9

Extracellular matrix3

Interactions10

Motility4

Experiments7

Monolayer cell cultures1

Assays4

Bacteria9

Tissue30

Biology6

Metabolic3

Functional4

Microbes1

Differentiation8

Sterile3

Closely3

Organoids4

Spatial2

Microfluidic5

Molecular2

Physiologically relevant1

Quantitative2

Optimal5

Concentrations4

Genetically1

Methods6

Mimic1

Clinical2

Shear1

Neurological1

Neuronal2

Organisms1

Viable2

Osmotic pressure1

Cellular2

Approaches4

Intercellular2

Translational2

Analysis7

Systems11

Exposure2

Epithelial cells1

Microscopy4

Enables1

Tumor1

Intracellular6

Stem Cell2

Viability1

Mammalian cells2

Human induced pluri1

Concentration6

Experimental1

Proteins3

Researchers2

Stromal2

Reproducible2

Studies1

Grown2

Biologically-relevant1

Microbial1