• In enzymology, a formate dehydrogenase (cytochrome-c-553) (EC 1.2.2.3) is an enzyme that catalyzes the chemical reaction formate + ferricytochrome c-553 ⇌ {\displaystyle \rightleftharpoons } CO2 + ferrocytochrome c-553 Thus, the two substrates of this enzyme are formate and ferricytochrome c-553, whereas its two products are CO2 and ferrocytochrome c-553. (wikipedia.org)
  • Prosthetic group of: formate dehydrogenase, purine hydroxylase, thiosulfate reductase. (wikipedia.org)
  • Schauer, N.L. and Ferry, J.G. FAD requirement for the reduction of coenzyme F 420 by formate dehydrogenase from Methanobacterium formicicum . (enzyme-database.org)
  • In this work, the co-immobilization of formate dehydrogenase (FDH) and glycerol dehydrogenase (GlyDH) enzymes is proposed to reduce CO 2 into formic acid, an important chemical intermediate. (pucv.cl)
  • Furthermore, a great increase in the retained activity for the formate dehydrogenase enzyme was noted, passing from 18% to 89%, when the mesostructured clinoptilolite was used as support. (pucv.cl)
  • The immobilization yield of formate dehydrogenase and glycerol dehydrogenase is around 100% with all the supports studied. (pucv.cl)
  • Genes and proteins that showed similar trends in detected levels were particularly involved in energy conservation such as increases in an annotated ech hydrogenase, formate dehydrogenase, pyruvate:ferredoxin oxidoreductase, and rnf oxidoreductase, and the biofilm cells had elevated formate dehydrogenase activity. (escholarship.org)
  • protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
  • This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. (arel.edu.tr)
  • The in situ enzymatic digestion of the two proteins separated in 2D-SDS-PAGE gels, followed by microsequencing of the peptides, resulted in the identification of these two proteins as related to human lipoamide dehydrogenase and thiol: protein disulfide oxidoreductase (TPDO). (psu.edu)
  • In common, both these proteins have a bis (cysteinyl) sequence motif cys-X-X-cys (for lipoamide dehydrogenase it is cys-X-X-X-X-cys) which is also an integral part of several other proteins such as thioredoxin, protein disulfide isomerase, endoplasmic reticulum protein (ERp72), selenoprotein W, 56 kDa acetaminophen binding protein and formate dehydrogenase. (psu.edu)
  • Tailoring of recombinant FDH: effect of histidinetag location on solubility and catalytic properties of Chaetomium thermophilum formate dehydrogenase (CtFDH). (moleculardepot.com)
  • The pulse of acetogenesis appears to have been collectively catalyzed by a number of different organisms and metabolisms, most prominently pyruvate:ferredoxin oxidoreductase. (frontiersin.org)
  • When the pfl1 mutants were subjected to dark fermentative conditions, they displayed an increased flux of pyruvate to lactate, elevated pyruvate decarboxylation, ethanol accumulation, diminished pyruvate oxidation by pyruvate ferredoxin oxidoreductase, and lowered H2 production. (montana.edu)
  • Glycine radical, Pyruvate formate lyase-like [Interproscan]. (ntu.edu.sg)
  • A preliminary test for the enzymatic conversion of D-xylose into xylitol by the intact cells of C. pelliculosa (xylose reductase) coupled with the intact cells of a formate-utilizing methanogen strain HU (hydrogenase and F420-NADP oxidoreductase) was conducted by using H2 as an electron donor of NADP+. (eurekamag.com)
  • B1t R2â2ç2 MCD Comamonas testosteroni 63u R2â2ç2 MCD isoquinoline 1-oxidoreductase Pseudomonas diminutav Râ MCD quinoline-4-carboxylate-2-oxidoreductase Agrobacterium sp. (slideserve.com)
  • In this multi-stage process, fermentative bacteria hydrolyze the polymeric substrates present in all anaerobic habitats and then ferment the hydrolysis products to acetate and longer chain fatty acids, alcohols, aromatic compounds, CO 2 , formate, and H 2 . (ucla.edu)
  • Lastly, two different groups, the hydrogen/formate-using and acetate-using methanogens, complete the process by converting the acetate, formate and hydrogen to CO 2 and CH 4 . (ucla.edu)
  • The consumption of the acetate, hydrogen and formate by the methanogen partner keeps the intermediates at the very low concentrations necessary for the overall degradative process to be energetically favorable. (ucla.edu)
  • Caloramator celer is a strict anaerobic, alkalitolerant, thermophilic bacterium capable of converting glucose to hydrogen (H 2 ), carbon dioxide, acetate, ethanol and formate by a mixed acid fermentation. (biomedcentral.com)
  • Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate. (mpg.de)
  • This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with a cytochrome as acceptor. (wikipedia.org)
  • The systematic name of this enzyme class is formate:ferricytochrome-c-553 oxidoreductase. (wikipedia.org)
  • The enzyme, characterized from methanogenic archaea, is involved in formate-dependent H 2 production. (enzyme-database.org)
  • To further probe the system, we generated a double mutant (pfl1-1 adh1) that is unable to synthesize both formate and ethanol. (montana.edu)
  • Whereas wild-type Chlamydomonas fermentation primarily produces formate and ethanol, the double mutant reroutes glycolytic carbon to lactate and glycerol. (montana.edu)
  • Higher H 2 yields were associated with fermentation at acidic pH as a consequence of the lower synthesis of other reduced by-products such as formate and ethanol. (biomedcentral.com)
  • At high P H2 the cellular redox state was balanced by rerouting the flow of carbon and electrons to ethanol and formate production allowing unaltered glycolytic flux and growth rate, but resulting in a decreased H 2 synthesis. (biomedcentral.com)
  • However, the distribution of the end metabolites can vary depending on the growth conditions allowing the accumulation of significant amounts of ethanol and formate with consequent reduction of H 2 yield. (biomedcentral.com)
  • In these environments, syntrophy is driven by the exchange of hydrogen and formate in tightly coupled mutualistic interaction among the cooperating microorganisms. (ucla.edu)
  • In addition, the presence of the enzymatic repertoire for formate and hydrogen oxidation in the Rifle clades A and B comammox genomes, respectively, suggest a broader distribution of these metabolic features than previously anticipated. (osti.gov)
  • Effect of histidine-tag and R457H and E580Q mutations on catalytic activity of recombinant human cytochrome P450 oxidoreductase. (moleculardepot.com)
  • In the absence of electron acceptor, downregulation of F 420 H 2 dependent steps of the acetyl-CoA pathway is linked to transient formate generation. (hindawi.com)
  • Growth of E. coli on formate and methanol via the reductive glycine pathway. (mpg.de)
  • Enzymatic activities of Nrf2 target genes, including glutathione S-transferase (GST) and NAD(P)H:quinone oxidoreductase 1 (NQO1), increased at 24 and 48 h after the treatment. (cdc.gov)
  • Beside utilizing CO, A. fulgidus grows with H 2 and formate and a wide variety of simple and complex organic compounds [ 4 - 7 ]. (hindawi.com)
  • quinone oxidoreductase complex (Fqo) is crucial in energy conservation in A. fulgidus during growth with lactate [ 13 ]. (hindawi.com)
  • menaquinone oxidoreductase reaction, as the major mode of energy conservation, rather than formate or H 2 cycling during respiratory growth. (hindawi.com)
  • F 420 oxidoreductase reaction. (hindawi.com)
  • In SRP, three models have been suggested for proton translocation: H 2 cycling, formate cycling, and through "Mitchell-type" loops facilitated by respiratory menaquinone [ 2 , 14 , 15 ]. (hindawi.com)
  • On the other hand, transient formate formation was observed in cultures grown on CO and sulfate [ 2 ], but the enzymatic rationale supporting formate cycling as a mechanism for energy conservation is still lacking. (hindawi.com)
  • Pretreatment of the cells with catalase, but not sodium formate, resulted in a signifi cant inhibitory effect on nano-WC-Co particle-induced Nrf2 target gene activation. (cdc.gov)
  • The active site contains a molecule of beta-mercaptoethanol that is positioned between His-106 and Asp-142 and that appears to mimic the formate product. (rcsb.org)
  • Several proteins that may directly dictate end-product synthesis patterns, including pyruvate:ferredoxin oxidoreductases, alcohol dehydrogenases, and a putative bifurcating hydrogenase, demonstrated differential expression during transition from exponential to stationary phase. (biomedcentral.com)
  • In vivo selection for formate dehydrogenases with high efficiency and specificity towards NADP+. (mpg.de)
  • pyruvate formate-lyase 1 [Ensembl]. (ntu.edu.sg)
  • Glycine radical, Pyruvate formate lyase-like [Interproscan]. (ntu.edu.sg)
  • An NADP -dependent oxidoreductase that catalyses the conversion of 5,10-methyleneterahydrofolate to 5,10-methenyl-tetrahydrofolate. (bvsalud.org)
  • These bacteria lack the aldolase and glucose-6-phosphate NADP + oxidoreductase enzymes and use an alternative route to metabolize carbohydrates, in which phosphoketolases are key. (biomedcentral.com)
  • The enzymes are active in the fermentation of sugars and other compounds to carbon dioxide and are the key enzymes in obtaining energy when bacteria are grown on formate as the main carbon source. (nih.gov)
  • Encodes a unique electron-transfer flavoprotein:ubiquinone oxidoreductase that is localized to the mitochondrion. (or.jp)
  • The substrates acetaldehyde, propanal, and butanal were used for a two-step enzymatic cascade employing first a carboligase and in a second step an oxidoreductase for the synthesis of 2,3 butanediol, 3,4-hexanediol, and 4,5-octanediol, respectively. (rwth-aachen.de)
  • The POBN/.CH(OH)CH3 radical adduct signal was also diminished by formate or mannitol, indicating competition by so-called 'hydroxyl radical scavengers. (nih.gov)
  • In aqueous medium, several different carboligases and oxidoreductases were screened for their substrate spectrum, activity, and stereoselectivity towards the product, and in case for the oxidoreductases, also for the substrate. (rwth-aachen.de)