Comparisons of mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) and single-nucleotide polymorphism (SNP) analyses based on whole-genome sequencing have proven to be helpful for identifying TB outbreaks elsewhere ( 8 , 9 ). (cdc.gov)
Note that SITVITBovis also contains limited information on mycobacterial interspersed repetitive units-variable number of tandem repeats when available. (oup.com)
the complete genome sequence of Mycobacterium tuberculosis continues to provide an invaluable resource to understand tuberculosis (TB), the leading cause of global infectious disease mortality. (jci.org)
The increasing accessibility of whole-genome sequencing across research and clinical settings has improved our ability to predict antibacterial susceptibility, to track epidemics at the level of individual outbreaks and wider historical trends, to query the efficacy of the bacille Calmette-Guérin (BCG) vaccine, and to uncover targets for novel antitubercular therapeutics. (jci.org)
While the genome sequence was already transformative at the time, the past 25 years of progress have substantially increased its impact on TB taxonomy, drug discovery, resistance mechanisms, epidemiology, vaccine development, and pathogenesis. (jci.org)
Whole-genome sequencing (WGS) of M. tuberculosis and related mycobacteria is now routine, allowing comparisons across time and space. (jci.org)
The third report described Genetic Analysis and isolation from a child's hand with multibacterial synergistic Whole-Genome Sequencing gangrene ( 6 ). (cdc.gov)
In contrast, whole genome sequencing offers sensitive views of the genetic differences between strains ofM. (9lib.org)
tuberculosis strains in whole genome sequencing data. (9lib.org)
Rapid respiratory viral whole genome sequencing (WGS) in a clinical setting can inform real-time outbreak and patient treatment decisions, but the feasibility and clinical utility of influenza A virus (IAV) WGS using Nanopore technology has not been demonstrated. (bvsalud.org)
Like mammalian genomes, the sea urchin genome contains a lot of junk DNA, especially repetitive DNA. (blogspot.com)
By scanning the available genome sequences, Demuth et al. (blogspot.com)
Strains2
However, DNA fingerprinting of 7 M. bovis isolates from a 2013 bovine tuberculosis outbreak indicated minimal homology with strains previously circulating in Panama. (cdc.gov)
Although metagenomic tools exist to classify strains in a metagenomic sample, most tools have been developed for more divergent species, and therefore cannot provide the sensitivity required to disentangle strains within closely related bacterial species such asM. (9lib.org)
Epidemiology1
IAV WGS also informs management of nosocomial outbreaks, though molecular and clinical epidemiology were concordant in this study, limiting the impact on decision-making. (bvsalud.org)
Quantify1
purification of antibodies to bacterial antigens by an immunoadsorbent and a method to quantify their reaction with insoluble bacterial targets. (liverpool.ac.uk)
Mechanisms1
Wrong eukaryote is that the economic novelty notion with lunar sequences will demonstrate the other Mechanisms apparently Supported with border sentence. (be-mindful.de)
Transmission1
IAV WGS revealed two separate clusters on one ward and excluded transmission across different wards with contemporaneous outbreaks. (bvsalud.org)
Laboratory1
Accurate IAV genomes can be generated in 24 h using a Nanopore protocol accessible to any laboratory with SARS-CoV-2 Nanopore sequencing capacity. (bvsalud.org)
Time2
The ongoing SARS-CoV-2 pandemic demonstrates the utility of real-time sequence analysis in monitoring and surveillance of pathogens. (bvsalud.org)
To address this, we developed a real-time analysis pipeline to maximise flow cell performance and optimise sequencing time and costs for any amplicon based sequencing. (bvsalud.org)