Phenotypic lines cell line. Medical search. Frequent questions

To demonstrate suitability of the panels for high throughput screening, the EGFR panel was selected for evaluation using Corning Epic Technology, a label-free platform that uses optical biosensors for high sensitivity biochemical and cell-based assays. (atcc.org)
What other types of targets has Corning Epic Technology been used for in cell-based assays? (atcc.org)
Epic has been used successfully for a wide range of targets in cell-based assays. (atcc.org)
Nine of these were tested and confirmed in cell assays, documenting the feasibility of using phenotypic information to infer molecular interactions and hinting at new uses of marketed drugs. (embl.de)

Methods: Expression of postulated stem cell markers aldehyde dehydrogenase (ALDH), CD44, and CD133 was analyzed in UM cell lines and primary UM short-term cultures (STCs) established from tumor samples. (whiterose.ac.uk)
Furthermore, there is an absence of a cellular hierarchy in cell lines and all cells in culture are able to drive tumor progression. (whiterose.ac.uk)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
Both EMT and CSC phenotypic changes are associated with tumor recurrence. (techscience.com)

Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
Qualitative and quantitative analyses of the rare phenotypic variants in in vitro culture systems is necessary for the understanding of cell differentiation in cell culture of primary cells or cell lines. (nih.gov)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)

Last, we show that established UM cell lines and UM STCs are plastic in nature and switch their phenotype in response to environmental stimuli. (whiterose.ac.uk)
Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
To document its functions in mammalian cells in more detail, the authors reported previously on the phenotype of cultured cancer cells after Plk1 depletion by the use of interfering RNA (RNAi). (harvard.edu)
Slide-based cytometry combines image acquisition and data treatment, and associates the power of flow cytometry (FCM) and the resolution of the microscopic studies making it suitable for the analysis of cells with rare phenotype. (nih.gov)
Cells with the rare stem-cell like phenotype can be efficiently identified in the undisturbed cultures. (nih.gov)

Do you have other phenotypic characterization data for the ATCC Genetic Alteration Panels in addition to validated gene mutations? (atcc.org)

Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

Similarly, non-small cell lung cancer patients whose tumors show moderate Plk1 expression survive significantly longer than those patients with high levels of Plk1 transcripts. (harvard.edu)
Analysis of patients with head and neck squamous cell carcinomas, oropharyngeal carcinomas, ovarian and endometrial carcinomas, and esophageal and gastric cancer has revealed a close correlation between lower survival rates and higher Plk1 expression levels. (harvard.edu)
This extends also to suspension cells such as primary blood cells, which can be used to evaluate the effects of chemotherapeutic drugs on leukemic cells obtained directly from patients. (atcc.org)
Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
Imatinib approved by the US FDA (Food and Drug Administration) in 2001 was the mainstay of first-line drug for CML patients as the first-generation TKIs. (jcancer.org)

Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)

This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed "cancer stem cells" (CSCs). (whiterose.ac.uk)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)

Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)

Halliwell JA, Gravells P & Bryant HE (2020) DNA fiber assay for the analysis of DNA replication progression in human pluripotent stem cells . (sheffield.ac.uk)

In addition to validating the gene copy number variations and mutations within cell lines, ATCC also tests the related gene expression by real-time PCR and related protein expression by Western blot. (atcc.org)
Rix and his colleagues began the study with a phenotypic screen of 240 compounds against 20 non-small cell lung cancer cell lines harboring a variety of driver mutations. (genomeweb.com)

Analysis of proteomic alteration was carried out using 2D-DIGE on both the total cell lysate and the hydrophobic proteomes. (dcu.ie)
Green shading indicates the GFP coding sequence provided by the donor.Physique S2 Western immunoblot analysis of genome-edited cell lines. (accessibletech4all.org)
Physique S3 Fluorescence microscopy analysis of genome-edited cell lines. (accessibletech4all.org)
Scale bar, 10 m. (b HC-030031 and c) Immunostaining analysis of genome edited cell lines. (accessibletech4all.org)
Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
The cells with a given fluorescence intensity can then be located easily on the recorded image of the culture plate for further analysis. (nih.gov)
Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same time, the method associates the advantage of the FCM analysis and the microscopic observation. (nih.gov)
Analysis of XY byg/byg gonads at 11.5 d post coitum reveals a growth deficit and a failure to support mesonephric cell migration, both early cellular processes normally associated with testis development. (plos.org)
And gene set enrichment analysis, immune infiltration, and the proposed cell cycle algorithm were applied to explore the biological functions of different subtypes. (bvsalud.org)
RESULTS: Comprehensive genomic analysis of 1,143 DLBCL samples identify 2 molecularly, prognostically relevant subtypes: immune-enriched (IME) and cell-cycle-enriched (CCE). (bvsalud.org)
Comprehensive multiplatform analysis of Luminal B breast cancer (LBBC) specimens identifies two molecularly distinct, clinically relevant subtypes: Cluster A associated with cell cycle and metabolic signaling and Cluster B with predominant epithelial mesenchymal transition (EMT) and immune response pathways. (bvsalud.org)

Although these cells represent only about 1% of the total population as calculated by flow cytometry, they can be identified in the culture plate with high precision by microscopy. (nih.gov)

However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
Early fate decisions in hematopoietic stem and progenitor cells. (lu.se)

Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)

Moreover, we describe experiments showing the presence of activated MKK4, a direct target of MAP3K4, and activated p38 in the coelomic region of the XY gonad at 11.5 d post coitum, establishing a link between MAPK signalling in proliferating gonadal somatic cells and regulation of Sry expression. (plos.org)

These cell lines include a lung adenocarcinoma (A549), a non small cell lung carcinoma cell line (DLKP), normal bronchial epithelial cell line (NHBE), a breast adenocarcinoma (MCF-7) and human mammary epithelial cells (HMEC). (dcu.ie)

In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
Tissues and Cells. (lu.se)

Nestlé Health Science knows how to develop nutraceutical compounds as first-line treatment for people in need, thanks to their distinguished commitment to cutting-edge science and clinical validation," said Josua Jordi, CEO of EraCal. (gruenden.ch)

We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). (biomedcentral.com)

Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
A new study from the Erikson lab by Xiaoqi Liu and Ming Lei (just appearing in Molecular and Cellular Biology ) offers important insight into the function of Polo-like kinase 1 and its role in cell regulation and carcinogenesis in mammalian cells. (harvard.edu)
In addition, we have characterized the cell growth kinetics, cell morphology, the related protein expression level, and protein cellular location by immunofluorescence staining. (atcc.org)
Targets for drugs have so far been predicted on the basis of molecular or cellular features, for example, by exploiting similarity in chemical structure or in activity across cell lines. (embl.de)

Proteins were identified in the total cell lysates that suggest that DLKP-M is mesenchymal-like in nature as originally described and that the interconversion process observed between the clones is regulated at least by key proteins involved in protein metabolism. (dcu.ie)
Lysates of (a) BSC-1 or (b to d) SK-MEL-2 cells were immunoblotted against CLTA, DNM2, RFP, GFP, or actin. (accessibletech4all.org)

Conclusions: We hypothesize that this capacity to undergo phenotypic plasticity may be a consequence of neural crest lineage and renders the exploration of the CSC hypothesis extremely challenging in UM. (whiterose.ac.uk)

Physique S4 Endocytic protein lifetime distribution of genome-edited cell lines. (accessibletech4all.org)
The Sanger library is the only tool that allows both whole genome CRISPR KO interrogation and sophisticated phenotypic readout in a convenient arrayed format. (sigmaaldrich.com)

Results: We demonstrate that expression of ALDH, CD44, and CD133 does not select for a subpopulation of stem-like cells in either UM cell lines or UM STCs. (whiterose.ac.uk)
BFP expression in A549-Cas9-Blast stable cells infected with arrayed CRISPR lenti-gRNA viruses, seven days post infection, 20X magnification. (sigmaaldrich.com)
When analyzing four closely related hiPSC-CM lines, we show that both AmpliSeq and RNA-seq capture similar global gene expression patterns consistent with known sources of variations. (biomedcentral.com)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

Is it possible to test the effects of drugs like the EGFR inhibitors in primary cells? (atcc.org)
Using unsupervised hierarchical clustering they found that drugs to the same target clustered together in terms of their effects on these cell lines. (genomeweb.com)
This has enabled the group to develop drugs which can specifically kill the tumour cells rather than just all dividing cells (personalized therapy). (sheffield.ac.uk)
We used phenotypic side-effect similarities to infer whether two drugs share a target. (embl.de)

Genetic and biochemical experiments with several different organisms have documented that the polo-like kinase (Plk) family serves as a vital regulator in many aspects of cell cycle progression. (harvard.edu)

The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
In order to facilitate basic and translational cancer research, ATCC has designed and validated a number of Genetic Alteration Cell Panels targeting the key molecular pathways identified in these studies. (atcc.org)
Dr. Fang Tian, Lead Scientist, Director of Biological Content for ATCC, has extensive experience in cell biology and molecular biology. (atcc.org)

Fixed (b) BSC-1 mkCLTAEN and (c) SK-MEL-2 hCLTAEN-all cell lines were stained for clathrin heavy chain (CHC) and visualized by epi-fluorescence and TIRF microscopy, respectively. (accessibletech4all.org)
The software allows the automated segmentation of the nuclei in a very dense cell environment, the measurement of the fluorescence intensity of each nucleus and the recording of their position in the plate. (nih.gov)

Finally, the phenomenon of phenotypic plasticity in response to environmental factors was explored. (whiterose.ac.uk)

Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)

EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)

Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)

Adherence was altered in one of the three cancer cell lines to the extracellular matrix proteins collagen type IV and fibronectin. (dcu.ie)
Lifetime distribution for endocytic proteins was determined by the elapsed time between the appearance and disappearance of fluorescent structures present in the time series (6 min) from TIRF microscopy time-lapse videos of (a) BSC-1, (b) SK-MEL-2 CLTA-RFP and (c) SK-MEL-2 DNM2-GFP cell lines. (accessibletech4all.org)
In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
By understanding the control of the fidelity of DNA replication in normal cells we aim to understand why defects in the proteins involved are associated with cancer. (sheffield.ac.uk)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
The cytoskeleton is also important for certain processes inside the cells, such as the movement of proteins within the cell and the breakdown of unneeded proteins. (medlineplus.gov)
Filamin A can also bind to many other proteins in the cell to carry out various functions, including the attachment of cells to one another (cell adhesion), cell movement (migration), determination of cell shape, the relay of signals within cells, and cell survival. (medlineplus.gov)
Filamin A is also involved in the organization of the extracellular matrix, which is the lattice of proteins and other molecules outside the cell. (medlineplus.gov)
Filamin A binds to proteins called integrins, which span the cell membrane and anchor cells to the extracellular matrix. (medlineplus.gov)

She oversees human, animal cell lines and hybridomas, and product development in the Cell Biology General Collection at ATCC. (atcc.org)
In this paper we develop a method that applies these principles to a particularly hot problem in cell biology, the study of stem cell like cells in cultures of primary cells, cancer cells, and various cell lines. (nih.gov)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)

Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
This approach revealed an essential role of Plk1 for cell proliferation and viability. (harvard.edu)
Because inhibition of cell proliferation and activation of apoptosis are fundamental approaches to cancer therapy, it was of interest to test the effect of Plk1 depletion in normal human cells. (harvard.edu)
no apparent proliferation defect or cell cycle arrest was observed after Plk1 depletion. (harvard.edu)

F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)

Through this binding, cells are correctly positioned and signals can be exchanged between the cell and the extracellular matrix. (medlineplus.gov)

The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)

Taken together, these data suggest new approaches for the study of Plk1 function in cell regulation and survival, and also emphasize the potential of Plk1 as a target in cancer therapy. (harvard.edu)

Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)

DLKP is a heterogeneous cell line composed of at least three subpopulations. (dcu.ie)
Data also shows the presence of protein in the heterogeneous population that are not present or are over expressed in the clonal populations indicating cell-cell communication. (dcu.ie)

EraCal's custom-built platform overcomes these challenges by combining state-of-the-art microscopy, with the power of large-scale phenotypic drug discovery. (gruenden.ch)

Using both siRNA knockdown and small molecule-based targeting, Rix and his colleagues looked at how inhibition of these kinases affected cell viability, finding that targeting them singly had little effect. (genomeweb.com)
Simultaneous inhibition of these targets, however, significantly decreases cell viability, indicating that ceritinib's polypharmacological activity is key to its effectiveness in non-ALK-rearranged cell lines. (genomeweb.com)

Like the other ALK inhibitors tested, ceritinib was effective against the ALK-rearranged cell line used in the study, but it was also effective in several additional cell lines where other ALK inhibitors were not. (genomeweb.com)

In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)

Moreover, Plk1 depletion in cancer cells over an extended time induced apoptosis (cell death), as supported by the appearance of sub-genomic DNA, the activation of caspase 3, and the formation of fragmented nuclei. (harvard.edu)
However, upon subsequent severe depletion of Plk1, p53-depleted MCF10A cells were arrested prior to mitosis, but subsequently exhibited sub-genomic DNA, consistent with apoptosis events. (harvard.edu)

The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)

Moreover, it easily enters cells and binds to toll-like receptor 4. (nature.com)

their evolution from distinct cell lineages. (lu.se)

In addition to its intended target ALK and the known off-target IGF1R, the drug bound to kinases including FAK1, RSK1/2, ERK1/2, CAMKK2, and FER, with FAK1 and RSK1/2 being the most highly enriched in the cell lines where ceritinib was effective. (genomeweb.com)
This led the researchers to test a combined ceritinib-paclitaxel treatment, which they found to be particularly effective, including in NSCLC lines where single-drug treatment was not effective. (genomeweb.com)
To fight Obesity, EraCal has been developing a novel anti-obesity drug candidate, Era-379, based on its phenotypic drug discovery technology platform. (gruenden.ch)

Do you have purified genomic DNA for the cell lines represented in the ATCC Genetic Alteration Panels? (atcc.org)
ATCC is currently producing genomic DNA for most of the cell lines represented in the 10 Genetic Alteration Panels. (atcc.org)

There are now a number of publications demonstrating successful application of Epic for G proteincoupled receptors (GPCRs), where DMR profiles across many different cell backgrounds are highly characteristic of the downstream signaling pathway. (atcc.org)

The images of cell populations were collected by a two-photon microscope and processed by a software developed by us. (nih.gov)

Phenotypes were characterised and 5-FU was found to induce and reduce invasion in various cell lines. (dcu.ie)

In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)

Top panel: The number of genotyped clones is usually given for each target in each cell type, along with the number (percentage) of correctly tagged clones having at least one (but less than all) allele tagged, or all alleles tagged. (accessibletech4all.org)

Note that in all cases, cells were enriched by FACS for the cognate fluorescent marker prior to limiting dilution and genotyping. (accessibletech4all.org)

Anatomy35

Organisms12

Diseases34

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes67

Disciplines and Occupations1

Information Science3

Assays4

Tumor5

Differentiation22

Endothelial cells1

Phenotype5

Characterization1

Vitro3

Patients5

Genes1

Cancer stem4

Embryonic stem1

Pluripotent stem1

Mutations2

Analysis11

Flow Cytometry1

Progenitor cells4

Physiological1

Somatic cells1

Epithelial cells1

Tissues3

Compounds1

Obtained from human in1

Cellular4

Lysates2

Neural crest1

Genome2

Expression5

Drugs4

Biochemical1

Molecular3

Fluorescence2

Plasticity1

Lineage1

Progression2

Functional1

Proteins9

Biology2

Fetal bovin1

Characteristics1

Proliferation4

Fibroblast3

Extracellular matrix1

Regenerative Medicine1

Survival1

Sensitivity1

Heterogeneous2

Microscopy1

Inhibition2

Inhibitors1

Differentiate1

Apoptosis2

Methods2

Binds1

Lineages1

Drug3

Genomic2

Downstream1

Populations1

Invasion1

Enrich1

Percentage1

FACS1