Phenotype cdna expressed sequence tags. Medical search. Frequent questions

Entrez is NCBI's primary text search and retrieval system that integrates the PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, structure, gene, genome, genetic variation and gene expression. (nih.gov)
The BioProject database is a searcheable collection of complete and incomplete (in-progress) large-scale molecular projects including genome sequencing and assembly, transcriptome, metagenomic, annotation, expression and mapping projects. (nih.gov)
Genome and exome sequencing were performed for five unrelated cases of IRD with no identified variant. (nature.com)
3 When no pathogenic variant is identified, exome sequencing or genome sequencing may help uncover the variants missed by panel-based screening. (nature.com)
As the common IRD variants have largely been identified, exome sequencing or genome sequencing identify rare variants that require functional validation. (nature.com)
Using exome sequencing and genome sequencing, we document four novel DYNC2H1 pathogenic variants, and one variant previously reported in five unrelated cases of nonsyndromic progressive IRD. (nature.com)
Additional MPSS signatures not matching to known grape ESTs can extend our knowledge of the V. vinifera transcriptome, particularly when these data are used to assist in annotation of whole genome sequences from Vitis vinifera . (biomedcentral.com)
There are works about, how single nucleotide polymorphisms, or SNPs (pronounced «snips»), in the human genome correlate with disease, with drug response, and other phenotypes. (andrologica.org)
First, Tim Graubert and colleagues describe the whole-genome sequencing of an MDS-derived secondary AML tumor and a matched normal (skin) sample. (massgenomics.org)
Governments in the industrialized countries have handed over the human genome to private ownership together with the most triumphant hyperboles to boot, notwithstanding that it was mapped and sequenced at great public expense. (biosafety-info.net)
The Human Genome Project, (HGP) an international public consortium of research laboratories led by the United States, and Celera, a private American company, made the announcement jointly, ending months of competition to complete the first sequence of the human genome. (biosafety-info.net)
The human genome has been sequenced separately and independently with major public finance, from the United States and the European Community. (biosafety-info.net)
Celera kept its own human genome data secret while benefiting from free access to the public database throughout the period that the company was busy sequencing, thereby significantly reducing the time and effort needed to complete the task. (biosafety-info.net)
This thematic series of companion papers constitutes the initial exploration of the genome biology of the waterflea (Daphnia) building on the first complete crustacean genome sequence. (biomedcentral.com)
Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)

Gene expression in hESC has been investigated by a variety of techniques including massively parallel signature sequencing (MPSS), serial analysis of gene expression (SAGE), expressed sequence tag (EST) scan, large scale microarrays, focused cDNA microarrays, and immunocytochemistry [5-7]. (health-ground.com)
RNA Isolation and cDNA Microarrays. (lu.se)
Microarrays were made from expressed sequence tags that were isolated from cDNA libraries of porcine ovary. (appliedbioinfo.com)

Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
Among the matched sequences, ESTs were identified from tissues other than berries or from berries at different developmental stages. (biomedcentral.com)
SNPs can be isolated from cDNA, messenger RNA, and ESTs. (andrologica.org)

Here we performed mRNA profiling using Massively Parallel Signature Sequencing (MPSS) and combined it with available Expressed Sequence Tag (EST) data. (biomedcentral.com)
Positions Contents --------- -------- 01-05 'LOCUS' 06-12 spaces 13-28 Locus name 29-29 space 30-40 Length of sequence, right-justified 41-41 space 42-43 'bp' 44-47 spaces 48-54 DNA, RNA, mRNA, rRNA, tRNA or cRNA, left justified 55-55 space 56-63 'linear' followed by two spaces, or 'circular' 64-64 space 65-67 The division code (see '3.1. (nig.ac.jp)
In breast carcinoma specimens, lymph node status was significantly associated with HIF-1α TAG mRNA levels ( P = 0.037). (biomedcentral.com)
Significant statistical association was found between tumour grade and HIF-1α TAG ( P = 0.048), and total HIF-1α ( P = 0.048) mRNA levels. (biomedcentral.com)
HIF-1α TAG mRNA levels were also inversely correlated with both oestrogen and progesterone receptor status ( P = 0.005 and P = 0.033 respectively). (biomedcentral.com)
Univariate analysis showed that high HIF-1α TAG mRNA levels correlated with shortened metastasis free survival ( P = 0.01). (biomedcentral.com)
Our results show for the first time that mRNA expression of a HIF-1α TAG splice variant reflects a stage of breast cancer progression and is associated with a worse prognosis. (biomedcentral.com)

ClinVar is a public archive of submitted reports of clinically relevant human genetic variants and their relationships to phenotypes, with supporting evidence. (nih.gov)
ClinVar provides standardized nomenclature for variants and phenotypes, a review status for variants, and links to related NCBI literature and molecular databases. (nih.gov)
All identified variants affect either a retina-predominant transcript or are hypomorphic, suggesting a basis for a nonsyndromic retinal phenotype. (nature.com)
Next-generation sequencing identified two de novo truncating variants in the Src-family tyrosine kinase, LYN , p.Y508*, p.Q507* and a de novo missense variant, p.Y508F, that result in constitutive activation of Lyn kinase. (nature.com)
Unfortunately, none of the transcripts that match with at least one of the elongated tags that lie in close proximity to the supergene was found in the list of differentially expressed transcripts with phenotype-consistent variants. (peerj.com)

These tag-based technologies, which do not require a priori knowledge of genomic sequence, are well-suited for transcriptional profiling. (biomedcentral.com)

1111, Yata, Mishima, Shizuoka 411-8540, Japan REFERENCE 2 AUTHORS Mishima,H., Shizuoka,T. and Fuji,I. TITLE Glyceraldehyde-3-phosphate dehydrogenase expressed in human liver JOURNAL Unpublished (2002) COMMENT Human cDNA sequencing project. (nig.ac.jp)

pqbp1 and wbp11 genes are similarly expressed in mesoderm and neural tissues during Xenopus development. (xenbase.org)
To examine the possibility of VEN-1 being a secreted protein, we tested whether forced expression of the ven-1 transcript under the control of heterologous promoters could rescue the Ven phenotype in tissues. (wormbook.org)
Its forced expression in neuronal and intestinal tissues also resulted in partial rescue of the phenotype. (wormbook.org)
HIF-1α isoforms containing a three base pairs TAG insertion between exon 1 and exon 2 (designated HIF-1α TAG ) and HIF-1α 736 mRNAs were found expressed at higher levels in oestrogen receptor (OR)-negative carcinomas compared to normal/benign tissues ( P = 0.009 and P = 0.004 respectively). (biomedcentral.com)
Based on oil accumulation dynamics of V. galamensis seeds, we harvested seed samples from three stages (17, 38, and 45 days after pollination, DAP) representing the initial, fast and final EFA accumulation phases, and one mixed sample from different tissues for RNA-sequencing, with three biological replicates at each sample. (researchsquare.com)

The sequence depth of MPSS allowed us to capture and quantify almost all the transcripts at a specific stage in the development of the grape berry. (biomedcentral.com)
The number and relative abundance of transcripts from stage II grape berries was defined using Massively Parallel Signature Sequencing (MPSS). (biomedcentral.com)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)
An expressed sequence marker or EST (acronym in English: Expressed Sequence Tag) is a small subsequence of a transcribed nucleotide sequence (encoding a protein or not). (andrologica.org)
We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)

More than 50 million high-quality reads from a cDNA library were generated and de novo assembled. (biomedcentral.com)

We used the in vitro / in vivo dual expression system plasmid, pIVEX, to express Danio rerio pol β ( Danio pol β) for biochemical characterization. (biomedcentral.com)
Danio pol β encoded by the in vitro / in vivo -compatible pIVEX plasmid was expressed in E. coli BL21(DE3), BL21(DE3)pLysS, and KRX, and in vitro as a C-terminal His-tagged protein. (biomedcentral.com)

The authors undertook systematic sequencing of U2AF1 exons in 150 MDS cases, and found that 8.7% had mutations at Ser34. (massgenomics.org)
A) Representative western blot analysis of cell lysate from stable HEK293 cells expressing WT and traffic deficient LQT2 mutations in the HERG 1a subunit grown at 37°C and 27°C. (B) Representative western blot analysis of cell lysates from stable HEK293 cells expressing WT and traffic deficient LQT2 mutations in the HERG 1a subunit grown in the presence (+) or absence (−) of 10 µM E4031 for 36 hours. (bioz.com)

In contrast, the gene sequences and gene maps produced by the public consortium have been deposited regularly within 24 hours of completion in GenBank, a public database set up in the early 1980s when DNA sequencing began, access to which is totally free. (biosafety-info.net)

The ven-1 transcript encoded a polypeptide of 86 amino acid residues, including the signal sequence at the N-terminus, suggesting that VEN-1 is a secreted protein (Figure 1B). (wormbook.org)
The forced expression of the transcript in body wall muscle tissue rescued the Ven phenotype as efficiently as the genomic fragment did (Figure 2C). (wormbook.org)
We also examined the temporal requirement of ven-1 activity by expressing the transcript using a heat shock promoter. (wormbook.org)
However, the recovery ratio gradually decreased after the comma stage, and expression of the transcript after the 1.5-fold stage failed to rescue the Ven phenotype. (wormbook.org)

The M. persicae cDNA libraries include ones made from sexual and asexual whole aphids, guts, heads, and salivary glands. (nih.gov)
In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. (nih.gov)
From cDNA libraries, we isolated a 345 bp cDNA clone corresponding to the C53A.2 gene assigned on WormBase. (wormbook.org)

The ven-1 cDNA, composed of three exons, is shown at the bottom. (wormbook.org)

Conserved Domains is a database of protein domains represented by sequence alignments and profiles for protein domains conserved in molecular evolution. (nih.gov)
A) Western blot of membrane protein extracts from stable HEK293 cell lines expressing full-length WT or LQT2 mutant forms of HERG 1a channel subunit. (bioz.com)
B) Western blots of membrane protein extracts from stable HEK293 cell lines expressing full-length WT or LQT2 mutant forms of HERG 1a channel subunit before (−) and after treatment (+) with N-glycosidase F. Western blots were probed with anti-C terminal HERG antibody and were repeated twice (n = 2). (bioz.com)
DEAF-1 is maternally expressed, and the encoded protein is broadly distributed throughout the early embryo. (sdbonline.org)
in which the 472 bp upstream region of the ven-1 open reading frame was fused to green fluorescent protein (GFP) cDNA (Figure 2A). (wormbook.org)
The cDNA of Danio rerio pol β encodes a protein of 337 amino acids, which is 2-3 amino acids longer than other pol β proteins, and contains a P63D amino acid substitution, unlike mammalian pol βs. (biomedcentral.com)

In the Xenopus embryo , XProfilin2 is temporally expressed throughout embryogenesis and it is spatially expressed in cells undergoing morphogenetic movement during gastrulation. (xenbase.org)
G) Translation of C-terminal myc -tagged Xenopus PQBP1 (PQ- myc ) was blocked by co-injection of pqbp1 MO1 but not control MO (CT). (xenbase.org)

Deep cDNA sequencing demonstrated that both wild-type and mutant alleles were expressed . (massgenomics.org)

Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

11 No mutant mice retina phenotype has been documented. (nature.com)
A) Western blot analysis of cell lysates from transiently transfected HEK293 cells co-expressing WT HERG 1a cmyc tagged subunit alone, WT HERG 1b untagged alone, HERG 1a cmyc with HERG 1b or LQT2 mutant HERG 1a cmyc with HERG 1b. (bioz.com)
double mutant had a stronger phenotype than each single mutant, with its penetrance of the Ven phenotype being higher than that of the ven-1 mutant and its growth being much slower than that of the unc-54 mutant. (wormbook.org)

Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

E) Injection of XProfilin2 MO inhibits gastrulation and results in a similar gastrulation-defect phenotype as overexpression of XProfilin2 RNA (see A). This phenotype is reversed by injection of δN- XProfilin2 but not δN- XProfilin1 (F). Quantitation of phenotypic results of XProfilin2 -depletion studies. (xenbase.org)

Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties in vitro . (aacrjournals.org)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

Viruses with H5 or H7 subtypes occasionally acquire a multibasic cleavage site in their HA ( 8 ), which results in a highly pathogenic phenotype. (cdc.gov)

H) Phenotypes of tailbud stage embryos dorsally targeted with control (CT) or the indicated amount of pqbp1 MO1. (xenbase.org)

The searching for matches between the sequenced transcripts and the elongated tags that lie in close proximity to the supergene is also appropriate. (peerj.com)

RNA interference experiments using this cDNA sequence reproduced the Ven phenotype. (wormbook.org)

Previous studies have reported that a mutation in the gene encoding myosin-4 ( unc-54 ) suppresses the Mua phenotype of mua-1 mutants (Plenefisch et al. (wormbook.org)
The resulting blockade of RNA splicing or translation allows loss-of-function phenotypes to be determined within days, contrasting the months that can be required to obtain homozygous mutants. (cdc.gov)

Thus, vertebrate HB9/MNR2 genes are expressed specifically in somatic motoneurons and are essential for distinguishing motoneuron/interneuron cell types. (jneurosci.org)
Both genes are expressed in the nascent mesoderm and neurectoderm , and morpholino knockdown of either causes defects in differentiation and morphogenesis of the mesoderm and neural plate . (xenbase.org)

In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (nih.gov)
The most striking difference between JAK3 and the other JAKs was the presence of 2 stretches of additional amino acid sequences of 147 and 28 residues which spanned between amino acid positions 322 to 469 and 632 to 660, respectively. (appliedbioinfo.com)

of Two Human Thyroid cDNAs Encoding New Members of the NADPH Oxidase Family.pdf "Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family" (PDF). (wikipedia.org)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
A DNA sequence encoding the Human CD44/LHR/MDU2/MDU3/MIC4 Gln21-Pro220 is expressed with a Fc tag at the C-terminus. (novoprolabs.com)
450 /chromosome="12" /clone="GT200015" /clone_lib="lambda gt11 human liver cDNA (GeneTech. (nig.ac.jp)
2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . (en-academic.com)

Using Illumina platform, we have generated a total of 265 million raw cDNA reads. (researchsquare.com)

The best-aligned sequences were annotated with gene ontology groups. (frontiersin.org)

16S rDNA sequencing and metabolomic analyses showed that probiotics effectively reduced the abundance of harmful gut microbiota, such as Firmicutes (p = 0.005), while concomitantly increasing the abundance of beneficial gut microbiota in NAFLD mice, such as Actinobacteriota (p = 0.378), to improve NAFLD. (bvsalud.org)

EU COST Next generation sequencing data analysis network and training consortium. (mpg.de)

These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
cDNA microarray data (7). (lu.se)
Notice for patent related sequence data 4. (nig.ac.jp)

Gene expression was measured by hybridization of fluorescently labeled cDNA (preovulatory estrogenic or -luteinized) to the microarray. (appliedbioinfo.com)
Reductions in SRC64B expression levels suppress a Wnt5 / derailed -dependent dominant gain-of-function phenotype,and increased levels of either SRC64B or SRC42A enhance Wnt5 / derailed -mediated axon commissure switching. (biologists.com)
In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
Compared to those of the DUSP3-expressing cells, we found the expression and distribution of ZO-1, a TJ-anchoring molecule, were abnormal in DUSP3-deficient cells. (biomedcentral.com)
Its expression after egg laying completely rescued the Ven phenotype (Figure 2D). (wormbook.org)

Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)

CD44-hyaluronate mediates in lymphocyte T and monocyte adhesion to the endothelium, stimulates proinflammatory cytokine release from macrophages and participates in dedifferentiation phenotype of smooth muscle cells from contractile state to synthetic one. (novoprolabs.com)

D) Injection of the XProfilin2 MO but not XProfilin1 MO or a control MO inhibits translation of Myc -tagged 5′UTR- XProfilin2 . (xenbase.org)

We cloned a genomic fragment that rescued the Ven phenotype (Figure 1A). (wormbook.org)
in which the C-terminus of VEN-1 in the rescue fragment was tagged with an influenza hemagglutinin (HA) peptide. (wormbook.org)
This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)

Jolkinolide B manufacture Markers for hESCs that may also contribute to the "stemness" phenotype have been established and markers that distinguish ESCs from embryoid bodies (EB) have been developed. (health-ground.com)

Sequences for all of the known enzymes involved in TIAs synthesis were identified. (biomedcentral.com)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)

The transient assay of selected genes resulted in a synergistic increase of EFA-enriched TAG accumulation in tobacco leaves. (researchsquare.com)

It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. (appliedbioinfo.com)
In the comma stage, it was expressed in hypodermal cells: Abpl/raapppp (H7), P1/2 L/R, P3/4 L/R, P5/6 L/R, P7/8 L/R, P9/10 L/R, and P11/12. (wormbook.org)
sequence rescued the Ven phenotype, and the HA epitope was detected in hypodermal cells (Figure 2B). (wormbook.org)

In this study, RNA-sequencing of pooled Uncaria capsules RNA samples taken at three developmental stages that accumulate different amount of RIN and IRN was performed. (biomedcentral.com)
While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. (westminster.ac.uk)

The identification of genes that were differentially expressed in the shell-forming mantle tissue and the comparison of the genotypes with the phenotypes under consideration of the inheritance of the characters is an appropriate approach to identify candidate genes that may be involved in the genetic mechanism resulting in the observed phenotypes. (peerj.com)
2001). Herein, we report our identification of the ven-1 gene through genetic/physical mapping as well as transformation rescue of the Ven phenotype. (wormbook.org)

Anatomy16

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