• HPLC
  • In fact, it can produce exceptional analytical results two to three times faster than conventional peptide mapping with fully porous HPLC columns, which can take up to 60 minutes. (technologynetworks.com)
  • The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™ PEPTIDE core-shell HPLC/UHPLC columns and determine the feasibility of transferring a method developed on UV to MS simply by adjusting the acidic modifier in the mobile phase. (advion.com)
  • sequences
  • Central to this process is the idea that small peptide libraries contain sequences that will bind to independent regions on a protein surface, and that these ligands can be combined on synthetic scaffolds to create high affinity bivalent reagents. (pubmedcentralcanada.ca)
  • To demonstrate the feasibility of this approach, an array of 4,000 unique 12-mer peptides was screened to identify sequences that bind to non-overlapping sites on the yeast regulatory protein Gal80. (pubmedcentralcanada.ca)
  • The peptide masses are compared to either a database containing known protein sequences or even the genome. (wikipedia.org)
  • Once formed, a peptide-mass fingerprint can be used to search in databases for related protein or even genomic sequences, making it a powerful tool for annotation of protein-coding genes. (wikipedia.org)
  • hydrophobic
  • The peptide is composed of alternate hydrophobic and hydrophilic domains which probably differ in the extent to which they are glycosylated. (biochemj.org)
  • However, the activation energy can be lowered (and the isomerization catalyzed) by changes that favor the single-bonded form, such as placing the peptide group in a hydrophobic environment or donating a hydrogen bond to the nitrogen atom of an X-Pro peptide group. (wikipedia.org)
  • bonds
  • This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. (wikipedia.org)
  • The trans form is preferred overwhelmingly in most peptide bonds (roughly 1000:1 ratio in trans:cis populations). (wikipedia.org)
  • Both of these mechanisms for lowering the activation energy have been observed in peptidyl prolyl isomerases (PPIases), which are naturally occurring enzymes that catalyze the cis-trans isomerization of X-Pro peptide bonds. (wikipedia.org)
  • Nevertheless, peptide bonds can undergo chemical reactions, usually through an attack of an electronegative atom on the carbonyl carbon, breaking the carbonyl double bond and forming a tetrahedral intermediate. (wikipedia.org)
  • identify
  • The new AdvanceBio Peptide Mapping column can rapidly determine the primary protein structure and identify modifications with unparalleled speed and sensitivity. (technologynetworks.com)
  • Here, we identify the metal abstraction peptide (MAP) as an inhibitor of MMP-8 and demonstrate that tethering MAP to methacrylate polymers effectively inhibits catalysis. (ku.edu)
  • In biological applications, benzophenones have been used extensively as photophysical probes to identify and map peptide-protein interactions. (wikipedia.org)
  • consists
  • Cupiennin 1 family is the more well-known group, and consists of at least four peptides, such as cupiennin 1a, 1b, 1c, and 1d. (wikipedia.org)
  • citation
  • He also developed and published a peptide fingerprinting technique that was so popular that it became a citation classic Cleveland did postdoctoral work with William J. Rutter at the University of California at San Francisco from 1978 to 1981. (wikipedia.org)
  • results
  • Seamless generation of screen lists from peptide mapping results for easy similarity assessments or quality attributes monitoring across large datasets. (bruker.com)
  • significantly
  • A nonnative isomer of some peptide groups can disrupt the conformational folding significantly, either slowing it or preventing it from even occurring until the native isomer is reached. (wikipedia.org)
  • Earlier
  • Earlier, Hugh O. McDevitt and his coworkers mapped an Immune response-1 (Ir-1) locus influencing the level of antibody production against the synthetic polypeptide (T,G)-L-A into the Mhc. (wikipedia.org)
  • In fact, members of this family were discovered earlier in 1994 as member of the CSTX peptides. (wikipedia.org)
  • groups
  • However, X-Pro peptide groups tend to have a roughly 30:1 ratio, presumably because the symmetry between the C α {\displaystyle \mathrm {C^{\alpha }} } and C δ {\displaystyle \mathrm {C^{\delta }} } atoms of proline makes the cis and trans isomers nearly equal in energy (See figure, below). (wikipedia.org)
  • nonnative isomers of other peptide groups may not affect folding at all. (wikipedia.org)
  • matrix
  • The energy transfer is sufficient to promote the ionisation and transition of matrix molecules and peptides from the solid phase into the gas phase. (wikipedia.org)
  • similar
  • TGFβR1 (ALK5) and TGFβR2 have similar ligand-binding affinities and can be distinguished from each other only by peptide mapping. (wikipedia.org)
  • Due to its resonance stabilization, the peptide bond is relatively unreactive under physiological conditions, even less than similar compounds such as esters. (wikipedia.org)
  • However
  • However the AppliedBiosystems MAP substrate for peptide synthesis has been suggested, but not by anyone with much experience using it. (bio.net)