• protein therapeutics
  • With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. (advion.com)
  • Reversed phase liquid chromatography (RPLC) of peptides is routinely used in the biopharmaceutical industry to provide information on the nature and quality of protein therapeutics. (thermofisher.com)
  • chromosome
  • These four clones (561, 1830, 1631 and 991 bp), which mapped to chromosome 11p15, were all found to contain degenerate 87-base-pair tandem repeats which encode non-repetitive peptides. (biochemj.org)
  • Genetic mapping of the loci controlling the class I and class II antigens of the mouse showed them to be part of a cluster, which Klein mapped to the chromosome 17 and for which he championed the name Major histocompatibility locus, Mhc. (wikipedia.org)
  • hydrophobic
  • The peptide is composed of alternate hydrophobic and hydrophilic domains which probably differ in the extent to which they are glycosylated. (biochemj.org)
  • However, the activation energy can be lowered (and the isomerization catalyzed) by changes that favor the single-bonded form, such as placing the peptide group in a hydrophobic environment or donating a hydrogen bond to the nitrogen atom of an X-Pro peptide group. (wikipedia.org)
  • Seamless
  • Seamless generation of screen lists from peptide mapping results for easy similarity assessments or quality attributes monitoring across large datasets. (bruker.com)
  • bonds
  • This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. (wikipedia.org)
  • The trans form is preferred overwhelmingly in most peptide bonds (roughly 1000:1 ratio in trans:cis populations). (wikipedia.org)
  • Both of these mechanisms for lowering the activation energy have been observed in peptidyl prolyl isomerases (PPIases), which are naturally occurring enzymes that catalyze the cis-trans isomerization of X-Pro peptide bonds. (wikipedia.org)
  • Nevertheless, peptide bonds can undergo chemical reactions, usually through an attack of an electronegative atom on the carbonyl carbon, breaking the carbonyl double bond and forming a tetrahedral intermediate. (wikipedia.org)
  • retention
  • With rock steady retention times, you can be confident in the identity of every peptide in discovery, development and QC environments. (thermofisher.com)
  • mass
  • One mass spectrometer for peptide mapping and intact characterization. (thermofisher.com)
  • In bio-informatics, a peptide-mass fingerprint or peptide-mass map is a mass spectrum of a mixture of peptides that comes from a digested protein being analyzed. (wikipedia.org)
  • Once formed, a peptide-mass fingerprint can be used to search in databases for related protein or even genomic sequences, making it a powerful tool for annotation of protein-coding genes. (wikipedia.org)
  • One major advantage to mass fingerprinting is that it is significantly faster to carry out than peptide sequencing, yet the results are equally useful. (wikipedia.org)
  • Because of this sensitivity, sample preparation is likely the most important step in forming a peptide-mass fingerprint. (wikipedia.org)
  • The use of a peptide-mass fingerprint is fairly widespread in proteomic research. (wikipedia.org)
  • consists
  • Cupiennin 1 family is the more well-known group, and consists of at least four peptides, such as cupiennin 1a, 1b, 1c, and 1d. (wikipedia.org)
  • results
  • Even then, if reliable results are obtained, there must be a matching peptide sequence in the database you are searching in order for the results to be useful. (wikipedia.org)
  • identify
  • The new AdvanceBio Peptide Mapping column can rapidly determine the primary protein structure and identify modifications with unparalleled speed and sensitivity. (technologynetworks.com)
  • Here, we identify the metal abstraction peptide (MAP) as an inhibitor of MMP-8 and demonstrate that tethering MAP to methacrylate polymers effectively inhibits catalysis. (ku.edu)
  • In biological applications, benzophenones have been used extensively as photophysical probes to identify and map peptide-protein interactions. (wikipedia.org)
  • significantly
  • A nonnative isomer of some peptide groups can disrupt the conformational folding significantly, either slowing it or preventing it from even occurring until the native isomer is reached. (wikipedia.org)
  • Earlier
  • Earlier, Hugh O. McDevitt and his coworkers mapped an Immune response-1 (Ir-1) locus influencing the level of antibody production against the synthetic polypeptide (T,G)-L-A into the Mhc. (wikipedia.org)
  • In fact, members of this family were discovered earlier in 1994 as member of the CSTX peptides. (wikipedia.org)
  • groups
  • However, X-Pro peptide groups tend to have a roughly 30:1 ratio, presumably because the symmetry between the C α {\displaystyle \mathrm {C^{\alpha }} } and C δ {\displaystyle \mathrm {C^{\delta }} } atoms of proline makes the cis and trans isomers nearly equal in energy (See figure, below). (wikipedia.org)
  • nonnative isomers of other peptide groups may not affect folding at all. (wikipedia.org)
  • Popular
  • He also developed and published a peptide fingerprinting technique that was so popular that it became a citation classic Cleveland did postdoctoral work with William J. Rutter at the University of California at San Francisco from 1978 to 1981. (wikipedia.org)
  • matrix
  • The energy transfer is sufficient to promote the ionisation and transition of matrix molecules and peptides from the solid phase into the gas phase. (wikipedia.org)
  • similar
  • TGFβR1 (ALK5) and TGFβR2 have similar ligand-binding affinities and can be distinguished from each other only by peptide mapping. (wikipedia.org)
  • Due to its resonance stabilization, the peptide bond is relatively unreactive under physiological conditions, even less than similar compounds such as esters. (wikipedia.org)
  • However
  • However the AppliedBiosystems MAP substrate for peptide synthesis has been suggested, but not by anyone with much experience using it. (bio.net)