SolventHPLCStationary phaseCompoundsSolventsChromatographic techniquesPurifySeparationSilicaLiquidExtractionAgarosePolaritySoluteProteinsMixturesMoleculeVaporAqueousInorganicPurityComplex mixtureSynthesisACIDPaperChemicalsColumnSubstancesAnalysisPesticidesAmino acidsChemistry studentsMobileTissuesMethodsTypeMigrationSamples
Solvent11
- Paper chromatography is when it separates liquid samples with a liquid solvent and a paper strip. (majortests.com)
- Separation of dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of silica gel. (majortests.com)
- The thin layer chromatography plate is out into a beaker containing the solvent but making sure that the solvent is below the pencil line. (majortests.com)
- Eluent is a fancy name for solvent in chromatography. (stackexchange.com)
- Flash Column chromatography is a form of liquid chromatography that uses a solvent coupled with a pressurized gas like nitrogen in order to aid in the separation process . (extraktlab.com)
- Chromatography relies on two different 'phases': the mobile phase is the solvent that moves through the paper, carrying different substances with it. (profound-answers.com)
- Water dielectric constant is reduced from 85 at 25°C to 35 at 200°C cause that water behave like an organic solvent. (profound-answers.com)
- The molecule phase displays the solvent used in chromatography. (profound-answers.com)
- In paper and thin-layer chromatography the mobile phase is the solvent. (profound-answers.com)
- Lipopeptides in solvent extracts were quantified by thin layer chromatography (TLC) which showed methanol to be the best solvent which extracted 100% of lipopeptides with a purity of 74% for fengycin. (sun.ac.za)
- Further analysis of the lipopeptide solvent extract by liquid chromatography electrospray ionisation mass spectroscopy (LC-ESI-MS) was conducted to identify the two bands that appeared on the plate when the solvent extracts were analysed by TLC, and which were assumed to be fengycin. (sun.ac.za)
HPLC6
- Paper, thin-layer, and classical column chromatography techniques, as well as high-performance liquid chromatography (HPLC) and ion chromatography, all belong to the class of liquid chromatography. (edu.au)
- High-performance liquid chromatography or HPLC is a form of column chromatography that is most frequently used in analytical chemistry . (extraktlab.com)
- The name "high-performance" and "high-pressure" are often used interchangeably because of the relatively high pressures used in HPLC chromatography systems. (extraktlab.com)
- This Chromatography method is very similar to HPLC chromatography, but does not operate under the same high pressures as HPLC. (extraktlab.com)
- Similar to an HPLC chromatography system, supercritical fluid chromatography uses high pressure fluid in a supercritical state as the mobile phase . (extraktlab.com)
- Reverse-phase high performance liquid chromatography (RP-HPLC) quantification of acid precipitate showed high recoveries of 78% and 62% for fengycin and iturin respectively at pH 2 with an optimal fengycin purity of 64% obtained at pH 3. (sun.ac.za)
Stationary phase11
- The silica gel is the stationary phase, TLC uses a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
- Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
- It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
- The stationary phase is a minute layer of liquid inside a tubing called a column. (extraktlab.com)
- The stationary phase of this chromatography type uses a thin layer (hence the name) of adsorbent material like a silica gel or aluminum oxide on what is called a carrier sheet. (extraktlab.com)
- This form of chromatography uses two solvents and paper as a stationary phase. (extraktlab.com)
- Very simply, the stationary phase of chromatography is the phase that the solution that is suspended in a mobile phase passes through in order to perform separation . (extraktlab.com)
- The mobile phase in chromatography is the medium in which the solution being tested or separated is introduced and passes through the stationary phase. (extraktlab.com)
- chemistry) The liquid or gas that flows through a chromatography system, moving the materials to be separated at different rates over the stationary phase. (profound-answers.com)
- What do you mean by mobile phase and stationary phase in chromatography? (profound-answers.com)
- In all chromatography there is a mobile phase and a stationary phase. (profound-answers.com)
Compounds9
- This is used to identify organic and inorganic compounds and is used in crime scene investigation. (majortests.com)
- This is used to test water pollution and can be used to analyse metal ions and organic compounds in solutions. (majortests.com)
- Odorous or volatile compounds released from various biological fluids are determined and identified by gas chromatography-mass spectrometry. (vetmeduni.ac.at)
- A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
- Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. (lookformedical.com)
- A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
- Gas chromatography is frequently used in pharmaceuticals, cosmetics, and forensics due to the ability to operate with volatile compounds. (extraktlab.com)
- Thin-Layer chromatography is a common chromatography method most often used for the separation of organic compounds. (extraktlab.com)
- Because this is such a simple form of chromatography, it is ideal for studies that are limited in resources and is a great method for testing cannabinoids and other plant compounds. (extraktlab.com)
Solvents4
- For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)
- Mobile phase (moving phase): organic solvents - Important properties: polarity. (profound-answers.com)
- Organic solvents of varying polarity indices were screened for their efficiency in extracting fengycin and iturin. (sun.ac.za)
- The extraction efficiency of fengycin by the organic solvents was found to be related the functional groups of the solvents, in addition to the polarity. (sun.ac.za)
Chromatographic techniques1
- This article gives an overview of classical, high-performance, and thin-layer chromatographic techniques including experimental configurations and forensic applications. (edu.au)
Purify4
- Chromatography is the separation of mixtures into their components as a result of differential distribution of the solutes in order for identification, analyses, purify and/or quantity of the mixture for a mobile medium and for a stationary absorbing medium. (majortests.com)
- Studies have indicated the inhalation of aflatoxin contaminated dust by chemical engineers working with contaminated peanut dust and biochemists working to purify aflatoxins by preparative thin layer chromatography. (cdc.gov)
- 2. Purify an organic compound by Thin Layer Chromatography. (edu.pk)
- Chromatography is a key method used in biotechnology processing to isolate proteins from culture broths for example and purify them. (hs-esslingen.de)
Separation4
- What is the basic principle for separation in normal phase, reversed phase, ion-exchange and gel permeation chromatography? (majortests.com)
- chromatography: principles and applications of partition and adsorption as applied to separation of samples using paper and thin layer chromatography. (kent.ac.uk)
- A discussion of these separation mechanisms can be found in Chromatography: Basic Principles. (edu.au)
- Separation of different color pigments by Paper Chromatography. (edu.pk)
Silica1
- Thin layer chromatography is done using a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
Liquid4
- Liquid chromatography is a broad classification used to describe a variety of different chromatographic configurations that rely on the use of a liquid mobile phase. (edu.au)
- Pure water in elevated temperature can be used as mobile phase in liquid chromatography mainly due to change of dielectric constant. (profound-answers.com)
- Which is the mobile phase of liquid chromatography? (profound-answers.com)
- With liquid-liquid extraction , students become familiar with a further method to separate organic active substances from a culture broth. (hs-esslingen.de)
Extraction5
- There are varying types of chromatography that are used throughout a number of industries including the hemp and cannabis extraction industry. (extraktlab.com)
- As it is used for testing, and separations, the types of chromatography used in the extraction industry are becoming essential to ensure a safe and effective product. (extraktlab.com)
- In this article, we will walk through some of those chromatography methods and where they are used in the extraction industry . (extraktlab.com)
- Those pressures allow for high efficiency and throughput making it a very reputable chromatography method for larger scale extraction operations. (extraktlab.com)
- During the experimental days preparative methods like extraction of lipids from brain and liver, alkoholic DNA precipitation, dialysis and chromatography of proteins and isolation of antibodies used. (hs-esslingen.de)
Agarose1
- Furthermore analytical methods like SDS-PAGE, thin layer chromatography, Agarose gel electrophoresis, ELISA and turbidimetry, enzymatic analytic, Bradford protein assay , Biuret protein assay and BCA assay are used. (hs-esslingen.de)
Polarity2
- Normal and Reversed Phase Chromatography What is the relationship between polarity of the mobile phase vs. elution time and resolution in normal phase and reversed phase chromatography? (majortests.com)
- How does the polarity of the eluent and sample affect the Rf value in thin layer chromatography? (stackexchange.com)
Solute2
- Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
- What factors determine the rate of migration of a solute in paper chromatography? (profound-answers.com)
Proteins1
- Doc Brown's Chemistry Advanced Level Pre-University Chemistry Revision Study Notes for GCE IB advanced A level organic chemistry students US K12 grade 11 grade 12 organic chemistry the chemistry of amino acids structure and reactions 20 which amino acids are in proteins? (docbrown.info)
Mixtures2
- Task 2 Chromatography analysis: Thin layer chromatography Chromatography is used to separate mixtures of substances in to components. (majortests.com)
- Paper chromatography is a chromatography technique for separating and testing mixtures using pigment or color. (extraktlab.com)
Molecule3
- Occasionally, on the DAT Organic Chemistry section, you'll be asked about one of the chemical lab tests you likely used in your lab sections to identify the functional groups in a molecule. (bootcamp.com)
- The addition of an organic acid radical into a molecule. (lookformedical.com)
- What role does the mobile phase play in the distance a molecule travels in chromatography? (profound-answers.com)
Vapor1
- A NIOSH-certified CBRN PAPR with a loose-fitting face-piece, hood, or helmet and a filter or a combination organic vapor, acid gas, and particulate cartridge/filter combination or a continuous flow respirator for air levels greater than AEGL-1. (cdc.gov)
Aqueous1
- The combined organic fractions are washed with saturated aqueous solution of NH 4 Cl (3 x 75 mL), and dried over Na 2 SO 4 (15 g). (orgsyn.org)
Inorganic1
- The G:T ratio of animals fed the "normal" diet which contained 0.10 percent inorganic sulfur and 0.57 percent organic sulfur was the smallest indicating the highest relative conjugation with taurine. (tennessee.edu)
Purity1
- The mixture is then subjected to thin layer chromatography (TLC) to monitor the purity and to separate different GSLs. (springer.com)
Complex mixture1
- This form of chromatography is used most often for separating chemicals in a complex mixture. (extraktlab.com)
Synthesis1
- MCBF also houses microwave-based peptide synthesizer along with organic synthesis module and ultracentrifuge provided with three rotors for varied applications. (iitgn.ac.in)
ACID2
- Secrets Of Tea Catnip Colic Tea Colic Reliever, Gas, Acid Reflux Relief - Natural USDA Organic. (johnharvards.com)
- Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. (lookformedical.com)
Paper1
- This is also commonly referred to as two-way paper or two-dimensional chromatography. (extraktlab.com)
Chemicals1
- Examples of when chromatography is used are in pharmaceutical companies in order to find out the amount of chemicals used in each new product, hospitals for detection of sugar/blood levels and law enforcement for comparisons of samples found at a crime scene. (majortests.com)
Column1
- What makes components move faster in column chromatography? (profound-answers.com)
Substances1
- Gas chromatography-mass spectrometry, thin-layer chromatography, atomic absorption spectrometry, etc. are employed to monitor these substances. (vetmeduni.ac.at)
Analysis1
- Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis were used to profile volatile organic metabolites (VOCs) present in the extracts. (bvsalud.org)
Pesticides1
- Thin-layer chromatography is used to detect pesticides or insecticide in food. (majortests.com)
Amino acids1
- The general formula for alpha amino acids is R-CH(NH 2 )-COOH, where R can be H or a wide variety of organic groups. (docbrown.info)
Chemistry students1
- Techniques in Organic Chemistry is the most comprehensive and detailed presentation of lab techniques available for organic chemistry students - and the least expensive. (stanford.edu)
Mobile3
- In gas chromatography, the mobile phase used is in the form of an inert gas such as helium or nitrogen . (extraktlab.com)
- What does the mobile phase do in chromatography? (profound-answers.com)
- What makes a good mobile phase in chromatography? (profound-answers.com)
Tissues1
- Fatty acids from animal tissues and foods are determined by gas chromatography and gas chromatography-mass spectrometry. (vetmeduni.ac.at)
Methods2
- The article will briefly talk about the basics of chromatography, five of the most common types of chromatography used in the industry, and which methods are best for their intended purposes in hemp and cannabis processing. (extraktlab.com)
- There are nearly a dozen commonly used methods of chromatography , but some are more common than others. (extraktlab.com)
Type1
- A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (lookformedical.com)
Migration1
- Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (lookformedical.com)
Samples1
- The excretion of 14C-labelled test substance was studied using collected samples of urine, faeces and bile by thin layer chromatography and radioassay. (europa.eu)