• cell-penetrat
  • Cell-penetrating peptides are a class of carrier molecules that are able to induce the cellular membrane into taking them and their cargo molecules into the cells. (diva-portal.org)
  • Understanding how and why cell-penetrating peptides work is one of the first and most important steps towards improving them to the point where they become useful as carriers for oligonucleotide-based therapies. (diva-portal.org)
  • This thesis is comprised of four scientific papers that are steps toward finding an uptake mechanism for cell-penetrating peptides that have been non-covalently complexed with oligonucleotides. (diva-portal.org)
  • Paper II expands upon this first finding and shows that the same receptors are key players in the uptake of several other cell-penetrating peptides that have been complexed with either, long double-stranded plasmid DNA or short double-stranded RNA. (diva-portal.org)
  • The ability of cell-penetrating peptides to cross plasma membranes has been explored for various applications, including the delivery of bioactive molecules to inhibit disease-causing cellular processes. (diva-portal.org)
  • The uptake mechanisms by which cell-penetrating peptides enter cells depend on the conditions, such as the cell line the concentration and the temperature. (diva-portal.org)
  • Our group has developed a series of highly performing, non-toxic cell-penetrating peptides, all derived from the original sequence of transportan 10. (diva-portal.org)
  • novel cell-penetra
  • To be used as therapeutics, each novel cell-penetrating peptide needs to be fully characterized, including their physicochemical properties, their biological activity and their uptake mechanism. (diva-portal.org)
  • nucleic
  • Can pseudocomplementary peptide nucleic acid nucleases (pcPNANs) be a new tool for genetic engineering? (peerj.com)
  • genetic engineering , pseudocomplementary peptide nucleic acid nucleases , DNA double strand break , homologous recombination , error-prone nonhomologous end joining. (peerj.com)
  • Shi P. ( 2014 ) Can pseudocomplementary peptide nucleic acid nucleases (pcPNANs) be a new tool for genetic engineering? (peerj.com)
  • In this paper, we propose a new tool for genetic engineering, the pseudocomplementary peptide nucleic acid nucleases (pcPNANs), which are composed of a pseudocomplementary PNA (pcPNA) specific for a DNA target sequence, a FokI nuclease cleavage domain and a nuclear localization signal. (peerj.com)
  • These findings extend the knowledge of the variables that condition intracellular Cell-penetrating peptide mediated transport of nucleic acids, which ultimately translates into a small step towards successful non-viral gene therapy. (diva-portal.org)
  • Binding
  • 1. Binding sites for calcitonin-gene-related peptide were localized and characterized in porcine coronary arteries using quantitative autoradiography, and the density of binding sites was compared between large epicardial and small intramyocardial coronary arteries. (clinsci.org)
  • 2. A single class of binding sites for calcitonin-gene-related peptide with a dissociation constant of 2.1 ± 0.2 nmol/l was detected in both the large and small coronary arteries. (clinsci.org)
  • β-Human calcitonin-gene-related peptide showed higher affinity than α-human calcitonin-gene-related peptide for the binding sites. (clinsci.org)
  • Most of the specific binding sites for both peptides in the large coronary artery were localized in the intima and media. (clinsci.org)
  • plasma
  • Background- The diagnostic and prognostic values of plasma B-type natriuretic peptide (BNP) testing are established. (ahajournals.org)
  • Plasma B-type natriuretic peptide (BNP) measurement has been established as a helpful aid in the diagnosis of heart failure (HF). (ahajournals.org)
  • Increased plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) concentration reflects cardiac overload and is used to diagnose and stage congestive heart failure. (ahajournals.org)
  • cells
  • The general purpose of this thesis is to reveal the mechanisms by which our in house designed peptides enter cells and allow the successful transport of biofunctional oligonucleotide cargo. (diva-portal.org)