• Sets of overlapping peptides from potential target vaccine proteins are screened with immune sera or cells to find vaccine or diagnostic test candidates. (mimotopes.com)
  • Peptides mimicking protein activity can overcome the perceived limitations of therapeutic proteins. (ox.ac.uk)
  • Linear epitope mapping in the E and NS1 proteins of dengue and Zika viruses: Prospection of peptides for vaccines and diagnostics. (bvsalud.org)
  • The serum samples from dengue and Zika patients detected epitopes evenly distributed across the viral proteins in a peptide microarray platform. (bvsalud.org)
  • The serum samples from dengue and Zika patients showed a high level of cross-reactivity with peptides in the DENV and ZIKV proteins . (bvsalud.org)
  • Additionally, these reactions simultaneously react on several post-translationally modified groups in peptides or proteins. (nih.gov)
  • The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
  • The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases. (bvsalud.org)
  • These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. (blockingantibody.com)
  • The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs. (blockingantibody.com)
  • AnaSpec offers 320 reagents for peptide synthesis. (anaspec.com)
  • Peptides represent over decades of innovative peptide synthesis expertise. (anaspec.com)
  • Oligomers of Aβ peptide are implicated as the most probable causative agent in Alzheimer's disease. (nature.com)
  • Rodda, S.J. (2002) Peptide Libraries for T-Cell Epitope Screening and Characterization. (mimotopes.com)
  • Peptide mapping, a bottom-up approach to protein characterization, enables the profiling of a protein's primary structure as well as its post-translational modifications (PTMs) such as oxidation, deamidation, and glycosylation. (sigmaaldrich.com)
  • The use of a high-resolution mass spectrometry-based approach for the characterization is commonly referred to as a multi-attribute method (MAM) 1 , as the peptide mapping analysis yields multiple pieces of information about the protein in a single analysis. (sigmaaldrich.com)
  • Identification and characterization of the chemokine-binding interface of evasins could thus inspire the development of novel anti-inflammatory peptides that therapeutically target the chemokine network in inflammatory diseases. (ox.ac.uk)
  • The chemical reactions on these modifications change the peptide mapping pattern, and information from these reactions may contribute characterization and location of post-translational modified groups in the protein. (nih.gov)
  • Our expert team brings decades of experience in protein and peptide characterization to provide the right data with the right insight at all stages of development. (kbibiopharma.com)
  • Here, we provide an optimized method for tryptic digestion and peptide mapping using adalimumab as an example. (sigmaaldrich.com)
  • iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale. (kent.ac.uk)
  • An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP). (sigmaaldrich.com)
  • SF samples from end-stage knee osteoarthritis patients undergoing total knee replacement surgery and controls, that is, deceased donors without known knee disease were previously run using liquid chromatography mass spectrometry (LC-MS). This data was used to perform new database searches generating results for non-tryptic and semi-tryptic peptides for studies of degradomics in OA. (lu.se)
  • Check out out latest paper Exploring the Sequence Space of Antimicrobial Peptide Dendrimers in Isreal Journal of Chemistry! (unibe.ch)
  • Check out our latest paper Antimicrobial Peptide-Peptoid Hybrids with and without Membrane Disruption in ACS Infectious Diseases! (unibe.ch)
  • The set consisted of short overlapping peptides derived from the protein being investigated and the peptides were captured onto plate wells coated with streptavidin before completing an ELISA with each monoclonal antibody (Figure 1). (mimotopes.com)
  • Following digestion, peptides and glycopeptides are separated and analyzed by RP-LC-MS.M. (sigmaaldrich.com)
  • The filter membrane retains the mAb of interest until the digestion procedure is complete, following which peptides can pass through the filter while excluding trypsin. (sigmaaldrich.com)
  • With over 25 years of peptide manufacturing experience, AnaSpec is your trusted source of highly complex peptides. (anaspec.com)
  • AnaSpec manufactures many categories of peptides in Fremont, California, USA. (anaspec.com)
  • Morris, H.R. et al: Isolation and Characterisation of Human Calcitonin Gene-Related Peptide (CGRP). (biopharmaspec.com)
  • The effect of human alpha-calcitonin gene-related peptide (CGRP) and sodium nitroprusside have been measured on human isolated mesenteric vasculature and on rings of human superior mesenteric artery and saphenous vein. (biopharmaspec.com)
  • Linear antibody epitopes are readily mapped with PepSets. (mimotopes.com)
  • T-cell epitopes (Helper and Cytotoxic) are easily located using PepSets provided peptides of the correct length are used2. (mimotopes.com)
  • However, several epitopes were located within " epitope hotspots", characterized by clusters of peptides recognized in more than 30% of the sub-arrays analyzed using individual or pooled serum samples. (bvsalud.org)
  • In addition motif redundancy can affect quantitative and statistical analysis and prevent a realistic comparison of peptide numbers between datasets. (biorxiv.org)
  • Binding Mechanisms of Amyloid-like Peptides to Lipid Bilayers and Effects of Divalent Cations. (acs.org)
  • The structure of the oligomer with the S/T-rich region is a large step forward in the understanding of the function of DNAJB6 and how it can bind aggregation-prone peptides and prevent amyloid diseases. (lu.se)
  • While the aggregation of the amyloid β peptide (Aβ) into amyloid fibrils is highly implicated as a key factor in the disease, the molecular nature of its involvement has proven complex and elusive. (lu.se)
  • Control peptides were used to further characterize the chirality of the final nickel-peptide complex, and DFT calculations were performed to validate the hypothesized position of the chiral inversions. (ku.edu)
  • When a bioactive sequence is already known, analoging studies can be carried out using the set of peptides comprising single-point substitution sequences, or a combination of 2 or more selected substitutions. (mimotopes.com)
  • Sequences exhibiting enhanced bioactivity, or inhibitory properties, are candidates for development as peptide or peptidomimetic drugs. (mimotopes.com)
  • The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
  • The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
  • The metal abstraction peptide (MAP) tag is a tripeptide sequence capable of abstracting a metal ion from a chelator and binding it with extremely high affinity at neutral pH. (ku.edu)
  • Antibody epitope mapping by ELISA is simple to perform using biotinylated peptides because the peptides can be captured onto Streptavidin™ or NeutrAvidin™ coated plates through the strong noncovalent avidin-biotin binding. (mimotopes.com)
  • Custom synthesized biotinylated PepSets for linear antibody mapping are designed by the research scientist with help from experienced Technical Consultants at Mimotopes, and are shipped either ready to redissolve and plate or as ready-to-use plates already coated with the peptide (Figure 2). (mimotopes.com)
  • This application is summarized in a separate article entitled Truncated Peptide Libraries for Cytotoxic T-Cell Epitope Mapping, available on the Mimotopes website. (mimotopes.com)
  • In addition, two DENV peptides detected at similarly high rates by both dengue and Zika patients were also found within the epitope hotspots. (bvsalud.org)
  • Thus, epitope hotspots may have greater potential to serve as antigens in diagnostic tests and vaccine development than peptides composed of only 15 amino acids . (bvsalud.org)
  • This approach first identifies and locates a product-related impurity (a critical quality attribute) caused by isomerization, deamidation, oxidation, or other post-translational modifications, and then utilizes synthetic peptides and MS to assist the development of a LC-UV-based chromatographic method that separates and quantifies the product-related impurities by UV peaks. (pda.org)
  • till polypeptides that are long, continuous, and unbranched synthetic peptide chains. (blockingantibody.com)
  • Abstract Among synthetic analogues of antimicrobial peptides (AMPs) under investigation to address antimicrobial resistance, peptoids (N-alkylated oligoglycines) have been reported to act both by membrane disruption and on intracellular targets. (unibe.ch)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • In this application note, we profile capsid protein peptides from AAV5 and AAV9, digested with trypsin using a Biozen™ Peptide XB-C18 reversed phase column. (phenomenex.com)
  • A highly specific protease, trypsin, is used to cleave the protein into suitably sized peptides, although a combination of multiple proteases may also be used to generate more complete PTM site information. (sigmaaldrich.com)
  • The major phosphopeptides derived from biosynthetically labeled c-Fos correspond to phosphopeptides generated after phosphorylation of c-Fos in vitro with both RSK and MAP kinase. (nih.gov)
  • Cooperative phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide phosphorylated on both sites, as opposed to singly phosphorylated peptides. (nih.gov)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • This thesis and the work herein is part of an ongoing effort to map out the aggregation mechanism of Aβ in vitro in as much detail as possible, in the hope to provide a better basis for understanding its role in disease. (lu.se)
  • We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
  • The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
  • This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
  • Known proteolytic events (from the MEROPS peptidase database) were mapped to the dataset, allowing the identification of potential proteases and which substrates they cleave. (lu.se)
  • Structure-Guided Chemical Optimization of Bicyclic Peptide (Bicycle) Inhibitors of Angiotensin-Converting Enzyme 2. (acs.org)
  • To determine the suitability of an instrument for RPLC-MS analysis, a mixture of 14 isotopically labeled peptides is analyzed as a system suitability test prior to each run. (sigmaaldrich.com)
  • However, motif redundancy among detected peptides by mass spectrometry (MS) leads to errors and ambiguities in the analysis. (biorxiv.org)
  • In this work, we utilized a mass spectrometry-based peptide mapping approach to detect and quantitate the Asp isomerization in the CDRs of mAb-A. To routinely monitor the CDR isomerization of mAb-A, a focused peptide mapping method utilizing reversed phase chromatographic separation and UV detection has been developed and qualified. (pda.org)
  • We used hydrogen-deuterium exchange mass spectrometry to map the binding interface of the evasin P672 that physically interacts with C-C motif chemokine ligand 8 (CCL8) and synthesized a 16-mer peptide (BK1.1) based on this interface region in evasin P672. (ox.ac.uk)
  • The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)
  • Lysine-specific crosslinking mass spectrometry provided distance constraints to select a homology model of the DNAJB6 monomer, which was subsequently used in crosslink-assisted docking to generate a dimer model, revealing that a peptide-binding cleft lined with S/T-residues is formed at the monomer-monomer interface. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • One approach to achieving a non-redundant quantitation of phospho-motifs is to quantify each peptide but only assign a reference peptide, according to some quality criteria, as the quantification of the motif. (biorxiv.org)
  • Three chemical specific cleavage reactions, one for the carboxyl side of aspartyl peptide bonds, one for the carboxyl side of asparaginyl peptide bonds and another for the amino side of seryl/threonyl peptide bonds have been recently established. (nih.gov)
  • The 2H relaxation data also provided information about the orientational order and internal motion of OD and ND bonds in side-chains and surface peptides. (lu.se)
  • PepSets have several advantages over other screening methods such as noncleaved peptides and blots. (mimotopes.com)
  • One person had strong humoral and weak cellular immune reactivity to SIVcol peptides. (cdc.gov)
  • Development of Potent and Selective Phosphinic Peptide Inhibitors of Angiotensin-Converting Enzyme 2. (acs.org)
  • 1 of a panel of 9 SIV IDR MAPs ( Figure 1 ), 17.1% were seroreactive in the high exposure group, 7.8% in the low exposure group, and 2.3% in the general group. (cdc.gov)
  • A news article on the groups recent paper Chemical space guided discovery of antimicrobial bridged bicyclic peptides against Pseudomonas aeruginosa and its biofilms has been published by the monthly chemistry news magazine Chemistry World, which in turn is published by the Royal Society of Chemistry. (unibe.ch)
  • We used linear mixed models to estimate differences in peptide-level expression between the two groups. (lu.se)
  • This pD dependence is due to labile protein deuterons in acidic side-chains and surface peptide groups, which exchange rapidly with water deuterons. (lu.se)
  • Here we show that peptides possessing multiple-chemokine-binding and anti-inflammatory activities can be developed from the chemokine-binding site of an evasin. (ox.ac.uk)
  • The SMAP-EIA also detects other SIV strains not represented by specific SIV lineage-based peptides. (cdc.gov)
  • Accurate quantification and enumeration of peptide motifs is hampered by redundancy in peptide identification. (biorxiv.org)