• viral
  • 1 , 2 This muscle-related condition is also known as influenza-associated myositis, viral myositis , and acute myositis . (cfp.ca)
  • Viral replication is initiated only after successful entry into a cell by attachment and fusion between the virus and the host cell lipid membrane. (wikipedia.org)
  • Subsequently, genomic transcription occurs using the viruses own 'viral RNA-dependant RNA polymerase' (L protein). (wikipedia.org)
  • Because the storage conditions might have damaged virus capsids (and thus made the viral nucleic acid vulnerable to nuclease digestion) we modified the previously described purification procedure 1 by eliminating the use of nucleases. (pubmedcentralcanada.ca)
  • This method is based on physical separation of host and virus nucleic acids followed by a degenerate oligonucleotide primer PCR (DOP-PCR) that is optimized for virus-sized genomes and permits non-specific nucleic acid amplification at a high degree of sensitivity (100-1000 copies of viral nucleic acid). (pubmedcentralcanada.ca)
  • Influenza and parainfluenza viral cultures were also performed. (springer.com)
  • Among the seven viruses/genotypes detected with higher frequencies, multiplex PCR sensitivities were correlated significantly with viral loads determined by the TaqMan RT-PCR in FluA, H1N1-p and RSV ( p =0.011−0.000). (springer.com)
  • The virucidal activity of CV-N against influenza virus is directed towards viral haemagglutinin. (wikipedia.org)
  • In recent years, remarkable progress has been made in determining, at atomic and subnanometeric levels, the structures of a number of key viral proteins and of the virion capsids of several dsRNA viruses, highlighting the significant parallels in the structure and replicative processes of many of these viruses. (wikipedia.org)
  • RNA viruses generally have very high mutation rates compared to DNA viruses, because viral RNA polymerases lack the proofreading ability of DNA polymerases. (wikipedia.org)
  • Some genes of RNA virus are important to the viral replication cycles and mutations are not tolerated. (wikipedia.org)
  • There are three distinct groups of RNA viruses depending on their genome and mode of replication: Double-stranded RNA viruses (Group III) contain from one to a dozen different RNA molecules, each coding for one or more viral proteins. (wikipedia.org)
  • Positive-sense ssRNA viruses (Group IV) have their genome directly utilized as mRNA, with host ribosomes translating it into a single protein that is modified by host and viral proteins to form the various proteins needed for replication. (wikipedia.org)
  • Reverse transcriptase, a viral enzyme that comes from the virus itself after it is uncoated, converts the viral RNA into a complementary strand of DNA, which is copied to produce a double-stranded molecule of viral DNA. (wikipedia.org)
  • airway
  • There are few studies that have used the World Health Organization's (WHO) [ 3 ] clinical diagnostic criteria together with current extended molecular methods to examine the relationship between both upper airway viruses and bacteria and clinical and/or radiologically confirmed pneumonia, and its outcomes, in children. (springer.com)
  • The majority of studies have focused on specific viruses [ 4 , 5 ] or bacteria [ 6 , 7 , 8 ], with few assessing whether upper airway microbiology predicts clinical symptoms. (springer.com)
  • croup
  • Population-based data indicate that 1%-5% of children with croup are admitted to hospital, 8 - 10 and, of those admitted, less than 3% receive intubation. (cmaj.ca)
  • pathogen
  • Nipah virus was first identified as the pathogen responsible for outbreaks of encephalitis in Malaysia and Singapore from October 1998 to June 1999 ( 1 - 6 ). (cdc.gov)
  • A case of pulmonary infiltrates and fever of unknown origin occurring in a hematopoietic stem cell transplant (HSCT) recipient was investigated using a modified version of the generic virus detection assay after conventional methods failed to identify a pathogen. (pubmedcentralcanada.ca)
  • The pathogen-containing particles, also called Flügge droplets (after Carl Flügge), are 0,1-2 mm in diameter, and are reduced by evaporation to droplet nuclei - small (smaller than 100 μ in diameter), dry particles that can remain airborne for long periods. (wikipedia.org)
  • Bluetongue virus is an economically important pathogen of cattle and sheep. (wikipedia.org)
  • Pneumonia
  • In Indigenous children hospitalised with pneumonia in Central Australia, we describe the nasopharyngeal detection of viruses and bacteria and assessed whether their presence related to signs of pneumonia (tachypnoea and/or chest in-drawing) on hospital admission and during subsequent days. (springer.com)
  • To identify the potential cause of the pneumonia using a degenerate oligonucleotide primer (DOP) PCR assay which is designed to detect all viruses. (pubmedcentralcanada.ca)
  • hepatitis delta virus
  • 5. Use according to any preceding claim where the nucleic acid molecule is a modified hepatitis delta virus genome. (google.it)
  • 6. Use according to any preceding claim where the nucleic acid molecule is a modified hepatitis delta virus genome comprising 1.2 copies of the genome with an ORF encoding the delta antigen inactivated by a 2 nucleotide deletion. (google.it)
  • protein
  • Heterologous Expression and Isolation of Influenza A Virus Nuclear Export Protein NEP. (amedeo.com)
  • Cullin-4A is a protein that in humans is encoded by the CUL4A gene. (wikipedia.org)
  • For example, the region of the hepatitis C virus genome that encodes the core protein is highly conserved, because it contains an RNA structure involved in an internal ribosome entry site. (wikipedia.org)
  • pathogens
  • Over the last decade, PCR has promised the rapid, sensitive and accurate detection of many pathogens, e.g. viruses, even when present in low numbers. (google.it)
  • Proteins
  • The pathogenicity of HPIVs is mutually dependent on the viruses having the correct accessory proteins which are able to elicit anti-interferon properties. (wikipedia.org)
  • Matrix proteins inside the envelope stabilise virus structure. (wikipedia.org)
  • distinct
  • The taxonomic division is broadly based on antigenic and genetic characteristics, forming four major serotypes or clades, which today are considered distinct viruses. (wikipedia.org)
  • nucleotides
  • A 5' trailer sequence, 50-161 nucleotides long Intergenomic regions between each gene, which are three nucleotides long for morbilliviruses, respiroviruses and henipaviruses, and variable length (1-56 nucleotides) for rubulaviruses. (wikipedia.org)
  • nasopharyngeal
  • In these cases, French investigators utilized improved techniques for recovering the virus from nasopharyngeal aspirates and from stool samples. (wikipedia.org)
  • genome
  • Negative-sense ssRNA viruses (Group V) must have their genome copied by an RNA replicase to form positive-sense RNA. (wikipedia.org)
  • Retroviruses (Group VI) have a single-stranded RNA genome but, in general, are not considered RNA viruses because they use DNA intermediates to replicate. (wikipedia.org)
  • nucleic acid
  • 1. Use of a nucleic acid molecule having intra-molecular base pairing as an internal control in nucleic acid amplification. (google.it)
  • 2. Use according to claim 1 where the nucleic acid molecule is RNA. (google.it)
  • We previously developed and optimized an improved universal virus detection method that sensitively and broadly detects DNA and RNA viruses in infected cell cultures and spiked cells with limiting amounts of nucleic acid or viruses 1 . (pubmedcentralcanada.ca)
  • Jain
  • Data from Tippett and Clark, 1 Jain and Kolber, 2 and Mackay et al. (cfp.ca)
  • It was first isolated in 1983 by Rafael Apitz-Castro and Mahendra K. Jain (patent US4665088 [1]) Its basic organic chemistry has been extensively investigated. (wikipedia.org)
  • affects
  • 1 , 2 It affects boys more than girls (1.4:1) and young children between 6 months and 3 years of age more commonly than younger infants, older children and adolescents. (cmaj.ca)
  • classification
  • The International Committee on Taxonomy of Viruses (ICTV) classifies RNA viruses as those that belong to Group III, Group IV or Group V of the Baltimore classification system of classifying viruses and does not consider viruses with DNA intermediates in their life cycle as RNA viruses. (wikipedia.org)
  • onset
  • Although far less than 1% of children with acute-onset stridor have another diagnosis, clinicians should consider an alternate cause if children do not respond to standard therapy or appear extremely unwell ( Box 2 ). (cmaj.ca)