Peptide antigens cd8. Medical search. Frequent questions

Scholars@Duke publication: Peptide/MHC tetramer-based sorting of CD8⁺ T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire. (duke.edu)
HBV-specific CD8 cells show altered MHC/peptide binding in the presence of high levels of circulating viral antigen. (ox.ac.uk)
Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)
This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. (jci.org)
To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. (jci.org)
peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
Cytoplasmic delivery and cross-presentation of proteins and peptides is necessary for processing and presentation of antigens for the generation of cytotoxic T cells. (edu.au)
We previously described the use of the 16 amino acid peptide penetratin from the Drosophila Antennapedia homeodomain (penetratin, Antp) to transport cytotoxic T lymphocyte epitopes derived from ovalbumin (OVA) or the Mucin-1 tumor-associated antigen into cells. (edu.au)
We have now shown that penetratin covalently conjugated to OVA protein and linked in tandem to CD4(+) and/or CD8(+) T-cell epitopes from OVA-stimulated T cells in vitro (B3Z T-cell hybridoma and OT-I and OT-II T cells). (edu.au)
Furthermore, interferon (IFN)-γ-secreting CD4(+) and CD8(+) T cells were induced which suppressed B16.OVA tumor growth in C57BL/6 mice. (edu.au)
Alternatively, 40 days after a boost immunization, the presence of CD4(+) help enhanced antigen-specific IFN-γ-secreting CD8(+) T cells and tumor protection in mice challenged with B16.OVA. (edu.au)
Monitoring after (CMV)-specific CD8(+) and CD4(+) T cells (4-6). (cdc.gov)
Smallpox Vaccination antigens are added to blood cells, allowing the simultane- ous analysis of both major histocompatibility complex class I and II restricted T-cell responses (7). (cdc.gov)
Quantitative analysis of hepatitis C virus-specific CD8+ T cells in peripheral blood and liver using peptide-MHC tetramers. (bdbiosciences.com)
Human CD8+CD28- suppressor T cells (Ts) are a subset of T cells generated in the course of in vitro and in vivo immunizations. (nih.gov)
Ts recognize MHC class I:peptide complexes and inhibit the reactivity of T helper cells (Th) with cognate antigen specificity. (nih.gov)
We have demonstrated for the first time that CD8+CD28- Ts represent a unique subset of regulatory cells that induces the differentiation of tolerogenic antigen-presenting cells, initiating a suppressive loop which results in the induction and spreading of Th unresponsiveness. (nih.gov)
Following specific binding of T cell receptors to viral and bacterial-derived peptides bound to MHC ( 5 ), or from neo-antigens ( 8 - 10 ), the appropriate T cells expand, resulting in the increased frequency of T cells carrying such receptors. (frontiersin.org)
Foreign antigens presented by MHC class I attract T-lymphocytes called killer T-cells (also referred to as CD8-positive or cytotoxic T-cells) that destroy cells. (wikipedia.org)
These particular antigens stimulate multiplication of T-helper cells (also called CD4-positive T cells), which in turn stimulate antibody-producing B-cells to produce antibodies to that specific antigen. (wikipedia.org)
Self-antigens are suppressed by regulatory T cells. (wikipedia.org)
When a foreign pathogen enters the body, specific cells called antigen-presenting cells (APCs) engulf the pathogen through a process called phagocytosis. (wikipedia.org)
They are then displayed by the antigen-presenting cells to CD4+ helper T cells, which then produce a variety of effects and cell-to-cell interactions to eliminate the pathogen. (wikipedia.org)
When bound, peptides are presented to T-cells. (wikipedia.org)
T-cells require presentation via MHC molecules to recognize foreign antigens-a requirement known as MHC restriction. (wikipedia.org)
T-cells have receptors that are similar to B-cell receptors, and each T-cell recognizes only a few MHC class II-peptide combinations. (wikipedia.org)
Here, we show that successful treatment of cervical carcinoma in mouse models with synthetic long peptide (SLP) vaccines induced influx of cytokine-producing CD8 T cells that strongly altered the numbers and phenotype of intratumoral macrophages. (aacrjournals.org)
These changes of the intratumoral myeloid composition coincided with macrophage recruitment by chemokines, including CCL2 and CCL5, and were completely dependent on a vaccine-induced influx of tumor-specific CD8 T cells. (aacrjournals.org)
Incubation of tumor cells with T cell-derived IFNγ and TNFα recapitulated the chemokine profile observed in vivo , confirming the capacity of antitumor CD8 T cells to mediate macrophage infiltration of tumors. (aacrjournals.org)
Here, we present a new generation of subunit vaccines targeting viral antigens to CD40-expressing antigen-presenting cells. (nature.com)
CD8 + T cells are essential components of the immune system. (biolegend.com)
Unconventional recognition of peptides by T cells and the implications for autoimmunity. (biolegend.com)
The T-cell mediated innate immune response is defined by the interaction between antigen presenting cells and T cells, through the Major Histocompatibility Complex (MHC) and the T cell receptor (TCR). (biolegend.com)
MHC molecules present a peptide to antigen-specific T cells that recognize this peptide. (biolegend.com)
However, by making a tetramer through a fluorescently labeled streptavidin conjugate, the complex binds to several TCRs, creating a more stable interaction and making it useful for flow cytometric detection of antigen specific T cells. (biolegend.com)
Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
Studies are also proposing a role for antimicrobial peptides and dendritic cells in the pathogenesis of psoriasis. (medscape.com)
CD8 + T cells play a critical role in immunity to viruses. (mdpi.com)
Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)
Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
They are activated on the surface of antigen -presenting cells, which mature during the innate immune responses triggered by an infection. (nih.gov)
Effector T cells act back to promote the expression of B7 proteins on antigen-presenting cells, creating a positive feedback loop that amplifies the T cell response. (nih.gov)
In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of antigen-expressing lentiviral vector, obviating the need for ex vivo transduction of dendritic cells. (jci.org)
Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. (bmj.com)
AIMS: To evaluate whether cytotoxic/suppressor T cells can be detected in paraffin wax embedded human tissue samples using antibodies to a synthetic CD8 peptide sequence. (bmj.com)
RESULTS: These antibodies specifically detected the native form of the CD8 polypeptide when tested by immunoprecipitation with radiolabelled T cells, and gave the expected staining pattern for cytotoxic/suppressor T cells in cryostat sections. (bmj.com)
CD8 positive cells could also be detected in paraffin wax embedded tissues. (bmj.com)
Autologous as well as allogeneic CD8 + T cells transduced with tumor antigen specific T cell receptors (TCR) may cause significant tumor lysis upon adoptive transfer. (oncotarget.com)
We hypothesized lysosome-associated membrane glycoprotein 1 (LAMP1, CD107a) to be a marker for fratricide in TCR transgenic CD8 + T cells. (oncotarget.com)
We identified HLA-A*02:01/peptide-restricted T cells directed against ADRB3 295 . (oncotarget.com)
After TCR identification, we generated HLA-A*02:01/peptide restricted TCR transgenic T cells by retroviral transduction and tested T cell expansion rates as well as A*02:01/peptide recognition and ES killing in ELISpot and xCELLigence assays. (oncotarget.com)
Comparison of peptide motive binding affinities revealed extended fratricide among ADRB3 295 specific TCR transgenic T cells in contrast to CHM1 319 . (oncotarget.com)
A vaccination strategy designed to enhance the immunogenicity of self-antigens that are overexpressed in tumor cells is to identify and slightly modify immunodominant epitopes that elicit T-cell responses. (aacrjournals.org)
The resultant T cells, however, must maintain their ability to recognize the native configuration of the peptide-MHC interaction on the tumor cell target. (aacrjournals.org)
These analyses enabled the design of peptide pools able to experimentally detect VACV-specific T cell responses and MPXV cross-reactive T cells in a cohort of vaccinated individuals. (jcvi.org)
Using flow cytometry to broadly assess the immunological cellular profile of recovered COVID-19 patients the authors showed the frequency of CD3 + cells was slightly increased in recovered COVID-19 patients relative to non-exposed controls, while no significant differences overall were observed in the frequencies of CD4 + or CD8 + T cells between the two groups. (nature.com)
The authors also detected IFNγ + SARS-CoV-2−specific CD8 + T cells in the majority of COVID-19 cases. (nature.com)
By doing this the authors hoped to understand which antigens are targeted by CD4 + and CD8 + T cells, whether the corresponding antigens are the same or different, and how do they compare to the antigens currently considered for COVID-19 vaccine development. (nature.com)
CD8 exists on the cell surface, where the CD8α chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8α. (peprotech.com)
Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (peprotech.com)
As simply diagrammed here, class I MHC molecules display peptides which are derived from intracellular, i.e. cytosolic or nuclear proteins, which as we'll see in a moment, become loaded in the endoplasmic reticulum and transported to the cell surface where they're recognized by CD8 T cells. (hstalks.com)
But these T cells must have already been activated by a different type of antigen presenting cell. (hstalks.com)
If the blood-testes barrier, which isolates germ cells from the contact with local immune system, is impaired by some reasons, autoimmunity to sperm antigens could be induced and results in the development of autoimmune orchitis. (benthamscience.com)
Specifically, CD8 and CD4 immune response is required for T cell activation by dendritic cells. (pdx.edu)
The antigen is expressed by all nucleated cells at varying levels. (bioxcell.com)
MHC Class I molecules present endogenously synthesized antigenic peptides to CD8 T cells. (bioxcell.com)
2015). "Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells" J Exp Med 212(8): 1153-1169. (bioxcell.com)
Upon arrival, T cells enlisted the support of nearly all brain-resident myeloid cells (microglia) by inducing proliferation and converting them into CD11c(+) antigen-presenting cells (APCs). (bioxcell.com)
Two-photon imaging experiments revealed that antiviral CD8(+) and CD4(+) T cells interacted directly with CD11c(+) microglia and induced STAT1 signaling but did not initiate programmed cell death. (bioxcell.com)
Viral integration may be curtailed when CD8+ T cells are triggered to kill infected CD4+ T cells through recognition of histocompatibility leukocyte antigen (HLA) class I-bound peptides derived from incoming virions. (ox.ac.uk)
We observe that ERAAP downregulation during infection leads to the presentation of the self-peptide FL9 on non-classical Qa-1b, thereby eliciting Qa-1b-restricted QFL T cells to proliferate in the liver and spleen of infected mice. (bvsalud.org)
The peptide repertoire presented by MHC class I molecules on the cell surface is essential for the immune surveillance of intracellular pathogens and transformed cells. (bvsalud.org)
It encodes cell surface molecules specialized to present antigenic peptides to the T-cell receptor (TCR) on T cells. (msdmanuals.com)
T cells that express CD8 molecules react with class I MHC molecules. (msdmanuals.com)
Because every nucleated cell expresses class I MHC molecules, all infected cells can act as antigen-presenting cells for CD8 T cells (CD8 binds to the nonpolymorphic part of the class I heavy chain). (msdmanuals.com)
Some class I MHC genes encode nonclassical MHC molecules, such as HLA-G (which may play a role in protecting the fetus from the maternal immune response) and HLA-E (which presents peptides to certain receptors on natural killer [NK] cells). (msdmanuals.com)
Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus In vivo. (ox.ac.uk)
In this study, we have used tetrameric major histocompatibility complex-peptide complexes to directly visualize antigen-specific cluster of differentration (CD)8+ T cells during the primary immune response to Epstein-Barr virus (EBV) infection in humans. (ox.ac.uk)
We show that massive expansion of activated, antigen-specific T cells occurs during the primary response to this virus. (ox.ac.uk)
The majority of the antigen-specific cells had an activated/memory phenotype, with expression of human histocompatibility leukocyte antigen (HLA) DR, CD38, and CD45RO, downregulation of CD62 leukocyte (CD62L), and low levels of expression of CD45RA. (ox.ac.uk)
After recovery from AIM, the frequency of antigen-specific T cells fell in most donors studied, although populations of antigen-specific cells continued to be easily detectable for at least 3 yr. (ox.ac.uk)
Modification with chimeric antigen receptors (CARs) endows T cells with tumor specific cytotoxicity and thus induce anti-tumor immunity against malignancies. (ijbs.com)
However, targeting solid tumors is more challenging than targeting B-cell malignancies with CAR-T cells because of the histopathological structure features, specific antigens shortage and strong immunosuppressive environment of solid tumors. (ijbs.com)
Advances in our understanding on the interaction between the immune system and tumor cells have contributed to the rapid development of novel therapeutic strategies based on chimeric antigen receptor (CAR) or T cell receptor (TCR) modified T cells. (ijbs.com)
Here, we review some of the recent advances and studies that highlight the roles of these changes as well as antigen presentation and stress response pathways in beta cells in the onset and propagation of the autoimmune process in type 1 diabetes. (springer.com)
and CD8 + T cells play a major role as beta cell killers [ 6 , 7 ]. (springer.com)
We advance the hypothesis that antigen presentation by beta cells, their stress responses and functional heterogeneity are critical factors that will provide clues towards solving the enigma posed by islet autoimmunity in type 1 diabetes. (springer.com)
The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)
Most T cells in the epithelium and adjacent to the damaged basal keratinocytes are activated CD8 + lymphocytes. (medscape.com)
Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
Activated CD8 + T cells (and possibly keratinocytes) may release cytokines that attract additional lymphocytes into the developing lesion. (medscape.com)

The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
In addition, the CD4 and CD8 co-receptors play important parts in the signaling process, as illustrated in Figure 24-64 . (nih.gov)
The signaling events initiated by the binding of peptide-MHC complexes to T cell receptors (signal 1). (nih.gov)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (peprotech.com)
It's the recognition of these peptide MHC complexes by the T cell antigen receptors that initiates most immune responses. (hstalks.com)

Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
They function through TCR recognition of antigens (peptide epitopes) presented by the class I MHC (HLA in humans) molecules. (biolegend.com)
Recently, the first set of naturally presented human leukocyte antigen Class I (HLA-I) epitopes of MuV was identified by us. (mdpi.com)
Heteroclitic peptides are sequence variants of native peptide epitopes that stimulate T cell responses superior to the native epitope. (mun.ca)
Twenty-four variant peptides were generated from reference human histocompatibility-linked leukocyte antigen (HLA)-A2- restricted peptide epitopes Nef 8391, Nef 135143, Gag 433440 and Gag 7785 with conservative and semi-conservative amino acid substitutions at positions 3, 5 and 7 or 3, 5 and 8 of Gag 433440. (mun.ca)

The human leukocyte antigen (HLA) system or complex is a complex of genes on chromosome 6 in humans which encode cell-surface proteins responsible for regulation of the immune system. (wikipedia.org)
The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplants. (wikipedia.org)
These peptides are produced from digested proteins that are broken down in the proteasomes. (wikipedia.org)
Aside from the genes encoding the six major antigen-presenting proteins, many other genes, many involved in immune function, located on the HLA complex. (wikipedia.org)
Proteins from the pathogen are digested into small pieces (peptides) and loaded on to HLA antigens (to be specific, MHC class II). (wikipedia.org)
This study provides the first evidence that homocitrullinated proteins in tumors can also be targets for CD8 T-cell responses. (bmj.com)
This research suggests that post-translational modification of proteins provide efficient targets in tumors for both CD4 and CD8 T-cell-mediated therapies. (bmj.com)
However, limited data are available about sequence variability in CD8 + T cell epitope regions of mumps virus (MuV) proteins. (mdpi.com)
This peptide-MHC complex signals through the T cell receptor and its associated proteins. (nih.gov)
The expression of B7 proteins on an antigen-presenting cell is induced by pathogens during the innate response to an infection. (nih.gov)
It is associated with a complex of invariant transmembrane proteins called CD3 , which transduces the binding of the peptide- MHC complex into intracellular signals ( Figure 24-63 ). (nih.gov)
Endogenous synthesis of antigen results in efficient proteolytic peptide processing and presentation of peptide antigen on MHC class I proteins. (jci.org)
In contrast, class II MHC molecules capture and display peptides derived from exogenous proteins, i.e. proteins taken up into the cell by one or more forms of endocytosis, for example, bacterial toxins like tetanus toxin. (hstalks.com)
These proteins attach to protein fragments (peptides) outside the cell. (medlineplus.gov)
MHC class II proteins display these peptides to the immune system. (medlineplus.gov)

Successful recognition of antigenic peptides bound to Major Histocompatibility Complexes (pMHCs) requires specific binding of the TCR to these complexes ( 5 - 7 ), which in turn modulates the cell's fitness, clonal expansion, and acquisition of effector properties ( 7 ). (frontiersin.org)

From a leukemia cell line, we identified a CDK4-derived peptide epitope, UNC-CDK4-1 (ALTPVVVTL), that bound HLA-A*02:01 with high affinity and could induce CD8⁺ T-cell responses in vitro. (duke.edu)
The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
Importantly, in vivo the homocitrullinated aldolase specific response was associated with efficient CD8 dependent antitumor therapy of the aggressive murine B16 tumor model indicating that this epitope is naturally presented in the tumor. (bmj.com)
In the present study, sequences of 40 CD8 + T cell epitope candidates, including previously and newly identified, obtained from Jeryl-Lynn mumps vaccine strains were compared with genomes from 462 circulating MuV strains. (mdpi.com)
Injection of a lentiviral vector encoding an MHC class I-restricted T cell epitope of lymphocytic choriomeningitis virus (LCMV) and CD40 ligand induced an antigen-specific cytolytic CD8 + T lymphocyte response that protected the mice from infection. (jci.org)
Here, we leveraged the epitope information available in the Immune Epitope Database (IEDB) on VACV to predict potential MPXV targets recognized by CD4 and CD8 T cell responses. (jcvi.org)
Through interrogation of the pre-integration immunopeptidome, we obtain proof of early presentation of a virion-derived HLA-A∗02:01-restricted epitope, FLGKIWPSH (FH9), located in Gag Spacer Peptide 2 (SP2). (ox.ac.uk)

Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
The human leukocyte antigen (HLA) system (the major histocompatibility complex [MHC] in humans) is an important part of the immune system and is controlled by genes located on chromosome 6. (msdmanuals.com)
The HLA-DRB1 gene is part of a family of genes called the human leukocyte antigen (HLA) complex. (medlineplus.gov)

The BD FastImmune™ CD8 Intracellular Cytokine Detection Kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD8 + T lymphocytes in whole blood. (bdbiosciences.com)
HLAs corresponding to MHC class II (DP, DM, DO, DQ, and DR) present antigens from outside of the cell to T-lymphocytes. (wikipedia.org)

The immense diversity of the T-cell receptor (TCR) enables specific antigen recognition ( 3 , 4 ). (frontiersin.org)

Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)

Healthy humans develop spontaneous CD8+ T cell responses to melanoma associated antigens (MA) expressed by normal melanocytes. (mdpi.com)
Defining antigen targets to dissect vaccinia virus and monkeypox virus-specific T cell responses in humans. (jcvi.org)

The lichen planus antigen is unknown, although it may be a self-peptide (or altered self-peptide), in which case lichen planus would be a true autoimmune disease. (medscape.com)

Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. (bdbiosciences.com)
In contrast, many TCR responses are characterized by a high level of cross-reactivity with single TCRs binding a large number of MHC-bound peptides, and single peptides binding a large number of TCRs ( 16 , 17 ). (frontiersin.org)
Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8(+) T cell response. (expasy.org)

Both linear and cyclic peptides have been explored as trafficking moiety due to ease of synthesis, structural simplicity, and low probability of undesirable immunogenicity. (biosyn.com)

Testing of T cell-based cancer therapeutics often involves measuring cancer antigen-specific T-cell populations with the assumption that they arise from in vivo clonal expansion. (duke.edu)
Mapping recurrent TCRβ clonotype sequences onto TCRβ repertoires can help confirm or refute antigen-specific T-cell expansion in vivo. (duke.edu)
For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)
CD8 responses were assessed in vitro for cytotoxicity and in vivo tumor therapy. (bmj.com)
Results Homocitrullinated peptides from aldolase and cytokeratin were identified, that stimulated CD8-mediated responses in vivo. (bmj.com)
CD8 and CD4 immune response was measured in vivo using flow cytometry. (pdx.edu)

Eight classical TB vaccine candidate antigens, 51 DosR regulon encoded antigens, 23 TB reactivation antigens, 5 TB resuscitation promoting factors (rpfs), 6 starvation and 24 other stress response-associated TB antigens were evaluated in the study. (biomedcentral.com)
These nanoparticles, when functionalized with peptides and other molecules, can be used as vaccine in cancer treatment. (pdx.edu)
How does the fact that there's an extra step involved in producing the vaccine's protein-based antigen(s) within the human body somehow mean that the final result isn't a true vaccine? (educatetruth.com)

Do I need to know the sequence of the UV-labile peptide? (biolegend.com)
The sequence of the UV-labile peptide is not needed to use the reagent. (biolegend.com)
METHODS: Polyclonal and monoclonal antibodies were raised against a 13 amino acid peptide sequence from the cytoplasmic portion of the alpha chain of the human CD8 molecule. (bmj.com)

By any rational standard, the mRNA vaccines are true vaccines in every sense of the word since they end up educating the human immune system to recognize a specific type of viral protein antigen which then causes this now educated immune system to specifically target the COVID-19 virus prior to an actual infection by the live virus. (educatetruth.com)

Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)
Human tumor samples were analyzed by targeted mass spectrometry for presence of homocitrullinated peptides. (bmj.com)

While murine cytomegalovirus (MCMV) has multiple strategies for manipulating the antigen processing pathway to evade immune responses, the host has also developed ways to counter viral immune evasion. (bvsalud.org)

The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)
In this issue of the JCI , two separate studies on MHC-bound peptides reopen the debate on the utility of peptides for the purposes of vaccination and treatment of autoimmune diseases. (jci.org)
The image off to the side shows a piece of a poisonous bacterial protein (SEI peptide) bound within the binding cleft portion of the HLA-DR1 molecule. (wikipedia.org)
In the illustration far below, a different view, one can see an entire DQ with a bound peptide in a similar cleft, as viewed from the side. (wikipedia.org)
We developed a technology where dissociation (koff rate) of truly monomeric peptide-major histocompatibility complex (pMHC) molecules bound to surface-expressed TCRs can be monitored by real-time microscopy in a highly reliable manner. (mdc-berlin.de)
Signal 1 is provided by a foreign peptide bound to an MHC protein on the surface of the presenting cell. (nih.gov)

We employ new Natural Language Processing (NLP) based methods to predict whether any TCR and peptide bind. (frontiersin.org)
We combined large-scale TCR-peptide dictionaries with deep learning methods to produce ERGO (pEptide tcR matchinG predictiOn), a highly specific and generic TCR-peptide binding predictor. (frontiersin.org)

Two-color staining with the same allele/peptide combination is recommended for flow cytometry analysis to increase the resolution and specificity of the assay. (biolegend.com)

Different Mycobacterium tuberculosis (M.tb) infection phase-dependent antigens may be differentially recognized in infected and diseased individuals and therefore useful as diagnostic tools for differentiating between M.tb infection states. (biomedcentral.com)
In this study, we assessed the diagnostic potential of 118 different M.tb infection phase-dependent antigens in TB patients and household contacts (HHCs) in a high-burden setting. (biomedcentral.com)
The expression or unmasking of the lichen planus antigen may be induced by drugs (lichenoid drug reaction), contact allergens in dental restorative materials or toothpastes (contact hypersensitivity reaction), mechanical trauma (Koebner phenomenon), viral infection, or other unidentified agents. (medscape.com)

For the final set of experiments in the article, the authors synthesized sets of overlapping peptides spanning the entire open reading frame of SARS-CoV-2 and pooled them separately so that each pool would represent one polypeptide. (nature.com)

Contents: Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Solution, EDTA Solution, BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x). (bdbiosciences.com)
Being raised in rabbits, the polyclonal antibodies were also useful for double labelling for CD8 in conjunction with monoclonal antibodies. (bmj.com)
CONCLUSIONS: These findings provide further evidence that antibodies against leucocyte associated antigens, capable of reacting on paraffin wax embedded tissue, can be produced by immunisation with synthetic peptide sequences. (bmj.com)
Similarly, since spermatozoa are not self and isoantigens to females, if they are exposed repeatedly to sperm antigens by coitus, it is not surprising that isoimmunity to sperm antigens could be induced and develop antisperm antibodies (ASA) in females. (benthamscience.com)

Nanovaccines can improve antigen presentation, targeted delivery, stimulation of the body's innate immune system, and a strong T-cell response without putting people at risk. (researchgate.net)
This is a talk on antigen processing and presentation, and in particular how it's regulated by innate immune signals. (hstalks.com)
Antigen presentation refers to the display of short process peptides on so-called MHC, or major histocompatibility complex molecules. (hstalks.com)
Introduction: Critical steps in Major Histocompatibility Complex Class I (MHC-I) antigen presentation occur in the endoplasmic reticulum (ER). (bvsalud.org)
Discussion: We propose a mechanism for how ERAP1 and ERAP2 synergize to modulate their respective activities and shape the MHC-I peptidome by generating optimal peptides for presentation. (bvsalud.org)

Glycoprotein (GP) is the major protective antigen of EBOV, and can generate virus-like particles (VLPs) by co-expression with matrix protein (VP40). (frontiersin.org)
PeproTech's CHO cell-derived Recombinant Human sCD8α is a monomeric glycoprotein of 161 amino acid residues, which corresponds to the extracellular domain of CD8α. (peprotech.com)

The first US Food and Drug Administration-approved immunotherapy for type 1 diabetes, the anti-CD3 antibody teplizumab, delays symptomatic type 1 diabetes onset by 2-3 years [ 4 ], opening the door for the future clinical application of antigen-specific approaches with potentially superior immunoregulatory properties [ 5 ]. (springer.com)

CD8 is thought to play a role in the process of T-cell mediated killing. (lu.se)

Class I MHC molecules generally have peptides between 8 and 10 amino acid residues. (hstalks.com)
While ERAP1 efficiently trims peptides longer than 9 residues, ERAP2 preferentially trims peptides shorter than 9 residues. (bvsalud.org)

This analysis, using peptide/MHC tetramers, is often ambiguous. (duke.edu)

Notably, vaccination with DREP-GP and DREP-VP40, which produces both GP and VP40 antigens, induced a significantly higher level of anti-GP IgG2a antibody and increased IFN-γ secreting CD8 + T-cell responses relative to vaccination with DREP-GP or DREP-VP40 vector alone. (frontiersin.org)

Together, they form a functional protein complex called the HLA-DR antigen-binding heterodimer. (medlineplus.gov)

Whole protein and defined CD8(+) and CD4(+) peptides linked to penetratin targets both MHC class I a. (edu.au)
HLAs corresponding to MHC class I (A, B, and C), all of which are the HLA Class1 group, present peptides from inside the cell. (wikipedia.org)
Class II molecules accommodate longer peptides, about 14 - 20 amino acids 1 . (biolegend.com)
Seen here is a class I MHC molecule with the short 8-residue peptide found in a peptide binding groove. (hstalks.com)
Whereas class II MHC molecules display considerably longer peptides due to a more open ended peptide-binding groove. (hstalks.com)
MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) associated with ß2 microglobulin (11.5 kDa). (bioxcell.com)
The endoplasmic reticulum aminopeptidase associated with antigen processing (ERAAP) plays a crucial role in shaping the peptide-major histocompatibility complex (MHC) class I repertoire and maintaining immune surveillance. (bvsalud.org)
Individual serologically defined antigens encoded by the class I and II gene loci in the HLA system are given standard designations (eg, HLA-A1, -B5, -C1, -DR1). (msdmanuals.com)
CD8 alpha chains binds to class I MHC molecules alpha-3 domains. (lu.se)

If the immune system recognizes the peptides as foreign (such as viral or bacterial peptides), it triggers a response to attack the invading viruses or bacteria. (medlineplus.gov)

Single amino acid substitutions were introduced to the CAP1 peptide (YLSGANLNL), an immunogenic HLA-A2 + -binding peptide derived from human carcinoembryonic antigen (CEA). (aacrjournals.org)

The mRNA vaccines can encode multiple antigens, strengthening the immune response against pathogens and enabling the targeting of multiple microbial variants [19] . (researchgate.net)

We can distinguish the two classes of MHC molecule by the types of peptide they present. (hstalks.com)

Previous studies identified a protective role for the Toxoplasma CD4+ T cell-eliciting peptide AS15 (AVEIHRPVPGTAPPS) in C57BL/6J mice. (bvsalud.org)

The DCs are then pulsed with synthetic peptide or cell lysate and matured with cytokine or TLR agonists. (jci.org)

The induction of these responses was directly mediated by the penetratin peptide as linking a nonspecific 16-mer peptide to OVA or mixing did not induce CD8(+) or CD4(+) T-cell responses in vitro. (edu.au)

Liu B, Chen W, Evavold BD, Zhu C (2014) Accumulation of dynamic catch bonds between TCR and agonist peptide-MHC triggers T cell signaling. (meduniwien.ac.at)
Therefore, the analogue CAP1-6D (YLSGADLNL, Asn at position 6 replaced by Asp) meets the criteria of a CTL enhancer agonist peptide. (aacrjournals.org)

Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution. (lu.se)

Anatomy25

Organisms9

Diseases2

Chemicals and Drugs158

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes40

Disciplines and Occupations1

Information Science3

Health Care2

Cells93

Receptors6

Epitopes6

Proteins15

Complexes1

Epitope7

Leukocyte antigen3

Lymphocytes2

Recognition1

Induces1

Humans2

Self-peptide1

Reactivity3

Immunogenicity1

Vivo6

Vaccine3

Sequence3

Recognize1

Mass spectrometry2

Immune Responses1

Bound8

Methods2

Flow cytometry1

Infection3

Polypeptide1

Antibodies4

Presentation5

Glycoprotein2

Immunotherapy1

Cell-mediated1

Residues2

Tetramers1

Vaccination1

Heterodimer1

Class9

Bacterial1

Immunogenic1

Vaccines1

Molecule1

Protective1

Synthetic1

Induce1

Agonist2

Human1