• it occurs in hypotonic solutions due to osmotic pressure and in isotonic solutions as a result of altered permeability of the membranes of respiring mitochondria. (harvard.edu)
  • Octyl gallate reduces ATP levels and Ki67 expression leading HepG2 cells to cell cycle arrest and mitochondria-mediated apoptosis. (harvard.edu)
  • A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc. (lookformedical.com)
  • Osmotic behavior and permeability of osmotically lysed mitochondria. (silverchair.com)
  • The ghosts have permeability properties and osmotic behavior comparable to those of isolated mitochondria. (silverchair.com)
  • The compartmentation and distribution of metabolites between mitochondria and the rest of the cell is a key parameter of cell signalling and pathology. (biomed.news)
  • Using this procedure revealed the in vivo compartmentation of mitochondrial metabolites and will enable assessment of the distribution of metabolites between the cytosol and mitochondria during a range of situations in vivo. (biomed.news)
  • Moreover, cell population measurements of mitochondrial and glycolytic metabolism using a Seahorse analyzer demonstrated that WT KRAS TP53-silenced cells display an increase of the basal respiration and tightly-coupled mitochondria, in the presence of glucose as substrate, compared to TP53 competent cells. (biomed.news)
  • Neuroglobin overexpression inhibits oxygen-glucose deprivation-induced mitochondrial permeability transition pore opening in primary cultured mouse cortical neurons. (harvard.edu)
  • Duality of effect of La3+ on mitochondrial permeability transition pore depending on the concentration. (harvard.edu)
  • MitoQ consequently decreased the mitochondrial membrane potential and mitochondrial superoxide production by these cells. (biomed.news)
  • Specifically, we explored how somatic mutations in these genes and substrate availability (lactate, glucose, substrate deprivation) from the extracellular microenvironment affect bioenergetic parameters, including cellular ATP, NADH and mitochondrial membrane potential dynamics. (biomed.news)
  • Employing cytosolic and mitochondrial FRET-based ATP probes, fluorescent NADH sensors, and the membrane-permeant cationic fluorescent probe TMRM in HCT-116 cells as a model system, we observed that TP53 deletion and KRAS mutations drive a shift in metabolic signatures enabling lactate to become an efficient metabolite to replenish both ATP and NADH following nutrient deprivation. (biomed.news)
  • Intriguingly, cytosolic, mitochondrial and overall cellular ATP measurements revealed that, in WT KRAS cells, TP53 deficiency leads to an enhanced ATP production in the presence of extracellular lactate and glucose, and to the greatest increase in ATP following a starvation period. (biomed.news)
  • Furthermore, cells possessing oncogenic KRAS, independently of TP53 status, showed less pronounced mitochondrial membrane potential changes in response to metabolic nutrients. (biomed.news)
  • Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
  • Plasma membrane depolarization reveals endosomal escape incapacity of cell-penetrating peptides. (uchicago.edu)
  • Using four different cancer cell lines, we report that, at clinically relevant concentrations (100-500 nM), MitoQ selectively repressed mesenchymal pancreatic cancer cell respiration, which involved the inhibition of the expression of PGC-1α, NRF1 and a reduced expression of electron-transfer-chain complexes I to III. (biomed.news)
  • Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles. (uchicago.edu)
  • After matching the models for the relative dispersion the remaining differences in relative skewness are predicted, discussing the relative roles of membrane permeability, cellular binding and cytoplasmic transport. (nih.gov)
  • Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. (lookformedical.com)
  • Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. (lookformedical.com)
  • On the other hand, oncogenic KRAS in TP53-deficient cells reversed the alterations in cellular ATP levels. (biomed.news)
  • It binds CHOLESTEROL and is involved in LIPIDS transport, membrane traffic, and SIGNAL TRANSDUCTION. (lookformedical.com)
  • Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. (lookformedical.com)
  • It serves as an internal transport system for moving essential substances throughout the cell, and in single-celled organisms, such as the AMOEBA, it is responsible for the movement (CELL MOVEMENT) of the entire cell. (bvsalud.org)
  • Reconstitution of eroded or injured EPITHELIUM by proliferation and migration of EPITHELIAL CELLS from below or adjacent to the damaged site. (jefferson.edu)
  • Growth differentiation factor 9 (GDF9) stimulates proliferation and inhibits steroidogenesis by bovine theca cells: influence of follicle size on responses to GDF9. (ouhsc.edu)
  • Somatic mutations in genes involved in oncogenic signaling pathways, including KRAS and TP53, rewire the metabolic machinery in cancer cells. (biomed.news)
  • We here set out to determine, at the single cell level, metabolic signatures in human colon cancer cells engineered to express combinations of activating KRAS gene mutations and TP53 gene deletions. (biomed.news)
  • The intracellular transfer of information (biological activation/inhibition) through a signal pathway. (lookformedical.com)
  • A caveolin that is expressed exclusively in MUSCLE CELLS and is sufficient to form CAVEOLAE in SARCOLEMMA. (lookformedical.com)
  • The results suggest that the assembly of the molecular components of the inner membrane is sufficient to specify the morphology. (silverchair.com)
  • Is phenol oxidation responsible for the short term effect of boron deficiency on plasma-membrane permeability and function in squash root? (udca.edu.co)
  • Revisiting the Latency of Uridine Diphosphate-Glucuronosyltransferases (UGTs)-How Does the Endoplasmic Reticulum Membrane Influence Their Function? (mdpi.com)
  • Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)
  • Phenotypically, MitoQ further inhibited pancreatic cancer cell migration, invasion, clonogenicity and the expression of stem cell markers. (biomed.news)
  • Naturally occurring ladderanes have been identified as major components of the anammoxosome membrane of the anammox bacteria, phylum Planctomycetota. (wikipedia.org)
  • Detergent-insoluble CELL MEMBRANE components. (lookformedical.com)
  • Four properties were found which have not been previously described in systems derived from biological membranes: (a) an osmotic behavior in the virtual absence of internal components. (silverchair.com)
  • Expression and effect of fibroblast growth factor 9 in bovine theca cells. (ouhsc.edu)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • Knockdown of MBP-1 in human prostate cancer cells delays cell cycle progression. (ouhsc.edu)
  • It reduced by ~50% the metastatic homing of human MIA PaCa-2 cells in the lungs of mice. (biomed.news)
  • The quantity of volume or surface area of CELLS. (ouhsc.edu)
  • Seres I, F?ris G, Varga Z, Koszt?czky B, Kassai A, Balogh Z, F?l?p P, Paragh G. The association between angiotensin II-induced free radical generation and membrane fluidity in neutrophils of patients with metabolic syndrome. (umassmed.edu)
  • Could angiotensin-II induced T-cell senescence exacerbate age-related vascular dysfunction? (ucdenver.edu)
  • Molecular determinants of endothelial transcytosis and their role in endothelial permeability. (rush.edu)
  • In addition, endothelial cell-specific NPRC knockout attenuated atherosclerotic lesions in mice. (bvsalud.org)
  • In contrast, endothelial cell overexpression of NPRC aggravated the size and instability of atherosclerotic aortic lesions in mice. (bvsalud.org)
  • Experiments in vitro showed that NPRC knockdown in human aortic endothelial cells (HAECs) inhibited ROS production, pro-inflammatory cytokine expression and endothelial cell apoptosis, and increased eNOS expression. (bvsalud.org)
  • Furthermore, NPRC knockdown in HAECs suppressed macrophage migration, cytokine expression, and phagocytosis via its effects on endothelial cells. (bvsalud.org)
  • On the contrary, NPRC overexpression in endothelial cells resulted in opposite effects. (bvsalud.org)
  • In conclusion, NPRC deletion reduced the size and instability of atherosclerotic lesions in ApoE-/- mice via attenuating inflammation and endothelial cell apoptosis and increasing eNOS expression by modulating cAMP/PKA-AKT1 and NF-κB pathways. (bvsalud.org)
  • Calcium has multiple effects on cell metabolism, membrane transport and permeability and, thus, on overall cell physiology or pathophysiology. (nih.gov)
  • cAMP and cGMP are important secondary messengers involved in cell regulation and metabolism driven by the G protein‑coupled receptor. (spandidos-publications.com)
  • Am J Physiol Lung Cell Mol Physiol. (rush.edu)
  • Asymmetric addition of ceramides but not dihydroceramides promotes transbilayer (flip-flop) lipid motion in membranes. (nih.gov)
  • Anderton CR, Lou K, Weber PK, Hutcheon ID, Kraft ML. Correlated AFM and NanoSIMS imaging to probe cholesterol-induced changes in phase behavior and non-ideal mixing in ternary lipid membranes. (umassmed.edu)
  • Reorganization of lipid domain structure in membranes by a transmembrane peptide: an ESR spin label study on the effect of the Escherichia coli outer membrane protein A signal peptide on the fluid lipid domain connectivity in binary mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine. (umassmed.edu)
  • Recent experiments in our laboratory document that EPXMA in combination with other biochemical and electrophysiological techniques can be used to study, for example, sodium and calcium compartmentation in cultured cardiac cells. (nih.gov)
  • As the multiple mitochondrial functions are mutually interconnected, changes in protein composition by mutations or in supercomplex assembly and/or in membrane structures often generate a dysfunctional cascade and lead to life-incompatible diseases or severe syndromes. (researchgate.net)
  • The level of cAMP is increased in association with cancer cell formation through activation of AC‑stimulatory G protein by mutation. (spandidos-publications.com)
  • Membrane Fluidity" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Cell Fusion" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • It may be secondary to one of the immunodeficiency syndromes, inherited as an autosomal recessive trait, or associated with defects in cell-mediated immunity, endocrine disorders, dental stomatitis, or malignancy. (doctorinternet.com)
  • The transport speed of 8-hydroxypyrene-1,3,6-trisulfonic acid in endosperm was 1.5 to 3 times faster than in cotyledon cells or Arabidopsis embryos. (springeropen.com)
  • Soybean endosperm cells allowed macromolecules to move fast via plasmodesmata transport. (springeropen.com)
  • It serves as an internal transport system for moving essential substances throughout the cell, and in single-celled organisms, such as the AMOEBA , it is responsible for the movement ( CELL MOVEMENT ) of the entire cell. (nih.gov)
  • Cells can be rapidly frozen for EPXMA during basal or altered functional conditions to delineate the location and amount of calcium within cells and the changes in location and concentration of cations or anions accompanying calcium redistribution. (nih.gov)
  • Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization. (jefferson.edu)
  • Such analyses can also be used to clarify the role of calcium in anoxic renal cell injury and to evaluate proposed ionic defects in cells of individuals with cystic fibrosis. (nih.gov)
  • Different effects of enzyme-generated ceramides and diacylglycerols in phospholipid membrane fusion and leakage. (nih.gov)
  • However, several plant families, such as Oenograceae, Cabombaceae, and Illiciaceae, form diploid endosperm originating from a central cell with only one polar nucleus (Williams and Friedman 2002 ). (springeropen.com)
  • J. D. Sharer, J. F. Shern, H. Van Valkenburgh and D. C. Wallace and R. A. Kahn, "ARL2 and BART Enter Mitochondria and Bind the Adenine Nucleotide Transporter," Molecular Biology of the Cell, Vol. 13, No. 1, 2002, pp. 71-83. (scirp.org)
  • Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. (lookformedical.com)
  • Soybean endosperm cells allowed fusion proteins (42 kDa) to move from bombarded cells to adjacent unbombarded-cells. (springeropen.com)
  • Such movement is not simple diffusion because the fusion proteins failed to move into dead cells. (springeropen.com)
  • Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE. (rush.edu)
  • Below are the most recent publications written about "Cell Fusion" by people in Profiles. (jefferson.edu)
  • Fusion of HepG2 cells with mesenchymal stem cells increases cancer-associated and malignant properties: an in vivo metastasis model. (jefferson.edu)
  • A stem cell fusion model of carcinogenesis. (jefferson.edu)
  • Paumet F. [Cell fusion]. (jefferson.edu)
  • Reconstitution of eroded or injured EPITHELIUM by proliferation and migration of EPITHELIAL CELLS from below or adjacent to the damaged site. (uchicago.edu)
  • Essential in an introductory consideration of microanalysis in biological cryosections is the physical basis for the instrumentation, fundamentals of X-ray spectrometry, and various analytical modes such as static probing and X-ray imaging. (nih.gov)
  • Senescent cells limit p53 activity via multiple mechanisms to remain viable. (ucdenver.edu)
  • Naturally occurring ladderanes have been identified as major components of the anammoxosome membrane of the anammox bacteria, phylum Planctomycetota. (wikipedia.org)
  • This graph shows the total number of publications written about "Membrane Fluidity" by people in this website by year, and whether "Membrane Fluidity" was a major or minor topic of these publications. (umassmed.edu)
  • Major components of the oils determine the biological activity of the oils however the components in smaller amounts produce an additive effect(Bakkali et al, 2007). (ukessays.ae)
  • Moreover, the morphology of mitochondria rules cell fate and the formation of the mitochondrial permeability transition pore in the mtIM, which, most likely with the F1FO-ATP synthase contribution, permeabilizes mitochondria and leads to cell death. (researchgate.net)
  • Molecular simulation of rapid translocation of cholesterol, diacylglycerol, and ceramide in model raft and nonraft membranes. (nih.gov)
  • Cytobiological observation showed that soybean endosperm cells were alive with zigzag-shape cell wall. (springeropen.com)
  • For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall. (lookformedical.com)
  • The minimum detectable concentration of calcium in biological cryosections is approximately 300 mumole kg dry weight with a spatial resolution of approximately 100 A. The application of electron energy loss (EELS) techniques to the detection of calcium offers the potential for greater sensitivity and spatial resolution in measurement and imaging. (nih.gov)
  • Seeds of approximately 70% flowering plants have endosperm cells (Brown and Lemmon 2007 ), most of them are triploidy. (springeropen.com)
  • Process by which cells irreversibly stop dividing and enter a state of permanent growth arrest without undergoing CELL DEATH. (ucdenver.edu)
  • The size exclusion limit is larger for soybean endosperm cells than its cotyledon or even Arabidopsis embryo cells. (springeropen.com)
  • p21 produces a bioactive secretome that places stressed cells under immunosurveillance. (ucdenver.edu)