• The effect of PSK, a protein-bound polysaccharide and an immunomodulator, on lymphocytes was examined in vitro for 36 patients with gastric cancer and 26 with colorectal cancer. (unboundmedicine.com)
  • Our findings thus show that the in vitro activation of lymphocytes by PSK can help identify the candidates with either gastric or colorectal cancer who are the best suited to undergo immunochemotherapy including treatment with PSK. (unboundmedicine.com)
  • Synergy among thymocytes and peripheral lymph node cells in the in vitro generation of lymphocytes cytotoxic to alloantigens. (ox.ac.uk)
  • The present results indicate that ZnO nanoparticles are clastogenic, mutagenic and cytotoxic to human lymphocytes in vitro at specific concentrations and time periods. (comu.edu.tr)
  • There is also evidence for the induction of CD4 expression and susceptibility to infection by HIV-1 of CD8 lymphocytes activated in vitro. (ox.ac.uk)
  • The aim of this study was to investigate the activity of Kombucha on human peripheral blood lymphocytes in vitro. (kombuchabrewers.org)
  • In an in vitro micronucleus assay in cultured peripheral human lymphocytes performed according to OECD Guideline 487, it was concluded that T003063 was not clastogenic or aneugenic in human lymphocytes in the absence and in the presence of S9 -mix under the experimental conditions described in the report. (europa.eu)
  • These cells inhibited T cell proliferation in a standard in vitro mixed lymphocyte assay and, moreover, attenuated the development of vasculopathy mediated by autologous peripheral blood mononuclear cells in a functionally relevant humanized mouse transplant model. (lu.se)
  • Tumor infiltrating lymphocytes and PBLs were phenotyped by their surface markers, cytokine pattern, proliferation rate, and cytotoxic ability. (nih.gov)
  • The specific targeting of responding CD8 lymphocytes may provide a functional explanation for the previously observed impairment of cytotoxic T-lymphocyte (CTL) function disproportionate to their numerical decline in AIDS and for the deletion of specific clones of CTLs responding to HIV antigens. (ox.ac.uk)
  • Moreover, direct ex vivo SIV-specific cytotoxic activity could be detected in PBMC from five of the six DNA/MVA-vaccinated animals, indicating that this epitope-based DNA prime/MVA boost regimen represents a potent method for inducing high levels of functionally active, Ag-specific CD8+ T lymphocytes in non-human primates. (ox.ac.uk)
  • Experimental treatment approaches include vaccines and nonmyeloablative allogeneic peripheral blood stem cell transplantation. (medscape.com)
  • Lymphocyte subsets in peripheral blood and gut microbiota composition in faeces samples were studied at the age of 4 months by flow cytometry analysis and qPCR respectively. (cambridge.org)
  • The level of The infants with PEM were enrolled in T-lymphocyte subsets in peripheral blood the study after fulfilling a set of inclusion provide information about the development criteria. (who.int)
  • In colorectal biopsies and peripheral blood lymphocytes of patients with IBD, polyps and CRC, we evaluated 1) genomic instability using the γH2AX assay as marker of DSB and micronuclei in mononuclear lymphocytes kept under cytodieresis inhibition, and 2) cellular stress through expression and cellular localization of glutathione-S-transferase omega 1 (GSTO1). (oncotarget.com)
  • To investigate the relationship between CD8 activation and infection by HIV-1 in vivo, activated subsets of CD8 lymphocytes in peripheral blood mononuclear cells (PBMCs) of HIV-seropositive individuals were investigated for CD4 expression and HIV infection. (ox.ac.uk)
  • We sought to determine what type of IGF receptor was present on peripheral blood mononuclear cells and which types of cells possessed these receptors. (utmb.edu)
  • Two color flow cytometric analysis of mononuclear cells stained with mouse monoclonal antibodies specific for the human IGF-I receptor, the human insulin receptor, and monoclonal antibodies directed against specific monocyte and lymphocyte subset cell surface antigens revealed that both IGF-I receptors and insulin receptors were present on nearly all monocytes and B-lymphocytes, but were present on only 2% of T-lymphocytes. (utmb.edu)
  • We conclude from these data that among human peripheral blood nonactivated mononuclear cells, IGF-I binds to specific type I IGF receptors found predominately on monocytes and B-lymphocytes. (utmb.edu)
  • Previous studies have suggested that specific IGF-I receptors are present on peripheral blood leukocytes, including polymorphonuclear leukocytes, lymphocytes, and monocytes. (utmb.edu)
  • Consequently, a widely validated body of macrophage turns into foam cells, and white blood cells notably monocytes and lymphocytes migrate to the intermedia of the vessel, releasing inflammatory factors into the blood stream, namely, Interleukin- (IL-) 1 β , IL-4, IL-6, and IL-10. (hindawi.com)
  • Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
  • To determine the immunologic characteristics of tumor infiltrating lymphocytes (TILs) in comparison with the corresponding peripheral blood lymphocytes (PBLs) of patients with ovarian cancer in order to detect specific antitumor-reactive-immunocompetent cells. (nih.gov)
  • Peripheral blood lymphocytes had a higher proliferative activity and cytotoxicity against natural killer-sensitive tumor cells than the corresponding TILs. (nih.gov)
  • Cultured lymphocytes with PSK at 100 micrograms/ml increased the level of DNA synthesis, as determined by the 3H-thymidine uptake, from 0.9 to 3.0 fold, compared to the PSK non-treated cells. (unboundmedicine.com)
  • To investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and cutaneous warts lesions. (fiocruz.br)
  • HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • Subset analysis of the engrafted cells using a multiparametric system enabling a combined analysis of morphology, immunophenotyping and FISH showed that both T and B lymphocytes and myeloid cells were of donor origin in two patients, while T lymphocytes and myeloid cells were of donor origin in the third. (nih.gov)
  • Herein, we investigated the gene expression profile in peripheral B-cells, as well as a panel of inflammatory serum proteins, in 30 FM patients and 23 healthy matched control individuals. (figshare.com)
  • Reverse transcriptase (RT)-polymerase chain reaction (PCR) analysis revealed the dominant expression of caspase-8L transcript compared to the intact form of caspase-8 in human peripheral blood lymphocyte (PBL) and T cells. (elsevierpure.com)
  • While the autoreactive cells displayed significantly reduced Tregs numbers, the alloreactive transplacentally acquired maternal lymphocytes had high functional Tregs. (hindawi.com)
  • In some cases, T cells may present, as a result of residual autologous cells or transplacentally acquired maternal lymphocytes [ 1 ]. (hindawi.com)
  • The origin of the reactive cells in such patients are either thymic release of T-cells that expand at the periphery or transplacentally transfers of maternal T lymphocytes. (hindawi.com)
  • These cells expand in the periphery, causing tissue infiltration and damage due to breakdown of both central (e.g., autoimmune regulator, AIRE protein dysfunction) and peripheral (FOXP3 + deficiency) tolerance mechanisms [ 3 ]. (hindawi.com)
  • For example, Omenn syndrome, a typical case of impaired T-cell differentiation with abnormal self-reactive cells, is invariably characterized by autoimmune features such as generalized scaly exudative erythroderma, enlarged lymphoid tissues, and peripheral expansion of oligoclonal T-cells, in addition to increased susceptibility for severe infections [ 3 , 4 ]. (hindawi.com)
  • A distinctive feature of SCID patients, which sometimes can clinically resemble Omenn, [ 6 ] is the presence of alloreactive cells originated from transplacentally maternal T lymphocytes. (hindawi.com)
  • HIV-1 may infect thymic precursor cells destined to become CD4 and CD8 lymphocytes and contribute to the numerical decline in both subsets on disease progression. (ox.ac.uk)
  • CD4(+) and CD4(-) CD8 lymphocytes, CD4 lymphocytes, other T cells, and non-T cells were purified using paramagnetic beads, and proviral sequences were quantified by PCR using primers from the long terminal repeat region. (ox.ac.uk)
  • Frequencies of activated CD8 lymphocytes were higher in HIV-infected study subjects than in seronegative controls, and they frequently coexpressed CD4 (mean frequencies on CD69(+), CD71(+), and HLA class II(+) cells of 23, 37, and 8%, respectively, compared with 1 to 2% for nonactivated CD8 lymphocytes). (ox.ac.uk)
  • A productive infection of peripheral blood lymphocytes by HIV-1 was severely inhibited by the simultaneous infection of these cells with HIV-2. (biu.ac.il)
  • Their peripheral blood lymphocytes (PBL) harvested at different days after vaccination showed specific activity against target cells infected with vaccinia virus with a peak activity at day 7. (silverchair.com)
  • Fig. 5: Change in phenotype and genotype of peripheral T cells after TIL infusion. (nature.com)
  • TRV-β sequencing of tumor and peripheral T cells as the project 'TIL trial for NSCLC' are available in the immuneACCESS free public database at https://clients.adaptivebiotech.com/immuneaccess . (nature.com)
  • Regarding lymphocyte subpopulations, even though CD4+ T lymphocytes and B cells were depleted around 4dpi in both infected groups, the main difference observed between both groups was in CD8+ T cells which displayed an earlier depletion in BVDV inoculated calves that can promote a greater BHV-1 dissemination, thus aggravating the course of the disease. (unimi.it)
  • The correlation between the peripheral blood cells aggregation level measured by surface plasmon resonance method (SPR), and chromosomal abberations of peripheral blood lymphocytes in brain malignant gliomas patients was investigated. (rudn.ru)
  • Changes in the percentage of peripheral blood CD 19 positive lymphocytes (B cells) correlated with changes in disease activity (Spearman rank coefficients = 0.47, p = 0.02). (northwestern.edu)
  • Change in the percentage of peripheral blood B cells correlates with change in disease activity in patients with JDM. (northwestern.edu)
  • The aim of our research was to determine how immunotherapy changes the proportion of lymphocyte subsets in dog peripheral blood and the levels of cytokines secreted by these cells during therapy. (mdpi.com)
  • and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)
  • about 75% of the lymphocytes are T cells, 20% B cells, and 5% NK cells. (msdmanuals.com)
  • Effect of infection with BHV-1 on peripheral blood leukocytes and lymphocyte subpopulations in calves with subclinical BVD / V. Molina, M.A. Risalde, P.J. Sánchez-Cordón, M. Pedrera, F. Romero-Palomo, C. Luzzago, J.C. Gómez-Villamandos. (unimi.it)
  • If the complete blood count reveals lymphocytopenia, testing for immunodeficiency and analysis of lymphocyte subpopulations should follow, usually after the patient has recovered from any acute event. (msdmanuals.com)
  • Peripheral blood CD4 but not CD8 t-lymphocytes in patients with exacerbation of asthma transcribe and translate messenger RNA encoding cytokines which prolong eosinophil survival in the context of a Th2-type pattern: effect of glucocorticoid therapy. (atsjournals.org)
  • Successful stem cell transplantation for patients with severe combined immunodeficiency (SCID) from matched family donors without conditioning results in engraftment of T lymphocytes. (nih.gov)
  • We studied three SCID patients who were transplanted with unmodified mobilized peripheral blood from HLA-identical family sex-mismatched members. (nih.gov)
  • MART-1 is an Ag expressed on melanomas and melanocytes, and is recognized by the majority of HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes (TIL) from melanoma patients. (elsevierpure.com)
  • It was shown that decreasing of SPR data correlate with the statistically significant increase of chromosomal aberration in peripheral blood lymphocytes in glioma patients. (rudn.ru)
  • Eisenstein, DM, O'Gorman, MRG & Pachman, LM 1997, ' Correlations between change in disease activity and changes in peripheral blood lymphocyte subsets in patients with juvenile dermatomyositis ', Journal of Rheumatology , vol. 24, no. 9, pp. 1830-1832. (northwestern.edu)
  • 56: 1-6 (1976) The Distribution of B and T Lymphocytes in the Peripheral Blood of Patients with Hodgkin s Disease Amira Many, M iriam Biniaminov, E sther Rosenthal, E sther Aghai and Bracha R amot The Chaim Sheba Medical. (karger.com)
  • CD4+ T-lymphocyte levels are also used as prognostic indicators in patients with human immunodeficiency virus (HIV) disease (8). (cdc.gov)
  • Enhanced apoptosis in the leukocytes of peripheral blood of PEM patients may be a marker of increased infection and immune disturbances. (who.int)
  • In this study, possible genotoxic effects of zinc oxide (ZnO) nanoparticles were investigated in cultured human peripheral lymphocytes by using chromosome aberrations and micronucleus assays (MN). (comu.edu.tr)
  • Activated peripheral CD8 lymphocytes express CD4 in vivo and are targets for infection by human immunodeficiency virus type 1. (ox.ac.uk)
  • There is increasing evidence that CD8 lymphocytes may represent targets for infection by human immunodeficiency virus type 1 (HIV-1) in vivo whose destruction may contribute to the loss of immune function underlying AIDS. (ox.ac.uk)
  • Activated CD8 lymphocytes were identified by expression of CD69, CD71, and the human leukocyte antigen (HLA) class II, the beta-chain of CD8, and the RO isoform of CD45. (ox.ac.uk)
  • Human peripheral blood lymphocytes (PBLs) were from mice infected with a SAg-producing S. pyogenes purified from blood of a healthy donor by using strain (29). (cdc.gov)
  • In tubes several parameters affecting the colony formation of mouse and human granulocytes and macrophages as well as of human peripheral T and B lymphocyte from mouse spleen were optimized. (karger.com)
  • Human lymphocytes T lymphocytes B lymphocytes Mouse ro- sette Lymphoproliférative diseases Abstract. (karger.com)
  • 57: 247-256 (1977) Scanning Electron Microscopic Study of Leukemic Human B Lymphocytes K. F. (karger.com)
  • This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • Cholesterol synthesis in cultured human peripheral lymphocytes. (bvsalud.org)
  • Human Immunodeficiency Virus (HIV) Infection Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain. (msdmanuals.com)
  • Selective disbalances of peripheral blood T lymphocyte subsets in human CD3 gamma deficiency. (lu.se)
  • To investigate whether changes in peripheral blood lymphocytes correlate with changes in disease activity in juvenile dermatomyositis (JDM). (northwestern.edu)
  • It is important to note that the lymphocytes in the blood represent only a small proportion of the total lymphocyte pool, and the lymphocyte count may not always correlate with the composition and numbers of lymphocytes in other lymphoid (eg, nodes, spleen) and non-lymphoid (eg, lung, liver) tissues. (msdmanuals.com)
  • B lymphocytes Immunofluorescence Lymphatic. (karger.com)
  • There were 18 non-oedematous infants (8 described in peripheral blood and lymphatic males and 10 females) with mean age 10.78 organs during infection [ 9 ]. (who.int)
  • Specifically, in order to assess the potential impacts of P. falciparum infections either during pregnancy or during infancy, we quantified lymphocyte subsets in cord blood and in infants' peripheral blood during the first year of life. (unibo.it)
  • We conclude that maternal P. falciparum infection at delivery has significant and, in some cases, year-long effects on the composition of infants' peripheral blood lymphocyte populations. (unibo.it)
  • We sought to assess the effect of several early environmental factors on lymphocyte subsets and microbiota composition in infants at familial risk for CD. (cambridge.org)
  • Linear regression analysis showed that, the absolute counts of total lymphocytes, CD3+, CD4+, CD4+CD38+, CD4+CD28+, were positively associated with formula-fed infants and infant's infections in the first 4 months of age. (cambridge.org)
  • All enrolled thymic lymphocytes is widely accepted as infants were breastfed in addition to receiv- an indicator of the depression of thymus- ing some traditional foods, according to dependent immune competence associated their age. (who.int)
  • A low ratio of CD4+ (helper) any chromosomal or hereditary disorder lymphocytes relative to CD8+ (suppressor) that caused the malnutrition. (who.int)
  • Neoantigen-reactive T cell clonotypes increased and persisted in peripheral blood after treatment. (nature.com)
  • Lymphocytopenia may also reflect impaired lymphocyte production arising from destruction of thymic or lymphoid architecture. (msdmanuals.com)
  • Combined, these findings provide evidence that antigenic stimulation of CD8 lymphocytes in vivo induces CD4 expression that renders them susceptible to HIV infection and destruction. (ox.ac.uk)
  • Micronuclei frequency began to rise in lymphocytes of subjects with polyps, suggesting a systemic genomic instability condition. (oncotarget.com)
  • T-lymphocyte (T-LC)-derived cytokines have been implicated in asthma pathogenesis. (atsjournals.org)
  • Activation of peripheral blood CD4 but not CD8 T-LC and a Th2-type pattern of elevated cytokine mRNA expression in BAL fluid T-LC have been observed in asthmatics, but the principal source (CD4 or CD8 T-LC) of these cytokines is unknown. (atsjournals.org)
  • We conclude that peripheral blood CD4 but not CD8 T-LC from asthmatics express cytokine mRNA in a Th2-type pattern and show elevated secretion of cytokines prolonging eosinophil survival. (atsjournals.org)
  • Levels of Helper T lymphocytes and serum anti-inflammatory cytokines involving IL-37 and IL-38 were all elevated, and the depressive behaviors of HFD+UCMS rats were attenuated by GBE. (hindawi.com)
  • The phenotypes of both lymphocyte populations were very heterogeneous. (nih.gov)
  • The suggested mechanism for this phenomenon is the possible inability of the thymus to delete these abnormal clones due to compromise of both central and peripheral tolerance mechanisms [ 5 ]. (hindawi.com)
  • The input to the pharmacokinetic model was a lognormal1y-distributed, autocorre1ated series of exposure concentrations while the output consisted of the resulting concentrations of styrene in the central and peripheral compartments. (cdc.gov)
  • The pathogenesis of acquired immunodeficiency syndrome (AIDS) is largely attributable to the decrease in T-lymphocytes bearing the CD4 receptor (CD4+) (1-5). (cdc.gov)
  • El ligando es específico para el RECEPTOR 4-1BB y puede desempeñar un papel en la indución de la proliferación de los LINFOCITOS T activados de la sangre periférica. (bvsalud.org)
  • The ligand is specific for the 4-1BB RECEPTOR and may play a role in inducing the proliferation of activated peripheral blood T-LYMPHOCYTES. (bvsalud.org)
  • Brief report: primary immunodeficiency caused by mutations in the gene encoding the CD3-gamma subunit of the T-lymphocyte receptor. (lu.se)
  • As expected, cortisol decreased basal and mitogen-induced lymphocyte proliferation in a dose dependent manner (P (usda.gov)
  • Methods: Peripheral lymphocyte lysates were analysed for Poly(ADP-Ribose) Polymerase activity, and by SDS-PAGE and anti-Poly(ADP-Ribose)Polymerase 1-antibody to measure automodified anti-Poly(ADP-Ribose) Polymerase levels by densitometry. (preprints.org)
  • The peripheral blood lymphocyte culture is one of the most routinely used and traditional methods for karyotyping analysis. (geneticeducation.co.in)
  • To assure the accuracy and reliability of CD4+ T-lymphocyte test results obtained within individual laboratories and to assure the comparability of results between laboratories, standard methods for performing the test, as well as guidelines for quality control and quality assurance, are desirable. (cdc.gov)
  • Adoptive cell therapy using tumor-infiltrating lymphocytes (TILs) has shown activity in melanoma, but has not been previously evaluated in metastatic non-small cell lung cancer. (nature.com)
  • The results did not show relevant differences in either T or B lymphocytes from mice exposed to an SAR of 1 or 2 W/kg and sham-exposed mice with few exceptions. (bioone.org)
  • 3 3 2009 B lymphocytes Cell surface Hairy cell leukemia Leukemia Malignant lymphomas Scanning electron microscopy Acta haemat. (karger.com)
  • Our objective was to measure expression of Th1- and Th2-type cytokine mRNA and spontaneous secretion of IL-3, IL-5, and GM-CSF by peripheral blood CD4 and CD8 T-LC from asthmatics before and after oral glucocorticoid therapy and non-asthmatic controls. (atsjournals.org)
  • However, the contribution of different components in the immune system, such as the B-lymphocytes, in the progression to FM are yet unknown. (figshare.com)
  • Because lymphocytes account for only 20 to 40% of the total white blood cell (WBC) count, lymphocytopenia may go unnoticed if the WBC count is checked without a differential. (msdmanuals.com)
  • This study suggests that GBE poses a protective role from the various pathologies associated with high-fat diets, unpredictable chronic mild stress, and depression, possibly via improving peripheral immunity and reducing cardiac and brain inflammation. (hindawi.com)
  • In conclusion, the balance between the effects of the pre- and post-natal factors on lymphocyte subsets and microbiota composition might modify the risk for future development of immune-related diseases such as celiac disease. (cambridge.org)
  • T-lymphocyte counts, with special emphasis and stunted children has not changed dra- on the effect of nutritional rehabilitation. (who.int)
  • We suggest that a high-tolerance capacity of the alloreactive transplacentally acquired maternal lymphocytes represents a toleration advantage, yet still associated with severe immunodeficiency. (hindawi.com)
  • Storage Compact disc4+ Testosterone levels lymphocytes in peripheral bloodstream that express integrins 4?7 preferentially recirculate through gut-associated lymphoid tissues (GALT), a proposed site of significant HIV-1 duplication. (bio2009.org)
  • The Cambridge Biotech HIV-1 Western Blot Kit is manufactured by Calypte Corporation from HIV-I propagated in an H9/HTLV-IIIb T-lymphocyte cell line. (cdc.gov)
  • Components and Strategies Individuals Eight treatment-naive topics with recorded chronic HIV-1 illness (CHI) and fairly high Compact disc4+ Capital t cell matters in peripheral bloodstream had been included in this Rabbit Polyclonal to DQX1 research (Desk 1). (bio2009.org)
  • We investigated whether these two markers (increased catalytic activity and auto modification), could help to monitor DNA damage in lymphocytes of flower growers from Southern Italy, occupationally exposed to pesticides. (preprints.org)
  • Conclusions: PARP activity and auto-modification in peripheral blood lymphocytes are possible,non-invasive, and routinar tools to monitor the healthy conditions of floricoltorists. (preprints.org)
  • Induction of AIDS virus-specific CTL activity in fresh, unstimulated peripheral blood lymphocytes from rhesus macaques vaccinated with a DNA prime/modified vaccinia virus Ankara boost regimen. (ox.ac.uk)
  • Clinical changes in disease expression measured by a disease activity score were correlated with changes in percentages of peripheral blood lymphocyte subsets. (northwestern.edu)
  • We established conditions for inducing antigen-specific tolerance in T h 2 lymphocytes by means of oral tolerance. (huji.ac.il)
  • Conditions for generating T h 2 lymphocyte tolerance were different from those required to generate tolerance in T h 1 lymphocytes: these included extended, continuous exposure to high dosages of antigen, rather than a single or intermittent feeding regimen which was sufficient to induce tolerance in T h 1 lymphocytes. (huji.ac.il)
  • These findings suggest that continuous oral exposure to a tolerogen may be a biologically relevant strategy to tolerize both T h 1- and T h -dependent responses, and extend the potential clinical use of oral tolerance to ailments mediated by T h 2 lymphocytes. (huji.ac.il)
  • Influence of early environmental factors on peripheral. (cambridge.org)
  • Kombucha from camellia sinensis and camellia sinensis substrate increased frequency of MN and SCE on mitomycin C-treated and -untreated peripheral blood lymphocytes. (kombuchabrewers.org)
  • However, Kombucha from Satureja montana reduced the incidence of MN on mitomycin C-treated and -untreated peripheral blood lymphocytes, while SCE frequency was higher than the control value. (kombuchabrewers.org)