• compounds
  • Makoto Koizumi in 2004 reviewed the properties of BNAs with focus on ENAs as antisense and antigen oligonucleotides (AONs) and proposed an action mechanism for these compounds that may involve translation arrest, mRNA degradation mediated by RNase H and splicing arrest. (wikipedia.org)
  • polymerase
  • Oligonucleotides are provided which hybridize to the viral RNA segments of influenza viruses or to certain mRNA's which encode the NP, M1, M2, NS1, NS2 or other key proteins of influenza viruses, including RNA polymerase, hemagglutinin, nucleoprotein or neuraminidase. (google.com)
  • Oligonucleotides composed of 2'-deoxyribonucleotides (oligodeoxyribonucleotides) are fragments of DNA and are often used in the polymerase chain reaction, a procedure that can greatly amplify almost any small amount of DNA. (wikipedia.org)
  • nuclease
  • During anti-miRNA oligonucleotide design, necessary modifications to optimize binding affinity, improve nuclease resistance, and in vivo delivery must be considered. (wikipedia.org)
  • backbone
  • The bridged ribose conformation enhances base stacking and pre-organizes the backbone of the oligonucleotide significantly increasing their hybridization properties. (wikipedia.org)
  • proteins
  • Rather than modulating the activity of already-formed proteins, antisense oligonucleotides act before proteins are produced at the level of messenger RNA in the cell, thus opening up new opportunities for therapeutic intervention. (in-pharmatechnologist.com)
  • Understanding the miRNA sequences involved in these diseases can allow us to use anti miRNA Oligonucleotides to disrupt pathways that lead to the under/over expression of proteins of cells that can cause symptoms for these diseases. (wikipedia.org)
  • Alignment
  • SOAP (Short Oligonucleotide Analysis Package) is a suite of bioinformatics software tools enabling the assembly, alignment, and analysis of next generation DNA sequencing data. (wikipedia.org)
  • interactions
  • In particular, this invention relates to antisense oligonucleotide interactions with certain viral ribonucleic acids and messenger ribonucleic acids involved in the infection of cells by influenza viruses. (google.com)
  • The ability to use oligonucleotide interactions to control lipid particle stability represents a new approach in the design of programmed nanoscale devices. (nih.gov)
  • chemical
  • Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not have this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. (wikipedia.org)
  • genetic
  • Following this methodology, sets of tri- and tetradeoxyribonucleotides were synthesized and were enzymatically converted to longer oligonucleotides, which allowed elucidation of the genetic code. (wikipedia.org)
  • diseases
  • The collaboration is looking to develop and commercialise several antisense oligonucleotides to treat genetically-defined neurological diseases, including Huntington's disease, amyotrophic lateral sclerosis (ALS) - commonly referred to as Lou Gehrig's disease -frontotemporal dementia (FTD) and spinocerebellar ataxia type 3 (SCA3). (in-pharmatechnologist.com)
  • Antisense oligonucleotides have been researched as potential drugs for diseases such as cancers (including lung cancer, colorectal carcinoma, pancreatic carcinoma, malignant glioma and malignant melanoma), diabetes, amyotrophic lateral sclerosis (ALS), Duchenne muscular dystrophy, spinal muscular atrophy, Ataxia-telangiectasia and diseases such as asthma, arthritis and pouchitis with an inflammatory component. (wikipedia.org)
  • gene
  • Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. (springer.com)
  • Wave's lead oligonucleotide candidates are WVE-120101 and WVE-120102, which selectively target the mutant allele of the huntingtin (HTT) gene. (in-pharmatechnologist.com)
  • If binding takes place this DNA/RNA hybrid can be degraded by the enzyme RNase H. The use of morpholino-antisense oligonucleotides for gene knockdowns in vertebrates, which is now a standard technique in developmental biology and is used to study altered gene expression and gene function, was first developed by Janet Heasman using Xenopus. (wikipedia.org)
  • short
  • We have engineered serum stabilized micelles using short quadruplex forming oligonucleotide extensions as the lipid headgroup. (nih.gov)
  • therapeutic
  • The findings supported the hypothesis that PCSK9 is a potential therapeutic target for hypercholesterolemia and the researchers were able to show that BNA-based antisense oligonucleotides (AONs) induced cholesterol-lowering action in hypercholesterolemic mice. (wikipedia.org)
  • method
  • The most common experimental method for determining T m is to measure the absorbance change of the oligonucleotide with its complement as a function of temperature ( Figure 1 ). (sigmaaldrich.com)
  • The nearest neighbors method accounts for thermodynamic as well as other factors that affect T m , including oligonucleotide and monovalent cation concentrations. (sigmaaldrich.com)