• Alkaline Phosphatase (AP) and other nonspecific phosphatases are some of Nature's most impressive catalysts, achieving preferential transition state over ground state stabilization of more than 10²²-fold while utilizing interactions with only the five atoms attached to the transferred phosphorus. (rcsb.org)
  • The EC 50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1-10 µg/ml. (abcam.com)
  • Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (geneticsmr.com)
  • Development of a nanobody-alkaline phosphatase fusion protein and its application in a highly sensitive direct competitive fluorescence enzyme immunoassay for detection of ochratoxin A in cereal. (cdc.gov)
  • A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for ochratoxin A (OTA) based on a nanobody (Nb)-alkaline phosphatase (AP) fusion protein was developed. (cdc.gov)
  • Actin induced a 2.9-fold increase in alkaline phosphatase activity in E. coli CC118 with a TnphoA insertion in the hlyB determinant of the recombinant haemolysin piasmid pWAM04. (microbiologyresearch.org)
  • We used an alkaline phosphatase-based reaction to control phosphate release, thereby mimicking biological processes, to monitor calcium phosphate mineralization. (yildiz.edu.tr)
  • Analogous Pi binding measurements with a protein tyrosine phosphatase suggest the generality of this ground state destabilization mechanism. (rcsb.org)
  • Escherichia coli strain 6-171 aervf-supercont1.1.C9, caboodle mainly genome shotgun structure 167,065 bp linear DNA Accession: LENF01000009.1 GI: 1228574971 BioProject BioSample Protein Taxonomy GenBank FASTA Graphics order prandin (repaglinide) 0,5 mg, 1 mg, 2 mg online no prescription Select pay 1228574377 18. (upb.ro)
  • This domain can be found in dual specificity phosphatases. (embl.de)
  • The Cdc25 family of protein dual specificity phosphatases (EC 3.1.3.48 ). (expasy.org)
  • The authors have previously reported that the Salmonella typhimurium SigD protein, a phosphatidylinositol phosphatase involved in invasion of the host cell, inhibits yeast growth, presumably by depleting an essential pool of phosphatidylinositol 4,5- bis phosphate, and also that a catalytically inactive version, SigD R468A , was able to arrest growth by a different mechanism that involved disruption of the actin cytoskeleton. (microbiologyresearch.org)
  • It is concluded that the Salmonella SigD protein deprived of its phosphatase activity is able to disrupt yeast morphogenesis by interfering with Cdc42 function, opening the possibility that the SigD N-terminal region might directly modulate small GTPases from the host during infection. (microbiologyresearch.org)
  • The CRAL-TRIO domain is found in GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs) and a family of hydrophobic ligand binding proteins, including the yeast SEC14 protein and mammalian retinaldehyde- and alpha-tocopherol-binding proteins. (embl.de)
  • Pyridoxal phosphate biosynthetic protein PdxA [Interproscan]. (ntu.edu.sg)
  • Vitamin B6-phosphate phosphatase. (ebi.ac.uk)
  • Vitamin B(6)-phosphate phosphatase. (ebi.ac.uk)
  • To find proteins with nucleotidase activity in Escherichia coli, purified unknown proteins were screened for the presence of phosphatase activity using the general phosphatase substrate p-nitrophenyl phosphate. (rhea-db.org)
  • The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)
  • Herein, we report an engineered fusion protein that not only targets calcium phosphate minerals but also allows monitoring of biomineralization. (yildiz.edu.tr)
  • Acylation of sn glycerol 3 phosphate in Escherichia coli. (illinois.edu)
  • Native and recombinant protein harbored intrinsic magnesium-dependent bisphosphate nucleotidase activity (BPntase), which removed the 3'-phosphate from 3'-5' bisphosphate nucleosides and 3'-phosphoadenosine 5'-phosphosulfate with Km and Vmax values of 0.5 microM and 40 micromol/min/mg. (rhea-db.org)
  • In molecular biology, the DHH phosphatase family is a family of putative phosphoesterases. (wikipedia.org)
  • Rhodanese-like proteins are either composed of a single catlytic rhodanese domain, as found in glpE, or composed of two rhodanese domains, with the C-terminal domain displaying the putative catalytic Cys as observed in Rhobov and rhdA. (expasy.org)
  • Comparative genome analysis suggests genes that play a role in the construction or functioning of large cable bacteria cells: the genomes of Ca . Electrothrix gigas encode a novel actin-like protein as well as a species-specific gene cluster encoding four putative pilin proteins and a putative type II secretion platform protein, which are not present in other cable bacteria. (uibk.ac.at)
  • Kinases and phosphatases are drivers of protein phosphorylation and dephosphorylation events. (kinasebiotech.com)
  • Production of kinases and phosphatases as well as their substrates is essential to study the mechanism of protein phosphorylation and the development of drugs targeting kinase/phosphatase signaling networks. (kinasebiotech.com)
  • Our results suggest that a screening approach using protein crystallography is particularly useful to identify universal fragments for the conserved hydrophilic recognition sites found in target families such as SH2 domains, phosphatases, kinases, proteases, and esterases. (rcsb.org)
  • The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. (embl.de)
  • For example, Escherichia coli contains CoQ8 while Saccharomyces cerevisiae has CoQ6 (Okada et al. (ijbiotech.com)
  • To produce the bi-functional fusion protein, nucleotide sequence encoding combinatorially selected hydroxyapatite-binding peptides (HABP) was genetically linked to the 30 end of the open reading frame of green fluorescence protein (GFPuv) and successfully expressed in Escherichia coli. (yildiz.edu.tr)
  • Our results demonstrate a simple and versatile application of inorganic-binding peptides conjugated with bioluminescence proteins as bi-functional bioimaging molecular probes that target mineralization, and which can be employed to a wide range of biomimetic processing and cell-free tissue engineering. (yildiz.edu.tr)
  • Therefore, for each protein, heavy labeled reference peptides (selected from the shotgun LC-MS/MS experiment) were synthetized. (harvard.edu)
  • These screens identified the presence of nucleotidase activity in three uncharacterized E. coli proteins, SurE, YfbR, and YjjG, that belong to different enzyme superfamilies: SurE-like family, HD domain family (YfbR), and haloacid dehalogenase (HAD)-like superfamily (YjjG). (rhea-db.org)
  • Rhodanese domains, either catalytic or inactive (i.e. where the active-site Cys is replaced by another residue), are also found associated with other protein domains such as MAPK-phosphatases or thiL, an Escherichia coli enzyme involved in thiamin and thiouridine biosynthesis. (expasy.org)
  • Conformational dynamics plays an important role in enzyme catalysis, allosteric regulation of protein functions and assembly of macromolecular complexes. (duke.edu)
  • The initial velocity decreased at enzyme concentrations higher than 0.13 mg protein per 2.0 ml of incubation mixture. (who.int)
  • These studies were conducted to determine if actin influences growth and haemolytic activity of E. coli. (microbiologyresearch.org)
  • Growth of E. coli in the presence of actin resulted in culture supernatant haemolytic activity that was 2.4-, 2.7- and 3.3-fold greater than that of E. coli grown in medium containing BSA, non-supplemented medium, or medium containing heat-denatured actin, respectively. (microbiologyresearch.org)
  • These results indicate that extracellular actin enhances haemolysin production by E. coli and may have implications in the pathogenesis of E. coli infections. (microbiologyresearch.org)
  • The Rho family of GTP-binding proteins has been implicated in the regulation of various cellular functions including actin cytoskeleton-dependent morphological change. (embl.de)
  • The novel actin-like protein was also found in a number of other giant bacteria, suggesting there could be a genetic basis for large cell size. (uibk.ac.at)
  • This actin-like protein (denoted big bacteria protein, Bbp) may have a function analogous to other actin proteins in cell structure or intracellular transport. (uibk.ac.at)
  • In analogy to other actin proteins, Bbp could fulfill a structural role in the cell or potentially facilitate intracellular transport. (uibk.ac.at)
  • NCK is a major adaptor protein involved in membrane receptor signalling and modulation of actin cytoskeleton dynamics. (eu.org)
  • The open reading frame of the target gene was cloned into the pET-32a expression vector to obtain recombinant protein in Escherichia coli DE-3 cells for the subsequent production of polyclonal antibody. (geneticsmr.com)
  • Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)
  • The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). (antikoerper-online.de)
  • Production of the catalytic core of human peptidylglycine α -hydroxylating monooxygenase (hPHMcc) in Escherichia coli. (bu.edu)
  • The concentration range of the 41 proteins covered more than four orders of magnitudes ranging from around 92,000 (Mdh, on acetate medium) to only 2 (YbhA, 3 d into stationary phase) copies per cell. (harvard.edu)
  • The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity. (nih.gov)
  • This coenzyme is essential in energy generation and some other processes including formation of disulfide bonds in proteins, regulation of gene expression and detoxification of reactive oxygen species (ROS) (Groneberg et al. (ijbiotech.com)
  • Our lab focuses on investigating the structure, function, and regulation of proteins involved in cell signaling, epigenetics, and gene regulation. (bu.edu)
  • However, were more likely to have neurologic study suggests E. coli O26 exists as a non-O157 VTEC serotypes have been complications and diabetes mellitus highly dynamic group of organisms increasingly found in the development and require admission to the intensive that can undergo verotoxin gene loss of HUS ( 2 - 4 ). (cdc.gov)
  • pps1Delta mutant strains did not differ from PPS1 strains under any of the conditions tested, but overexpression of the PPS1 protein in S. cerevisiae led to synchronous growth arrest and to aberrant DNA synthesis. (embl.de)
  • Cytotoxic necrotizing factor production by hemolytic strains of Escherichia coli causing extraintestinal infections. (microbiologyresearch.org)
  • A lycopene production pathway including geranylgeranyl diphosphate synthase (crtE), phytoene synthase (crtB), and phytoene desaturase (crtI) from Erwinia herbicola was constructed in two CoQ10-producing E. coli strains. (ijbiotech.com)
  • To confirm that the improvement of CoQ10 production in E. coli Br-lyc was due to lycopene biosynthesis and not just geranylgeranyl diphosphate formation in the lycopene pathway, crtE was only introduced into E. coli Ba and E. coli Br strains. (ijbiotech.com)
  • The Nb28-AP construct was transformed into Escherichia coli BL21(DE3)plysS, and soluble expression in bacteria was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot. (cdc.gov)
  • Search proteins in UniProtKB for this molecule. (rhea-db.org)
  • protein_coding" "AAC73288","bamA","Escherichia coli","BamABCDE complex OM biogenesis outer membrane pore-forming assembly factor [Ensembl]. (ntu.edu.sg)
  • Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
  • The Lipid A 1-Phosphatase, LpxE, Functionally Connects Multiple Layers of Bacterial Envelope Biogenesis. (duke.edu)
  • During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kDa protein Ffh and a 4.5S RNA. (nature.com)
  • Molecular features of phosphatase and tensin homolog (PTEN) regulation by C-terminal phosphorylation. (bu.edu)
  • Escherichia coli O157:H7 str. (up.ac.za)
  • We investigated that E. coli O26 was more virulent the prehospital management of E. than E. coli O157, we performed coli O157 and O26 patients in this an age-matched, nested case-case cohort and found no difference in study of HUS patients and compared pharmacologic intervention or duration host clinical markers, treatment, of delay in admission to hospital. (cdc.gov)
  • A recent such as E. coli O157 ( 1 ). (cdc.gov)
  • Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli. (nih.gov)
  • In contrast to the signal sequences of exported proteins, the bacterial SRP signal sequences are more hydrophobic and are mostly "uncleaved signal sequences" present in membrane proteins that remain in the final protein-chain as transmembrane anchor sequences. (nature.com)
  • The family includes Drosophila prune protein and bacterial RecJ exonuclease. (wikipedia.org)
  • Some bacterial proteins closely related to rhodanese are also thought to express a sulfotransferase activity. (expasy.org)
  • RhdA is a periplasmic protein probably involved in the transport of sulfur compounds. (expasy.org)
  • PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. (antikoerper-online.de)
  • In Vivo and in Vitro Synthesis of Phosphatidylglycerol by an Escherichia coli Cardiolipin Synthase. (duke.edu)
  • Therefore, and is most frequently precipitated of cases, i.e., bloody diarrhea and serodiagnosis of VTEC is considered by infection with verotoxin- abdominal pain, statistical analysis a necessary adjunct to bacteriological producing Escherichia coli (VTEC) showed that children with O26-HUS confi rmation of infection ( 9 ). (cdc.gov)
  • The MKP1/PAC1 family of MAP-kinase phosphatases (EC 3.1.3.48 / EC 3.1.3.16 ). (expasy.org)
  • The Pyp1/Pyp2 family of MAP-kinase phosphatases (EC 3.1.3.48 ). (expasy.org)
  • Insights into Enzymatic Catalysis from Binding and Hydrolysis of Diadenosine Tetraphosphate by E. coli Adenylate Kinase. (umu.se)
  • Creative BioMart offers numerous options for custom protein production services to accelerate kinase/phosphatase biology research and pharmaceutical development. (kinasebiotech.com)
  • The Src Homology 2 (SH2) domain is a major protein interaction module that is central to tyrosine kinase signaling. (eu.org)
  • Effects of Escherichia coli hemolysin in human monocytes. (microbiologyresearch.org)
  • Effect of Escherichia coli β- hemolysin on human peripheral leukocyte viability in vitro. (microbiologyresearch.org)
  • The mission of our lab is to understand the basic biological mechanisms of proteins that are essential to human health and disease. (bu.edu)
  • Mutations identified in many SH2 domain-containing proteins as well as the SH2 domain itself are associated with human diseases ranging from cancers, diabetes, to immunodeficiencies. (eu.org)
  • Human and murine cDNA clones encoded proteins sharing 92% identity and were highly expressed in kidney. (rhea-db.org)
  • Here, we describe a method to detect microcystins in human plasma by using immunocapture followed by a protein phosphatase inhibition assay. (cdc.gov)
  • E. coli Ba and E. coli Br containing dds orthologs encoding for decaprenyl diphosphate synthase (Dds), respectively from Agrobacterium tumefaciens and Rhodobacter sphaeroides were transformed by the lycopene pathway resulting in E. coli Ba-lyc and E. coli Br-lyc. (ijbiotech.com)
  • We analyzed the AST reported for Escherichia coli (blood and urine) and Staphylococcus aureus (blood and lower respiratory tract [LRT]) isolates from April 2020 to March 2021, used AST reporting patterns to assign SR/CR reporting status for hospitals, and compared their CAs. (cdc.gov)
  • At least 35% and 41% of the hospitals had AST data that were suppressed in more than 20% blood isolates for E. coli and S. aureus isolates, respectively. (cdc.gov)
  • Data collection has been were detected in isolates from 2 of Escherichia coli described elsewhere ( 5 ). (cdc.gov)
  • PI transfer protein: the specific recognition of phospholipids and its functions. (embl.de)
  • Phosphatidylglycerol (PG) makes up 5-20% of the phospholipids of Escherichia coli and is essential for growth in wild-type cells. (duke.edu)
  • Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p) and in RhoGAPs, RhoGEFs and the RasGAP, neurofibromin (NF1). (embl.de)
  • Phosphatidylinositol transfer proteins (PITPs) can bind specifically and transfer a single phosphatidylinositol (PI) molecule between phospholipid membranes in an ATP-independent manner in vitro. (embl.de)
  • Second, the cellular concentrations of 41 proteins covering all components of the glycolysis pathway were determined across all samples by SRM and SID [see comments section]. (harvard.edu)
  • With this information, the protein concentrations determined by SRM and/or SID could be transformed to protein copies/cell and a quantitative model was built to translate MS intensities of all quantified proteins to cellular abundance estimates using the Intensity Based Absolute Quantification (iBAQ) approach [ref 4]. (harvard.edu)
  • The domain is named after cellular retinaldehyde-binding protein (CRALBP) and TRIO guanine exchange factor. (embl.de)
  • We use chemical, semi-synthetic, biophysical, and cellular approaches to elucidate mechanisms for how post-translational modifications regulate protein function. (bu.edu)
  • Protein phosphorylation is an extremely important regulatory mechanism in various cellular processes. (kinasebiotech.com)
  • Discovery of a structurally conserved metal-dependent lithium-inhibited phosphomonoesterase protein family has identified several potential cellular targets of lithium as used to treat manic depression. (rhea-db.org)
  • As an essential part of living organisms, proteins play an important role in virtually every process within cells, such as cell signaling, cell cycle, cell adhesion, cell metabolism, and immune responses. (kinasebiotech.com)
  • These proteins' concentrations were determined in each sample using stable isotope dilution (SID) and selected reaction monitoring (SRM) LC-MS/MS [Liquid chromatography-mass spectrometry] analysis [refs 23, 24] (Supplementary Tables 2 and 3). (harvard.edu)
  • Schematic representation of hTLK1B-pETDUET-1-Lambda Protein Phosphatase expression vector (A) and coexpression of recombinant 6×His-tagged hTLK1B along with bacteriophage lambda protein phosphatase at 25 °C at different IPTG concentrations. (kinasebiotech.com)
  • We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)
  • This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
  • Silk-elastin-like polymers (SELPs) are PBPs composed of multiple repeats of both silk- and elastin-like motifs that combine the outstanding mechanical and biological properties of both these proteins. (springeropen.com)
  • Many proteins have become very attractive sources for therapeutic development due to their high intrinsic potency and selectivity for biological targets. (kinasebiotech.com)
  • CATH: Protein Structure Classification Database by I. Sillitoe, N. Dawson, T. Lewis, D. Lee, J. Lees, C. Orengo is licensed under a Creative Commons Attribution 4.0 International License . (cathdb.info)
  • Iron-sulfur protein maturation in Helicobacter pylori:Identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets. (uga.edu)
  • The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. (embl.de)
  • Our current focus is on understanding the function and regulation of deubiquitinases and RNA-modifying proteins, which are emerging as attractive therapeutic targets in several cancers. (bu.edu)
  • They are able to bind specific motifs containing a phosphorylated tyrosine residue, propagating the signal downstream by promoting protein-protein interactions and/or modifying enzymatic activities. (eu.org)
  • Fe-S clusters are present in more than 300 different types of enzymes or proteins and play crucial roles in fundamental life processes such as respiration, photosynthesis and nitrogen fixation. (uga.edu)
  • Specifically, [they] selected the enzymes and iso-enzymes of the glycolytic pathway (including proteins with hypothetical function), tricarboxylic acid cycle enzymes and a few other proteins (Supplementary Table 1). (harvard.edu)
  • Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
  • In this study, the effect of concomitant lycopene biosynthesis on CoQ10 accumulation in transformed Escherichia coli DH5α was studied. (ijbiotech.com)
  • Tyrosine specific protein phosphatases (PTPases) contain two conserved cysteines, the second one has been shown to be absolutely required for activity. (embl.de)
  • Its activity is directed by intracellular signals mediated by various types of receptors such as G protein-coupled receptors. (embl.de)
  • This assay was compared with LC-MS/MS, and the results indicated the reliability of Nb-AP fusion protein-based dc-FEIA for monitoring OTA contamination in cereal. (cdc.gov)
  • The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)
  • Analysis of the predicted amino acid sequence suggests that the protein consists of an active phosphatase domain, an inactive phosphatase-like domain, and an NH2-terminal extension. (embl.de)
  • An amino-terminal portion conserved among a subset of Dbl family proteins is sufficient for the binding of Gbetagamma. (embl.de)
  • Nature has refined structural proteins to perform defined functions through the association of specific amino acid sequences in various combinations, creating diverse materials with remarkable properties. (springeropen.com)
  • These have served as a source of inspiration for a new class of bio-engineered materials, the protein-based polymers (PBPs), founded on conservative amino acid motifs found in nature. (springeropen.com)
  • KdpD is a four-spanning membrane protein that has two large cytoplasmic domains at the amino- and at the carboxyterminus, respectively. (nature.com)
  • The information within the KdpD protein that confers SRP interaction was found in the amino-terminal cytoplasmic domain of KdpD, particularly at residues 22-48. (nature.com)
  • The amino-terminal NG domain of SRP is bound to the ribosomal proteins uL23 and uL29, next to the tunnel exit and the carboxy-terminal M domain to the ribosomal 23S RNA 6 . (nature.com)
  • Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling. (embl.de)
  • Here, we show the association of Dbl with G protein betagamma subunits (Gbetagamma) in transient co-expression and cell-free systems. (embl.de)
  • SH2 domains are phosphotyrosine recognition domains, often mediating transient interactions with target proteins. (eu.org)
  • The utility of GFPuv-HABP fusion protein was assessed for both time-wise monitoring of mineralization and the visualization of the mineralized tissues. (yildiz.edu.tr)
  • The increase in mineral amount was observed using the fusion protein at different time points. (yildiz.edu.tr)
  • P.2 left column 2nd paragraph:'Second, [investigators] accurately quantified a subset of identified proteins to establish a 'calibration' for the MS [mass spectrometry] intensities determined for all identified proteins. (harvard.edu)
  • Although SAD experiments are commonly conducted at cryogenic temperatures to mitigate the effects of radiation damage, such temperatures can alter the conformational ensemble of the protein and may impede the merging of data from multiple crystals due to non-uniform freezing. (anl.gov)
  • The Tir protein of EPEC binds NCK1/NCK2 SH2 domains through a high affinity pYDEV motif ( Frese,2006 ). (eu.org)