Optics microscopy confocal. Medical search. Frequent questions

Light travels through the sample under a conventional microscope as far into the specimen as it can penetrate, while a confocal microscope only focuses a smaller beam of light at one narrow depth level at a time. (wikipedia.org)
In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal - the name "confocal" stems from this configuration. (wikipedia.org)
Carl Zeiss - The LSM 510 laser scanning confocal microscope offered by Zeiss can collect data in up to four channels simultaneously utilizing a quad-photomultiplier 12-bit A/D detection system. (fsu.edu)
Illumina: Confocal Microscopy - Molecular Dynamics, a subsidiary of GE Healthcare (formerly Amersham Bioscience), is a manufacturer of confocal microscope systems, image analysis software, volume rendering and three-dimensional modeling tools. (fsu.edu)
Nikon Confocal Microscopy - Nikon's PCM 2000 biosciences confocal microscope employs twin photomultiplier tubes with options for single or triple configurations. (fsu.edu)
Olympus America - The Olympus FluoView is a point-scanning, point-detection, confocal laser scanning microscope for biology research applications. (fsu.edu)
Optiscan - The Optiscan Personal Confocal microscope offers a twin detection system utilizing both argon-ion and krypton-argon lasers for simultaneous fluorescence imaging. (fsu.edu)
The microscope is controlled by the Optiscan Confocal Control software and images can be analyzed with Optiscan Analyze, a Windows-based program. (fsu.edu)
In the traditional fluorescence microscope configuration, including many confocal systems, spectral filtering of both excitation and emission light is accomplished utilizing thin-film interference filters. (olympusconfocal.com)
Even though resolution is somewhat enhanced with confocal microscopy over conventional widefield techniques, it is still considerably less than that of the transmission electron microscope. (olympusconfocal.com)
When imaged with a laser scanning confocal microscope (Figure 1(d)), the medulla thick section reveals a significant degree of structural detail. (olympusconfocal.com)
Following Minsky's work, M. David Egger and Mojmir Petran fabricated a multiple-beam confocal microscope in the late 1960s that utilized a spinning (Nipkow) disk for examining unstained brain sections and ganglion cells. (olympusconfocal.com)
Continuing in this arena, Egger went on to develop the first mechanically scanned confocal laser microscope, and published the first recognizable images of cells in 1973. (olympusconfocal.com)
Fortuitously, shortly after Minsky's patent had expired, practical laser scanning confocal microscope designs were translated into working instruments by several investigators. (olympusconfocal.com)
Dutch physicist G. Fred Brakenhoff developed a scanning confocal microscope in 1979, while almost simultaneously, Colin Sheppard contributed to the technique with a theory of image formation. (olympusconfocal.com)
The confocal Raman instrument used in this experiment is based on an inverted optical microscope equipped with an x,y-scan stage. (rhk-tech.com)
The 2fFCS set-up is based on a specificly modified MicroTime 200 confocal microscope. (picoquant.com)
A sample scanning stage enables easy and rapid alignment of the sample relative to the illumination source and ensures that the microscope optics are independent of the AFM scanner. (nanonics.co.il)
Fluorescence confocal laser scanning endomicroscopy is a novel tool combining confocal microscopy and endoscopy for in-vivo subcellular structure imaging with comparable resolution as the traditional microscope. (jpier.org)
Fitted with a back-illuminated scientific CMOS camera provided by Photometrics, the Re-scan Confocal Microscope (RCM) improves resolution with an optical "trick", by first scanning the microscope image and then re-scanning it onto the camera chip using a pair of mirrors. (optics.org)
The RCM technique is based around a box containing pre-aligned optics that Photometrics says can be connected to any microscope, to convert it from a confocal system to one capable of imaging beyond Abbe's classical diffraction limit. (optics.org)
In a blog post last month, Confocal.nl claimed that the RCM represented the "first commercial-optics-only super-resolution confocal microscope" on the market. (optics.org)
RCM is a standard confocal microscope extended with a detection unit, where a pair of scanning mirrors project the emission light directly onto a camera in a scanning manner," wrote co-founder Manders. (optics.org)
The versatile ZEISS Smartproof 5 widefield confocal microscope is your integrated system for surface analysis: fast, precise and repeatable. (zeiss.com)
Benefit from a fully integrated system: optics, electronics and camera are all enclosed in the microscope. (zeiss.com)

The principle of confocal imaging was patented in 1957 by Marvin Minsky and aims to overcome some limitations of traditional wide-field fluorescence microscopes. (wikipedia.org)
Confocal microscopes work on the principle of point excitation in the specimen (diffraction limited spot) and point detection of the resulting fluorescent signal. (wikipedia.org)
Four types of confocal microscopes are commercially available: Confocal laser scanning microscopes use multiple mirrors (typically 2 or 3 scanning linearly along the x- and the y- axes) to scan the laser across the sample and "descan" the image across a fixed pinhole and detector. (wikipedia.org)
Spinning-disk (Nipkow disk) confocal microscopes use a series of moving pinholes on a disc to scan spots of light. (wikipedia.org)
The Zeiss confocal website offers technical and application information, as well as a gallery of digital images acquired with these microscopes. (fsu.edu)
Leica Microsystems Confocal Microscopy - Leica's confocal microscopy website provides introductory material about confocal microscopy, tutorials and manuals about Leica microscopes, FAQ's, application notes, a publications listing, and an image gallery. (fsu.edu)
Resolution is not clearly defined with full-field instruments that derive surface height data from a sequence of tens or even hundreds of digitized camera frames, as is the case with coherence and confocal scanning interference microscopes. (azooptics.com)
When it became available for biological microscopes, researchers let manufacturers know what they needed so as to use this new science consistently, and manufacturers responded with special optics, lighting, highly accurate focus capability, sensitive cameras and software. (photonics.com)
The widefield confocal technology of Smartproof 5 offers greater throughput compared to classical point scanning confocal microscopes. (zeiss.com)
This is achieved by the correlated aperture spinning disc technology integrated in the Smartproof 5 detector module, while maintaining the typical benefits of confocal microscopes for surface characterization. (zeiss.com)

Combining confocal microscopy, direct STochastic Optical Reconstruction Microscopy (dSTORM), and proteomic analysis, we were able to carry out a complete analysis of the PC formation and evolution. (tue.nl)

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sections from thick specimens. (olympusconfocal.com)
Presented in Figure 1 are a series of images that compare selected viewfields in traditional widefield and laser scanning confocal fluorescence microscopy. (olympusconfocal.com)
Likewise, widefield fluorescence imaging of whole rabbit muscle fibers stained with fluorescein produce blurred images (Figure 1(b)) lacking in detail, while the same specimen field (Figure 1(e)) reveals a highly striated topography in confocal microscopy. (olympusconfocal.com)
Because of its patented widefield, correlated aperture confocal technology, your Smartproof 5 minimizes the time to result thus providing a perfect balance between high resolution and high speed. (zeiss.com)
The new ZEISS objective class C Epiplan-Apochromat has been especially designed for widefield confocal systems. (zeiss.com)
These high numerical aperture lenses not only produce the best performance in the visible range, but also at 405 nm - the wavelength used for the acquisition of widefield confocal images. (zeiss.com)

While the confocal setup does not deliver the kind of state-of-the-art resolving power of more advanced approaches like stimulated emission depletion (STED) fluorescence microscopy and related techniques, it is said to deliver axial resolutions as low as 170 nm. (optics.org)
Octopus offers a range of imaging techniques including several modes of multidimensional single molecule microscopy (particle kinetics and dynamics in live cells via single particle tracking colocalisation, single particle FRET and single particle polarisation, and structure determination in fixed cells - at ~5 nm resolution via fluorescence localisation with photobleaching (FLimP)), super-resolution microscopy (STORM, PALM, SIM, STED), and confocal microscopy (FLIM, FRET, and multiphoton). (wayforlight.eu)
To this aim, we use STED microscopy, phase holography microscopy, flow cytometry, immunocytochemistry and electron microscopy techniques to investigate the phenotype of a variety of cells cultured on nanowires and nanotubes. (lu.se)
In order to deepen our understanding of the interactions between the cell membrane and nanostructures, we perform STED microscopy live imaging of the membrane of cells cultured on nanostructures. (lu.se)

Also presented in a nicely-executed format is information about video-enhanced light microscopy, scanning probe microscopy, electron microscopy, electron diffraction, and digital imaging. (fsu.edu)

spectroscopy of nanostructures including confocal Raman microscopy, fluorescence, linear and nonlinear optics, integrated optics spectroscopy of thin films and interfaces. (internano.org)

Participants underwent imaging with confocal scanning laser tomography for measurement of DM rim area (DM-RA) and with spectral domain optical coherence tomography (SD OCT) for quantification of a BMO-based neuroretinal rim parameter, minimum rim width (BMO-MRW), and RNFL thickness. (nih.gov)

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. (wikipedia.org)
Andor - Andor Technology offers a range of confocal and live cell imaging systems through their Microscopy Systems Division. (fsu.edu)
In addition to product information, the Andor site includes in its library a section on confocal imaging that provides an excellent concise introduction to basic confocal concepts and equipment. (fsu.edu)
Confocal Imaging - Developed by the Laboratory for Optical and Computational Instrumentation at the University of Wisconsin, this website provides an introduction to confocal imaging and a variety of related topics. (fsu.edu)
From Bones to Atoms: Imaging Nature Across Dimensions - Written by scientists from the M.E. M ller Institute for Microscopy at the University of Basel (Switzerland), this website offers introductory information about optical sectioning using confocal microscopy techniques. (fsu.edu)
We demonstrate the modalities of bright- and dark-field imaging and scanning fluorescence microscopy at acquisition rates that allow observation of dynamic processes such as Brownian motion of mesoscopic particles. (nature.com)
Even though optical microscopy is limited in lateral and axial resolution to limits of approximately 200 and 500 nanometers, respectively, recent breakthroughs have resulted in the introduction of superresolution imaging techniques that have broken this diffraction limit. (microscopyu.com)
There has been a tremendous explosion in the popularity of confocal microscopy in recent years, due in part to the relative ease with which extremely high-quality images can be obtained from specimens prepared for conventional fluorescence microscopy, and the growing number of applications in cell biology that rely on imaging both fixed and living cells and tissues. (olympusconfocal.com)
Tony Wilson, Brad Amos, and John White nurtured the concept and later (during the late 1980s) demonstrated the utility of confocal imaging in the examination of fluorescent biological specimens. (olympusconfocal.com)
Imaging silicon atoms with terahertz-driven scanning tunnelling microscopy (TD-STM). (rhk-tech.com)
This course will provide the participants with an overview about the basics of microscopy (light and optics, contrast techniques and imaging). (conference-service.com)
The confocal part will include multicolor, 3D and time lapse imaging as well as spectral imaging, photobleaching (FRAP, FLIP, FLAP) and photoactivation techniques. (conference-service.com)
Additionally, Carl Zeiss has signed collaboration and development agreements with Cornell and with multiphoton microscopy co-inventor Watt Webb, director of the National Institutes of Health-funded Developmental Resource for Biophysical Imaging and Opto-electronics. (laserfocusworld.com)
Soft Imaging is a provider of software and hardware solutions for digital imaging that has worked with Olympus for many years in the light-optical microscopy market. (laserfocusworld.com)
The central aim of this research is to overcome the imaging depth limit in biological microscopy. (jobs.ac.uk)
This multi-disciplinary project fuses computational imaging and adaptive optics in microscopy , as well as the further development of existing systems with a range of modalities such as light-sheet microscopy, structured illumination, phase contrast, and confocal microscopy. (jobs.ac.uk)
The immunoreactivity levels of well-characterized antibodies, for all major retinal cells and their compartments, were obtained using our established semiquantitative confocal and imaging techniques. (molvis.org)
Analytical imaging and Microscopy 3. (iiconservation.org)
Nanonics has designed The MultiView 2000 for excellence in scanning probe microscopy while allowing for near-field and far-field optical NSOM/AFM Raman/TERS imaging without perturbation. (nanonics.co.il)
The large, 85μ X, Y and Z-range of the Nanonics 3D FlatscanTM makes it ideal for optical sectioning in confocal imaging. (nanonics.co.il)
Used in this way, the MultiView 2000 integrates conventional far-field imaging, confocal microscopy, AFM, and near-field optics in a single system. (nanonics.co.il)
Although microscopy techniques used for calcium imaging have generally a small field of view, complete transparency over larger areas is critical to use them with wide-field voltage imaging or in serial calcium imaging experiments to correlate small-scale and large-scale dynamic networks. (nature.com)
Yonghong Shao, "Multi-Laser Scanning Confocal Fluorescent Endoscopy Scheme for Subcellular Imaging (Invited)," Progress In Electromagnetics Research , Vol. 169, 17-23, 2020. (jpier.org)
Described as a cost-effective solution for researchers wanting to get beyond the diffraction limit without blowing their laboratory budgets, the RCM requires a lower laser power compared to conventional confocal microscopy, making it better suited to imaging live cells. (optics.org)
With his team, he has made numerous advances in adaptive aberration correction for microscopy, with applications ranging from super-resolution cell imaging to deep-tissue imaging for neuroscience. (optica.org)
The OCTOPUS (Optics Clustered to OutPut Unique Solutions) imaging cluster offers a range of advanced imaging techniques including multidimensional single molecule microscopy, multiphoton, confocal microscopy and optical tweezers. (wayforlight.eu)
These properties are especially applied to confocal imaging systems, including image scanning microscopy. (edu.au)

Here, a pick-and-place technique for the controlled bottom up assembly of integrated quantum optical devices based on atomic force microscopy combined with optical confocal microscopy is introduced [1]. (dpg-verhandlungen.de)

Although the function of the acousto-optic tunable filter is similar to that of a diffraction grating, its behavior differs in that diffraction occurs over an extended volume of the crystal rather than at a planar surface, and only a limited band of spectral frequencies are affected. (olympusconfocal.com)
My research interests include the theory of electromagnetic problems, diffraction, focusing and microscopy with especial emphasis on confocal microscopy and optical data storage. (imperial.ac.uk)
Une approche de minimisation altern e (AM), restaure les fr quences manquantes au-del de la limite de diffraction, en utilisant une r gularisation par la variation totale sur l objet, et une contrainte de forme sur la PSF. (inria.fr)
Tucson-headquartered scientific camera specialist Photometrics says that thanks to a collaboration with the Netherlands-based microscopy startup Confocal.nl , laboratories are now able to buy a new cost-effective system capable of sub-diffraction-limited resolution. (optics.org)
They add that the signal-to-noise ratio of RCM is four times better than standard confocal microscopy, and confirm that the lateral resolution of the technique is 170 nm, compared with 240 nm for the diffraction-limited approach when using a 488 nm excitation laser and optics with a numerical aperture of 1.49. (optics.org)

The license for two-photon laser microscopy (also known as multiphoton microscopy and protected by patents dating back to 1991) has been transferred from Bio-Rad Laboratories (Hercules, CA) to Carl Zeiss. (laserfocusworld.com)

I was appointed Lecturer in Photonics in 2002 at Imperial College London and in 2006 I was promoted to Reader in Optics and in 2009 to Professor of Optical Physics. (imperial.ac.uk)
Scientists at the University of Strathclyde's Institute of Photonics in Glasgow, UK, have done extensive research into adaptive optics in microscopy, and those results are reported here, along with a glimpse of what might be available commercially in the near future. (photonics.com)
For his fundamental contributions to the field of optics he was awarded the Britton Chance Award from the International Society for Optics and Photonics (SPIE). (msu.edu)
He has made many contributions to the optics and photonics community through Optica/OSA and other societies. (optica.org)
He was general chair (2016-2018) of the Institute of Physics Photon conference, the UK's major optics and photonics conference. (optica.org)
Optica plays a vital role in the optics and photonics community, through its publications, meetings, networks and advocacy for our field. (optica.org)
I believe that my leadership experience in the field of optics and photonics means that I can make valuable contributions across Optica's remit. (optica.org)

Rounding out this series of features is a look at adaptive optics, which was once primarily a tool for astronomers but is now making its way into microscopy, where researchers use it to counter aberrations in their systems. (photonics.com)
in the group of Tony Wilson on the subject of Adaptive Optics for Confocal Microscopy . (optica.org)
He has served on numerous conference committees, including Optica/OSA's Adaptive Optics, Novel Techniques in Microscopy, CLEO Biophotonics, CLEO Europe. (optica.org)
Booth's research centres on the use of adaptive optics to enhance the capabilities of high-resolution optical systems. (optica.org)
Further fundamental advances have been made in spatio-temporal and vectorial adaptive optics. (optica.org)

Dr. Bonin is also an expert in confocal-based microscopy approaches including fluorescence recovery after photobleaching (FRAP) and raster image correlation spectroscopy (RICS). (cancer.gov)

This transparent electrode technology may pave the way for high spatio-temporal resolution electro-optic mapping of the dynamic neuronal activity. (nature.com)

Bio-Rad sold its confocal microscopy business to Carl Zeiss effective June 1. (laserfocusworld.com)

Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. (strath.ac.uk)

This system uses a swept-scan 25-MHz ultrasound transducer with confocal dark-field laser illumination optics. (spie.org)

We determined whether neuroretinal rim assessment based on Bruch's membrane opening (BMO), rather than conventional optic disc margin (DM)-based assessment or retinal nerve fiber layer (RNFL) thickness, yielded higher diagnostic accuracy in myopic patients with glaucoma. (nih.gov)

The acousto-optic tunable filter ( AOTF ) is an electro-optical device that functions as an electronically tunable excitation filter to simultaneously modulate the intensity and wavelength of multiple laser lines from one or more sources. (olympusconfocal.com)
Introduction of the acousto-optic tunable filter to confocal systems overcomes most of the filter wheel disadvantages by enabling rapid simultaneous electronic tuning and intensity control of multiple laser lines from several lasers. (olympusconfocal.com)
During the late 1970s and the 1980s, advances in computer and laser technology, coupled to new algorithms for digital manipulation of images, led to a growing interest in confocal microscopy. (olympusconfocal.com)
1 - Blind Deconvolution for Confocal Laser Scanning Microscopy . (inria.fr)
La microscopie confocale balayage laser, est une technique puissante pour tudier les sp cimens biologiques en trois dimensions (3D) par sectionnement optique. (inria.fr)
Confocal laser scanning microscopy is a powerful technique for studying biological specimens in three dimensions (3D) by optical sectioning. (inria.fr)
Fixed-frozen retinas from adult C57BL/6N mice were used for immunohistochemistry, laser scanning confocal microscopy, and enzymatic histochemistry. (molvis.org)
In this paper, we propose a three-channel fluorescence confocal microscopy system based on fiber bundle and two excitation laser lines of 488nm and 650nm. (jpier.org)
Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. (lu.se)
High resolution is achieved by scanning the sample with a finely focussed laser beam, and exclusion of out-of-focus fluorescence by a confocal aperture. (lu.se)

Signal strength and integrated intensity in confocal and image scannin" by Colin J.R. Sheppard, Marco Castello et al. (edu.au)

Combining pre-selected nanoparticles with nano-or microstructures produced in a top down process is an important but challenging step in the production of hybrid devices for nano-optics. (dpg-verhandlungen.de)

Additional avenues to sources of information on confocal principles, theory, and optics are also provided. (fsu.edu)

He was an associate editor of Optics Express (2007-2013) and Biomedical Optics Express (2010-2013). (optica.org)

The RCM technology will be making further appearances at a number of microscopy events this year, including the ELMI 2017 event in Dubrovnik, Croatia, next month, and the Microscience Microscopy Congress taking place in Manchester, UK, in early July. (optics.org)

The basic concept of confocal microscopy was originally developed by Marvin Minsky in the mid-1950s (patented in 1957) when he was a postdoctoral student at Harvard University. (olympusconfocal.com)

The confocal imager utilizes a Nipkow spinning disk and allows users to quickly switch from confocal to wide-field viewing or recording. (fsu.edu)

Also of note are recent developments in total internal reflection fluorescence microscopy, into which Olympus America provides some insight. (photonics.com)

Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution compared to wide-field microscopy. (wikipedia.org)

There has been an immense drive in modern microscopy towards miniaturization and fibre-based technology. (nature.com)
Here we present a novel approach for this field by utilizing disordered light within a standard multimode optical fibre for lensless microscopy and optical mode conversion. (nature.com)
These may be useful for future fibre-based implementations of super-resolution or light-sheet microscopy. (nature.com)

In contrast, a thin optical section of the same grain (Figure 1(f)) acquired with confocal techniques displays a dramatic difference between the particle core and the surrounding envelope. (olympusconfocal.com)
Dr. Bonin has a broad experience in microscopy including bright and dark-field fluorescence, total internal reflection fluorescence (TIRF), and differential interference contrast (DIC) microscopy. (cancer.gov)

The problem is that the confocal volume sensibly depends on numerous parameters of the optical set-up, refractive index mismatch of the sample solution and the objective's immersion medium, coverslide thickness variations and especially the dependence on optical saturation of the fluorescent dye, which can occur at even very low excitation powers. (picoquant.com)

In fact, confocal technology is proving to be one of the most important advances ever achieved in optical microscopy. (olympusconfocal.com)
As microscopy advances, the demands for enhancements in support technologies increase. (photonics.com)
And as technical advances make their way into commercial products, they fuel further advances in microscopy. (photonics.com)
An example of science driving the technical advances is spinning-disc confocal microscopy. (photonics.com)

The basic key to the confocal approach is the use of spatial filtering techniques to eliminate out-of-focus light or glare in specimens whose thickness exceeds the immediate plane of focus. (olympusconfocal.com)

In another feature, Leica engineers explore recent developments in fluorescence and forensic microscopy. (photonics.com)

Axial resolution as low as 170 nm made possible with pre-aligned optics and mirror-based re-scanning feature. (optics.org)

Prior Scientific, for example, brings automated shutters and focus mechanisms to the lab, allowing ongoing light microscopy studies to be performed as simply and accurately as possible. (photonics.com)
Light microscopy is the most widely used form of microscopy for life science research. (conference-service.com)
We demonstrate that hippocampal slices can be imaged through transparent graphene electrodes by both confocal and two-photon microscopy without causing any light-induced artefacts in the electrical recordings. (nature.com)
And after a first public demonstration of the RCM kit at the European Light Microscopy Initiative (ELMI) conference held in Debrecen, Hungary, in May 2016, the company began delivering the first commercial units earlier this year. (optics.org)

Confocal studies showed differential cellular and compartmental distribution of isozymes involved in glucose, glutamate, glutamine, lactate, and creatine metabolism. (molvis.org)

Ruling out glaucoma in myopic eyes often poses a diagnostic challenge because of atypical optic disc morphology and visual field defects that can mimic glaucoma. (nih.gov)
Myopic subjects with refraction error greater than -2 diopters (D) (spherical equivalent) and typical myopic optic disc morphology, with and without glaucoma, were recruited from a glaucoma clinic and a local optometry practice. (nih.gov)

While the initial research will be conducted in our computational microscopy lab , its application is to study developmental biology in collaboration with our collaborators at the School of Life Sciences . (jobs.ac.uk)

His original training is in physics, specifically optics, and in the last 15 years has developed biophysics projects. (cancer.gov)
He has over 60 publications in physics, optics, and biophysics journals. (cancer.gov)

Little is known about the structure of RGC axon bundles extending from individual RGC somas to the optic nerve head (ONH) and how they respond to disease insults. (jneurosci.org)
Nonspecific optic nerve head swelling (papillitis), direct invasion of the optic disc or nerve, or papilledema due to increased intracranial pressure may be present. (medscape.com)
Examination of the optic nerve head may reveal edema, vascular engorgement, nerve head elevation, hemorrhage, or optic nerve pallor or frank atrophy. (medscape.com)

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