Organisms escherichia coli proteins. Medical search. Frequent questions

Genes have been transferred within the same species, across species (creating transgenic organisms), and even across kingdoms. (wikipedia.org)
Diagram of the main genes of the locus of enterocyte effacement (LEE) region and the entero- pathogenic Escherichia coli (EPEC) adherence fac- tor (EAF) plasmid. (cdc.gov)
Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
Proteins whose genes are observed to be correlated in expression, across a large number of experiments. (string-db.org)
Genetic studies have identified six genes whose products comprise the general protein secretion machinery of Escherichia coli. (princeton.edu)
This protein, called DksA, coordinates the expression of numerous genes in response to environmental signals. (infectioncontroltoday.com)
Our work deals primarily with the outer membrane proteins, lambda receptor, the product of the lamB gene, and the major outer membrane (porin) proteins 1a and 1b, products of the ompF and ompC genes. (unibas.ch)
The structural genes for the porin proteins, 1a and 1b, are regulated at the transcriptional level by the ompB locus. (unibas.ch)
The course comprises molecular biology of genes, proteins, multicomponent protein complexes, and other biomolecules that provide structure and perform the organism's functions. (lu.se)
This potential pest management method could be easily adapted to target different genes or similar organisms. (cdc.gov)

Bacteria are the easiest organisms to engineer and have been used for research, food production, industrial protein purification (including drugs), agriculture, and art. (wikipedia.org)
The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
Intimin, a 94-kDa outer membrane protein encoded by the eae gene, is responsible for the intimate adherence between bacteria and enterocyte membranes. (cdc.gov)
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
Tetracyclines inhibit protein synthesis and, therefore, bacterial growth by binding with 30S and possibly 50S ribosomal subunits of susceptible bacteria. (medscape.com)
The findings, reported in the current issue of the journal Cell , suggest that bacteria rely on a key protein in order to properly regulate gene expression -- a process fundamental to cell survival. (infectioncontroltoday.com)
However, DnaA proteins from two Gram-positive bacteria, Bacillus subtilis and Streptomyces lividans , while capable of specifically interacting with the DnaA box sequences at oriV , do not bind stably and fail to induce open complex formation. (sri.com)
Long dsRNAs were produced in Escherichia coli strain HT115 (DE3), and a concentrated suspension of heat-killed dsRNA-containing bacteria in 10% sucrose was offered on cotton balls ad-libitum to adult mosquitoes. (cdc.gov)
Produced by the National Institute of Allergy and Infectious Diseases (NIAID), this digitally-colorized scanning electron micrograph (SEM) depicts numbers of Escherichia coli , rod shaped bacteria, which were grown in a culture, and shown here attached to a coverslip. (cdc.gov)

Cayley S, Lewis BA, Guttman HJ, Record MT Jr. Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. (harvard.edu)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
however, as evidenced by genome sequence analysis, the organism lacks common enzymes required for the de novo synthesis of precursors of PGN, which rationalizes its MurNAc auxotrophy. (karger.com)
This extra peptide has been termed the signal sequence, and it has been proposed that this signal plays a role in the localization of the extracytoplasmic protein. (unibas.ch)
Mutations that alter this sequence prevent export of the lambda receptor protein. (unibas.ch)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

In many laboratories, sorbitol-MacConkey medium is commonly used to screen for the slow sorbitol fermentation phenotype of the most common Shiga toxin-containing strain: O157:H7 ( 5 ), but this process does not address the pathogenic potential of the remaining sorbitol-positive E. coli . (cdc.gov)

An In-Silico approach for structural and functional annotation of hypothetical protein fkv26_15010 of vibrio cholera 01", Uttar Pradesh Journal of Zoology , Print ISSN: 0256-971X. (edu.in)

A genomically recoded Escherichia coli strain that lacks all amber codons and release factor 1 (C321. (northwestern.edu)
A has opened new opportunities in chemical and synthetic biology, this strain has not been optimized for protein production, limiting its utility in widespread industrial and academic applications. (northwestern.edu)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)
Enterohemorrhagic Escherichia coli (EHEC) pathogenicity is usually linked to a Shiga toxin ( 1 , 2 ) and virulence factors, including adhesins, toxins, invasins, protein secretion systems, iron uptake systems, and several unidentified functions ( 3 , 4 ), which are unrelated to strain phylogeny. (cdc.gov)

What is the cellular localization of the protein? (embl.org)
Specificity of localization and phosphotransfer in the CheA proteins of Rhodobacter sphaeroides. (ox.ac.uk)
Genetic analysis of these mutations has provided evidence for the involvement of the ribosome in the process of protein localization. (unibas.ch)
This has permitted us to extend our studies on outer membrane protein localization to protein 1. (unibas.ch)

A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. (wikipedia.org)
A wide variety of organisms have been genetically modified (GM), from animals to plants and microorganisms. (wikipedia.org)
Creating a genetically modified organism is a multi-step process. (wikipedia.org)
Herbert Boyer and Stanley Cohen made the first genetically modified organism in 1973, a bacterium resistant to the antibiotic kanamycin. (wikipedia.org)
The definition of a genetically modified organism (GMO) is not clear and varies widely between countries, international bodies, and other communities. (wikipedia.org)

The recombinant protein was not recognised by antiserum directed against a fibronectin binding treponemal adhesion that contained the same electrophoretic mobility. (bmj.com)
The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)

These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)
There are also eukaryotic relatives of this protein. (nih.gov)
Despite differences in the residue and protein selectivities of prokaryotic and eukaryotic ROXs, comparison of the crystal structures of E. coli YcfD and Rhodothermus marinus YcfD with those of human MINA53 and NO66 reveals highly conserved folds and novel dimerization modes defining a new structural subfamily of 2OG-dependent oxygenases. (ox.ac.uk)
In the last few years, several laboratories have demonstrated that many proteins (both from eukaryotic and prokaryotic organisms) that are destined to be localized in noncytoplasmic locations initially are synthesized as a precursor with a 15-30 amino acid extension at the NH2-terminal end of the molecule. (unibas.ch)

This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. (nih.gov)
such as the amino acids present in cellular proteins, using electric sparks in an atmosphere of gas composed of steam, methane, ammonia and hydrogen. (discoveringislam.org)
A) enables efficient genetic encoding of chemically diverse non-canonical amino acids (ncAAs) into proteins. (northwestern.edu)

The EAF plasmid is not essential for the formation of A/E ducing E. coli (enterohemorrhagic E. coli [EHEC]) and in lesions, although its presence enhances their efficiency, strains of other bacterial species (1). (cdc.gov)
Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. (genome.jp)
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. (cdc.gov)

We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. (nih.gov)
MG1655, Escherichia coli MG1655, Escherichia coli str. (string-db.org)

And I knew that Bacillus subtilis was an organism that could accept DNA. (webofstories.com)
Why is Bacillus subtilis respiratory Complex II not assembled when produced in Escherichia coli? (lu.se)
The experimental part focus on the model organisms, Bacillus subtilis , Escherichia coli and Streptomyces coelicolor . (lu.se)

We describe the construction and characterization of a genomically recoded organism (GRO). (nih.gov)

In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)
Carbapenems inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
In contrast to a chromosomal replication origin, which functionally interacts only with the native DnaA protein of the organism, the ability of RK2 to replicate in a wide range of Gram-negative bacterial hosts requires the interaction of oriV with many different DnaA proteins. (sri.com)
1. Bacterial resistance to beta-lactam antibiotics has risen dramatically in Escherichia coli from food animals. (who.int)

Protein aggregation remains a significant problem in E. coli expression systems. (iucr.org)
An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. (string-db.org)

Studies of antigenic varia- tions in the 280-amino acid residues of the C-terminal portion of intimin (the receptor-binding domain of the protein) and the use of polymerase chain reaction analysis allow the classifica- tion of distinct intimin types or subtypes among EPEC and STEC strains (6). (cdc.gov)
The N-terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS-CoV NP-NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. (iucr.org)

stx 1, stx 2, eae , and hlyA (or ehx A). Variation in amplification success is likely to increase because more virulence factor variants are certain to emerge as more EHEC and Shiga toxin-producing E. coli (STEC) strains are identified. (cdc.gov)

ROX structures with and without their substrates support their functional assignments as hydroxylases but not demethylases, and reveal how the subfamily has evolved to catalyse the hydroxylation of different residue side chains of ribosomal proteins. (ox.ac.uk)

Uropathogenic Escherichia coli (UPEC) is responsible for the majority of urinary tract infections (UTI). (pasteur.fr)

Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
In mammals as in Escherichia coli, the enzyme consists of two proteins. (hal.science)
The lac operon of E. coli is but one example of how enzyme and transporter production can be made conditional on the presence of a nutrient to catabolize. (lu.se)
The initial velocity decreased at enzyme concentrations higher than 0.13 mg protein per 2.0 ml of incubation mixture. (who.int)

Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
So triosephosphate isomerase from me and E. coli [ Escherichia coli] and yeast and lobsters is all a very similar protein. (webofstories.com)
The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. (nih.gov)
In unicellular organisms like E. coli and yeast, the benefits of between potential cost and benefit, regulation can make the most a well-adapted regulatory system are readily quantified, as the difference to the long-term growth rate if the resource in question fitness of an individual can be estimated by its growth rate in is only available a similarly small fraction of the time. (lu.se)

Quinolones have broad activity against gram-positive and gram-negative aerobic organisms. (medscape.com)
Staphylococcal Infections Staphylococci are gram-positive aerobic organisms. (msdmanuals.com)

Initiation of chromosomal replication and its cell cycle-coordinated regulation bear crucial and fundamental mechanisms in most cellular organisms. (elsevierpure.com)

In case expression constructs and/or protocols are already present, you can immediately fill out our protein expression and purification request form and send the completed form back to us. (embl.org)
Once suitable conditions have been identified, we proceed to large scale expression (1-6 liter per construct) and protein purification using a variety of chromatographic techniques. (embl.org)
After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
Here we report the cloning, expression, purification and characterization of protein R1 from Arabidopsis thaliana. (hal.science)

Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
This study provides a molecular explanation for MTERF4-dependent recruitment of NSUN4 to ribosomal RNA and suggests a unique mechanism by which other members of the large MTERF-family of proteins can regulate ribosomal biogenesis. (rcsb.org)

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane. (string-db.org)
Bieker, KL & Silhavy, TJ 1990, ' The genetics of protein secretion in E. coli ', Trends in Genetics , vol. 6, no. (princeton.edu)

Escherichia coli DnaA protein forms a homomultimeric complex with the replication origin (oriC). (elsevierpure.com)
Whereas DnaA V211A and R245A proteins retained normal affinities for ATP/ADP and DNA and activity for the ATP-specific conformational change of the initiation complex in vitro, oriC complexes of these mutant proteins were inactive in DUE unwinding and in binding to the single-stranded DUE. (elsevierpure.com)
Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin ( oriV ). (sri.com)
In this study we compared the interactions of oriV with five different DnaA proteins. (sri.com)
DNase I footprint, gel mobility shift, and surface plasmon resonance analyses showed that the DnaA proteins from Escherichia coli , Pseudomonas putida , and Pseudomonas aeruginosa bind to the DnaA boxes at oriV and are capable of inducing open complex formation, the first step in the replication initiation process. (sri.com)
These results suggest that the inability of the DnaA protein of a host bacterium to form a stable and functional complex with the DnaA boxes at oriV is a limiting step for plasmid host range. (sri.com)

Implications for protein-DNA interactions in vivo. (harvard.edu)
Crowding and confinement effects on protein diffusion in vivo. (harvard.edu)
In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). (natx-ray.com)

The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. (nih.gov)
Ampicillin is a second-generation penicillin that is active against many strains of Escherichia coli, Proteus mirabilis, Salmonella, Shigella, and Haemophilus influenzae. (medscape.com)

the organism lacks the pathway. (lbl.gov)

Mammals are the best model organisms for humans, making ones genetically engineered to resemble serious human diseases important to the discovery and development of treatments. (wikipedia.org)

Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. (iucr.org)
There is extensive literature on the variables leading to insoluble recombinant expression of proteins. (iucr.org)
The authors envision that their library of organisms will provide the community with multiple options for increased expression of proteins with new and diverse chemistries. (northwestern.edu)

CDI+ strains produce cell floor CdiA effector proteins that bind particular receptors on neighboring micro organism to provoke toxin supply. (natx-ray.com)

Atypical EPEC is more closely related to Shiga toxin-producing E. coli (STEC), and like STEC these strains appear to be emerging pathogens. (cdc.gov)
3) Minimum dose test - consists of identifying the lowest amount of active principle (protein substrate) necessary to obtain the antibiotic action against pathogens. (societyforscience.org)
Applying these methods, the results indicated positive antimicrobial action by the protein substrate after 48 hours of exposure of the pathogens to proteins. (societyforscience.org)

Substrate protein folds while it is bound to chaperone. (string-db.org)
Finally, in the dose test was found that a minimum concentration of 3% of protein substrate was necessary to verify their antibiotic action. (societyforscience.org)
Protein R1 is the proper reductase as it contains, in the substrate binding site, the reducing active cysteine pair. (hal.science)
The structures reveal that new protein hydroxylation activities can evolve by changing the coordination position from which the iron-bound substrate-oxidizing species reacts. (ox.ac.uk)

The chemosensory pathway of Rhodobacter sphaeroides comprises multiple homologues of chemotaxis proteins characterized in organisms such as Escherichia coli. (ox.ac.uk)

Expression in E. coli was made possible by coexpression of tRNAArg4 which is required for the utilization of AGA and AGG as codons for arginines. (hal.science)

SQR consists of a membrane-peripheral heterodimer domain (often called succinate dehydrogenase) which is tightly bound to a membrane-spanning anchor, which is a cytochrome with one or two heme groups depending on the organism. (lu.se)
The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)

Compared with imipenem, meropenem has slightly increased activity against gram-negative organisms and slightly decreased activity against staphylococci and streptococci. (medscape.com)
Contact-dependent progress inhibition (CDI) methods allow the direct switch of protein toxins between competing Gram-negative micro organism. (natx-ray.com)

Proteins crucial for the respiratory chain are translated by the mitochondrial ribosome. (rcsb.org)
It is indicated for the treatment of infections caused by susceptible organisms involving the respiratory tract. (medscape.com)
It is indicated for the prophylaxis or treatment of mild to moderately severe upper respiratory tract infections caused by organisms susceptible to low concentrations of penicillin G. (medscape.com)

Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)

A cross reactive molecule like P2 was not synthesised by the avirulent spirochaete, T phagedenis biotype Reiter, which indicated that P2 is a protein specific to virulent T pallidum organisms. (bmj.com)

The picture emerging provides a useful paradigm for similar pathways in other organisms. (princeton.edu)

Even then, this comparison is very weak, for the actual structural complexity of an elementary living organism is much more complex than the structure of the Eiffel Tower, considered in 1889 to be a triumph of metal construction. (discoveringislam.org)
The finding that ribosomal oxygenases (ROXs) occur in organisms ranging from prokaryotes to humans raises questions as to their structural and evolutionary relationships. (ox.ac.uk)
The virion envelope surrounding the nucleocapsid contains the following structural proteins: S (spike), M (matrix), E (envelope) and N (nucleocapsid). (iucr.org)

It is demonstrated that the functional deactivation of nucleases (e.g., rne, endA) and proteases (e.g., lon) increases production of wild-type superfolder green fluorescent protein (sfGFP) and sfGFP containing two ncAAs up to ≈5-fold. (northwestern.edu)
each protein has a subset of the CheA domains, with CheA(3) phosphorylating CheA(4) together making a functional CheA protein. (ox.ac.uk)
It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)

Lengthy non-coding RNAs (lncRNAs) have been outlined as transcripts longer than 200 nucleotides, which lack vital protein coding potential and possess essential roles in numerous mobile processes. (natx-ray.com)
Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management. (cdc.gov)

One recently characterized AT protein of UPEC is UpaH, a large adhesin-involved-in-diffuse-adherence (AIDA-I)-type AT protein that contributes to biofilm formation and bladder colonization. (pasteur.fr)

In contrast, all of the UpaH variants examined retained a conserved ability to mediate binding to extracellular matrix (ECM) proteins. (pasteur.fr)

Anatomy8

Organisms24

Diseases7

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes79

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Enterohemorrhagic3

MG16552

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Aggregation2

Residues2

EHEC1

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Purification4

Molecular2

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DnaA6

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Extracellular1