Peptides proteins. Medical search. Frequent questions

Providing a comprehensive review of bioactive proteins and peptides obtained from food sources, the book brings together the most up-to-date and essential information from eminent researchers from all over the world. (routledge.com)
Choose from hundreds of bioactive proteins for functional assays including cell proliferation assays, enzyme assays, or functional ELISAs. (novusbio.com)

The Robetta Server requires a minimum AA length of 26 as the various tools used during fragment generation like secondary structure prediction are not optimized for very small peptides. (rosettacommons.org)
On the basis of work on small peptides, it is possible to assign all the downfield resonances in denatured proteins to single types of carbon atoms, with the exception of the carbonyl carbons and the histidine Cγ resonance. (monash.edu)
Therefore, we assayed the inhibitory effects of small peptides derived from S protein on the binding of S protein to ACE2 and on the S-protein-pseudotyped retrovirus infectivity. (scienceopen.com)
In conclusion, this is the first report of small peptides designed to disrupt the binding of SARS-CoV S protein to ACE2. (scienceopen.com)

A number of natural antimicrobial peptides are cyclic, and the products of SICLOPPS are "increasingly viewed as ideal backbones for modulation of protein-protein interactions. (wikipedia.org)
Using SICLOPPS for the discovery of novel antimicrobial peptides and their targets" (PDF). (wikipedia.org)
A putative Vibrio cholerae two-component system controls a conserved periplasmic protein in response to the antimicrobial peptide polymyxin B. (broadinstitute.org)
One stress that V. cholerae encounters in the host is exposure to antimicrobial peptides on mucosal surfaces. (broadinstitute.org)
We used massively parallel cDNA sequencing (RNA-Seq) to quantitatively identify the transcriptome of V. cholerae grown in the presence and absence of sub-lethal concentrations of the antimicrobial peptide polymyxin B. We evaluated the transcriptome of both wild type V. cholerae and a mutant carrying a deletion of vc1639, a putative sensor kinase of an uncharacterized two-component system, under these conditions. (broadinstitute.org)
In order to identify by a rational approach further human proteins carrying cryptic HDPs, we recently developed an in-silico screening method to localize antimicrobial regions hidden inside the primary structure of precursor proteins. (unina.it)
A wide list of potential new antimicrobial peptides was obtained by applying this method to about 4,000 human extracellular proteins. (unina.it)
We demonstrated that both peptides are endowed with a significant antimicrobial activity towards Gram-negative and Gram-positive strains, and are able to prevent biofilm formation in several strains at concentrations lower than those required to directly kill planktonic bacterial cells. (unina.it)
Antimicrobial protein candidates from the thermophilic Geobacillus sp. (lu.se)

In a subsequent study, collagen peptide sequences from an approximately 78 Myr Brachylophosaurus canadensis fossil were reported which has remained largely unchallenged. (figshare.com)
However, the endogeneity of these sequences relies heavily on a single peptide sequence, apparently unique to both dinosaurs. (figshare.com)
Regardless of the true nature of the dinosaur peptides, our finding highlights the difficulty of differentiating such sequences with confidence. (figshare.com)
One of the peptide sequences used is within the beta-amyloid fragment and recognizes the extracellular amyloid plaques observed in Alzheimer patients' brains (see Cat. (antibodiesinc.com)
Three other peptide sequences used, however, are within the extracellular domain of APP outside of this domain, and are useful for identifying the APP protein itself, and various proteolytic fragments, rather than the beta-amyloid peptide and plaques. (antibodiesinc.com)
Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
Peptide libraries are established after the identification of peptide sequences. (bvsalud.org)

The members of the group are experts in food microbiology , microbial biotechnology of filamentous fungi and yeasts , and in the characterization of bioactive peptides and proteins . (csic.es)
Once optimized the production system, a broad characterization of two novel recombinant peptides, previously identified in human Apolipoprotein B (ApoB), was performed. (unina.it)
Additional fragmentation analysis and charge deconvolution tools also enhance its use for protein characterization. (advion.com)

Whole protein and defined CD8(+) and CD4(+) peptides linked to penetratin targets both MHC class I a. (edu.au)
Although modern SPPS shows a high degree of automation, some SPPS peptide targets remain challenging to synthesize and a single suboptimal synthesis cycle can cause costly re-runs in large scale or long chain protein production - rapid analysis of each reaction step can prevent this. (advion.com)
Defects in these networks result in a variety of disease states making protein kinases important targets for drug design [ 3 ]. (biomedcentral.com)

It specifically cleaves before lysine and arginine residues in proteins. (sigmaaldrich.com)
This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra. (sigmaaldrich.com)
SP-4 (residues 192-203), SP-8 (residues 483-494), and SP-10 (residues 668-679) significantly blocked the interaction between S protein and ACE2 by biotinylated enzyme-linked immunosorbent assay, with IC 50 values of 4.30 ± 2.18, 6.99 ± 0.71, and 1.88 ± 0.52 nmol, respectively. (scienceopen.com)
Peptide scanning suggested the region spanning residues 660-683 might act as a receptor-binding domain. (scienceopen.com)
This antibody recognizes the peptide EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the human APP gene product. (antibodiesinc.com)
Chickens were immunized with a keyhole limpet hemocyanin (KLH) conjugate of the synthetic peptide EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the human gene product (NP_000475.1). (antibodiesinc.com)
New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)
The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
2. Methods and Materials 2.1 Components The DENV2 NS2B-NS3pro expression plasmid encoding the protease precursor found in this research provides the hydrophilic domains of NS2B cofactor (48 proteins) as well as the NS3pro domains (185 residues) (Yon et al. (findadig.com)

When administered by conventional delivery methods, such as injection, these compounds are eliminated rapidly, resulting in wide variations in the level of recombinant protein in plasma and tissues. (alzet.com)
Since the approval of the first protein / peptide-based therapy ( recombinant human insulin ) in 1982, there has been a substantial increase in the R&D initiatives focused on such products. (rootsanalysis.com)

Interestingly, novel functions of these peptides are still being described to date. (unina.it)
Interestingly, in the last decade, three orally administered protein / peptide-based therapies have been approved, namely Mycapssa® (2020), Trulance® (2017) and Linzess® (2012) across different geographies. (rootsanalysis.com)
Recently we have proposed proteolytic fragment peptides as an additional source of bioactivity, which interestingly were significantly associated with exosomal proteins after enrichment analysis. (cdc.gov)

Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. (curehunter.com)
Artificial proteins and peptides represent a powerful and versatile class of molecules. (anl.gov)

Dear Netters, We are interested in purifying a protein (from detergent extracted microsome preps) which binds a particular peptide. (bio.net)
Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. (rcsb.org)
An artificially designed symmetric peptide macrocycle which binds a metal ion at its center, and which acts as a metal-triggered conformational switch. (anl.gov)

Peptide therapeutics can also be highly personalized, allowing RCT to design each treatment to a patient's specific needs. (prnewswire.com)
Although there is much debate over the mechanism by which this "protein transduction" occurs, the ability of CPPs to translocate rapidly into cells is being exploited to deliver a broad range of therapeutics including proteins, DNA, antibodies, oligonucleotides, imaging agents and liposomes in a variety of situations and biological systems. (benthamscience.com)
The rising popularity of this upcoming class of therapeutics has led to the reformulation / development of oral protein / peptide-based product candidates for the treatment of a myriad of disease indications across multiple therapeutic areas ( including but not limited to metabolic disorders, gastrointestinal disorders, autoimmune disorders ). (rootsanalysis.com)
Protein / peptide-based therapeutics have revolutionized the pharmaceutical landscape, this can be attributed to the wide applications and target specific nature of such interventions. (rootsanalysis.com)

Circular peptides tend to be resistant to protease activity, and may be suitable for use as orally administered drugs. (wikipedia.org)

Amyloid Precursor Protein (APP, UniProt Accession Number P05067) is a 770 amino acid, single-pass transmembrane protein whose beta-amyloid proteolytic fragment can form neurotoxic extracellular accumulations in human cerebral cortex, and is widely believed to be the cause of Alzheimer's dementia. (antibodiesinc.com)

With PEPTIPLUS ® , GELITA offers pure collagen proteins which can be used in a variety of functional sport bars, beverages or as protein powder. (nutraingredients.com)
Collagen peptides are made by breaking down whole collagen proteins into smaller pieces. (medlineplus.gov)

Identification of Novel Dipeptidyl Peptidase-IV Inhibitory Peptides in Chickpea Protein Hydrolysates. (bvsalud.org)
Some food protein -derived peptides are thought to have DPP-IV inhibitory (DPP-IVi) activity. (bvsalud.org)
A family of inhibitory proteins which bind to the REL PROTO-ONCOGENE PROTEINS and modulate their activity. (bvsalud.org)

Split-intein circular ligation of peptides and proteins (SICLOPPS) is a biotechnology technique that permits the creation of cyclic peptides. (wikipedia.org)
ALZET pumps have been used extensively to study the effects of proteins and peptides and other biotechnology compounds in vivo . (alzet.com)

Neuronal Calcium Sensor Protein, otherwise referred to as NCS, is a substantial protein family that operates as calcium-dependent molecular switches. (prospecbio.com)
This fact demonstrates that the His-mediated, proton sponge-based endosomal escape saturates at moderate amounts of internalized protein, a fact that might be critical for the design of protein materials for cytosolic molecular delivery. (upc.edu)
Molecular docking verified that the four screened peptides (AAWPGHPEF, LAFP, IAIPPGIPYW, and PPGIPYW) could bind to the active center of DPP-IV. (bvsalud.org)

A synthetic peptide containing the HAV-specific amino acid sequence of one of these sites induced anti-HAV-neutralizing antibodies. (scienceopen.com)
This peptide sequence is also 100% conserved between human, mouse and rat homologs of APP, and the antibodies against this sequence cross-react with all three species. (antibodiesinc.com)

These peptides are produced by ribosomal protein synthesis, followed by an intein-like event that splices the protein into a loop. (wikipedia.org)
The new platform improves the workflow of chemists performing peptide synthesis and needing fast mass analysis of each reaction step as well as final products. (advion.com)
Solid phase peptide synthesis (SPPS) allows for rapid, parallel synthesis of peptides of various amino acid (AA) lengths, some of which routinely exceed 30 or 40 in length. (advion.com)
We don't just give you insights into our peptide synthesis systems, inform about our latest innovations and products, or provide an insider view into our company. (jpt.com)

In order to determine the specificity I perpared a 6 mM solution of the peptide in binding buffer (MES 100 mM 1 % CHAPS detergent) after binding the protein and washing the column I added 2 ml of the elution buffer (6 mM peptide in wash buffer) and the column (1 ml) swelled up to 5 ml and would not settle. (bio.net)
Find blocking peptides and proteins for use as quality controls to confirm antibody specificity. (novusbio.com)
We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
The relative contribution of substrate recruitment and peptide specificity to protein kinase substrate specificity varies between protein kinases. (biomedcentral.com)
The prediction of peptide specificity is therefore the basis for most of the available computational methods aimed at predicting substrates of protein kinases. (biomedcentral.com)

Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors. (usda.gov)

Small Molecule Amyloid-β Protein Precursor Processing Modulators Lower Amyloid-β Peptide Levels via cKit Signaling. (j-alz.com)
Bulk Order Inquiry for Anti-Amyloid Precursor Protein, Peptide #5 Antibody ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)
RNA genome can be translated to an individual polyprotein precursor which can be processed to create 10 adult proteins by co- and post-translational digesting (Rice and Lindenbach, 2003). (findadig.com)

The viral protease cleavage sites have in common a set of basic proteins, K and R, accompanied by G, S, or A on the P1 placement (Lindenbach and Grain, 2003). (findadig.com)
1993). Using an protease assay using fluorogenic peptide substrates was set up (Yusof et al. (findadig.com)
Subsequent proteomic mass spectrometry revealed that MWCNT treatment significantly altered serum exosome protein cargo, with particular increases in focal adhesion proteins and decreases in protease inhibitors, changes consistent with altered endocytosis and signaling. (cdc.gov)

The expression of metal-binding proteins or peptides in microorganisms and plants in order to enhance heavy metal accumulation and/or tolerance has great potential. (lu.se)

Though it is seen that the fragment generated using the same protocol shows good coverage of peptide conformational space and thus can be used. (rosettacommons.org)
I'm having the same question that how to generate fragment library for peptide(9 length).Could you provide me a detailed tutorial on FlexPepDock ab-initio( [email protected] )? (rosettacommons.org)
Thanks!I know how to generate a fragment file for peptide(6-15 length)now. (rosettacommons.org)

This assembly results in a spontaneous, essentially traceless splicing reaction that covalently links the two flanking protein segments through native chemical ligation 9 . (biorxiv.org)

Cytoplasmic delivery and cross-presentation of proteins and peptides is necessary for processing and presentation of antigens for the generation of cytotoxic T cells. (edu.au)

Many human proteins with functions not necessarily related to host defense behave as sources of HDPs. (unina.it)
Protein kinases, the enzymes responsible for protein phosphorylation, make up almost 2% of protein-encoding genes in the human genome [ 1 ] and an estimated 30-50% of human proteins are phosphorylated [ 2 ]. (biomedcentral.com)

Neuronal Calcium Sensor Proteins can regulate signal transduction while showing exceptional conservation of yeast to humans. (prospecbio.com)
Included here are peptides and proteins that regulate the activity of transcription factors and cellular processes in response to signals from cell surface receptors. (usda.gov)
Protein kinases and their substrates regulate essentially all cellular processes through complex regulatory networks, in which phosphorylated proteins act as switches that tune the response of the cell to environmental stimuli. (biomedcentral.com)

Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)

Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. (scienceopen.com)
To better understand these functions, we have made a set of five anti-peptide antibody reagents against APP. (antibodiesinc.com)
This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat. (antibodiesinc.com)

Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells. (umassmed.edu)

However, with the technological advancements in delivery formulations, oral delivery of therapeutic interventions has gained significant traction, prompting stakeholders to leverage their expertise in the development of orally administrable proteins / peptides. (rootsanalysis.com)
However, oral formulations of protein / peptide-based drugs have been associated with several drawbacks, including poor bioavailability and drug decomposition in the gastrointestinal tract. (rootsanalysis.com)

Manipulation of proteins by chemical modification is a powerful way to decipher their function or harness that function for therapeutic purposes. (biorxiv.org)
VisP is a conserved periplasmic protein implicated in lipid A modification in Salmonellae. (broadinstitute.org)

Oral Ingestion of AP Collagen Peptide Leads to Systemic Absorption of Gly-Pro-Hyp, Alleviating HO-Induced Dermal Fibroblast Aging. (medlineplus.gov)

Proteins and peptides that are involved in signal transduction within the cell. (usda.gov)

He also works on the hydrolysis of waste seafood proteins for bioactive peptide generation and their action as antioxidants and in antidiabetic, anticancer, and high blood pressure regulation. (routledge.com)

They discuss recent developments in proteomics, bioavailability, and opportunities for designing future peptide-based foods. (routledge.com)

The new features significantly enhance the ability of Predikin to analyse protein kinases and their substrates. (biomedcentral.com)
In general, a protein kinase acts on a discrete set of substrates to ensure that signalling fidelity is maintained. (biomedcentral.com)

Several recent developments have taken place in the field of oral peptides and proteins. (rootsanalysis.com)

Find your peptide or protein of interest using the search tool below. (novusbio.com)

Different from traditional drugs, peptides occur naturally in the body. (prnewswire.com)
This project aims to reduce the computational and energetic costs of producing successful peptide macrocycle drugs or industrial enzymes using two approaches. (anl.gov)
Given the ongoing research and development initiatives and an encouraging response to the already marketed drugs, the oral protein / peptide therapies market is poised to witness significant growth over the coming decade. (rootsanalysis.com)

He is the Editor-in-Chief of two Bentham journals, Protein and Peptide Letters, for research reports and mini-reviews, and Current Protein and Peptide Science, which publishes exclusively review papers. (eurekaselect.com)

This project aims to improve the accuracy and decrease the computational cost of computational methods for designing peptides and proteins for medical and manufacturing applications. (anl.gov)

2012) or of WNV NS2B-NS3pro within a covalent complicated using a tetra-peptide substrate-based inhibitor (Erbel et al. (findadig.com)

Next, the contributors present information on therapeutic peptides. (routledge.com)
Protein Transduction: Cell Penetrating Peptides and Their Therapeutic Applications, Frontiers in Medicinal Chemistry (2010) 5: 98. (benthamscience.com)

PUERTO PENASCO, Mexico , Oct. 13, 2015 /PRNewswire/ -- Regenerative Cellular Therapy (RCT), a new clinic in Puerto Penasco, Sonora, Mexico , is changing the course of major diseases like Alzheimer's and arthritis through the transformative power of natural peptide-based therapies. (prnewswire.com)

We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)

Once a cyclic peptide is identified with a biological activity of interest, it may also be possible to identify the target of the peptide (a gene that encodes a protein with which it interacts) by functional complementation, facilitating a better understanding of its mechanism of action. (wikipedia.org)

In this study, we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins, which can specifically bind and be internalized by the target cells through the tumoral marker CXCR4. (upc.edu)

Snake venoms are complex mixtures of protein and peptide toxins, varying from one species to another, and even within species. (who.int)

LysargiNase is suitable for peptide or protein digestion in solution or in gel. (sigmaaldrich.com)
For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended. (sigmaaldrich.com)

The numer- ical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic release. (upc.edu)

Among the tested candidates, the peptide H5E (HEHEHEHEH) is promising as a good promoter of endosomal escape of the associated full- length protein upon endosomal internalization. (upc.edu)

Find functional native proteins isolated from serum, cell, and tissue sources. (novusbio.com)
It interacts also with anticoagulant protein S and with serum amyloid P component. (lu.se)

3) The development of fungi as sustainable and efficient biofactories to produce compounds and proteins of agricultural and food interest. (csic.es)
To develop filamentous fungi from the genera Aspergillus and Penicillium as safe and sustainable biofactories to produce compounds and proteins (including antifungal proteins) of agricultural and food interest (participation in the H2020 European project Pre-HLB). (csic.es)

In this study, chickpea protein hydrolysates (CPHs) obtained through Neutrase hydrolysis for 60 min (CPHs-Pro-60) exhibited the highest DPP-IVi activity. (bvsalud.org)

Anatomy7

Organisms8

Diseases1

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes39

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science3

Health Care1

Bioactive proteins2

Small peptides4

Antimicrobial9

Sequences7

Characterization3

Targets3

Residues12

Recombinant2

Interestingly3

Molecules2

Binds3

Therapeutics4

Orally administered1

Proteolytic fragment1

Collagen proteins2

Inhibitory3

Biotechnology2

Molecular3

Sequence2

Synthesis4

Specificity5

Intracellular2

Precursor3

Protease3

Microorganisms1

Fragment3

Ligation1

Antigens1

Human proteins2

Regulate3

Membrane proteins2

Antibody3

Fluorogenic1

Formulations2

Modification2

Absorption1

Signal1

Antioxidants1

Proteomics1

Substrates2

Recent developments1

Search1

Drugs3

Current Protein1

Methods1

Covalent1

Therapeutic2

Therapies1

Synthetic2

Gene1

Modular1

Mixtures1

Digestion2

Cytoplasmic1

Internalization1

Serum2

Compounds2

Hydrolysis1