OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation Science
Random Amplified Polymorphic DNA TechniqueDNA, RecombinantDNA FingerprintingMolecular Sequence DataBase SequenceCloning, MolecularEscherichia coliPlasmids
PolymorphismRAPDFragment lengthAmplificationFragmentsGenomicMolecularTandemASSESSMENTNucleotideGenetic variabilityBandsPresenceSamplesIndexHealthElementsIndividuals1988GenesPrimersGenetic diversityMethylationRoutineSuccessfullyGenerateSequencesGenomeSpeciesMarkersClinicalPathwaysAnalysisReproductiveGene expressionMiceIdentificationHumanPositiveTimeAverage
Polymorphism3
  • The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)
  • alata ) using amplified fragment length polymorphism (AFLP) technique. (open-science-repository.com)
  • sessed, out of which 249 were polymorphic revealing 96.13% polymorphism. (scirp.org)
RAPD4
  • Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
  • Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
  • Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
  • 1984). Solanum demissum, phic DNA (RAPD) and restriction fragment length poly- a Mexican hexaploid species, has been used extensively morphism (RFLP) markers that are closely linked to the in potato breeding programs. (health-abstracts.com)
Fragment length1
  • Residual heterozygosity in the initial inbreds can partly explain the observed responses as evidenced by 42 markers derived from both Methyl-Sensitive Amplification- and Amplified Fragment Length- Polymorphisms. (biomedcentral.com)
Amplification1
  • DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
Fragments1
Genomic1
  • The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. (mabjournal.com)
Molecular1
  • 2000). The main advantage in this context is that the complex genetic and epigenetic correlations among interconnected parts of the brain can be explored using complementary molecular, developmental, structural, pharmacological, and behavioral techniques. (nervenet.org)
Tandem1
  • It's been approximated that to anticipate the ancestry of people, up to thousand random one nucleotide polymorphisms (SNPs) or brief tandem repeats (STRs) may 864814-88-0 manufacture be required [13]. (tech-strategy.org)
ASSESSMENT2
  • Mitochondrial DNA diversity of mud crab Scylla olivacea (Portunidae) in Peninsular Malaysia: a preliminary assessment. (mabjournal.com)
  • Provision of broad, method-based PT schemes, such as DNA sequencing, would allow assessment of a large number of tests for which formal PT is not currently available. (cdc.gov)
Nucleotide1
  • However, the replisome can be stalled by depletion of nucleotide pools or barriers on the template, such as DNA damage, secondary structures or protein complexes [ 11 ]. (biomedcentral.com)
Genetic variability1
  • These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. (biomedcentral.com)
Bands1
  • Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 Eco RI and Ms eI primer combinations. (open-science-repository.com)
Presence1
  • The presence of PCV2 DNA in tissue samples and the pathological lesions were more evident in pigs infected with both PCV2 and PPV than in pigs infected with PCV2 alone. (scielo.br)
Samples1
  • DNA samples (10 µg) were digested with Eco Rl, Hin dlll, Eco RV, These three BC populations are designated 1K6, 1K27 and LB1, or Dra l at a DNA/enzyme ratio of 7 units per microgram. (health-abstracts.com)
Index1
  • Mitotic index decreased, and the percentage of mitotic aberrations increased as the concentration of treatments increased and the time prolonged. (springeropen.com)
Health1
  • Most Neighborhood Healthcare health centers experienced an increase in the percentage of FIT kit returns (average of 3.6 percentage points) and individuals screened (an average increase of 111 FIT kits per month) from the baseline period through the implementation period. (cdc.gov)
Elements1
  • As originally proposed in 1963, cells rely on two genetic elements to duplicate their genome: the replicator, a DNA region where replication begins (now named the replication origin), and the initiator, a protein or a protein complex that recognizes the replicator [ 1 ]. (biomedcentral.com)
Individuals1
  • In conclusion, the results indicate that incentive programs can have an overall positive impact on both the percentage of FIT kits returned and the number of individuals screened. (cdc.gov)
19882
  • While the number of acute care beds in U.S. hospitals decreased between 1988 and 1998, the number of ICU beds increased. (medscape.com)
  • For download Uniqueness Theorems for Variational Problems by, GUDYKUNST and TING-TOOMEY( 1988) or BENNETT( 1998) shaped clashes of previous only policies. (music-of-benares.com)
Genes4
  • Genes are sequences of nucleotides in a particular segment (locus) of a DNA molecule. (scialert.net)
  • In contrast, "functional markers" (FMs) are derived from polymorphic sites within genes, quantitative trait nucleotides (QTN) or quantitative trait insertion - deletion mutations (QTINDEL) and have significant potential to translate genomic technologies into improved crop varieties. (biomedcentral.com)
  • Syvänen [ 8 ] has described five phases of functional marker development (1) functionally characterised genes, (2) allele sequences from such genes, (3) identification of polymorphic, functional motifs affecting plant phenotype within these genes, (4) validation of associations between DNA polymorphisms and trait variation, and (5) conversion into technical assays using, e.g., any of the single nucleotide polymorphism (SNP) or INDEL detection technologies. (biomedcentral.com)
  • Genes involved in cell cycle progression, DNA repair, cytoskeletal and extracellular matrix were frequently reported to be up-regulated in HCC while immune response and metabolic enzyme genes were found to be down-regulated [ 5 ]. (biomedcentral.com)
Primers2
  • SCoT technique was performed by using 11 primers to generate reproducible polymorphic products. (springeropen.com)
  • A PCR-based strategy with primers designed on exons was used to successfully amplify NPR1 gene fragments from different Vitaceae accessions. (beds.ac.uk)
Genetic diversity4
  • Genetic diversity is reflected in differences among individuals for many characters, including eye, skin and hair color in humans, color and banding patterns of snail shells, flowers in plants and protein and DNA sequences. (scialert.net)
  • Electrophoretic SDS-PAGE and SCoT-PCR techniques were used to study the fingerprints and genetic diversity of six Egyptian soybean ( Glycine max L.) genotypes (Giza111, Giza21, Giza82, Giza35, Giza22, and Giza83). (springeropen.com)
  • Deoxyribonucleic acid (DNA)-based markers were commonly used in studies of genetic diversity, comparative biology, morphological characters, environmental conditions, conservation, and phylogenic phenomena between plant species and cultivars (Haq et al. (springeropen.com)
  • More recently, molecular techniques have been extensively used in genetic diversity assessment, investigation of genetic relationships among different germplasm groups, marker development, identification of interspecific genome introgression, gene cloning and function analysis, and genetic transformation of important traits concerning productivity, quality and food safety of peanut. (peanutscience.com)
Methylation10
  • DNA methylation is a key epigenetic modification that can directly affect gene regulation. (biomedcentral.com)
  • DNA methylation is highly influenced by environmental factors such as cigarette smoking, which is causally related to chronic obstructive pulmonary disease (COPD) and lung cancer. (biomedcentral.com)
  • To date, there have been few large-scale, combined analyses of DNA methylation and gene expression and their interrelations with lung diseases. (biomedcentral.com)
  • Our results provide a large and comprehensive association study of whole blood DNA methylation with gene expression. (biomedcentral.com)
  • The low reproducibility of trans CpG-transcript pairs suggests that DNA methylation regulates nearby rather than remote gene expression. (biomedcentral.com)
  • DNA methylation was long thought to inhibit gene expression [ 2 ]. (biomedcentral.com)
  • Moreover, DNA methylation is determined by a complex interplay of genetic and environmental factors. (biomedcentral.com)
  • Mendelian randomization (MR) has been proposed as a means to infer causal relations between DNA methylation and disease outcomes [ 14 ]. (biomedcentral.com)
  • For example, inbred mice carrying the agouti viable yellow (A vy ) allele show a range of coat colours associated with different DNA methylation states at the locus. (researchgate.net)
  • Using whole genome bisulphite sequencing of DNA from the livers of five mice from the A vy colony, we searched for sites at which DNA methylation differed among the mice. (researchgate.net)
Routine2
  • 1991) Prime % for Listeria bothering of support interests and drawing of routine techniques. (ligaya-technologies.com)
  • 1998. Routine prenatal cystic fibrosis screening in primary care offices. (cdc.gov)
Successfully1
  • We have successfully applied high-resolution DNA melting (HRM) analysis to distinguish all four haplotypes of the Cntg10192 marker in a single analysis. (biomedcentral.com)
Generate1
  • Using this technique, it is possible to generate human egg cells for IVF that have the genetic material from the intended mother without the defects in the cytoplasm that may be responsible for infertility. (elifesciences.org)
Sequences2
  • In turn, variants in DNA sequences may result in differences in amino acid sequences in the protein coded for by the locus. (scialert.net)
  • Golden Promise were not expressed in the studied barley cultivar Barke although showed primer specificity with their cloned DNA sequences. (biomedcentral.com)
Genome2
  • The availability of next-generation sequencing, in particular whole genome sequencing of bisulphite converted DNA, allows us, for the first time, to search for metastable epialleles at base pair resolution. (researchgate.net)
  • Fully haplotype-resolved genome assembly and DNA proximity analysis revealed that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt , with no recombination or reassortment. (biorxiv.org)
Species1
  • The average protein content of cereals indicates that relatively small differences exist within and between species and that these can be amplified by environmental factors. (biomedcentral.com)
Markers1
  • The markers used in this study created a number of polymorphic bands among the different cultivars that can be utilized as molecular markers for their differentiation. (springeropen.com)
Clinical1
  • This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies. (elifesciences.org)
Pathways2
  • Here, we provide the first evidence of DNA damage associated with the development of imposex (the masculinization of female gastropods considered to be the result of alterations to endocrine-mediated pathways) in the dog-whelk Nucella lapillus . (nih.gov)
  • A strong association was found between TBT body burden and the prevalence of abnormal growths, thereby providing compelling evidence to support the hypothesis that environmental chemicals that affect reproductive processes do so partly through DNA damage pathways. (nih.gov)
Analysis1
  • anything songs are: Statistics distribution and frequency, way, valid Listeria, operator of random groups, variety ampelography, activity and poverty, palmitoylation of fifth and central :RecDescent, and analysis of event and first awardsRelated issues with not database jobs. (ligaya-technologies.com)
Reproductive1
  • Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. (elifesciences.org)
Gene expression1
  • Quantitative RT-PCR with SYBR Green labelling can be a useful technique to follow gene expression levels of large gene families with highly homologues members. (biomedcentral.com)
Mice1
  • applied MST to infertile female mice and found that the technique could permanently correct deficiencies in the cytoplasms of poor quality egg cells, allowing the mice to give birth to healthy offspring. (elifesciences.org)
Identification1
  • The obtained data indicated that SCoT and SDS-PAGE techniques could be used efficiently for identification and differentiation of the selected genotypes. (springeropen.com)
Human1
  • These findings open up the possibility of developing new treatments for infertility caused by problems with egg cells, so experiments involving human egg cells are now being performed to evaluate the safety and effectiveness of the technique. (elifesciences.org)
Positive1
  • In conclusion, the results indicate that incentive programs can have an overall positive impact on both the percentage of FIT kits returned and the number of individuals screened. (cdc.gov)
Time1
  • phite converted DNA, allows us, for the first time, to search f or metastable epialleles at base pair resolution. (researchgate.net)
Average2
  • Over two and a half years data were regularly collected on each shark s length, weight, average consumption, and the percentage body weight consumed. (elasmollet.org)
  • Most Neighborhood Healthcare health centers experienced an increase in the percentage of FIT kit returns (average of 3.6 percentage points) and individuals screened (an average increase of 111 FIT kits per month) from the baseline period through the implementation period. (cdc.gov)