• sequences
  • I've been using a couple of commercially available libraries (7mer to 12mer) to look for peptide sequences which bind cell surface receptors. (bio.net)
  • A complication to this approach is that these peptide sequences need to first be identified, which can be challenging with viruses that undergo antigenic changes as a result of antigenic shift or drift, such as the influenza virus or HIV. (genscript.com)
  • These peptide sequences are used to predict the B- and T-cell epitopes to stimulate passive immunity. (genscript.com)
  • A limited repertoire with a well represented sequence space is always better than very large, degenerated libraries containing high levels of redundant codons and junk sequences with stop codons and unpaired cysteines leading to unwanted dimers. (abdesignlabs.com)
  • Xenome has leveraged the identification of venom peptide sequences into high quality synthesised peptides arrayed into a library suitable for high throughput screening. (biospace.com)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • Ribosome display begins with a native library of DNA sequences coding for polypeptides. (wikipedia.org)
  • cyclic peptides
  • We prepared a total of 372 reference pharmacophores, which were matched against 108,659 multiconformer cyclic peptides. (ucd.ie)
  • After normalization to exclude nonspecific cyclic peptides, the top hits notably are enriched for mimetics of turn structures, including a turn at the interaction surface of human α thrombin, and also feature several protein-binding peptides. (ucd.ie)
  • We have validated our method by testing cyclic peptides predicted to inhibit thrombin, a key protein in the blood coagulation pathway of important therapeutic interest, identifying a cyclic peptide inhibitor with lead-like activity. (ucd.ie)
  • We conclude that protein interfaces most readily targetable by cyclic peptides and related macrocyclic drugs may be identified computationally among a set of candidate interfaces, accelerating the choice of interfaces against which lead compounds may be screened. (ucd.ie)
  • The researchers identified the potential new therapeutic after testing more than a trillion small protein fragments, called cyclic peptides, to find one that could disable a vital enzyme in the disease-causing organisms, but leave similar enzymes in humans unscathed. (georgetown.edu)
  • bioactive peptides
  • In the course of this research, genomic and proteomic techniques have been used to investigate the bioactive peptides from the skin secretions of four American amphibian species: the Central American red-eyed leaf frog, Agalychnis callidryas~ the South American orange-legged leaf frog, Phyllomedusa hypochondrialis, Rohde's leaf frog, Phyllomedusa rohdei and the Giant Mexican leaf frog Pachymedusa dacnicolor. (bl.uk)
  • These discoveries of novel peptides from amphibian skin secretions have enriched our knowledge of bioactive peptides from this source and may provide the basis for several drug development programmes. (bl.uk)
  • Novel
  • Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. (neb.com)
  • The xdiscover™ library is a proprietary collection of more than 2000 primarily venom-derived peptides and is the result of a unique genomics capability that allows for the highly efficient discovery of novel peptides from cone snail venom tissue. (biospace.com)
  • Utilising its expertise in peptide chemistry and its novel technology platform, Xenome translates the evolutionary advantages inherent in venom peptides into new drug candidates. (biospace.com)
  • Presented is a novel protocol for the ligation of protected peptide segments that relies on a selective ruthenium-catalyzed cross metathesis reaction. (bl.uk)
  • binds
  • Cyclic peptide (middle) binds to iPGM (blue). (georgetown.edu)
  • When LPS is not present, the peptide binds too deeply within the active site, and the water cannot reach the carbonyl for its nucleophilic attack of the scissile bond. (wikipedia.org)
  • molecule
  • Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening the peptide-target interaction, also serves to detach the target molecule from the immobilization surface. (wikipedia.org)
  • sequence
  • so far I have been unsuccessful in locating a suitable sequence and am hoping to broaden my search by using a panel of libraries. (bio.net)
  • In this approach, a protein sequence database is used to calculate all putative peptide candidates in the given setting (proteolytic enzymes, miscleavages, post-translational modifications). (wikipedia.org)
  • The sequence search engines use various heuristics to predict the fragmentation pattern of each peptide candidate. (wikipedia.org)
  • Sequence database searching also demands vast search space, which still could not cover all possibilities of peptide dynamics, exhibiting limited efficiency post-translational modifications). (wikipedia.org)
  • We carry several methods to control the amount of diversity introduced at each position of the sequence and can mixed them to optimize the quality of your library. (abdesignlabs.com)
  • We routinely build libraries between 10(7) and 10(9) transformants depending on the number of variable positions and the degree of sequence space to be analyzed. (abdesignlabs.com)
  • selective
  • Bradykinin-like peptides (BRPs) exhibited significant and selective activities on a range of mammalian smooth muscle preparations and one was identified as a B2 receptor antagonist using the rat tail artery. (bl.uk)
  • However, the selective amplificability of DNA greatly facilitates library screening and it becomes indispensable for the encoding of organic compounds libraries of this unprecedented size. (wikipedia.org)
  • discovery
  • Xenome is a biotechnology company focused on the discovery and development of peptide-based therapeutics. (biospace.com)
  • Not only does this discovery raise hope for better treatments for many parasitic and bacterial diseases, it highlights the value of screening peptides in the search for ways to treat conditions that do not respond well-or have stopped responding-to more traditional chemical drug compounds. (georgetown.edu)
  • therapeutic
  • The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. (wikipedia.org)
  • Peptides are very useful as therapeutic and diagnostic substances. (wikipedia.org)
  • exclude
  • The experimental biologists can easily use the database as a virtual control to exclude possible target-unrelated peptides. (wikipedia.org)
  • candidates
  • The financial terms of the licenses remain confidential, however Xenome will be eligible to receive undisclosed milestone and royalty payments associated with the ongoing development of product candidates derived from the peptides. (biospace.com)
  • cell
  • By panning this library against a B lymphoctye cell line, a unique cell-binding and internalizing peptide was discovered. (wikipedia.org)
  • Further mechanistic studies of this peptide uncovered a dimerization "switch" that modulates the cell-penetrating activity. (wikipedia.org)
  • compound
  • citation needed] Researchers attempting to optimize the activity profile of a compound create a 'library' of many different but related compounds. (wikipedia.org)
  • vectors
  • Are peptides that are removed from their constrained vectors likely to still function in an unconstrained context? (bio.net)