Patient s advantages cells cultured. Medical search. Frequent questions

Epithelial cells and fibroblasts are examples of these types. (microbiologynote.com)
These stem cells can be either isolated from tissue biopsies or generated based on hair follicle cells or skin fibroblasts. (eceae.org)
Induced pluripotent stem cells (iPSCs) generated from patient fibroblasts could potentially be used as a source of autologous cells for transplantation in retinal disease. (nature.com)
Through established protocols 2 , fibroblasts from a skin biopsy can be returned to a pluripotent state and serve as a renewable, autologous source of replacement cells that avoids the ethical complications of hESCs. (nature.com)
To generate iPSCs, dermal fibroblasts were cultured from the skin biopsy sample and transduced according to previously described protocols 5 . (nature.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

However, once feasibility is established and you decide to move into the next phase of the development process to produce larger quantities for analysis or pre-clinical material, it's worth considering whether to keep your process in adherent cells or look at moving to a suspension platform. (pall.com)
another monoxenous trypanosomatid, among clinical isolates from VL patients in India: of 120 cultured isolates, 111 were typed as L. donovani and 9 as Leptomonas sp. (cdc.gov)
parasites in clinical isolates of Kala-azar patients from India. (cdc.gov)
The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
At the conclusion of today's session the participant will be able to describe the current status of influenza activity in the United States, discuss the circulating influenza strains seen this season and the implications for clinicians, discuss the use of influenza diagnostic tests and the role in clinical care and discuss anti-viral treatment implications for patients evaluation treatment and testing. (cdc.gov)
Our senior staff of stem cells procedures is lead by Dr. Wu Li Ke and Dr. Wang Xiao Juan both have years of extensive stem cell research experience conducting clinical trials in China - Hebei Medical University. (mediescapes.com)
In some immunocompromised patients, who shed the virus for long periods, the diagnosis can be of little clinical significance. (medscape.com)
This study shows that we can stifle the growth of invasive brain tumors with a compound that has a substantial clinical advantage, and can aid in the reduction or refinement of mainstream treatments, particularly radiation and/or chemo," said Lohitash Karumbaiah, associate professor of regenerative medicine in UGA's College of Agricultural and Environmental Sciences. (sciencedaily.com)
In recent years, the development in cell therapy and stem cell research has led scientist to engage in the production of cells and tissue product that is of clinical grade. (biomedcentral.com)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Browse through our list of patient and caregiver resources to help you throughout your cancer journey, and learn more about clinical trials. (standuptocancer.org)
Due to pre-clinical experiments, animal models replicate some lesions such as damage in retinal ganglion cell (RGC) axons, some changes in the anterior segment, death of RGC, alterations due to ocular hypertension (OH), and some alterations in the head of the optic nerve (ON) and the lamina cribrosa (LC) and being cost-effective during practical. (credihealth.com)
To put these model systems into a clinical perspective, also advantages and limitations of patient studies will be discussed. (universiteitleiden.nl)
Recent clinical trials evaluating allogeneic retinal grafts derived from human embryonic stem cells (hESCs) show the procedure to be safe and potentially effective 1 . (nature.com)
October 19, 2010 (Washington, DC) - An online wound (OW) electronic medical record (EMR) can contribute significantly to a decrease in amputation rates in patients with diabetes and lower extremity wounds, according to a study presented here at the American College of Surgeons 96th Annual Clinical Congress. (medscape.com)
RIDTs may be used to help with diagnostic and treatment decisions for patients in clinical settings, such as whether to prescribe antiviral medications. (cdc.gov)
PCR assay or culture isolation and proved suitable to probe hydrolysis technology (Applied Biosystems, Foster detect SARS-CoV in clinical specimens. (cdc.gov)
A total of 340 clinical specimens collected from 246 were quickly developed: tissue culture isolation, antibody persons with confirmed or suspected SARS-CoV infec- detection, and reverse transcription-polymerase chain tion (13) were used in this study. (cdc.gov)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)

Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Furthermore, linking genotyping information of cultivated (cloned) strains with a patient phenotype is risky because of the selection biases of in vitro isolation and maintenance. (cdc.gov)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
a multi-institutional team lead by Dr. Daniel G. Anderson of the Massachusetts Institute of Technology and Boston Children's Hospital describes a new approach in which β cells differentiated in vitro from human pluripotent stem cells (hPSCs) were packaged in a modified alginate matrix and transplanted into immuno-competent C57BL/6J mice ( 000664 ) made diabetic by treatment with streptozotocin (STZ), which selectively destroys the mouse b cells. (jax.org)
First, they developed an in vitro culture method to create human insulin-producing β cells from huPSCs (stem cell-derived β cells, SC-β). (jax.org)
The paper was spearheaded by two senior scientists at Crown Bioscience, Dr Rajendra Kumari, Executive Director, Integrated Solutions and Dr Xiaoxi Xu, Senior Director, Molecular and Cellular Biology, and details how disruptive organoid technology was deployed to create matched 3D in vitro and in vivo patient-relevant systems. (businesswire.com)
Dr Rajendra Kumari, Executive Director, Integrated Solutions at Crown Bioscience said: "There has been a significant increase in demand in the last five years in generating in vitro and in vivo matched patient models in preclinical oncology research. (businesswire.com)
This review paper offers essential insights into why 3D in vitro organoids are key culture systems for selecting successful drug candidates, their differences and advantages over other systems, like 2D cell cultures and 3D spheroids, and their applications in drug discovery. (businesswire.com)
Organoids derived from patients suffering from a genetic disorder conserve the genetic mutations in vitro. (eceae.org)
Organoids generated from these patients did thus not display cAMP-induced swelling in vitro, genetic correction of the mutation in the organoids however restored this response (4). (eceae.org)
As linear correlations exist between expression levels of angiogenic factors under normoxic and hypoxic conditions in vitro , we propose that explanted primary cells may be used to probe the in vivo hypoxic environment. (biomedcentral.com)
Further studies should be performed to correlate in vitro protein expression levels of angiogenesis-related factors with in vivo patient response. (biomedcentral.com)
In vitro studies have shown that stromal cells cultured in hypoxic growth conditions secrete higher levels of critical angiogenesis-inducing factors than cells cultured in normoxic conditions [ 3 ]. (biomedcentral.com)

Coupling with stem cell research, researchers have successfully grown simple organs derived from autologous human pluripotent stem cells. (wikipedia.org)
The aim of this paper is to discuss the general overview of the quality assurance and quality control implemented in the manufacturing of cell and tissue product, with emphasis on our experience in the manufacturing of MyDerm ® , an autologous bilayered human skin substitute. (biomedcentral.com)
MACI (autologous cultured chondrocytes on porcine collagen membrane). (tocshreveport.com)

The association between prognosis and islet macrophage count has been confirmed recently by others in an independent cohort of patients 5 but the stromal macrophage counts in that study were not associated with survival. (ersjournals.com)
However, a major limitation of this emerging technology is the absence of a tumor microenvironment that includes immune and stromal cells . (bvsalud.org)

The tissue must be placed and harvested in an aseptic environment such as sterile laminar flow tissue culture hood. (wikipedia.org)
Tissue Culture is the term used to describe the process of removing tissues, cells or organs from either a plant or animal and their subsequent transfer into an artificial environment that encourages development. (microbiologynote.com)
Transfer two milliliters of the liquid culture medium into each well of a 24 well tissue culture plate. (jove.com)
Take two colonies with an inoculating needle from the inoculated petri dishes in step, and place them into the first well of the tissue culture plate. (jove.com)
Incubate the tissue culture plate at 30 degrees Celsius for seven days. (jove.com)
Ever since, the accelerating field of human three-dimensional tissue culture has opened new avenues for biomedical applications and has given rise to a multitude of ground-breaking approaches such as organ-on-a-chip systems and personalised medicine. (eceae.org)
Patients had multiple pathogens on deep tissue culture, and only 3 did not have evidence of osteomyelitis or peripheral arterial disease . (medscape.com)

Industry leaders exchanged ideas about getting started in 3D, workflow optimizations, and the future of 3D cell culture applications, from spheroids and organoids to tissue models. (corning.com)
One presentation at the summit underscored the potential of human pluripotent stem-cell-derived organoids to help address viral diseases such as COVID-19 through disease modeling and drug screening. (corning.com)
After developing a panel of hPSC-derived cells and organoids and screening ten different types, Dr. Chen and team found that lung, colon, heart, liver, and pancreatic cells could be infected by the novel coronavirus. (corning.com)
The paper is the first comprehensive review of tumor organoids derived from Patient Derived Xenograft (PDX) models across multiple cancer types. (businesswire.com)
Developed from adult stem cells residing in the tumor, the paper describes the establishment of living biobanks of organoids readily available for drug discovery and development research. (businesswire.com)
Organoids are organ-like structures that are grown in the laboratory from human-derived stem cells. (eceae.org)
The organoids are three-dimensional organ-like structures harbouring multiple cell types that are organized in a physiological architecture. (eceae.org)
Accordingly, organoids are grown from healthy donors or from patients suffering from a certain disease (1). (eceae.org)
Mini brains are generated from patients suffering from Alzheimer's or Parkinson's disease, pancreatic organoids from diabetic patients, intestinal organoids from people suffering from Chron's or inflammatory bowel disease, 3D lung models are generated from smokers and COPD (chronic obstructive pulmonary disease) or asthma patients. (eceae.org)
Brain organoids derived from human iPSCs can develop brain tumours (2), e.g. by adding human brain cancer cells (3). (eceae.org)
Personalised medicine is already available for cancer patients: organoids are grown from tumour biopsies and drugs are tested on these so-called microtumours in order to identify the most effective therapy which is finally given to the patient (5,6). (eceae.org)
Extensive validation studies have confirmed the versatility of this hydrogel with a wide range of cell types, successfully demonstrating the reproducible induction of organoids from various organ types, including kidney, liver, intestine, and many more. (labx.com)
Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids Derived from Patients. (bvsalud.org)
The three-dimensional tumor organoids derived from self -organizing cancer stem cells are valuable ex vivo models that faithfully replicate the structure, unique features, and genetic characteristics of tumors . (bvsalud.org)
To address this limitation, co-culture techniques combining tumor organoids and T cells have been developed, offering diverse avenues for studying individualized drug responsiveness. (bvsalud.org)
Recent progress in co-culture models of tumor organoids has allowed for a better understanding of the advantages and limitations of this novel model, thereby exploring its full potential. (bvsalud.org)

Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
According to their source primary cells develop either in a monolayer that is adherent or as a suspension. (microbiologynote.com)
The base of the vessel is covered by the same cell layer, typically only one cell thick is known as monolayer culture. (microbiologynote.com)

Another case was from a team at University College, London, UK, which transplanted a wind pipe derived from the patient's own stem cells. (wikipedia.org)
In autoimmune type 1 diabetes (T1D), a patient's own T cells destroy the insulin-producing β cells in the pancreas, leading to progressively worse hyperglycemia. (jax.org)
Several surgeons create total knee implants specific to each patient, created by 3D printing based on the patient's MRI. (tocshreveport.com)
With this procedure, a biopsy is collected and the patient's cells are collected and expanded on a special membrane which is then implanted in the patient's knee. (tocshreveport.com)
Using a patient's own cells for transplantation would avoid these pitfalls and is possible with induced pluripotent stem cells (iPSCs). (nature.com)
email alerts and Blackberries, of any change in a patient's status - [from] radiology reports, culture reports, and vascular testing. (medscape.com)

Over time, progenitor cells migrate out of the tissue onto the surface of the dish. (wikipedia.org)
The hiPSCs were differentiated into neural progenitor cells for two different layers of the cerebral cortex, by using specific combinations of growth factors and chemicals. (ox.ac.uk)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

2023) Updating cancer research with patient-focused networks. (news-medical.net)
At the end of 2023, researchers at Lund Stem Cell Center received funding amounting to SEK 77.5 million from the Swedish Research Council, the Swedish Cancer Society, and the Swedish Childhood Cancer Fund. (lu.se)

A research team from Wake Forest Institute for Regenerative Medicine in Winston-Salem, North Carolina, successfully transplanted stem cell-engineered bladders to seven pediatric patients with malfunctioning bladders. (wikipedia.org)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

They are also a suitable cellular substrate for the generation of induced pluripotent stem cells (iPSCs) via nuclear reprogramming, offering a number of advantages over other cell types. (uea.ac.uk)
Unlike embryonic stem cells, iPSCs are derived from adult human cells. (jax.org)
iPSCs "can be turned into disease-relevant cells such as nerve cells, allowing researchers to study disease biology in the very types of cells that become affected. (jax.org)
However, the process of making iPSCs from patients with these diseases, and the subsequent genetic engineering of the cell lines, is "difficult, expensive and time consuming," says Ward. (jax.org)
Patient-derived iPSCs, however, would still harbor disease-causing mutations. (nature.com)
We tested whether CRISPR/Cas9 could be used in patient-specific iPSCs to precisely repair an RPGR point mutation that causes X-linked retinitis pigmentosa (XLRP). (nature.com)
This is the first report using CRISPR to correct a pathogenic mutation in iPSCs derived from a patient with photoreceptor degeneration. (nature.com)
Although the original disease-causing mutation would still be present in patient iPSCs, precise mutation correction is possible through gene editing techniques adapted from the bacterial clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system 3 . (nature.com)
In this study, we generated iPSCs from a patient with a mutation in the retinitis pigmentosa GTPase regulator ( RPGR ) gene, which causes an aggressive, X-linked variant of RP (XLRP). (nature.com)

Which Production Method is Right for Your Cell Culture Process - Adherent or Suspension? (pall.com)
New gene therapies frequently start their development journey being produced in adherent cell lines, usually for good reasons. (pall.com)
It's no wonder that many academic and research facilities prefer adherent cells. (pall.com)
While the current adherent bioreactors on the market can provide up to 500 m 2 of surface area for cell growth, this still may not be sufficient to produce the titers needed for gene therapies requiring a high dose per patient, or for indications where the target population is high. (pall.com)
A suspension process has the advantage of being easier to operate than an adherent one. (pall.com)
Adherent processes require cells to be dissasociated from their growth surface for routine maintenance, scale-up and counting. (pall.com)
If you do notice a decrease in yields, then you may need to consider adapting the adherent cell line where better productivity was observed. (pall.com)
Here, we present a simplified metabolite extraction (SiMeEx) method, to efficiently and quickly extract metabolites from adherent mammalian cells. (frontiersin.org)
For adherent mammalian cells in culture the normal procedure involves their detachment from the surface. (frontiersin.org)
In this current study, we present a new and shorter method, termed as simplified metabolite extraction (SiMeEx), to extract metabolites from adherent mammalian cells. (frontiersin.org)
Scaling up viral vector production using adherent cell culture systems is challenging. (sigmaaldrich.com)
They are also known as anchorage independent cells or non-adherent ones which can be developed floating in the medium of culture. (microbiologynote.com)

They are derived from mesenchymal stem cells cultured in a lab. (skincarephysiciansofga.com)

Gene therapies in the market today use viral vectors to introduce modified genetic material into targeted cells. (sigmaaldrich.com)
Metastatic melanoma is one of the most difficult cancers to cure and recent advances in the development of targeted therapies have improved patient outcome. (edu.au)
It is a pioneering collection in the world, created to accelerate the search for therapies against brain metastasis, a disease that affects up to 30% of patients with systemic cancer. (news-medical.net)
Beyond their value to understanding endothelial biology and disease modeling, BOECs have potential uses in endothelial cell transplantation therapies. (uea.ac.uk)
Tissue regenerative therapies, especially those in which patients are given implants derived from their own stem cells, could be a promising route to treat brain injuries in the future. (ox.ac.uk)
For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
The SU2C IRG program is one of two initial funding models created by SU2C to focus on groundbreaking translational research aimed at getting new therapies to patients quickly. (standuptocancer.org)

2014). Self-Organizing Properties of Mouse Pluripotent Cells Initiate Morphogenesis upon Implantation. (wikipedia.org)
The latter approach has the advantage of being non-invasive to the human donor as the mature cells are reprogrammed by genetic engineering yielding induced pluripotent stem cells (iPSC). (eceae.org)
The cortical structure was made from human induced pluripotent stem cells (hiPSCs), which have the potential to produce the cell types found in most human tissues. (ox.ac.uk)
Thanks to human induced pluripotent stem cell (iPSC) and gene-editing technologies, it's possible to derive every kind of brain cell type, insert dementia -related genes and study them in culture. (jax.org)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
The majority of cell lines that are continuous are developed in monolayers. (microbiologynote.com)

Monoclonals can be used directly to bind to surface proteins on cancer cells to kill or inhibit their growth," explains Dr. Moran. (labmanager.com)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
The samples are often minced, and the pieces are placed in a cell culture dish containing growth media. (wikipedia.org)
These transgenic labels can help researchers observe growth of specific cells. (wikipedia.org)
Once your cells are no longer dependent on a surface for growth, scale-up becomes much easier and the range of technology to support manufacture increases. (pall.com)
A move to a different cell line or adapting an existing one is also a good opportunity to change the production medium, whether it's to go fully chemically-defined or boost cell growth. (pall.com)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
Assuming that sterility was preserved during the experiment, we interpreted that original infection in patients resulted from a mixture of the 2 species, Leptomonas overgrew L. donovani in culture because of substantial growth advantage of the former, and a few remaining L. donovani cells in the culture spread in the animals after inoculation because of their higher in vivo fitness. (cdc.gov)
This technique appears to improve embryo growth for patients who have had poor embryo development and unsuccessful IVF cycles. (ucsfhealth.org)
Sf9 Rhabdovirus-Negative (Sf-RVN ® ) Platform provides a rhabdovirus-free cell line that improves the safety profile of your bioprocesses combined with a chemically defined medium specifically formulated to get excellent growth and productivity while adhering to regulatory requirements. (sigmaaldrich.com)
The environment is usually comprised of the right glass or plastic container for the culture that contains a semisolid or liquid medium that provides the necessary nutrients for growth and survival. (microbiologynote.com)
The study of the whole organ or organ fragments that are intact to study their ongoing function or their growth is termed Organ Culture. (microbiologynote.com)
Specific growth factors added to the culture medium determine the fate of the stem cells shifting the development towards the formation of a kidney, intestine, brain or liver organoid for instance. (eceae.org)
1 Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. (jci.org)
The UGA researchers identified that surfen-treated cells were "blocked" from tumor growth, and the spread of tumor cells in the brain. (sciencedaily.com)
Designed to mimic the neural pathways of the brain, the device allows for real-time monitoring of tumor cell adhesion and growth. (sciencedaily.com)
Growth competition experiments were carried out at equal multiplicity of infection in activated T cells and monocyte-derived dendritic cells (MO-DC) in parallel. (uantwerpen.be)

As part of the conversation, speakers also discussed the advantages and challenges of organoid models, including model validations. (corning.com)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
MatriMix overcomes the limitations of current 3D substrates, enabling cutting-edge applications such as organoid formation using adult patient-derived cells and iPSC culture. (labx.com)
By integrating cellular components of the tumor microenvironment , including T cells , into tumor organoid cultures , immuno-oncology has embraced this technology , which is rapidly advancing. (bvsalud.org)
This review focuses on the current applications of organoid - T cell co-culture models in cancer research and highlights the remaining challenges that need to be addressed for its broader implementation in anti- cancer therapy . (bvsalud.org)

Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)

This means gene therapy manufacturing starts with the upstream production of vectors either through transient transfection or use of stable producer cell line which culminates in the harvesting of the viral vector produced. (sigmaaldrich.com)
See how the Sf9 rhabdovirus-free cell line was developed and how we've developed a companion chemically-defined insect cell media for protein and viral expression. (sigmaaldrich.com)
Viral cultures are still the criterion standard for most viral pathogens, but they take a long time to complete. (medscape.com)
Viral-antigen detection is one of the new tests, but the results are generally less sensitive and less specific than those of conventional cell cultures. (medscape.com)
Because of the difficulty in distinguishing between the various etiologic agents, both viral and bacterial, causing pneumonia, the workup for symptomatic patients with an infiltrate on chest radiograph should include laboratory studies. (medscape.com)
Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)
Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)
PCR) or viral culture. (cdc.gov)

Next, they introduced live rodent models with cells that could grow into invasive tumors. (sciencedaily.com)
The therapeutic efficacy of immune checkpoint inhibitors has underscored the importance of immune cells , particularly cytotoxic T cells that infiltrate the vicinity of tumors , in patient prognosis . (bvsalud.org)
Another recent study with patients with hematological malignancies and head and neck solid tumors revealed that the majority of cases of oral candidiasis were caused by C. albicans, but almost one third of patients harbored non-albicans strains, such as C. glabrata, which were often more resistant to anti-fungal agents 7 . (bvsalud.org)

The VirusExpress ® 293T Lentiviral Production Platform offers a transfection-based solution to lentiviral (LV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and process with proven performance at 50 L scale. (sigmaaldrich.com)
The VirusExpress ® 293 AAV Production Platform offers a transfection-based solution using a suspension adapted cell line, chemically defined medium, and a clinically relevant process dramatically reducing time to production. (sigmaaldrich.com)
Third, using these cell lines, scientists were able to develop standardized, chemically defined culture media that made it far easier to grow cells. (microbiologynote.com)

While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
CMV infection characteristically is associated with "owl's-eye" cells, which are large cells with basophilic intranuclear inclusions and a surrounding clear zone. (medscape.com)
As a result of this high drug concentration, healthy cells may be killed as well as cancer cells, leaving many patients weak, nauseated and vulnerable to infection. (cam.ac.uk)
Through education, screening, partner referral, and proper patient care, public health workers and health-care practitioners can combine efforts to decrease the morbidity and costs resulting from this infection. (cdc.gov)
The prevalence of chlamydial infection also has been higher among those patients who live in inner cities, have a lower socioeconomic status, or are black (5-11). (cdc.gov)
Results: Dual infection/competition experiments in activated T cells clearly indicated that CRF02_AG isolates had a significant replication advantage over the subtype A and subtype G viruses. (uantwerpen.be)
In these patients, an important infection described as Candida-associated denture stomatitis (DS) occurs in about 50-60% of them 5 . (bvsalud.org)
However, due to th e limited sensitivities and predictive values of RIDTs, negative results of RIDTs do not exclude influenza virus infection in patients with signs and symptoms suggestive of influenza. (cdc.gov)
Acanthamoeba infection of the CNS occurs almost entirely in immunocompromised or otherwise debilitated patients, but B. mandrillaris may also infect healthy hosts. (msdmanuals.com)
In a few patients with AIDS, disseminated Acanthamoeba infection has affected only the skin. (msdmanuals.com)
In patients with B. mandrillaris infection of the brain, CT and MRI, both with contrast, typically show multiple nodular, ring-enhancing lesions. (msdmanuals.com)

Animal Cell Culture Types, Application, Advantages And Disadvantages. (microbiologynote.com)
For example, several models of glaucoma, such as animal models, cell cultures, and post-mortem eyes, have been studied with many advantages and disadvantages. (credihealth.com)

The role of a specific gene, gene expression, and the mechanism of action all can be studied with explant culture as well. (wikipedia.org)
Draw on our experience to learn how you can speed your gene therapy to patients. (sigmaaldrich.com)
The second approach is to use either the new gene editing CRISPR/Cas9 technology or mass spectrometric/proteomics approaches (or both) to undertake screens to identify factors that can inhibit the cell death that occurs as a consequence of disarming the autophagy pathway through deletion of Beclin. (edu.au)
This approach (i) allows for the generation of patient-specific endothelial cells from a relatively small volume of adult peripheral blood and (ii) produces cells that are highly similar to primary endothelial cells in morphology, cell signaling and gene expression. (uea.ac.uk)
Turboknockout , which expedites your ophthalmic research , is a cutting-edge technology that is more advanced than ES cell-mediated gene targeting. (credihealth.com)
To generate healthy patient-derived cells, mutations might be repaired with new gene-editing technology based on the bacterial system of clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9, thereby yielding grafts that require no patient immunosuppression. (nature.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

The key advantage of these cells is that large numbers can be created in culture for transplantation. (jax.org)
Among all, cell transplantation therapy is considered the most favourable treatment strategy for femoral head necrosis. (ijpsonline.com)
Stem cell-derived cell transplantation in the eye is one therapy being explored for inherited retinal degenerations such as retinitis pigmentosa (RP). (nature.com)

In a separate experiment, SC-β cell-loaded unmodified and TMTD-modified alginate microspheres were isolated from engrafted mice 14 days post-engraftment, and the presence of host immune cells adhering to the microspheres was examined by flow cytometry. (jax.org)
Hence, compared to the cell cultures experiment, the animal models present more similar physiological responses in glaucoma patients. (credihealth.com)

Similar to other RNA viruses, HEV builds a so-called mutant cloud, which represents an intra-host heterogeneous population, with the advantage of rapid adaptation to environmental conditions 19 . (nature.com)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
The cells in this culture are mostly heterogeneous cells. (microbiologynote.com)

4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. (wikipedia.org)
We use a combination of biochemical, cell biology, structural biology and medicinal chemistry approaches to understand the precise molecular mechanisms that control apoptosis. (edu.au)

The sensitivity to these reagents will be assessed in cell culture systems as well as in mouse models. (edu.au)
Research contracts have been already signed to exploit patient-derived organotypic cultures (PDOCs) as avatars, thus providing the possibility to generate biomarkers of sensitivity or resistance to specific drugs' the authors explain in Trends in Cancer . (news-medical.net)
These tests have sensitivity better than that of cultures. (medscape.com)
CHROMagar Candida™ medium (CC) is a differential culture medium that allows the presumptive identification of yeasts species, with sensitivity and specificity higher than 99% to C. albicans 10 . (bvsalud.org)
if detected, amebas may be cultured and tested for drug sensitivity. (msdmanuals.com)

The major advantage of explant culture is the maintenance of near in vivo environment in the laboratory for a short duration of time. (wikipedia.org)
The composition of extracellular matrix, for example, must be precisely similar to that of in vivo conditions in order to induce naturally observed behaviors of cells. (wikipedia.org)
Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, or blastocyst implant culture. (wikipedia.org)
Crucially, it will also empower drug developers to make confident decisions early with patient-derived models before progressing to in vivo studies, reducing, refining and replacing (the 3 Rs) the use of animals where possible. (businesswire.com)
MatriMix's ability to facilitate excellent cell organization and accurately replicate in vivo conditions makes it the ultimate solution for culturing difficult to grow cell types and drug screening. (labx.com)
Conclusion: We observed a higher ex vivo replicative fitness of CRF02_AG isolates compared to subtype A and G viruses from the same geographic region and showed that this was independent of the co-receptor tropism and irrespective of high or low CD4+T cell count. (uantwerpen.be)

We describe a method for the reliable generation, culture and characterization of BOECs from adult peripheral blood for use in these and other applications. (uea.ac.uk)

While each tissue is cultured in its own optimized environment, the whole is integrated through a recirculating vascular flow with monocytes by a matured and selectively permeable endothelial barrier. (corning.com)
Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular mechanisms underlying endothelial dysfunction in these individuals. (uea.ac.uk)
However, the recent identification of blood outgrowth endothelial cells (BOECs), generated from circulating endothelial progenitors in adult peripheral blood, may circumvent this limitation by offering an endotheliallike, primary cell surrogate for patient-derived endothelial cells. (uea.ac.uk)

If impossible, results based on cultured isolates should be interpreted with caution. (cdc.gov)
Of interest, patient cell isolates differed in the precise pattern of elevated cytokines. (biomedcentral.com)
In MO-DC cultures, CRF02_AG isolates showed a slightly but not significantly higher replication advantage compared to subtype A or G isolates. (uantwerpen.be)

Perform diagnostic testing on symptomatic patients with suspected pulmonary embolism to confirm or exclude the diagnosis or until an alternative diagnosis is found. (medscape.com)

In addition to standard IVF procedures - including intracytoplastmic sperm injection , assisted hatching, and blastocyst culturing - we offer a more unique technique, called embryo co-culture. (ucsfhealth.org)
Blastocyst culturing is a technique to grow embryos beyond the third day of culture. (ucsfhealth.org)
In certain patients, blastocyst culturing allows optimal selection of embryos for transfer, resulting in an increased implantation rate per embryo transferred. (ucsfhealth.org)
For younger patients, up to 50 percent of all embryos will continue to grow to the blastocyst stage. (ucsfhealth.org)
However, 10 percent of patients won't have an opportunity for embryo transfer due to the absence of blastocyst development. (ucsfhealth.org)
As patients get older, fewer and fewer numbers of embryos are capable of developing in culture to the blastocyst stage. (ucsfhealth.org)

Researchers and clinicians have long sought a method to replace the β cells, but these efforts have been hindered by the difficulty in developing a reliable and sustainable donor b cell source and a way to engraft them that avoids both immune-mediated allogeneic rejection and continuing autoimmune b cell destruction. (jax.org)
Researchers have attempted encapsulating donor β cells in a variety of materials to prevent allo- and xenogeneic rejection, but these materials themselves can stimulate innate immune responses. (jax.org)
Tiantan Puhua (pronounced Tee En Tan [rhymes with Ron] Pooh Hwah) Hospital is proud to be home to many of not only Asia's, but the world's leading doctors and researchers in neurosurgery, and conducts well over 4,000 brain operations each year. (mediescapes.com)
The creators of this repository are two researchers from CNIO, Manuel Valiente, head of the Brain Metastasis Group, and Eva Ortega-Paíno, director of the Biobank, who set out in the journal Trends in Cancer the advantages of the collection, called RENACER -Spanish acronym for the National Brain Metastasis Network-, which in just three years has compiled samples from more than 150 patients. (news-medical.net)
It's a strategy that helps to improve knowledge as well as diagnosis and treatment options, but also brings all the people involved closer together: patients, core researchers, chemical researchers, healthcare professionals, and the biobank. (news-medical.net)
Patients, '[because they act] as donors during a difficult brain metastasis neurosurgery, play a crucial role and we strongly believe that it is pivotal to empower them,' the researchers explain. (news-medical.net)
Researchers have produced an engineered tissue representing a simplified cerebral cortex by 3D printing human stem cells. (ox.ac.uk)
The researchers demonstrated for the first time that neural cells can be 3D printed to mimic the architecture of the cerebral cortex. (ox.ac.uk)
In this new study, the University of Oxford researchers fabricated a two-layered brain tissue by 3D printing human neural stem cells. (ox.ac.uk)
One way that researchers are attempting to improve the safety and efficacy of drugs is to use a relatively new area of research known as nanothrapeutics to target drug delivery just to the cells that need it. (cam.ac.uk)
Researchers can adjust the types and concentrations of the individual components, creating the optimal extracellular environment for their specific target cells. (labx.com)
At Lund Stem Cell Center, researchers are using innovative approaches to advance the field, offering exciting possibilities for the future. (lu.se)

5) it could be presented optimised with physiological tissue-derived cells, reversible as GC, IR, GC- MS, and HPLC, to account, infected, and provide measurements nearly and not, as forever to be the % of P and polypeptide site. (scoutconnection.com)

When a patient is injected with an anti-cancer drug, the idea is that the molecules will seek out and destroy rogue tumour cells. (cam.ac.uk)
A coating on the particles made them attracted to tumour cells from glioblastoma patients, so that the nanostructures bound and were absorbed into the cancer cells. (cam.ac.uk)
The process was so effective that 20 days later, the cell culture showed no evidence of any revival, suggesting that the tumour cells had been destroyed. (cam.ac.uk)
There is a marked survival advantage for patients with nonsmall cell lung cancer (NSCLC) expressing high numbers of macrophages in their tumour islets. (ersjournals.com)
Macrophages infiltrating the tumour islets in nonsmall cell lung cancer were predominantly of the M1 phenotype in patients with extended survival. (ersjournals.com)
Thus, increasing numbers of macrophages within the tumour islets conferred a marked survival advantage, while increased numbers of macrophages in the tumour stroma were associated with worse prognosis. (ersjournals.com)
The M1 phenotype (classically activated) macrophages are thought to be induced by interferon-γ, with or without lipopolysaccharide and tumour necrosis factor (TNF)-α, and exert a cytotoxic effect against cancer cells. (ersjournals.com)
Monocytes activated by tumour-derived microvesicles from pancreatic, colon and lung cancer cell lines have been found to show increased expression of human leukocyte antigen (HLA)-DR and a resulting increase in production of reactive oxygen intermediates and TNF-α 11 . (ersjournals.com)
Recent evidence, however, supports a dual role for macrophages in the regulation of tumour proliferation and immune control 10 , 16 , and indicates that the local tissue microenvironment plays a critical role in determining cell phenotypes. (ersjournals.com)

These cells typically do not maintain long-term glycemic control because they stimulate strong host-mediated immune reactions leading to cell necrosis and fibrosis. (jax.org)
Various preventive measures required to treat femoral head necrosis include revascularization, adequate supply of osteogenic cells and establishing enough strength to avoid collapse. (ijpsonline.com)
The main objective of this communication is to study the treatment of femoral head necrosis with cell replacement therapy and also its effects by focusing on the disease stages. (ijpsonline.com)

This will be achieved through the use of Bcl-2 protein-selective reagents, which were engineered in the Fairlie laboratory, to profile melanoma cell lines, as well as samples from patients. (edu.au)

Hematopoietic stem cell (derived from spleen, blood as well as bone marrow) and cancer cells can be developed in suspension. (microbiologynote.com)

Consistently fewer macrophages, neutrophils, β cells, and cytotoxic T cells were associated with TMTD alginate spheres compared to unmodified alginate spheres. (jax.org)
CD68 + macrophages expressing markers of a cytotoxic M1 phenotype or a noncytotoxic M2 phenotype were identified in the islets and stroma of surgically resected tumours from 20 patients with extended survival (median 92.7 months) and 20 with poor survival (median 7.7 months), using immunohistochemistry. (ersjournals.com)
The survival advantage conferred by islet macrophage infiltration may be related to their cytotoxic antitumour activity. (ersjournals.com)

Therefore, SiMeEx demonstrates advantages not only on time and resources, but also on the applicability in high-throughput studies. (frontiersin.org)
[ 2 ] Spodick also demonstrates that patients with improving congestive heart failure present with pseudoexduative effusion, likely secondary to rapid reabsorption of water compared with proteins and LDH in uncomplicated heart failure. (medscape.com)

METHODS: Prospective study including 989 consecutive adult cancer patients with high-risk of VTE. (unav.edu)

Complementary projects in the lab focus on autophagy, a cell survival process that has significant cross-talk with apoptosis. (edu.au)
There is some evidence that Bcl-2 pro-survival proteins (which normally keep cells alive by inhibiting the cell death program of apoptosis) are expressed at unusually high levels in melanoma compared to normal melanocytes. (edu.au)
Our hypothesis is that Bcl-2 pro-survival proteins provide a survival advantage for melanomas and might contribute to resistance to standard chemotherapeutic drugs. (edu.au)
Cells possess distinct pathways that promote their survival or death. (edu.au)
Communication between the cell survival pathway of autophagy and the cell death pathway of apoptosis is crucial for determining the best outcome for the cell, and ultimately its fate. (edu.au)
Results from this work will significantly advance our understanding of how the interplay between cell death and cell survival is regulated, which is still poorly understood despite its considerable biological importance. (edu.au)
This has extended the progression-free interval and prolonged survival in patients with various types of cancer. (biomedcentral.com)
The islet density of both M1 and M2 macrophages was markedly increased in extended compared with poor survival patients. (ersjournals.com)
Currently, even in patients with stage IA disease, the 5-yr survival after putatively curative surgical resection is only 67% 1 . (ersjournals.com)
The present authors have previously shown that the anatomical microlocalisation of macrophages in NSCLC strongly predicts patient survival, regardless of cancer stage 4 . (ersjournals.com)
The team believes that this approach holds the promise of enabling timely interventions, significantly enhancing treatment success rates, and ultimately improving patient survival rates. (lu.se)

The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. (wikipedia.org)
Even with close glucose monitoring, this strategy does not restore physiologic glycemic control, and patients typically suffer a wide range of secondary health complications. (jax.org)
They adhere to the vessel of culture through the help of an extracellular matrix , which typically comes from organ tissues which are immobile and encased within a network of connective tissues. (microbiologynote.com)
B-1 cells are found in peritoneal and pleural cavities where they provide first-line defence through antibodies able to bind polysaccharide antigens and repeated motifs that are typically found in microbial cell walls and macromolecules [ 2 , 3 ]. (biomedcentral.com)

Until recently, chlamydia prevention and patient care were impeded by the lack of suitable laboratory tests for screening and diagnosis. (cdc.gov)
There is need for adequate training of health care workers regarding diagnostics, intensive care of patients under isolation, contact tracing, adequate precautionary measures in handling infectious laboratory specimens, control of the vector as well as care and disposal of infectious waste. (folkhalsomyndigheten.se)
Included in the record were patient demographics, medical history (including specific comorbidities and condition of the wound), laboratory values, wound characteristics, digital imaging, and tracking of the wound over time. (medscape.com)
He showed an example of a 1-page OWEMR report that contained a list of medications, medical history, significant laboratory results, culture, radiology, vascular testing, pathology reports, and digital photos of a wound over time to illustrate the trend. (medscape.com)

For example, neural tissue development and central nervous system regeneration have been studied with organotypic explant culture. (wikipedia.org)
There, they are processed, organotypic cultures are created, and they are divided into proportional parts that are stored as samples for future investigations. (news-medical.net)

Over four weeks of culturing, the differentiated yet integrated tissues maintained their molecular, structural, and functional phenotypes, indicating their potential durability and reliability in immune-oncology applications. (corning.com)
In culture, the printed tissues maintained their layered cellular architecture for weeks, as indicated by the expression of layer-specific biomarkers. (ox.ac.uk)

Historically, explant culture has been used in several areas of biological research. (wikipedia.org)
Cellular grafts derived from retinal stem cell therapy is an active area of research to treat macular degeneration, retinitis, pigmentosa, and glaucoma. (wikipedia.org)
While all of the sessions are worth a watch, these four takeaways in particular leave a lasting impression about the future implications of 3D cell culture research. (corning.com)
In accordance with previous research , validation is particularly important given challenges with scalable production of these complex but powerful cultures. (corning.com)
Crown Bioscience is dedicated to increasing the number, variety, and quality of models available for drug development by leveraging its already established reputation in patient models by continuously investing in superior solutions for oncology research providing direction not only on the right candidate or combination to progress, but also the right patient. (businesswire.com)
These three areas combined to allow many more scientists to use cell, tissue and organ culture in their research. (microbiologynote.com)
At 18 Spanish hospitals, when a patient with brain metastasis undergoes surgery, they can donate a tiny part of their brain to the first repository of brain metastasis living samples in the world, based at CNIO (Spanish National Cancer Research Centre). (news-medical.net)
The hospitals involved with RENACER work as a network to pass on research findings to patients as quickly as possible. (news-medical.net)
According to the National Foundation for Cancer Research, more than half of newly diagnosed GBM patients die within the first 15 months. (sciencedaily.com)
To test the approach, the research team first used cultured cells to observe binding properties of the surfen compound. (sciencedaily.com)
Stand Up To Cancer works relentlessly to offer the newest, most effective, and most promising cancer treatments to patients quickly by bringing together the best minds to collaborate, innovate, and share cancer research. (standuptocancer.org)
Stand Up To Cancer was created to accelerate groundbreaking cancer research that will get promising new cancer treatments to patients quickly. (standuptocancer.org)
By isolating and reprogramming brain cells with dementia-causing genetic mutations, a team at JAX offers a powerful new research tool. (jax.org)
We want the scientific community to know about and take advantage of these new research resources. (jax.org)
Ph.D., professor and director of cellular engineering, has contracted with the National Institutes of Health to generate a collection of engineered iPSC brain cell lines for the Alzheimer's research community. (jax.org)
Ward was on a research team that achieved the first successful merger of stem cell-derived cell types and CRISPR screening technologies. (jax.org)
By epub Strategisches Controlling in international tätigen Konzernen: Aufgaben - Instrumente, joint cell grids make been creating the regulatory research Protein. (scoutconnection.com)
We hope that this new method will foster a culture of proactive health management and shift the paradigm towards preventive care, in addition to paving the way for more effective treatments," says Sandra Linstedt, research group leader at Lund Stem Cell Center. (lu.se)
The ICMR fosters a research culture by improving funding, mainly for developing infrastructure. (who.int)

The new advance builds on the team's decade-long track record in inventing and patenting 3D printing technologies for synthetic tissues and cultured cells. (ox.ac.uk)

Many neurobiological processes have been studied with retinal explant cultures. (wikipedia.org)

When it comes to the latest in stem cell healthcare, Tiantan Puhua Hospital doctors have developed a number of unprecedented and world leading stem cell treatment programs including 'Self stem cells Activation and Proliferation Program', neural stem cells implantation by Sterotacxios technique and Spinal Cord Stem Cell Injections. (mediescapes.com)

Although most of the melanomas that harbor BRAF mutations respond dramatically to treatment with BRAF inhibitors, nearly all develop resistance to the drugs in less than a year, and previous studies showed that melanomas alter a cell signaling pathway called the MAPK pathway to become resistant. (standuptocancer.org)
A promising new approach to finding effective treatments is to study human brain cells that carry mutations found in Alzheimer's patients. (jax.org)
He adds that by engineering disease-causing mutations in a set of genetically diverse iPS cells, "the project is designed to ensure reproducibility of data across laboratories and to explore the effect of natural variation in dementia. (jax.org)

They] can work by recruiting immune elements, delivering toxins, producing CAR-T cells, or as bispecific antibodies that recruit T or NK cells to kill the cancer cells. (labmanager.com)
This process is often deregulated in cancer cells, allowing them to survive and proliferate when otherwise they should be eliminated. (edu.au)
That could be clinically beneficial for a surgeon wanting to remove the tumor and not having to worry about rogue cancer cells. (sciencedaily.com)
In 2014, he and collaborators demonstrated that gold nanoparticles could be used to 'smuggle' chemotherapy drugs into cancer cells in glioblastoma multiforme, the most common and aggressive type of brain cancer in adults, which is notoriously difficult to treat. (cam.ac.uk)

The cells were then suspended in solution to generate two 'bioinks', which were then printed to produce a two-layered structure. (ox.ac.uk)

2000) Cartilage turnover in embryonic chick tibial explant cultures. (wikipedia.org)

These cells require to be fixed to solid or semi-solid substrate in order to increase their expansion. (microbiologynote.com)
After the cells have been isolated from the tissue and then proliferated in the right conditions, they take up all the substrate available i.e. reach confluence. (microbiologynote.com)
AMSBIO announce MatriMix-a groundbreaking 3D culture substrate. (labx.com)
The mycelium, a fibrous network derived from the substrate of a fungal culture, can take on the appearance of felt and has the advantage of being thermosettable, shock-resistance, breathable and water repellent. (premierevision.com)

Together, these data demonstrate that the TMTD-modified, larger microspheres induced significantly less innate immune cell stimulation and reduced fibrosis. (jax.org)

We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Once there is sufficient antibody titer in the serum, the spleen is removed to create hybridomas by fusing spleen cells with a cell line. (labmanager.com)
The American College of Obstetricians and Gynecologists (ACOG) has recommended that serum screening, cell-free DNA screening, and diagnostic tests, such as chorionic villus sampling (CVS) and amniocentesis , be discussed with and offered to all women early in pregnancy, regardless of their age. (medscape.com)
There were no significant differences upon presentation in age, sex, serum albumin, glycated hemoglobin , or white blood cell count between the patients who proceeded to amputation and those who did not. (medscape.com)

Up to now, however, there has been no method to ensure that implanted stem cells mimic the architecture of the brain. (ox.ac.uk)
The availability of animal models has established valuable and effective ocular level microenvironments that mimic human cells and help better understand disease patterns. (credihealth.com)

In an early study, they fused mouse myeloma and spleen cells from an immunized donor to produce cell lines that secreted anti-sheep red blood cell antibodies. (labmanager.com)

Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)

Genes that have been found to be associated with a certain disorder in human patients are knocked out or inserted into the genome of mice, rats or other animals. (eceae.org)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Anatomy7

Organisms10

Diseases7

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment112

Psychiatry and Psychology5

Phenomena and Processes62

Disciplines and Occupations15

Technology, Industry, Agriculture11

Information Science26

Named Groups1

Health Care39

Geographicals1

Fibroblasts6

Clinical19

Vitro14

Autologous3

Stromal2

Tissue culture7

Organoids16

Monolayer3

Patient's6

Progenitor cells3

20232

Regenerative Medicine2

IPSCs9

Adherent12

Mesenchymal1

Therapies7

Pluripotent5

Monolayers2

Growth17

Organoid5

Populations2

Viral8

Tumors3

Chemically-defined3

Infection11

Disadvantages2

Gene7

Transplantation3

Experiment2

Heterogeneous3

Biology3

Sensitivity5

Vivo6

Characterization1

Endothelial3

Isolates3

Symptomatic patients1

Blastocyst6

Researchers12

Physiological1

Tumour9

Necrosis3

Reagents1

Bone marrow1

Cytotoxic3

Demonstrates2

Adult1

Survival11

Typically4

Laboratory4

Organotypic2

Tissues maintained2

Research19

Synthetic1

Retinal1

Neural stem1

Mutations3

Cancer cells4

Generate1

Chick1

Substrate4

Significantly1

Hematopoietic1

Serum3

Mimic2

Donor1

Isolation1

Genes2