• cofactor
  • 1. A process for preparing phenylalanine which comprises preparing an aqueous reaction mixture consisting essentially of phenylpyruvic acid or phenylpyruvate, pyridoxal -5-phosphate as a cofactor and an amine donor selected from the group consisting of L-glutamic acid and L-aspartic acid, and contacting said reaction mixture with cells immobilized with a polyazetidine polymer, the cells having transaminase activity to obtain said phenylalanine in high yield. (google.ca)
  • Because the mechanism involves a Fe(IV)=O (as opposed to a peroxypterin) hydroxylating intermediate, oxidation of the BH4 cofactor and hydroxylation of phenylalanine can be decoupled, resulting in unproductive consumption of BH4 and formation of H2O2. (wikipedia.org)
  • activity
  • A process is disclosed for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. (google.ca)
  • The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed. (google.ca)
  • 13. A composition suitable for use in converting phenylpyruvic acid or phenylpyruvate to phenylalanine comprising immobilized cells having transaminase activity, said cells being immobilized with a polyazetidine polymer. (google.ca)