• Falk Schlaudraff (Institute of General Physiology of the University of Ulm, Germany) used modern laser microdissection methods to isolate and analyze cells from post mortem tissue specimens taken from M. Parkinson patients in order to gain molecular insight into the disease. (leica-microsystems.com)
  • As part of his Ph.D. he analyzed the gene expression of human dopaminergic neurons, which he isolated from post mortem midbrain tissue of Morbus Parkinson patients and control subjects using a laser microdissection system from Leica Microsystems. (leica-microsystems.com)
  • Our analysis, for example the RNA quality of the specimens, showed that the quality of the results is not influenced by the staining process and laser microdissection. (leica-microsystems.com)
  • A) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV -laser-microdissection of the entire area. (leica-microsystems.com)
  • Laser microdissection microscopy has become a rising tool to assess gene expression profiles of pure cell populations. (biomedcentral.com)
  • 4. Evaluate the expression of p16, PTEN and CCDN1 mRNA in parafin embedded tissue, obtained by laser microdissection. (fapesp.br)
  • Single-cell laser microdissection was utilized to identify the minimal number of tumor cells needed for mutation detection. (takarabio.com)
  • Conversely, OCT-embedded (Optimal Cutting Temperature compound) specimens can undergo laser microdissection (LMD) to capture and study specific cell types separately from the cell mixture. (usuhs.edu)
  • Methods: We combined immunohistochemistry, laser microdissection, and single-cell multiplex PCR to characterize T-cell subtypes and identify paired TCRα and TCRβ chains from individual brain-infiltrating T cells in frozen brain sections. (uni-muenchen.de)
  • NCEZID scientists used a technique known as laser microdissection to pinpoint the specific cells where Zika virus might be persisting and replicating . (cdc.gov)
  • One of the aims of my research was to develop optimized qPCR (quantitative polymerase chain reaction)-based methods to enable valid comparison of the gene expression of promising gene candidates in individual neurons from human post mortem tissue from M. Parkinson patients with the gene expression of the same neurons from healthy control subjects. (leica-microsystems.com)
  • Prognostic significance of a polymerase chain reaction-detectable dominant T-cell clone in cutaneous lesions of patients with mycosis fungoides. (jamanetwork.com)
  • Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. (jamanetwork.com)
  • Diss TCWatts MPan LXBurke MLinch DIsaacson PG The polymerase chain reaction in the demonstration of monoclonality in T-cell lymphomas. (jamanetwork.com)
  • Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sézary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). (jamanetwork.com)
  • Scheller UMuche JMSterry WLukowsky A Detection of clonal T cells in cutaneous T-cell lymphoma by polymerase chain reaction: comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels. (jamanetwork.com)
  • Tok JSzaboles MJSilvers DNZhong JMatsushima AY Detection of a clonal T-cell receptor gamma chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: correlation of histologic findings with PCR/DGGE. (jamanetwork.com)
  • Pan LXDiss TCPeng HZIsaacson PG Clonality analysis of defined B-cell populations in archival tissue section using microdissection and the polymerase chain reaction. (jamanetwork.com)
  • Utility of microdissection and polymerase chain reaction for the detection of immunoglobulin gene rearrangement and translocation in primary intraocular lymphoma. (jamanetwork.com)
  • L&H cells from lymphocyte predominant HL cases and centroblasts from lymphadenites were micromanipulated and analysed for AID expression by quantitative real time polymerase chain reaction. (bmj.com)
  • Methods Whole-exome sequencing was performed on ECH lesions from 12 patients (discovery cohort) and droplet digital polymerase-chain-reaction (ddPCR) was used to confirm the identified mutation in 46 additional cases (validation cohort). (bmj.com)
  • In 4 patients, polymerase chain reaction analysis of T-cell receptor-gene rearrangement after laser-based microdissection of the specimen revealed that the monoclonal population of T lymphocytes was confined mainly to the epidermis. (unipv.it)
  • We explored whether JAK2V617F was present in ECs in the vessels of polycythemia vera (PV) patients with BCS using laser capture microdissection followed by nested polymerase chain reaction or reverse-transcribed polymerase chain reaction. (uludag.edu.tr)
  • citation needed] Because protein phosphorylation is one of the most studied protein modifications, many "proteomic" efforts are geared to determining the set of phosphorylated proteins in a particular cell or tissue-type under particular circumstances. (wikipedia.org)
  • We developed a qPCR-based platform that could be used to isolate individual cells from native tissue and obtain highly comparable gene expression analyses. (leica-microsystems.com)
  • Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
  • This procedure, which involves the use of enzymes for the separation of the pancreatic endocrine cells from the surrounding exocrine tissue, may introduce unwanted effects on the former. (tu-dresden.de)
  • Our approach relies on the use of Laser Capture Microdissection (LCM), a technique that utilizes a laser beam to physically cut out the desired endocrine cells from the surrounding tissue" explains Marko Barovic, PhD student in the Solimena lab of the Paul Langerhans Institute Dresden and first author of the review. (tu-dresden.de)
  • Using the LCM technique, islets can be isolated from only a few hundred micrograms of pancreatic tissue procured during routine processing of surgical specimens of patients undergoing pancreatic surgery. (tu-dresden.de)
  • Oligonucleotide microarray technology is a powerful application to measure genome-wide changes in mRNA expression levels in various tissue samples or cell lines. (biomedcentral.com)
  • However, use of whole tissue homogenates unavoidably results in averaging of expression levels of different cell types present in the tissue. (biomedcentral.com)
  • Samples were procured by LCM from HCC and patient-matched normal liver tissue surgically resected from 4 patients. (geneticsmr.org)
  • We first asked whether a cell-autonomous mechanism could contribute to exhaled biomarkers, so we began by searching for thematic differences in metabolic gene expression between EAC and relevant control tissue in archived transcriptomic datasets. (nature.com)
  • In this Webinar, after a technical presentation by Dr. Tamara Straube, Dr. Giovannetti discuss the advantages of using LMD techniques for precise, contamination-free isolation of specific cell types in various applications, such as using pancreatic cancer tissue sections from patients and orthotopic xenografts. (cancerpharmacology.org)
  • PATIENTS: Nineteen children with cutaneous findings in the first 4 weeks of life and subsequently diagnosed with LCH based on compatible tissue histologic analysis, confirmed by electron microscopy and/or immunohistochemical analysis. (thedoctorsdoctor.com)
  • Laser capture microdissection (LCM) was carried out to capture and characterise subgroups of tissue cells. (bmj.com)
  • Such inflammatory tissue damage is responsible for morbidity and mortality in patients. (ersjournals.com)
  • The key consequences of such tissue destruction are morbidity and mortality in TB patients. (ersjournals.com)
  • This review will focus on the contribution of high-throughput genomic and proteomic approaches to the study of fibrogenesis and fibrosis progression, concentrating on the most prevalent human chronic liver diseases and findings from animal models in liver tissue, isolated liver cells, cell lines and serum. (biomedcentral.com)
  • Using available computational methods and developing novel specific algorithms, we propose to use large genomic alterations for analysing metastatic migration patterns in up to 50 lung cancer patients enrolled in the national research autopsy study PEACE, for which an unprecedented number of metastatic samples is available. (ucl.ac.uk)
  • Then as a clinical lecturer in Liverpool while training in paediatric infectious diseases his research took a more basic approach, to understand the mechanisms of his PhD observations: developing in vitro methods and using laser capture microdissection to examine specific cell populations using transcriptomics in patient samples. (gla.ac.uk)
  • Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
  • In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
  • Patients and Methods: The outcome of 127 patients with advanced NSCLC who recieved pemetrexed or docetaxel at second- or third-line therapy was retrospectively analyzed. (iiarjournals.org)
  • Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. (nature.com)
  • Performance of four modern whole genome amplification methods for copy number variant detection in single cells. (takarabio.com)
  • [ 2 ] The origin of the HL Reed-Sternberg cells (HL-RSC) became clear with the introduction of microdissection and single-PCR methods. (medscape.com)
  • Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR, respectively. (besjournal.com)
  • Methods: Proteins were extracted from FF and OCT-embedded invasive breast tumors from 5 female patients. (usuhs.edu)
  • Other strategies include the use of varenicline, a partial agonist of the nicotinic acetylcholine receptor, counseling and other socio-economic methods including taxation, advertisement and legislative measures, such as lowering the amount of nicotine in cigarettes to non-addictive levels, a policy recently announced by the US Food and Drug Administration (FDA) Cancer cells cause mutations in our body, and mutations change the way our cells behave. (bestgrademiners.com)
  • Indeed, mass spectrometry is the most powerful method for analysis of proteomes, both in large samples composed of millions of cells and in single cells. (wikipedia.org)
  • Background: Proteomic studies are typically conducted using flash-frozen (FF) samples utilizing tandem mass spectrometry (MS). However, FF specimens are comprised of multiple cell types, making it difficult to ascertain the proteomic profiles of specific cells. (usuhs.edu)
  • [ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)
  • Using microdissection, Gli1 protein and mRNA expression were positively correlated with one another. (bcm.edu)
  • Year-1: Validation of HNMT as a predictive marker for Herceptin-Resistance (HR) in breast cancer patients Aim 1: Determination of the HNMT and HER2 mRNA expression level in BC cells 1. (tmu.edu.tw)
  • Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. (nature.com)
  • Oral squamous cell carcinoma (OSCC) is the most common type of head and neck malignant tumor and is ranked the eighth leading cause of cancer worldwide. (nature.com)
  • Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
  • Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)
  • 6 Recent studies of AID protein expression using western blot analysis showed that AID expression is largely restricted to normal and transformed GC B cells and correlates well with the expression of AID transcripts. (bmj.com)
  • The protein expressions of p53 and unc-51 like kinase 2 (ULK2) in CRC cells were detected by western blot. (besjournal.com)
  • Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
  • The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
  • However, the landscape of heteroplasmy across cell types within tissues and its influence on phenotype expression in affected patients remains largely unexplored. (jci.org)
  • LCM was able to accurately capture 50-200 cells from HCC and control tissues. (geneticsmr.org)
  • In our research we propose to use unique primary pancreatic cancer cell cultures and tissues library in order to establish a robust imaging platform for in vivo screening of selected innovative drugs, as well as to further delineate the biology and genetic characteristics of this tumor. (cancerpharmacology.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Then the cells start growing abnormally, which then affect our tissues. (bestgrademiners.com)
  • 2. Isolation of the HR-and Herceptin sensitive (HS)-tumor cells from the BC tissues for genotyping analysis by laser capture microdissection (LCM). (tmu.edu.tw)
  • Evaluate HNMT/HER2 complex formation in tumor tissues as a predictor for HR in BC patients. (tmu.edu.tw)
  • Establish the primary cultured cell lines from the HR cell-xenografted tumor tissues during different stages of Herceptin® treatment. (tmu.edu.tw)
  • This involves looking under the microscope and guiding a laser to cut away specific cells from areas of the brain and placental tissues, so scientists could measure the amount of Zika virus genetic materials (RNA) in specific cells. (cdc.gov)
  • This cohort of patients will also include samples obtained from the national TRACERx lung study, providing a unique opportunity to study these genetic changes throughout the disease course, from diagnosis to death. (ucl.ac.uk)
  • Bottaro MBerti EBiondi AMigone NCrosti L Heteroduplex analysis of T-cell receptor gamma gene rearrangements for diagnosis and monitoring of cutaneous T-cell lymphomas. (jamanetwork.com)
  • Chhanabhai MAdomat SAGascoyne RDHorsman DE Clinical utility of heteroduplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders. (jamanetwork.com)
  • ral drug doses, serial determinations was given to seven patients, and Immunoblot interpretation criteria for sero- of HHV-6 DNA by polymerase chain immunoglobulin was given to one diagnosis of early Lyme disease. (cdc.gov)
  • Volatile aldehydes are enriched in esophageal adenocarcinoma (EAC) patients' breath and could improve early diagnosis, however the mechanisms of their production are unknown. (nature.com)
  • Analysis of PicoPLEX WGA products provided a correct diagnosis for 16/16 cells, with detection of 16/16 individual aneuploidies. (takarabio.com)
  • The results of a multiorgan workup performed at the time of diagnosis were predictive of which patients in this cohort manifested multisystem disease. (thedoctorsdoctor.com)
  • The presence of neoplastic islands, classified as large or small according to the number of cells in the ITF, has been described as the most aggressive pattern compared to tumors with a more uniform growth pattern, as tumor invasion occurs in a more widespread manner as cellular islands or single cells 4 . (nature.com)
  • When inoculated in immunodeficient mice, the DUSP10-overexpression NSCLC cells formed larger tumors compared to control cells. (bvsalud.org)
  • Researchers have refined a method they previously developed for capturing and analyzing cancer cells that break away from patients' tumors and circulate in the blood. (sciencedaily.com)
  • Circulating tumor cells, or CTCs, play a crucial role in cancer metastasis, spreading from tumors to other parts of the body, where they form new tumors. (sciencedaily.com)
  • Normal cells grow when your body needs them, and die when your body does not need them any longer.Cancer is composed of abnormal cells that grow into tumors. (bestgrademiners.com)
  • Do not forget to cite it if it is not your own.Cancer cells form tumors. (bestgrademiners.com)
  • However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
  • I detected an orchestrated change of gene expression in the selective dopaminergic neurons of M. Parkinson patients. (leica-microsystems.com)
  • Here too, we noticed a change in the expression of some of the examined genes of M. Parkinson patients. (leica-microsystems.com)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • The expression profile of the cell type of interest will therefore be masked or even lost because of the surrounding cells. (biomedcentral.com)
  • The increased growth of DUSP10-overexpression NSCLC cells was associated with increased expression of tumor-promoting cytokines including IL-6 and TGFß. (bvsalud.org)
  • Importantly, higher DUSP10 expression was associated with poorer prognosis of NSCLC patients. (bvsalud.org)
  • For example, a second study examining differential expression of hedgehog network genes in at least 8 patient matched samples total showed SHH, PTCH1 and GLI1 expression (Mukherjee et al. (bcm.edu)
  • 7 Several studies analysing AID expression and mutation patterns in V gene rearrangements in various B cell non-HLs showed that, although somatic hypermutation activity is strictly dependent on AID, the expression of this protein does not necessarily indicate ongoing somatic hypermutation. (bmj.com)
  • 11 To clarify this issue for lymphocyte predominant HL we quantitatively examined the expression of AID in the L&H cells of six patients with lymphocyte predominant HL at the transcription level, because suitable antibodies for immunohistochemistry are not yet available. (bmj.com)
  • Despite high protein expression and enzymatic activity of cytochrome P450 1B1 (CYP1B1) in renal cell cancer (RCC), its functional significance has not been elucidated. (oncotarget.com)
  • Instead, alteration of CYP1B1 expression regulates the chemosensitivity of RCC cells to docetaxel suggesting its critical contribution to the chemoresistance. (oncotarget.com)
  • Restoration of docetaxel resistance by exogenous expression of CYP1B1 in miR-200c-over-expressing cells indicates that CYP1B1 is a functional target of miR-200c. (oncotarget.com)
  • bioinformatics analysis demonstrated that activin induces a gene expression pattern in skin macrophages that resembles the phenotype of tumor\associated macrophages in different malignancies, thereby promoting angiogenesis, cell migration and proteolysis. (citiesofdata.org)
  • This expression pattern is similar to the one observed in human SCCs (Antsiferova in tumor cells of AK and SCC patients isolated by laser capture microdissection (Lambert by qRTCPCR. (citiesofdata.org)
  • High levels of K17 expression were significantly associated with decreased patient survival. (medscape.com)
  • Thus, the goal of this study was to determine if K17 expression is a negative prognostic biomarker associated with decreased survival of patients with endocervical adenocarcinoma. (medscape.com)
  • Distinct genes are expressed in different cell types, which means that even the basic set of proteins produced in a cell must be identified. (wikipedia.org)
  • Clonal rearrangements of T-cell receptor genes in mycosis fungoides and dermatopathic lymphadenopathy. (jamanetwork.com)
  • Langerak AWScczepanski Tvan der Burg MWolvers-Tettero ILvan Dongen JJ Heteroduplex PCR analysis of rearranged T-cell receptor genes for clonality assessment in suspect T-cell proliferations. (jamanetwork.com)
  • General objective: To evaluate the cell-cycle proteins, the supressor genes and oncogenes in acral lentiginous melanomas. (fapesp.br)
  • 2003). More recently, array comparative genomic hybridization (CGH) analyses indicate that genomic loss at the Ptch1 locus was the fourth most commonly detected change among the tumor suppressor genes identified in the study, occurring in 19 percent of human breast cancers and 33 percent of breast cancer cell lines (Naylor et al. (bcm.edu)
  • In vitro experiments in endothelial cells demonstrated that the GJA4 mutation activated SGK-1 signalling that in turn upregulated key genes involved in cell hyperproliferation and the loss of arterial specification. (bmj.com)
  • Germinal-center B-cells undergo clonal expansion and activate the process of somatic hypermutation that introduces mutations at a high rate into rearranged immunoglobulin variable genes. (medscape.com)
  • With this technology, we are getting closer to the goal of a widely clinically applicable liquid biopsy, where we can sample cancer cells by a simple blood draw and understand the genes that allow them to grow," said Dr. Antoni Ribas, a professor of medicine in the division of hematology-oncology, a Jonsson Cancer Center member and one of Tseng's key collaborators. (sciencedaily.com)
  • It is more complicated than genomics because an organism's genome is more or less constant, whereas proteomes differ from cell to cell and from time to time. (wikipedia.org)
  • Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)
  • We then developed a process to isolate single CTCs using laser capture microdissection for genome sequencing. (ubc.ca)
  • Here are the latest publications comparing PicoPLEX technology to other single-cell whole-genome amplification technologies, and using PicoPLEX technology to uncover insights from CTCs, single cells from FFPE tumor samples, and samples relevant to preimplantation genetic testing. (takarabio.com)
  • Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells. (takarabio.com)
  • This paper compared the performance of three commercially available whole genome amplification (WGA) technologies: the PicoPLEX WGA Kit , the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit. (takarabio.com)
  • Single and pooled tumor cells (SK-BR-3) obtained from EDTA- and CellSave-preserved blood and archival material were whole-genome amplified. (takarabio.com)
  • This paper compared the performance of the PicoPLEX DNA-seq Kit , the DOPlify WGA Kit, the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit for aneuploidy screening and copy number analysis using shallow whole genome sequencing, starting from one, three, or five cells isolated from the Loucy cell line. (takarabio.com)
  • Cells were whole-genome amplified, followed by PCR-free library preparation and Illumina sequencing. (takarabio.com)
  • This paper compared the performance of copy number variation (CNV) detection of four commercially available whole genome amplification (WGA) kits: the PicoPLEX WGA Kit , the GenomePlex Single Cell Whole Genome Amplification Kit, the MALBAC Single Cell Whole Genome Amplification Kit, and the REPLI-g Single Cell Kit. (takarabio.com)
  • Tumour-associated activating mutations in EGFR can identify patients with NSCLC who are likely to have a good response to TKIs. (bmj.com)
  • These results demonstrate the potential of our single cell sequencing workflow to discover clinically relevant mutations from single CTCs that may aid in monitoring disease progression and guiding treatment. (ubc.ca)
  • For example, loss-of-function mutations in Ptc1 , or gain-of-function mutations in Smo, Shh, Gli1 , and Gli2 , can each contribute to skin cancers, most notably basal cell carcinomas - the most common of all human cancers. (bcm.edu)
  • Somatic hypermutation may remain active in L&H cells in a considerable proportion of cases, increasing the risk of acquiring further transforming mutations. (bmj.com)
  • 1 Analysis of V gene rearrangements in single L&H cells revealed the presence of somatic mutations with intraclonal diversity in functional monoclonal V gene rearrangements, in line with a GC B cell origin. (bmj.com)
  • Germinal-center B-cells with unfavorable mutations are functionally crippled and undergo programmed cell death (apoptosis). (medscape.com)
  • With the improvements to their device, which uses a Velcro-like nanoscale technology, they can now detect and isolate single cancer cells from patient blood samples for analysis. (sciencedaily.com)
  • Kirsten rat sarcoma viral oncogene homolog ( KRAS ) mutation has been considered as a negative prognostic and predictive factor mainly in patients treated with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) ( 6 ). (iiarjournals.org)
  • HL-RSC carry clonal immunoglobulin gene rearrangements, which establishes their clonality and their B cell origin. (medscape.com)
  • Renal cell cancer (RCC) accounts for 2-3% of all tumor malignancies in human. (oncotarget.com)
  • The most frequently detected abnormalities were hyperechoic rings around renal pyramids (28.3% of patients). (medscape.com)
  • Flow cytometry was used to detect cell cycle and apoptosis. (besjournal.com)
  • Reduction of CYP1B1 levels fail to prevent in vitro tumorigenicity such as proliferation, apoptosis, and cell cycle progression of RCC cells. (oncotarget.com)
  • The centre of the tuberculous granuloma may undergo caseous necrosis surrounded by activated macrophages, T-cells and fibroblasts. (ersjournals.com)
  • Scientists of the Paul Langerhans Institute Dresden developed a new approach to isolate islets from snap frozen surgical specimens donated by metabolically phenotyped pancreatectomized patients. (tu-dresden.de)
  • Thus, it has become possible to apply these technologies to surgical specimens as experimental models to study beta cells in situ, and thereby in a context that more faithfully represent their state in vivo . (tu-dresden.de)
  • Although p16 INK4a has been well established as sensitive and accurate diagnostic biomarker for endocervical adenocarcinoma in situ and invasive adenocarcinoma in histologic sections and in cervical cytology specimens, clinical and diagnostic features alone have limited power to predict the survival of patients with endocervical adenocarcinoma. (medscape.com)
  • OBJECTIVES: To describe the morphologic characteristics of skin lesions, extent of extracutaneous disease, and outcomes in patients with neonatal presentation of Langerhans cell histiocytosis (LCH), and to examine clinical predictors of disease prognosis. (thedoctorsdoctor.com)
  • Conclusions We identified a somatic GJA4 mutation that presents in over one-third of ECH lesions and proposed that ECHs are vascular malformations due to GJA4 -induced activation of the SGK1 signalling pathway in brain endothelial cells. (bmj.com)
  • K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)
  • Objectives: To characterize phenotypes of T cells that accumulated in multiple sclerosis (MS) lesions, to compare the lesional T-cell receptor (TCR) repertoire of T-cell subsets to peripheral blood, and to identify paired α and β chains from single CD8+ T cells from an index patient who we followed for 18 years. (uni-muenchen.de)
  • Results: We found that a TCR Vβ1+ T-cell population that was strikingly expanded in active brain lesions at clinical onset comprises several subclones expressing distinct yet closely related Vα7.2+ α chains, including a canonical Vα7.2-Jα33 chain of mucosal-associated invariant T (MAIT) cells. (uni-muenchen.de)
  • Conclusions: Our observation that a massively expanded TCR Vβ1-Jβ2.3 chain paired with distinct yet closely related canonical or atypical MAIT cell-related α chains strongly points to an antigen-driven process in early active MS brain lesions. (uni-muenchen.de)
  • Conclusion Hypopigmented lesions of MF can be observed in Caucasian patients. (unipv.it)
  • This dissertation presents a new workflow to isolate, extract, and sequence single CTCs from patients with metastatic prostate cancer. (ubc.ca)
  • This workflow was used to successfully enumerate CTCs from 20 patients with metastatic castrate resistant prostate cancer, as well as 25 patients with localized prostate cancer. (ubc.ca)
  • Using this process, we enriched and isolated 30 single CTCs from 3 patients with metastatic prostate cancer, and sequenced 5 of these single CTCs from a patient with matched cell-free DNA. (ubc.ca)
  • The 5-year survival rate for patients with metastatic RCC is less than 10% probably due to its low response to chemo- and radiotherapy [ 2 ]. (oncotarget.com)
  • In 2008, an estimated 54,000 adults in the United States was diagnosed with RCC and approximately one third of newly diagnosed patients present with metastases [ 1 ]. (oncotarget.com)
  • In vitro studies (FRET and FLIM) also demonstrated that HNMT/HER2 complex formation in BC cells may affect therapeutic efficacy. (tmu.edu.tw)
  • Contemporary research of beta cell biology in context of type-2 diabetes is based mainly on the long-standing technique for isolation of the pancreatic islets of Langerhans. (tu-dresden.de)
  • Pancreatic endocrine islet β-cells create and release the two crucial hormones that regulate blood glucose levels: insulin, which acts to lower blood sugar, and glucagon, which raises blood sugar. (frontiersin.org)
  • Thus, the dysfunction of pancreatic islet β-cells is a significant cause of T2D. (frontiersin.org)
  • Conclusion: The presence of KRAS mutation (especially KRAS G12C mutation) correlated with adverse prognosis in patients treated with second- or third-line pemetrexed or docetaxel. (iiarjournals.org)
  • Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
  • Cell proliferation was measured by CCK8 and EdU assay. (besjournal.com)
  • CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner, and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine. (besjournal.com)
  • Interestingly, ULK2 inhibited CRC cell proliferation in a p53-dependent manner. (besjournal.com)
  • Furthermore, exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC cells. (besjournal.com)
  • Conclusion Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC, which may offer promising targets for CRC prevention and therapy. (besjournal.com)
  • Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood. (medscape.com)
  • Microdissection procedure: the upper part of the dermis (papillary dermis) is removed, along with the very lower part of the epidermis, with a 30-gauge needle under microscopic visual control (objective magnification ×40). (jamanetwork.com)
  • Considering the operative time and complication rates of bilateral MD-TESE in patients diagnosed with NOA and the possible MD-TESE procedures that may be performed later, we believe that unilateral MD TESE is a more preferable procedure for the patient and surgeon in this patient group. (springermedicine.com)
  • Patients treated with chemotherapy may develop complications in the eye, kidney, or hearing. (molvis.org)
  • The present study aimed at the elucidation of the role of KRAS mutation in prediction of outcome of patients with advanced NSCLC receiving second- or third-line chemotherapy. (iiarjournals.org)
  • One of the most important shifts leading to longer survival of patients with advanced-stage NSCLC was the introduction of increasing lines of chemotherapy. (iiarjournals.org)
  • Although monotherapy with docetaxel or pemetrexed has proven efficacy and safety in patients after failure of first-line platinum-based chemotherapy regimens in several randomized phase III clinical trials ( 3 , 4 ), the efficacy of second- or third-line chemotherapy seems to be relatively low ( 3 - 5 ). (iiarjournals.org)
  • First, it acts as a drug adjuvant to the chemotherapy, making it work more effectively against cancer cells. (concordia.ca)
  • Second, it appears to protect healthy cells from the toxicity of the chemotherapy. (concordia.ca)
  • Combined-modality therapy, including radiation and chemotherapy, is the preferred approach for most pediatric patients. (medscape.com)
  • Chemotherapy drugs can kill rapidly dividing cells by various mechanisms. (bestgrademiners.com)
  • Unfortunately, chemotherapy drugs can also kill other rapidly dividing cells in the body such as bone marrow cells, which means they have a number of side effects and sometimes leads to death. (bestgrademiners.com)
  • Chemotherapy drugs have many side effects arising from their impact upon fast-growing cells other than cancer cells. (bestgrademiners.com)
  • HER2+ Breast cancer (BC): HER2 overexpression in BC patients has been associated with poor response to hormone therapy and chemotherapy. (tmu.edu.tw)
  • Metastasis results from the dissemination of cancer cells from a primary tumour to other anatomical sites and is the most common cause of death for lung cancer patients. (ucl.ac.uk)
  • The majority of patients with non-small-cell lung cancer (NSCLC) present with advanced disease, with targeted therapies providing some improvement in clinical outcomes. (bmj.com)
  • Lung cancer is the most prevalent life-threatening cancer worldwide 1 with more than 80% being non-small-cell lung cancer (NSCLC). (bmj.com)
  • Background/Aim: The prognostic and predictive value of Kirsten rat sarcoma viral oncogene homolog (KRAS) mutation in non-small cell lung cancer (NSCLC) is not well established. (iiarjournals.org)
  • Non-small cell lung cancer (NSCLC) constitutes more than 80% of all lung carcinomas ( 2 ). (iiarjournals.org)
  • Recently, small cell lung cancer was shown to be dependent on activated hedgehog signaling. (bcm.edu)
  • The most highly infectious patients are those who develop cavitatory lung disease [ 1 ], since each cavity may contain up to 10 9 mycobacteria [ 5 ], and these patients can be regarded as the aerosol supershedders that drive the global pandemic. (ersjournals.com)
  • Approximately 85% of patients have a group of histological subtypes collectively known as Non-small cell lung cancer. (bestgrademiners.com)
  • Several investigators have hypothesized that endothelial cell (EC) abnormalities might contribute to this prothrombotic state. (uludag.edu.tr)
  • Results: Progression-free survival was not significantly different between patients with KRAS mutation and those with wild-type KRAS. (iiarjournals.org)
  • Overall survival was significantly longer for patients with wild-type KRAS vs. those with KRAS mutation (16.1 vs. 7.2 months, p=0,008). (iiarjournals.org)
  • Recent developments in technology may make the monitoring of changes in patients' tumour genotypes easier in clinical practice, which may enable patients' treatment regimens to be tailored during the course of their disease, potentially leading to improved patient outcomes. (bmj.com)
  • Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. (nature.com)
  • Histopathological-based models may be used to stratify patients into low- and high-risk classes 9 and further identify patients at risk of aggressive early-stage OSCC, thus contributing to disease predictability in terms of clinical progression and treatment outcomes. (nature.com)
  • Genomic analysis by transcriptome sequencing to predict the outcomes in HR patients. (tmu.edu.tw)
  • To analyse whether AID is expressed in L&H cells as an indicator of active somatic hypermutation in the tumour cells. (bmj.com)
  • The ability to capture and preserve single CMCs allows doctors to analyze melanoma cells' DNA structure, determine the genetic characteristics of the patient's cancer and confirm that the circulating cells remain genetically similar to the tumor they came from. (sciencedaily.com)
  • Ichioka K, Matsui Y, Terada N, Negoro H, Goto T, Ogawa O (2020) Three-dimensional simulation analysis of microdissection testicular sperm extraction for patients with non-obstructive azoospermia. (springermedicine.com)
  • Moreover, the device does not allow isolated cells to be washed from the supernatant prior to treatment or analysis. (justia.com)
  • Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
  • Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
  • Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)
  • The critical step of integrating all steps of single cell analysis into a robust system capable of performing measurements on large numbers of cells has yet to be reported. (justia.com)
  • The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. (geneticsmr.org)
  • With microdissection, we can isolate the tubules and conduct biochemical and molecular analysis. (concordia.ca)
  • The association with thyroid disease in the proband was explained partially by the involvement of the pituitary, with the relative risk decreasing when patients with diabetes insipidus and thyroid involvement were excluded from analysis. (thedoctorsdoctor.com)
  • When blood is passed through the chip, extremely small "hairs" -- nanoscale wires or fibers coated with protein antibodies that match proteins on the surface of cancer cells -- act like Velcro, traping CTCs and isolating them for analysis. (sciencedaily.com)
  • The preservation of single captured CMCs in this proof-of-concept study also allowed researchers to conduct an analysis -- called single-cell genotyping -- to find within the cell a specific target (BRAF V600E) for a drug called vemurafenib. (sciencedaily.com)
  • 3. Study the action of Herceptin-induced HNMT overexpression and the mechanisms that cause HR. Aim 3: Establish HNMT inhibition (siRNA) and overexpression cell lines to clarify the HR-related signaling pathways Year-2 proposal: Study the mechanisms of HNMT-mediated gene regulation in the HR cells Aim 1: Establish the HR cells for transcriptome sequencing analysis 1. (tmu.edu.tw)
  • 2. RNA-quantification (or transcriptome) sequencing analysis in HR cells during different Herceptin® training-passages (p1〜p12). (tmu.edu.tw)
  • Observation of HNMT/HER2 complex formation in live cell models by Split Luciferase, FRET, and FLIM analysis to prove its association with HR. 2. (tmu.edu.tw)
  • 2. Transcriptome sequencing analysis for these primary cultured HR cell lines. (tmu.edu.tw)
  • 2. The HR-cell xenografted tumor animals will be used for FRET or Split Luciferase analysis. (tmu.edu.tw)
  • Veelken HWood GSSklar J Molecular staging of cutaneous T-cell lymphoma: evidence for systemic involvement in early disease. (jamanetwork.com)
  • Background: Hypopigmented mycosis fungoides (MF) is a rare variant of cutaneous T-cell lymphoma, it is more frequent in dark-skinned or Asian patients, particularly children. (unipv.it)
  • T, p.G41C) in 5/12 patients with ECH in the discovery cohort and confirmed the finding in the validation cohort (16/46). (bmj.com)
  • In adenocarcinomas, K17 staining was detected in a mean of 33.9% of malignant cells. (medscape.com)
  • Although this surgery is the best way to save these children's lives, it can be a stressful experience for pediatric patients and their families. (molvis.org)
  • Single-nucleotide polymorphisms in the LRWD1 gene may be a genetic risk factor for Japanese patients with Sertoli cell-only syndrome. (medscape.com)
  • Muscle biopsy and genetic testing are the most important tools used in the diagnostic evaluation of patients in whom limb-girdle muscular dystrophy (LGMD) is suspected. (medscape.com)
  • Firstly, we compared patient survival [progression-free (PFS) and overall (OS)] according to KRAS gene status. (iiarjournals.org)
  • Continuous understanding on the mechanisms underlying the pathogenesis of this disease and identification of biomarkers for therapeutic application and response to treatment will help to improve patient survival. (bvsalud.org)
  • K17 is highly expressed in most cases of both invasive adenocarcinoma and in AIS and is a powerful, negative prognostic marker for patient survival. (medscape.com)
  • A key challenge in CTC isolation and characterization has been their extreme rarity in blood and their cell-to-cell heterogeneity. (ubc.ca)
  • CTCs trapped by the chip also act as a "liquid biopsy" of the tumor, providing convenient access to tumor cells and earlier information about potentially fatal metastases. (sciencedaily.com)
  • The lymphocytic and histiocytic (L&H) cells of lymphocyte predominant Hodgkin lymphoma (HL) originate from germinal centre B cells and carry mutated V gene rearrangements, usually with intraclonal diversity. (bmj.com)
  • Mixed cellularity Hodgkin lymphoma showing both mononucleate and binucleate Reed-Sternberg cells in a background of inflammatory cells (hematoxylin and eosin, original magnification X200). (medscape.com)
  • Hodgkin lymphoma (HL) is a malignancy of the germinal-center B cells that affects the reticuloendothelial and lymphatic systems. (medscape.com)
  • Tseng's team now reports that they have improved the NanoVelcro chip by replacing its original non-transparent silicon nanowire substrate inside with a new type of transparent polymer nanofiber-deposited substrate, allowing the device's nanowires to better "grab" cancer cells as blood passes by them. (sciencedaily.com)
  • We now can not only capture cancer cells from blood with high efficiency but also hand-pick single CTCs for in-depth characterization to provide crucial information that helps doctors make better decisions. (sciencedaily.com)
  • If cancer cells are in the body long enough, they can grow/invade into nearby areas. (bestgrademiners.com)
  • Cancer cells also work together. (bestgrademiners.com)
  • Thus, the identification of complementary biological signatures that assist in the prognostic prediction of patients with OSCC is needed. (nature.com)
  • [ 14 ] Subsequent work in our laboratory demonstrated that K17 serves as a nuclear shuttle of p27KIP1, resulting in sustained cell cycle progression. (medscape.com)
  • Bernie AM, Ramasamy R, Schlegel PN (2013) Predictive factors of successful microdissection testicular sperm extraction. (springermedicine.com)
  • Tournaye H, Verheyen G, Nagy P, Ubaldi F, Goossens A, Silber S et al (1997) Are there any predictive factors for successful testicular sperm recovery in azoospermic patients? (springermedicine.com)
  • Pan LXDiss TCPeng HZNorton AJIsaacson PG Nodular lymphocyte predominance Hodgkin's disease: a monoclonal or polyclonal B-cell disorder? (jamanetwork.com)
  • Since CTCs are accessible from peripheral blood, these cells represent a potential source of highly relevant tumor materials, which could be used to reveal new biomarkers for monitoring disease progression and evaluating drug efficacy. (ubc.ca)
  • Initially, we investigated the morphology of CTCs from patients with prostate cancer, and observed that CTCs and leukocytes were similar in size, but distinct in nucleus-to-cytoplasm ratio, which suggests the potential to separate CTCs based on deformability. (ubc.ca)
  • Based on this result, a microfluidic device that separates CTCs based on cell deformability, as well as an accompanying analytical pipeline to identify CTCs using immunofluorescence, were developed, optimized, and tested. (ubc.ca)
  • We retrospectively analyzed clinical data of 129 patients with cytologically- or histologically-confirmed advanced-stage (stage IIIB or IV) NSCLC treated with docetaxel or pemetrexed in second or third line between 2006 and 2015 at the Department of Pneumology University Hospital in Pilsen. (iiarjournals.org)
  • Overexpression of DUSP10 in NSCLC cells resulted in reduced activation of ERK and JNK, but increased activation of p38, which was associated with increased cellular growth and migration. (bvsalud.org)
  • Using the new assay on patients' blood containing circulating melanoma cells (CMCs), Tseng's team was able to isolate and preserve single CMCs. (sciencedaily.com)
  • The group was successfully able to show the feasibility and safety of magnetic resonance imaging (MRI) - controlled transurethral ultrasound therapy for prostate cancer in eight patients. (pharmaceuticalintelligence.com)
  • High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
  • Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)
  • In particular, microfluidic qPCR devices (Biomark Dynamic Array, Fluidigm) have recently been applied to single cell studies (15, 16). (justia.com)
  • Deformability-based separation and single-cell sequencing. (ubc.ca)
  • We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
  • These results suggest that CYP1B1 up-regulation mediated by low miR-200c is one of the mechanisms underlying resistance of RCC cells to docetaxel. (oncotarget.com)
  • Aim 2: Study the possible mechanisms of HNMT-mediated HR in BC cells 1. (tmu.edu.tw)
  • Year-3 proposal: Animal studies on the mechanisms of HNMT-mediated gene regulation in the HR cells Aim 1: Establish the HNMT-HR cell-xenografted tumor model 1. (tmu.edu.tw)
  • Hypopigmented mycosis fungoides in Caucasian patients: a clinicopathologic study of 7 cases. (unipv.it)
  • When these cells are isolated from the blood early on, they can provide doctors with critical information about the type of cancer a patient has, the characteristics of the individual cancer and the potential progression of the disease. (sciencedaily.com)
  • It is vital to establish accurate tools to identify those patients at highest risk for disease severity or progression in order to determine who are in need of immediate therapies. (biomedcentral.com)