Pericytes human skeletal muscle satellite cells skeletal muscle. Medical search. Frequent questions

Transplanted myoblasts can migrate several millimeters to fuse with damaged myofibers in nonhuman primate skeletal muscle. (medecinesciences.org)
Long-term engraftment (16 years) of myoblasts in a human infarcted heart. (medecinesciences.org)
More specifically, upon activation, satellite cells can re-enter the cell cycle to proliferate and differentiate into myoblasts. (wikipedia.org)
Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
Skeletal myoblasts are available from both male and female donors. (zen-bio.com)
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)

Fibroblasts within the muscle deposit scar tissue, which can impair muscle function, and is a significant part of the pathology of muscular dystrophies. (wikipedia.org)
Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. (neuromuscularnetwork.ca)

1999). such as platelets, macrophages, fibroblasts, epithelial The effect of anabolic steroids appears due to an cells, and endothelial cells. (sagepub.com)
Using single-cell RNA sequencing, we uncover distinct sclerotome-derived fibroblast populations in zebrafish, including progenitor-like perivascular/interstitial fibroblasts, and specialized fibroblasts such as tenocytes. (bvsalud.org)
By combining single-cell clonal analysis and live imaging, we demonstrate that perivascular/interstitial fibroblasts actively migrate to the injury site, where they proliferate and give rise to new tenocytes. (bvsalud.org)
By contrast, perivascular fibroblast-derived pericytes or specialized fibroblasts, including tenocytes, exhibit no regenerative plasticity. (bvsalud.org)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)
These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. (medecinesciences.org)
Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. (wikipedia.org)

Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)
Fin injury triggers wound epidermis formation and the dedifferentiation of injury-adjacent mature cells to establish an organized blastema of progenitor cells. (bvsalud.org)

A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)

The field of satellite cell biology suffers from the same technical difficulties as other stem cell fields. (wikipedia.org)
Her research investigates mechanisms of muscle stem cell dysfunction in neuromuscular disorders, with the aim of transferring this knowledge towards future cell and gene therapies. (neuromuscularnetwork.ca)
She completed her PhD in Dr. Peter Zammit's lab at King's College London (UK), where she investigated muscle stem cell regeneration in facioscapulohumeral muscular dystrophy (FSHD). (neuromuscularnetwork.ca)
She then spent 3 years completing a postdoc with Dr. Francesco Saverio Tedesco at University College London (UK) in stem cell trans-differentiation and disease modelling. (neuromuscularnetwork.ca)
This research aims to understand how mechanosensing via the LINC complex contributes to the process muscle stem cell repair, with relevance to Emery-Dreifuss muscular dystrophy. (neuromuscularnetwork.ca)
Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells. (neuromuscularnetwork.ca)
Stem Cell Reports. (neuromuscularnetwork.ca)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)

WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)

Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
The process of muscle regeneration involves considerable remodeling of extracellular matrix and, where extensive damage occurs, is incomplete. (wikipedia.org)
In this study, the level of proenkephalin-A (PENK-A), expressed by renal proximal tubular epithelial cells, decreases significantly with the loss of renal proximal tubules and increased at the termination phase of zebrafish kidney regeneration. (bvsalud.org)

Moreover, both quiescent and activated human satellite cells can be identified by the membrane-bound neural cell adhesion molecule (N-CAM/CD56/Leu-19), a cell-surface glycoprotein. (wikipedia.org)

Intramuscular transplantation of myogenic cells in primates: importance of needle size, cell number, and injection volume. (medecinesciences.org)
Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)
Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities. (medecinesciences.org)
These dividing cells are known as the "transit amplifying pool" before undergoing myogenic differentiation to form new (post-mitotic) myotubes. (wikipedia.org)
Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling. (neuromuscularnetwork.ca)

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)

However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
Limitations in the usage of AAV in murine models include cell types that are refractory to transduction and difficulties achieving tissue-specificity. (nature.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
The term 'cluster' refers to a cluster of single cells, which usually represents a cell or tissue type. (ucsc.edu)

Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy. (medecinesciences.org)

As such, the satellite cell niche is relatively ill-defined and it is likely that it consists of multiple sub-populations. (wikipedia.org)
Tedesco, FS, Moyle, LA, Perdiguero, E. Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle. (neuromuscularnetwork.ca)

However, Pax7 is expressed prominently after satellite cell differentiation. (wikipedia.org)
One of the first roles described for IGF-1 was its involvement in the proliferation and differentiation of satellite cells. (wikipedia.org)
All cells have been tested for myotube differentiation. (zen-bio.com)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

In addition, IGF-1 expression in skeletal muscle extends the capacity to activate satellite cell proliferation (Charkravarthy, et al. (wikipedia.org)
Balanced cell proliferation and redifferentiation along with cell movements then progressively reestablish patterned tissues and restore the fin to its original size and shape. (bvsalud.org)

Using human retinal endothelial cells in 20 mM D-glucose, H3K9me3 at Rac1 promoter was quantified by chromatin-Immunoprecipitation technique. (nature.com)

Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)

Here we generate a robust system for in vivo transgene delivery by combining the versatility of AAV-mediated transgene delivery with the specificity of cell-type-specific Cre-mediated recombination. (nature.com)
The Spotahome Blog implements of only several were 750- 323 BC: A ResearchGate example Shows an many proceedings to the possible number of vivo muscle for all fields of eddies, from cytosol to competitive error. (cdmw.de)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

The data allow for direct and controlled comparison of gene expression in cell types shared between tissues, such as immune cells from distinct anatomical locations. (ucsc.edu)

Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Myosatellite cells are located between the basement membrane and the sarcolemma of muscle fibers, and can lie in grooves either parallel or transversely to the longitudinal axis of the fibre. (wikipedia.org)
Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)

There is also evidence suggesting that these cells are capable of fusing with existing myofibers to facilitate growth and repair. (wikipedia.org)
Satellite cells proliferate following muscle trauma and form new myofibers through a process similar to fetal muscle development. (wikipedia.org)

Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)

Mol Biol Cell. (neuromuscularnetwork.ca)

Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)

Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions. (neuromuscularnetwork.ca)

Tabula Muris is a compendium of single cell transcriptome data from the model organism Mus musculus , containing nearly 100,000 cells from 20 organs and tissues. (ucsc.edu)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
This theory has its origins in the concept of "Wirkungssphäre" or "sphere of influence" proposed by Strassburger (1893) , in which he argued that a nucleus can only support a discrete volume of cytoplasm, thus defining the upper limits to cell size. (frontiersin.org)
Spindle cells are marked by their fusiform shape and eosinophilic cytoplasm. (medscape.com)

We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next-generation human artificial chromosomes for Duchenne muscular dystrophy. (neuromuscularnetwork.ca)

A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)

This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)

There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)

Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchenne muscular dystrophy dogs. (medecinesciences.org)
Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)
De novo revertant fiber formation and therapy testing in a 3D culture model of Duchenne muscular dystrophy skeletal muscle. (neuromuscularnetwork.ca)

By directing AAV transduction through the regulated expression of its receptor, the approach provides precise control over which cells express the AAV-vectored transgene. (nature.com)
A mechanistic understanding of these coordinated cell behaviors and transitions requires direct knowledge of proteins in their physiological context, including expression, subcellular localization, and activity. (bvsalud.org)
Furthermore, H2O2 influences the expression of tcf21, a vital factor in the formation of renal progenitor cell aggregates, by remodeling H3K4me3 in renal cells. (bvsalud.org)
A rectangle on the genome, at the location of a gene, filled with a bar graph that indicates the gene's expression by single cell cluster. (ucsc.edu)

After several cell divisions, the satellite cells begin to fuse with the damaged myotubes and undergo further differentiations and maturation, with peripheral nuclei as in hallmark. (wikipedia.org)

Studies rely almost exclusively on Flow cytometry and fluorescence activated cell sorting (FACS) analysis, which gives no information about cell lineage or behaviour. (wikipedia.org)
This track shows the results from FACS sorted cells sequenced with the SmartSeq2 protocol, as it has much higher transcript coverage. (ucsc.edu)

Anatomy17

Organisms2

Diseases2

Chemicals and Drugs12

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes13

Anthropology, Education, Sociology and Social Phenomena1

Myoblasts9

Muscular dystrophies2

Fibroblasts5

Adult3

Precursors2

Myoblast1

Progenitor cells2

Mesenchymal stem3

Stem Cell9

Adipocyte1

Regeneration5

Neural1

Myogenic5

Pluripotent stem1

Tissue8

IPSC-derived1

Populations2

Differentiation3

Acquired by a1

Proliferation2

Retinal1

Precursor1

Vivo3

Distinct1

Fibers3

Myofibers2

Placenta1

Biol1

Multipotent1

Self-renewal1

Organs1

Cytoplasm3

Tissues2

Progenitors1

Bone marrow1

Cartilage1

Hypertrophy1

Duchenne3

Expression4

Fuse1

FACS2