Passage stem cells. Medical search. Frequent questions

Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
In numerous clinical settings, mesenchymal stem cell treatment has already demonstrated significant therapeutic potential. (selfgrowth.com)
Moreover, it is also known that patients with chronic wound healing diseases have compromised mesenchymal stem cell functionality. (uni-muenchen.de)

Therefore, the authors investigated how the morphology, cell-surface markers, proliferation potential and differentiation capacity of tonsil-derived MSCs (T-MSCs) were affected by donor age, long-term passage, and cryopreservation. (pnuh.or.kr)
Authors evaluated the effects of donor-age, long-term passage, and cryopreservation on the morphology, surface markers, proliferation potential and differentiation capacities of T-MSCs. (pnuh.or.kr)
The effect of donor age, long-term passage and cryopreservation on the in vitro adipogenic, osteogenic, and chondrogenic differentiation potential of T-MSCs was not significant. (pnuh.or.kr)
The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
High variability and low consistency are major problems in manual stem cell culture processes, for both growth of stem cells and their differentiation into pre-defined cell types. (genengnews.com)
This minimizes fluctuations in environmental conditions that have been shown to impact on both cell growth over multiple passages and on the ultimate differentiation into specific cell types. (genengnews.com)
Advances in bioengineering have addressed problems of how to control growth and differentiation of stem cells using novel biomaterials, how to sort and purify specific stem cell products, and how to deliver and sustain stem cell grafts in vivo. (ca.gov)
Toward this goal, we propose to compare the behavior of nerve cell differentiation in multiple lines of hESCs in one laboratory setting. (ca.gov)
We will further characterize molecular changes during directed cell differentiation and identify the cells that exhibit a pattern of DNA modification, namely DNA methylation, similar to primary neural cells in human brain. (ca.gov)
We are now ready to compare the property of different lines of nerve cells such as the efficiency of nerve cell differentiation and the preferential production of specific nerve cells in culture. (ca.gov)
By engineering stem cells carrying different levels of methylation, we aim to find the optimal levels of DNA methylation for efficient nerve cell differentiation. (ca.gov)
Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

First CA Instit for Regenerative Med FDA Approved Trial Adult Stem Cells! (cbc-network.org)
The California Institute for Regenerative Medicine (CIRM) was sold to a gullible public on the promise of developing embryonic stem cell and therapeutic cloning CURES! (cbc-network.org)
For more than half a century, the regenerative possibilities of stem cells - which the body stores to repair damaged tissue and organs and restore blood supply - have tantalized the medical community. (truthdig.com)
The author or co-author of 363 published papers, Dr. Witte is the director of the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research. (huffpost.com)
The regenerative medicine bioprocessing program within the UCL department of biochemical engineering focuses on two areas: regenerative medicine translation and bioprocess engineering of stem cells. (genengnews.com)
A vocal activist for stem cell research, she speaks on behalf of the California Institute for Regenerative Medicine's (CIRM) investments in early stage research and clinical trials. (nih.gov)
In January, Shapiro was awarded a Stem Cell and Regenerative Medicine Action Award at the 2018 World Stem Cell Summit. (nih.gov)
Research carried out in the facility has made significant, high impact contributions in three areas of stem cell research: Molecular Mechanisms/Basic Science, Translational Bioengineering, and Regenerative Medicine. (ca.gov)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
In looking at regenerative therapies, stem cell therapy is one of the emerging options that appears to be quite promising. (betterhealthguy.com)

If you're studying a disease found in a certain part of the brain, you should use a human embryonic stem cell line that produces the neurons from that region of the brain to get the most accurate results from your study. (sciencedaily.com)
Sun said there are ways to prod an embryonic stem cell line biased to become neurons found in the rear brain to become neurons found in the forebrain. (sciencedaily.com)

OBJECTIVES: Human mesenchymal stem cells (MSCs) are efficacious in various cellular therapeutic applications and have been isolated from several tissues. (pnuh.or.kr)
T-MSCs isolated from donors of various ages were positive for markers CD90, CD44, and CD73, but negative for CD45, CD31, and HLA-DR. There were no significant differences in the expression of positive and negative surface markers as a function of donor age, long-term passage and cryopreservation. (pnuh.or.kr)
T-MSCs from different donor age groups showed similar proliferation potentials after passage 2. (pnuh.or.kr)
Cryopreservation did not affect the proliferation potential of T-MSCs, but there was a significant decrease in the proliferation potential in long-term passage T-MSCs (passage 15). (pnuh.or.kr)
CONCLUSION: The effect of donor age, long-term passage culture, and cryopreservation on T-MSC properties are negligible, except for the proliferation capacity of long-term cultured T-MSCs. (pnuh.or.kr)
Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
Mesenchymal stem cells (MSCs) in general may act by differentiating toward a particular cell type or by secreting various growth and trophic substances. (selfgrowth.com)
Mesenchymal stem cells (MSCs) may be a promising treatment option for ALS, as evidenced by clinical models. (selfgrowth.com)
The therapeutic effect of umbilical cord-derived mesenchymal stem cells(hUC-MSCs) in combination with pirfenidone(PFD) on pulmonary fibrosis in mice and its possible mechanism were investigated. (researchsquare.com)
MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)

Relapsing-remitting multiple sclerosis has been successfully treated using autologous hematopoietic stem cell therapy. (medindia.net)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
When abatacept is given intravenously to prevent graft versus host disease, it is usually injected slowly over 60 minutes on the day before hematopoietic stem-cell transplant and then on days 5, 14, and 28 after the transplant. (medlineplus.gov)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
60% of circulating tumor cells. (nature.com)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)

both neurotrophic factors only are present after passage 7 which should be taken into account for their utilization as a therapeutic option of neurodegenerative diseases. (sld.cu)
The purpose of this study is to examine the therapeutic efficacy of human umbilical cord mesenchymal stem/stromal cells (UMSCs) in treating congenital and acquired corneal opacity associated with the loss of collagen V. (molvis.org)
Summary of Research Progress: Our research aims to identify the optimal culture conditions and the best hESC lines for the derivation of nerve lineage cells in therapeutic cell transplantation. (ca.gov)

Notably, we found that Notch/RBPJ-bound regulatory elements are located adjacent to specific collagen genes in adult muscle stem cells, and investigated how extrinsic cues assure stem cell quiescence. (kcl.ac.uk)
An epic battle, 'Science, Politics, Stem Cells and Genes: CALIFORNIA'S WAR ON CHRONIC DISEASE' takes the reader behind the scenes. (mcnallyjackson.com)
Sun and her team confirmed that the two embryonic stem cell lines were different through gene expression analysis -- neurons that perform different functions in different parts of the brain express different genes. (sciencedaily.com)
Parents pass genes along to their offspring which equip them for their future life. (mpg.de)
However, even though all cells in the human body contain the same DNA, they "express" different genes to fulfill different functions. (mpg.de)
Epigenetics thereby represents an additional layer of information that helps cells to determine which genes to activate. (mpg.de)
But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
Their Stowers Institute for Medical Research is a not-for-profit organization in Kansas City that seeks to prevent and cure diseases by sponsoring research on genes that control the process of cells. (mdn.org)
Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
article{4b5db5d0-c1b6-463c-9ca2-f61b01429744, abstract = {{Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
Sickle cell disease is caused by genes your parents pass down to you. (msdmanuals.com)
You need two sickle cell genes, one from each parent, to have sickle cell disease. (msdmanuals.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

We also begin to produce and characterize a new type of human stem cells, namely induced pluripotent cells that are obtained by converting somatic cells into stem cell through reprogramming. (ca.gov)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

The new system also provides a valuable platform for fundamental studies of the culture microenvironment in stem cell biology. (genengnews.com)

When University of Minnesota officials announced recently they would pursue human embryonic stem cell research, they made a pledge to keep the controversial process open to public scrutiny. (publicradio.org)
The plan was presented by the Department of Health - Abu Dhabi for the project, which supports the provision of precision and personalised medicine through advanced treatment programmes that use human stem cells and tissues to develop medical research. (gulfnews.com)
Martin, a Catholic, expressed his opposition to stem cell research in 2007 by voting against legislation allowing the practice. (wikipedia.org)
A House Democrat has filed an amendment to the state budget that would bar the use of Department of Corrections funds for abortion, cloning or stem cell research. (missourinet.com)
The House has given first round approval to legislation meant to allow medical workers in Missouri refuse to participate in certain procedures including abortion, human cloning and stem cell research if they have a religious or moral objection to them. (missourinet.com)
A judge in Jefferson City has dismissed a lawsuit filed by a group known as the Missouri Roundtable for Life which is attempting to prevent taxpayer dollars from being spent on certain forms of stem cell research. (missourinet.com)
But, when that didn't work out - and desperate to show some progress for all the money CIRM has sucked up on Californians' credit card - it changed its tune and began funding research into adult stem cells - you know, the very ones that we had been told really didn't hold all that much promise? (cbc-network.org)
This is terrific news," says Ellen Feigal, M.D., Senior Vice President for Research and Development at California's stem cell agency, CIRM. (cbc-network.org)
I don't care if all it did was boost adult stem cell research, it should be defunded. (cbc-network.org)
Readers can expect a greater understanding of the intricate adventure of stem cell research, as well as the political wrestling it took to make progress possible - that California's effort may benefit the world. (mcnallyjackson.com)
Voted NO on expanding research to more embryonic stem cell lines. (ontheissues.org)
a technique that could potentially help to resolve the ongoing ethical debate over stem cell research. (progress.org.uk)
The bill, which would relax federal funding restrictions on human embryonic stem (ES) cell research, now awaits. (progress.org.uk)
Stem-cell research is being led by the Europeans. (thedailybeast.com)
Traditional methods for manual cultivation of stem cells often lead to high variability and low consistency, making development of robust experimental protocols for stem cell research extremely difficult. (genengnews.com)
Both academic and commercial collaborations are essential in this research, which addresses the complete stem cell process from donor or patient biopsy through to clinical implantation into the patient. (genengnews.com)
Recent advancements in stem cell research have given researchers new weapons in the fight against ALS and may also result in novel treatments for patients. (selfgrowth.com)
Preclinical research has shown that stem cells may be used to treat ALS, which is hopeful. (selfgrowth.com)
Small skin samples from people facing MND are all that are required for the creation of an endless supply of IPSCs in the laboratory, allowing for ongoing research on genetically similar cells to the patients. (selfgrowth.com)
Because she believes stem cell research will one day lead to a cure. (nih.gov)
The University of California at Santa Barbara (UCSB) is well positioned to make significant contributions by leveraging fundamental stem cell-based biomedical research efforts with enabling technologies in materials, microfluidics and bioengineering. (ca.gov)
The overall mission of this laboratory is to facilitate and promote stem cell research by investigators at UCSB, as well as those at neighboring universities, research institutes, and biotechnology companies on the California central coast. (ca.gov)
Past and current users of the Laboratory for Stem Cell Biology and Engineering include 52 researchers from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
Discoveries in fundamental basic research have revealed how human embryonic stem cells regulate gene expression to maintain pluripotency, how induced pluripotent stem cells retain a "memory" of their origin, and how adult stem cells differentiate in response to their environmental milieu. (ca.gov)
Past and current users of the Laboratory for Stem Cell Biology and Engineering include 58 researchers (6 new researchers in the past year) from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
They have new hope for treatment-and perhaps a cure-thanks to embryonic stem cell research. (abilitymagazine.com)
WASHINGTON (BP)-Senate advocates of stem cell research that destroys human embryos are trying a new tactic in their ongoing effort to gain federal funding for such experimentation. (baptistpress.com)
If the legislation were to become law, research on stem cells from embryos destroyed in private work between August 9, 2001, and June 15 of this year could receive federal grants for the first time. (baptistpress.com)
Bush rejected the Stem Cell Research Enhancement Act for the second time in less than a year. (baptistpress.com)
The bill would have provided funds for research using stem cells procured from embryos stored at in vitro fertilization clinics. (baptistpress.com)
Unlike research using embryos, extracting stem cells from non-embryonic sources such as umbilical cord blood, placentas, fat and bone marrow has nearly universal support. (baptistpress.com)
In December 1999, the editors of Science, the journal devoted to scientific and medical matters, called stem cell research the 'Breakthrough of the Year. (cbc.ca)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
Ontario and California together account for about 70 per cent of the stem cell research currently conducted in North America. (cbc.ca)
Some of that money would be aimed at turning the state into the second-largest stem cell research region in the United States. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
We have found a suitable substrate that can be used to enhance neuronal survival after cell transplantation and we expect to publish a research paper in this new method of cell transplantation. (ca.gov)
JEFFERSON CITY - A husband and wife, both cancer survivors, are the source of nearly 97 percent of the campaign funds in support of the stem cell research proposal on the November ballot, according to the Missouri Ethics Commission. (mdn.org)
Their institute, located in Kansas City, has warned that it will not go forward with its 600,000 square-foot expansion if Missouri voters reject the Stem Cell Research and Cures Initiative on Nov. 7 according to Connie Farrow, spokeswoman for the Coalition. (mdn.org)
James and Virginia believe that the new frontier is stem cell research," Farrow said. (mdn.org)
Washington (CNN) -- A federal court has given the Obama administration the go-ahead to continue funding embryonic stem-cell research. (cnn.com)
The U.S. Court of Appeals for the District of Columbia lifted an injunction imposed last year by a federal judge, who said all embryonic stem-cell research at the National Institutes of Health amounted to destruction of embryos, in violation of congressional spending laws. (cnn.com)
Legislation passed in 1996 law prohibits the use of taxpayer dollars in the creation or destruction of human embryos 'for research purposes. (cnn.com)
But the three-judge panel concluded in its 21-page ruling, 'the plaintiffs are unlikely to prevail because Dickey-Wicker is ambiguous and the NIH seems reasonably to have concluded' the law does not ban research using embryonic stem cells. (cnn.com)
The ruling does not deal with separate research on adult stem cells, which remains permissible under federal law. (cnn.com)
The issue at this stage deals only with the lifting of the injunction allowing funding to continue for embryonic stem-cell research. (cnn.com)
The field of embryonic stem-cell research has been highly controversial, because in most cases the research process involves destroying the embryo, typically four or five days old, after removing stem cells. (cnn.com)
Embryonic stem-cell research differs from other kinds of stem-cell research, which don't require embryos. (cnn.com)
When the injunction was first issued by Judge Royce Lamberth in August, Ron Stoddart, executive director of Nightlight Christian Adoptions -- one of the groups that filed the lawsuit -- said he supported adult stem-cell research that doesn't require destroying embryos. (cnn.com)
Frequently people will say, 'Why are you opposed to stem-cell research? (cnn.com)
When President George Bush first approved federal funding of human embryonic stem-cell research in 2001, 60 existing stem-cell lines -- which were created before August 9, 2001-- qualified for federal funding. (cnn.com)
Because such embryos are destroyed when stem cells are removed for research, most opponents believe this is moral issue. (cnn.com)
Supporters of embryonic stem-cell research say their studies have shown promise to treat a range of debilitating conditions including diabetes, Parkinson's disease, cancers, and spinal cord injuries. (cnn.com)
Under the supervision of Mattias Belting and co-supervision of Johan Bengzon and Charlotte Wellinder, h er research focused on understanding the mechanistic adaptation of glioblastoma cells, how they survive under microenvironmental stress and presented with potential novel fronts for targeted therapies. (lu.se)
At the end of the defense, the professors unanimously agreed that Myriam had passed with flying colors, and she is now officially a PhD graduate thanks to her hard work and dedication to the field of glioblastoma research. (lu.se)

The Senate Appropriations Committee has approved as part of a spending bill a measure that would grant government money for studies of stem cell lines derived from embryos over nearly the last six years. (baptistpress.com)
Extracting stem cells from embryos destroys the days-old human beings. (baptistpress.com)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
The case began with a lawsuit against the NIH by scientists opposed to use of embryonic stem cells, a group that seeks adoptive parents for human embryos created through in vitro fertilization, the nonprofit Christian Medical Association and others. (cnn.com)
We're opposed to the destruction of the embryos to get embryo stem cells. (cnn.com)

Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. (mdpi.com)
Effects and mechanism of hepatocyte growth factor-modified human adipose mesenchymal stem cells on wound healing of full-thickness skin defects in diabetic rats]. (bvsalud.org)
To investigate the effects and mechanism of hepatocyte growth factor (HGF)-modified human adipose mesenchymal stem cells (ADSCs) on the wound healing of full-thickness skin defects in diabetic rats . (bvsalud.org)
Results from a recent pilot study by Kuismanen et al suggested that urethral injection of a combination of patient-derived adipose stem cells (ASCs) and collagen can improve the symptoms of stress urinary incontinence in women, possibly providing a nonsurgical alternative to sling procedures for this condition. (medscape.com)

Stem cells are extremely sensitive to such changes in pH, and this leads to poor reproducibility in manual culturing, representing a major obstacle to both furthering our understanding of stem cell biology and the commercialization of stem cell applications. (genengnews.com)
We have developed and renovated a 1400 sq. ft. shared-use stem cell laboratory called the Laboratory for Stem Cell Biology and Engineering. (ca.gov)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
The second option that I considered was autologous stem cell therapy which is available in the United States. (betterhealthguy.com)

Information about the effects of donor age, long-term passage and cryopreservation are essential for clinical applications and cell-based therapies. (pnuh.or.kr)
There were no significant morphological differences according to donor age, long-term passage or cryopreservation. (pnuh.or.kr)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)

B) Analysis of stem cell proliferation marker gene LGR5 expression by quantitative reverse transcription PCR in undifferentiated HIEs. (cdc.gov)
B and T lymphocytes, CD4+ B lymphocytes versus CD8+ T lymphocytes), and tests that measure T-cell proliferation or function in response to specific or nonspecific stimuli (e.g., lymphocyte proliferation assays) ( 4,5 ). (cdc.gov)

Recent studies have reported that human tonsil tissue contains a new source of progenitor cells, potentially applicable for cell-based therapies. (pnuh.or.kr)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
Most solid tumors including lung cancer cells are believed to contain poorly differentiated cancer stem-like cells (CSCs) that initiate tumorigenesis and confer resistance to chemotherapy. (cdc.gov)
Similar to human non-small cell lung cancer H460 cells, SWCNT-transformed lung epithelial BEAS-2B cells but not its passage-matched control cells formed tumor spheres in stem cell-selective conditions, indicating the existence of lung CSCs. (cdc.gov)
Lung CSCs have previously been reported to reside in the side population (SP) of parental lung cancer cells. (cdc.gov)
Together, our results indicate the ability of SWCNT to transform human lung epithelial cells and induce CSCs which could represent a novel mechanism of carcinogenesis induced by CNTs. (cdc.gov)

In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
Greene said that amniotic stem cells derive their healing power from an ability to develop into any kind of tissue, but he failed to mention that mainstream science does not support his claims. (truthdig.com)
Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
Methods: Pulp tissue was collected from permanent third molars and digested and then the cells were seeded onto plates containing HDMEM medium. (bvsalud.org)
Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

Well, the first drug developed in part with direct CIRM grants has just received FDA approval for a clinical trial, and waddya know, they were derived from cardiac adult stem cells . (cbc-network.org)

Bone marrow transplants for cancer patients, which rely on blood stem cells, fulfill this potential. (truthdig.com)
Many clinics offer stem cells taken from a patient's own bone marrow or fat. (truthdig.com)
Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
For people who have weakened immune systems, especially people who have had an organ, stem cell or bone marrow transplant, CMV infection can be fatal. (mayoclinic.org)
People who have weakened immune systems, such as those who have had an organ, bone marrow or stem cell transplant, or those who are infected with HIV. (mayoclinic.org)
Aplastic crisis is when your bone marrow suddenly stops making new red blood cells and your anemia gets worse very quickly. (msdmanuals.com)

Despite Rep. Lundgren's intimations to the contrary, tumor growth is a problem bedeviling adult stem cell researchers, too . (motherjones.com)
To overcome this obstacle, researchers in the department of biochemical engineering at University College London (UCL) recently developed an automated stem cell culture system in a microplate format, offering higher consistency and reproducibility, better protection of cells from contamination, and improved operator safety. (genengnews.com)
Researchers previously had been able to produce neurons - the impulse-conducting cells in the brain and spinal cord - from human embryonic stem cells. (sciencedaily.com)
The researchers developed a more efficient and accessible process to apply CRISPR-Cas9 systems in human pluripotent stem cells (hPSCs), derived from federally approved stem cell lines, which Lian said could greatly advance diagnostics and treatments for genetic disorders. (eurekalert.org)
To improve the outcome, the researchers changed the way the genome editing tools are delivered to the stem cells, using modified RNA (modRNA). (eurekalert.org)
The researchers also found that the amount of time the modRNA was in place was ideal: long enough to modify the cells but not so long that it caused off-target activity. (eurekalert.org)
This is the process researchers want to better understand, so these are the cells they want to harvest and study. (eurekalert.org)
By focusing on the HA stem, the researchers believe the new vaccine candidate could provide wider and longer-lasting immunity. (medscape.com)
To create the vaccine candidate, researchers used the stem of an H1N1 influenza virus. (medscape.com)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)

Technically, manufacturers are required to submit stem cell therapies for review as a drug, and to provide evidence of their safety and efficacy, but the U.S. Food and Drug Administration hasn't enforced the rule consistently. (truthdig.com)
Numerous investigational therapies, many of which are currently in clinical trials like stem cell transplant for ALS , have been developed in response to recent advancements in our knowledge of the disease pathogenesis processes that underlie ALS. (selfgrowth.com)

On the other hand, the glial cell line-derived neurotrophic factor (GDNF) has the strongest trophic effect on dopaminergic neurons (10). (sld.cu)
Scientists were able to produce from human embryonic stem cells a highly pure, large quantity of functioning neurons that will allow them to create models of and study diseases such as Alzheimer's, Parkinson's, prefrontal dementia and schizophrenia. (sciencedaily.com)
However, the percentage of neurons in the cell culture was not high and the neurons were difficult to isolate from the other cells. (sciencedaily.com)
UCLA's Yi Sun, an associate professor of psychiatry and biobehavioral sciences, and Howard Hughes Medical Institute investigator Thomas Südhof at the University of Texas Southwestern Medical Center were able to produce 70 to 80 percent of neurons in cell culture. (sciencedaily.com)
We're excited because we have been able to purify so many more neurons out of the cell culture and they were, surprisingly, healthy enough to form synapses. (sciencedaily.com)
Sun's method prodded human embryonic stem cells to differentiate into neural stem cells, the cells that give rise to neurons. (sciencedaily.com)
When the time was right, Sun's team added protein growth factors into the cell culture that stopped the neural stem cells from self-renewing and prodded them into differentiating into neurons. (sciencedaily.com)
The neurons could then be separated from the neural stem cells that had not yet differentiated, a sort of chemical round-up that isolated the neurons. (sciencedaily.com)
The cells were then put into a cell strainer that allowed passage through of the isolated neurons. (sciencedaily.com)
Using the same techniques to prod the two embryonic stem cells lines to differentiate, Sun found that one line had a bias to become neurons that are found in the forebrain. (sciencedaily.com)
Huntington's disease, for example, is a forebrain disease, so the neurons should be differentiated from a cell line that is biased to produce neurons from the forebrain. (sciencedaily.com)
In order to ameliorate the unfavourable settings for sick motor neurons, stem cells take on a supporting function by establishing a fostering and neuroprotective milieu. (selfgrowth.com)
In this role, transplanted stem cells release neurotrophic factors, give rise to non-neuronal, non-sick cells like astrocytes and microglia, or develop into modulatory neurons that connect to damaged motor neurons. (selfgrowth.com)
Transplanted stem cells release neurotrophic factors and develop into accessory cells like astrocytes and microglia, creating a neuroprotective environment that can delay the degeneration of motor neurons. (selfgrowth.com)
Fortunately, ALS-affected neurons can be accurately mimicked by a type of stem cell termed an Induced Pluripotent Stem Cell (IPSC). (selfgrowth.com)
Because of this, stem cell transplants are currently employed to preserve a patient's healthy neurons while also offering the possibility of producing new cells to replace the deceased ones. (selfgrowth.com)
The resulting cells are nonproliferating and present an alternative to induced pluripotent stem cells for obtaining patient- and disease-specific neurons to be used for disease modeling and for development of cell therapy. (lu.se)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)

This shouldn't be surprising-with either adult or embryonic stem cells, scientists are dealing with, and manipulating, cell growth and death and division. (motherjones.com)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
Some scientists believe embryonic stem cells could help treat many diseases and disabilities because of their potential to develop into many different cell types in the body. (cnn.com)

The mechanisms by which niche molecules and intrinsic factors regulate muscle stem cell quiescence and properties remain largely unknown. (kcl.ac.uk)
By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)

No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)

In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
C) Heat map based on log (2 -∆∆Ct ) comparing gene expression levels for markers of differentiated small intestinal epithelial cells between undifferentiated and 4-day differentiated HIE monolayers. (cdc.gov)
Shown are markers for enterocytes (EC), Paneth cells (PC), enteroendocrine cells (EE), goblet cells (GC), and stem cells (SC). (cdc.gov)
The long spindle-shaped primary ADSCs were obtained by collagenase digestion , and the third passage of cells were identified by flow cytometry to positively express ADSCs surface markers CD29 and CD90 and negatively express CD34 and CD45. (bvsalud.org)

Is Stem Cell Therapy Safe for Relapsing-Remitting Multiple Sclerosis Treatment? (medindia.net)
Medications weren't working and she had lost hope until she found adult stem cell therapy. (ksat.com)
Some opponents have called into question the safety of stem cell therapy because it's so new. (ksat.com)
Once Cowley got involved in the fight to bring stem cell therapy to the United States, she began meeting many other patients who also travel for their adult stem cell treatment. (ksat.com)
In January, District 63 state Rep. Tan Parker introduced House Bill 810 to allow stem cell therapy in Texas. (ksat.com)
The development of the disease condition might be slowed down by stem cell therapy. (selfgrowth.com)
This makes stem cell therapy an appealing treatment option for ALS since it takes on the disease's intricate evolution through a number of processes. (selfgrowth.com)
Supportive care and empiric antibiotic therapy are the mainstays of successful passage through this phase. (medscape.com)
The first option was embryonic stem cell therapy in India. (betterhealthguy.com)
The third option that I was aware of was umbilical stem cell therapy in Panama. (betterhealthguy.com)
So, after months of researching and weighing the options, I decided to proceed with umbilical stem cell therapy in Panama. (betterhealthguy.com)
In late August 2010, I went to Panama to have stem cell therapy. (betterhealthguy.com)
I also felt like I didn't really have anything to say early on in the process and honestly, I'm still forming my opinion on the benefits of the stem cell therapy. (betterhealthguy.com)
You actually need a combination therapy … to actually stop cells from dying and that's what hypothermia does. (spinalcordinjuryzone.com)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
One such idea is cell replacement therapy (CRT). (lu.se)

Abu Dhabi: Plans for a Biobank of stem cells and tissues, for use in advanced treatments, have been approved in Abu Dhabi. (gulfnews.com)
Stem cells are the body's master cells that can develop into tissues and other cells, providing hope for the treatment of numerous afflictions. (baptistpress.com)
He shared with me that embryonic cells are pluripotent and can become muscle, heart, nerve, and other tissues but that they have a risk of uncontrolled tumor growth. (betterhealthguy.com)

A tiny vial no larger than the palm of his hand, he told the group, contains roughly 10 million live stem cells, harvested from the placenta, amniotic fluid, umbilical cord or amnion, the membrane that surrounds the fetus in the womb. (truthdig.com)

As predicted, the US House of Representatives has approved - with a 247-176 majority - the version of the Stem Cell Enhancement Act 2007 that the Senate passed earlier this month. (progress.org.uk)

The bioprocess engineering aspect of this work deals with the critical challenge of developing reliable processes for the preparation of homogenous populations of stem cells and their progeny. (genengnews.com)
The ones that fix themselves can pass the repair, or "mutation," to their progeny. (eurekalert.org)

Stem cell transplant for ALS: Can the curse be changed to a boon? (selfgrowth.com)

The Food and Drug Administration said cells removed from someone's body for more than 24 hours become a drug and are subject to testing and clinical trials. (ksat.com)
In clinical trials, stem cells are being utilised to treat ALS effectively. (selfgrowth.com)
She hopes families' participation in stem cell clinical trials will improve the odds for many more patients when such a cure becomes available. (nih.gov)
The first clinical trials involving a patient receiving human embryonic stem cells began in October 2010 at the Shepard Center, a spinal cord injury hospital in Atlanta. (cbc.ca)

L ike the three previous generations of mothers in her family, Adrienne Shapiro gave birth to a child born with sickle cell disease. (nih.gov)
Yet the news came as a complete surprise-not because she was unaware of her family history, but because she had received a false negative result from her sickle cell disease/trait test. (nih.gov)
Shapiro arrived on the pediatric floor to find the attending doctor at his desk looking up sickle cell disease in his medical book. (nih.gov)
Watching how she took care of my brother [who has sickle cell disease], and her belief that science would one day cure sickle cell disease, gave me the motivation I have today. (nih.gov)
Sickle cell disease is a lifelong problem with your red blood cells. (msdmanuals.com)
If the other copy of the gene is normal, then you don't have sickle cell disease. (msdmanuals.com)
If two people with sickle cell trait have children, half of their children will have the trait, one quarter will have sickle cell disease, and one quarter will be normal. (msdmanuals.com)
People with sickle cell disease (not just the trait) are often unable to have children. (msdmanuals.com)
What are the complications of sickle cell disease? (msdmanuals.com)
Not all red blood cells have the sickle shape when you have sickle cell disease. (msdmanuals.com)
What are the symptoms of sickle cell disease? (msdmanuals.com)

These cells will be excellent for doing gene expression studies and biochemical and protein analyses. (sciencedaily.com)

Regulation of skeletal muscle stem cells during homeostasis and regeneration involves the interplay of extrinsic and intrinsic cues. (kcl.ac.uk)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

In the past year, we have made progress in guiding several lines of human stem cells into nerve cells. (ca.gov)
We also test the pattern of DNA methylation in different lines of human stem cells. (ca.gov)

Our previous studies have shown that chronic exposure to single-walled carbon nanotube s (SWCNTs) induces malignant transformation of human lung epithelial cells. (cdc.gov)
In this study we investigated whether SWCNT-transformed lung epithelial cells present CSC traits. (cdc.gov)

MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
The cell morphology was observed under an inverted phase contrast microscope, and the transfection rate was calculated. (bvsalud.org)

Alongside scientifically proven treatments, an industry has sprung up in which clinics offer miracle remedies from stem cell products. (truthdig.com)

Finally, cell transplantation in a mouse stroke model will be used to study the mechanisms and efficacy of different types of hESC-derived neural cells in neural repair. (ca.gov)
In addition, because the cells do not pass a stem cell intermediate, direct neural conversion has the potential to be performed in vivo. (lu.se)
Taken together, our data provide proof of principle that direct neural conversion can take place in the adult rodent brain when using transplanted human cells or endogenous mouse cells as a starting cell for neural conversion. (lu.se)

He also announced at a White House ceremony he had signed an executive order to encourage the government's attempts to underwrite ethical stem cell studies. (baptistpress.com)

2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

These include spinal cord injuries, rheumatoid arthritis, lupus, multiple sclerosis and sickle cell anemia. (baptistpress.com)
The central nervous system is composed of the brain, brain stem, and the spinal cord. (medscape.com)

All users working with Gene Modified Models (GMM) must be aware that in order to work in our facility it is necessary that you include Lund Stem Cell Center FACS core facility (Room B1241a and B1213a) in your F-permit. (lu.se)

Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)

The aim of this study is to learn if rat BMSC express brain-derived neurotrophic factor (BDNF) and glia-derived nerve factor (GDNF) and determine if this expression varies according to the number of culture passages. (sld.cu)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Anatomy61

Organisms16

Diseases11

Chemicals and Drugs60

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Phenomena and Processes52

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care1

Mesenchymal stem3

Differentiation19

Regenerative10

Embryonic Stem Cell2

MSCs13

Hematopoietic4

Tumor cells4

Therapeutic3

Genes13

Somatic2

Microenvironment1

Research56

Embryos7

Adipose4

Biology3

Autologous2

Donor4

Proliferation2

Progenitor cells2

CSCs7

Tissue10

CIRM1

Bone6

Researchers10

Therapies2

Neurons19

Enrichment1

Scientists5

Regulate2

Pluripotent cell1

Markers5

Therapy16

Tissues3

Umbilical cord1

Enhancement Act1

Progeny2

Transplant1

Clinical trials4

Sickle cell di11

Gene expression1

Regulation2

Human stem cells2

Epithelial cells2

Morphology2

Treatments1

Neural3

Ethical1

Intrinsic1

Spinal cord2

FACS1

Diseases1

Expression2