• compounds
  • Makoto Koizumi in 2004 reviewed the properties of BNAs with focus on ENAs as antisense and antigen oligonucleotides (AONs) and proposed an action mechanism for these compounds that may involve translation arrest, mRNA degradation mediated by RNase H and splicing arrest. (wikipedia.org)
  • polymerase
  • Oligonucleotides are provided which hybridize to the viral RNA segments of influenza viruses or to certain mRNA's which encode the NP, M1, M2, NS1, NS2 or other key proteins of influenza viruses, including RNA polymerase, hemagglutinin, nucleoprotein or neuraminidase. (google.com)
  • Oligonucleotides composed of 2'-deoxyribonucleotides (oligodeoxyribonucleotides) are fragments of DNA and are often used in the polymerase chain reaction, a procedure that can greatly amplify almost any small amount of DNA. (wikipedia.org)
  • backbone
  • The bridged ribose conformation enhances base stacking and pre-organizes the backbone of the oligonucleotide significantly increasing their hybridization properties. (wikipedia.org)
  • gene
  • The main obstacles in using antisense oligonucleotides to inhibit gene expression are cellular instability, low cellular uptake, and poor intracellular delivery. (google.com.au)
  • If binding takes place this DNA/RNA hybrid can be degraded by the enzyme RNase H. The use of morpholino-antisense oligonucleotides for gene knockdowns in vertebrates, which is now a standard technique in developmental biology and is used to study altered gene expression and gene function, was first developed by Janet Heasman using Xenopus. (wikipedia.org)
  • Alignment
  • SOAP (Short Oligonucleotide Analysis Package) is a suite of bioinformatics software tools enabling the assembly, alignment, and analysis of next generation DNA sequencing data. (wikipedia.org)
  • chemical
  • Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not have this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. (wikipedia.org)
  • diseases
  • Understanding the miRNA sequences involved in these diseases can allow us to use anti miRNA Oligonucleotides to disrupt pathways that lead to the under/over expression of proteins of cells that can cause symptoms for these diseases. (wikipedia.org)
  • Antisense oligonucleotides have been researched as potential drugs for diseases such as cancers (including lung cancer, colorectal carcinoma, pancreatic carcinoma, malignant glioma and malignant melanoma), diabetes, amyotrophic lateral sclerosis (ALS), Duchenne muscular dystrophy, spinal muscular atrophy, Ataxia-telangiectasia and diseases such as asthma, arthritis and pouchitis with an inflammatory component. (wikipedia.org)
  • composition
  • 1. A composition comprising a liposome comprising a) a substantially uncharged lipid material comprising one or more phospholipids, and b) a p-ethoxy oligonucleotide that is entrapped in the lipid material. (google.com.au)
  • 5. The composition of claim 1 , where the molar ratio of phospholipid to p-ethoxy oligonucleotide is less than about 3,000:1. (google.com.au)
  • method
  • The most common experimental method for determining T m is to measure the absorbance change of the oligonucleotide with its complement as a function of temperature ( Figure 1 ). (sigmaaldrich.com)
  • specifically
  • In accordance with preferred embodiments, oligonucleotides and oligonucleotide analogs are provided which are specifically hybridizable with viral RNAs. (google.com)
  • In other preferred embodiments, the oligonucleotides are specifically hybridizable with a transcription initiation site, a translation initiation site, 5'-untranslated sequences, 3'-untranslated sequences, and intron/exon junction of influenza virus mRNAs. (google.com)
  • In additional preferred embodiments, the oligonucleotides are specifically hybridizable with RNA sequences involved in splicing of the viral RNA, or in viral packaging. (google.com)