Introduction: This review is an update on recent progress in proteomic studies of formalin-fixed paraffin-embedded (FFPE) tissues, which open the opportunity to investigate diseases and research potential biomarkers, particularly when availability of fresh/frozen tissues is low. (medscape.com)
In this study, we attempted to detect these fusion genes using formalin-fixed paraffin-embedded (FFPE) tumor tissues and investigated their clinical utility and factors that affect the results of examination. (go.jp)
Detection of fungal DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is challenging due to degradation of DNA and presence of PCR inhibitors in these samples. (biomedcentral.com)
One obstacle to clinical translation is that these signatures were developed using frozen tissue, whereas usually only formalin-fixed, paraffin embedded (FFPE) tissue is available. (nih.gov)
However, routine application of smFISH to formalin-fixed, paraffin-embedded (FFPE) samples is challenging due to the low signal intensity and high background noise. (unito.it)
Use of archival resources has been limited to date by inconsistent methods for genomic profiling of degraded RNA from formalin-fixed paraffin-embedded (FFPE) samples. (nih.gov)
The purpose of this paper was to compare the yield and purity of RNA extracted using three different extraction methods from sections of a formalin-fixed paraffin-embedded (FFPE) colon biopsy, and to compare real-time RT-PCR cycle threshold (CT) values among two extraction methods, three reverse transcription priming methods, and specimens where preamplification was performed and those where it was not. (nih.gov)
Therefore, we have employed a systematic approach to develop, establish and validate an optimized "standard operating protocol" (SOP) for sample preparation in MALDI MSI of formalin-fixed paraffin-embedded (FFPE) tissue sample analyses within this study. (maastrichtuniversity.nl)
Formalin fixation and paraffin embedding (FFPE) is most often used for sample preservation though fresh frozen tissue or disaggregated cells are sometimes used for specific downstream applications. (biomedcentral.com)
From traditional tissue biopsy the most common pathological preservation path is through formalin fixation and paraffin embedding (FFPE), though fresh frozen tissue or disaggregated cells are sometimes also available. (biomedcentral.com)
The majority of the samples are formalin-fixed paraffin-embedded (FFPE) tissues. (cancer.gov)
Tissues6
Update on proteomic studies of formalin-fixed paraffin-embedded tissues. (medscape.com)
Validation of ovarian cancer gene expression signatures for survival and subtype in formalin fixed paraffin embedded tissues. (nih.gov)
Fixed tissues are then processed for paraffin embedding. (fdneurotech.com)
or (3) fresh frozen blocks in OCT medium, stored at -80°C. Fixed tissues are distributed as unstained sections on glass slides or paraffin scrolls. (nih.gov)
Specificity of the primary antibodies in formalin-fixed, paraffin-embedded tissues is not demonstrated and cannot be assumed. (cdc.gov)
Fixed paraffin embedded tissue4
Formalin fixed paraffin embedded tissue occasionally requires reprocessing if the histologic quality of a section is inadequate for clinical diagnosis. (nih.gov)
Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors? (biomedcentral.com)
The described nested RT-PCR method as another supportive diagnostic method enables pathologists to differentiate small blue cell tumors not only to make correct diagnosis but also to investigate retrospective archival tumor samples, using formalin fixed paraffin embedded tissue as a source of RNA. (korea.ac.kr)
Include unstained slides and/or a formalin-fixed, paraffin-embedded tissue block if it is anticipated that additional stains or ancillary testing may be necessary. (uwhealth.org)
Formalin11
One biopsy was collected during a colonoscopy and fixed in 10% neutral buffered formalin for 24 h before being embedded in paraffin and stored at room temperature. (nih.gov)
Recent developments in spatial proteomics have paved the way for retrospective in situ mass spectrometry (MS) analyses of formalin-fixed paraffin-embedded clinical tissue samples. (edu.au)
Recently, formalin-fixed paraffin-embedded MALDI imaging analyses were augmented to allow in situ analyses of tissue-specific N -glycosylation profiles. (edu.au)
Detection and quantification of hippocampal synaptophysin messenger RNA in schizophrenia using autoclaved, formalin-fixed, paraffin wax-embedded sections. (ox.ac.uk)
To do this, we targeted synaptophysin messenger RNA with a 35S-labelled oligonucleotide probe in autoclaved, formalin-fixed, paraffin wax-embedded sections of the hippocampal formation of 11 schizophrenics and 11 controls. (ox.ac.uk)
We conclude that autoclaving renders formalin-fixed, paraffin wax-embedded sections of human brain suitable for quantitative in situ hybridization histochemistry. (ox.ac.uk)
The suitability of formalin-fixed and paraffin-embedded tumor material was evaluated for molecular analysis of the RET proto-oncogene. (uab.cat)
Whole Genome Sequence of a Treponema pallidum Strain From a Formalin-Fixed, Paraffin-Embedded Fine Needle Aspirate of a Cervical Lymph Node. (bvsalud.org)
Formalin-fixed tissue - The contralateral hemisphere is fixed in 15% buffered formalin, embedded in agar, and sliced coronally at 3 mm intervals. (nih.gov)
Formalin fixation and paraffin embedding, which has long been the default pathological biopsy medium, is now being supplemented with liquid biopsy as a means to profile the cancer genomes of patients. (biomedcentral.com)
The authors report immunohistochemical (IHC) results on formalin-fixed, paraffin-embedded, autopsy brain tissue from these two cases. (cdc.gov)
Fixation3
Before diagnosis, patient samples are typically processed by fixation and paraffin embedding. (nih.gov)
This fixation/embedding process is used to preserve tissue morphology and histology for subsequent evaluation. (nih.gov)
Tartrate-resistant acid phosphatase activity may be demonstrated in paraffin-embedded liver and spleen specimens as well as in decalcified bone marrow biopsies after fixation in a mixed glutaraldehyde-formaldehyde-calcium acetate solution. (bmj.com)
Specimens1
8. Fluorescent in situ hybridization on isolated tumor cell nuclei: a sensitive method for 1p and 19q deletion analysis in paraffin-embedded oligodendroglial tumor specimens. (nih.gov)
PAXgene Tissue1
PAXgene Fixed, Paraffin Embedded (PFPE) Tissue - Aliquots, similar in size and adjacent to those described above, that are fixed in PAXgene Tissue fixative and paraffin embedded. (nih.gov)
Slides2
All paraffin embedded embroyos are cut sagittally at 7 µm and mounted on Superfrost ® Plus or Fisherbrand ProbeOn™ microscope slides (2 consecutive sections per slide). (fdneurotech.com)
All pending and final outside pathology reports must be included with the slides/paraffin blocks. (uwhealth.org)
Histotechnicians performed a subjective evaluation of all the samples at the time of embedding and microtomy. (nih.gov)
Conclusion: Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β-globin gene amplified in PCR, although the results suggest that this is a way to increase the method for efficacy amplification of PCR. (bvsalud.org)
Sections3
As a result, the efficiency and the number of intact paraffin sections were greatly improved. (jove.com)
Diagnosis of hairy-cell leukaemia by tartrate-resistant acid phosphatase activity in paraffin-embedded tissue sections. (bmj.com)
Introduction: Several reasons may lead to the failure of polymerase chain reaction (PCR) using DNA purified from paraffin-embedded materials: presence of inhibitors and degradation of target DNA. (bvsalud.org)
Block2
Use a knife to modify the paraffin block acquired by paraffin embedding. (jove.com)
This is a video that demonstrates the general method for embedding tissue in a paraffin block. (protocolsonline.com)
Morphology1
Spheroids embedded in fibrin matrix are easily processed using showing morphology of spheroid. (nih.gov)
Detect1
polymerase chain reaction (PCR), it was possible to detect point mutations at codon 12 of the K-ras oncogene from 25-year-old paraffin-embedded normal lungs and lung adenocarcinomas from mice exposed to radiation. (nih.gov)
Material1
Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated at the Oral Oncology Center were selected. (bvsalud.org)
Standard1
A better tissue culture model of histologically processed using standard paraffin embedding techniques. (nih.gov)