• Genes
  • Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression. (cancerindex.org)
  • Therefore, we used BRB (Biometrics Research Branch) class comparison tool with multivariate analysis to identify differentially expressed genes in human cutaneous melanomas, compared with benign nevi and normal skin derived from the microarray dataset (GDS1375). (cancerindex.org)
  • We used their genotypes at 5,941,815 nucleotide variants obtained by Genome Analyzer II and SOLiD.The association analysis revealed 4166 nucleotide variants associated with expression of 85 genes (P (cancerindex.org)
  • This provides a relatively low-cost microarray that may be customized for each study, and avoids the costs of purchasing often more expensive commercial arrays that may represent vast numbers of genes that are not of interest to the investigator. (wikipedia.org)
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • Analysis of custom genomic regions of interest (e.g. specific genes, multiple variants and/or complete pathways). (wikipedia.org)
  • Synthetic biology) An example of a recent application is testing of the BRCA1 and BRCA2 breast cancer risk genes The FlexArrayer is an in-house custom oligonucleotide synthesis instrument. (wikipedia.org)
  • In 2011, the genome of an exceptional bladder cancer patient whose tumor had been eliminated by the drug everolimus was sequenced, revealing mutations in two genes, TSC1 and NF2. (wikipedia.org)
  • A computational screen based on promoter sequences recognised by the sigma factor sigma 70 and on Rho-independent terminators predicted 24 putative sRNA genes, 14 of these were verified experimentally by northern blotting. (wikipedia.org)
  • From this set of candidate genes, microarray analysis and northern blotting confirmed the existence of 17 previously undescribed sRNAs, many of which bind to the chaperone protein Hfq and regulate the translation of RpoS (Sigma 38). (wikipedia.org)
  • Esr41 sequence is not present in nonpathogenic E. coli K12, but the sRNA can induce cell motility in K12 as well, suggesting that target genes controlled by Esr41 are present in both E.coli. (wikipedia.org)
  • PROBES
  • Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). (wikipedia.org)
  • Many types of arrays exist and the broadest distinction is whether they are spatially arranged on a surface or on coded beads: The traditional solid-phase array is a collection of orderly microscopic "spots", called features, each with thousands of identical and specific probes attached to a solid surface, such as glass, plastic or silicon biochip (commonly known as a genome chip, DNA chip or gene array). (wikipedia.org)
  • Arrays from commercial vendors may have as few as 10 probes or as many as 5 million or more micrometre-scale probes. (wikipedia.org)
  • The probes are synthesized prior to deposition on the array surface and are then "spotted" onto glass. (wikipedia.org)
  • A common approach utilizes an array of fine pins or needles controlled by a robotic arm that is dipped into wells containing DNA probes and then depositing each probe at designated locations on the array surface. (wikipedia.org)
  • These arrays may be easily customized for each experiment, because researchers can choose the probes and printing locations on the arrays, synthesize the probes in their own lab (or collaborating facility), and spot the arrays. (wikipedia.org)
  • Current cancer research makes use primarily of DNA microarrays in which an arrayed series of microscopic spots of pre-defined DNA oligonucleotides known as probes are covalently attached to a solid surface such as glass, forming what is known as a gene chip. (wikipedia.org)
  • After amplifying one DNA oligoprobe of interest, it can be used to create 100 different probes on 100 different sets of microspheres, each with the capability of capturing 100 potential targets (if using a 100-plex array). (wikipedia.org)
  • microarrays
  • Play media Microarrays can be fabricated using a variety of technologies, including printing with fine-pointed pins onto glass slides, photolithography using pre-made masks, photolithography using dynamic micromirror devices, ink-jet printing, or electrochemistry on microelectrode arrays. (wikipedia.org)
  • DNA microarrays evolved from Southern blotting which allows for detection of a specific DNA sequence in a sample of DNA. (wikipedia.org)
  • High array density/multiplex: Compared to flat microarrays, SAT allows one to perform parallel measurements. (wikipedia.org)
  • siRNA
  • The program is a comprehensive real time PCR primer and probe search and analysis tool, and also does other tasks such as siRNA and molecular beacon searches, open reading frame and restriction enzyme analysis etc. (wikipedia.org)
  • metabolism
  • used Ingenuity Pathway Analysis (IPA) software that associated exRNAs found in human blood microvesicles with pathways involved in blood cell differentiation, metabolism, and immune function. (wikipedia.org)
  • microscopic
  • The alternative bead array is a collection of microscopic polystyrene beads, each with a specific probe and a ratio of two or more dyes, which do not interfere with the fluorescent dyes used on the target sequence. (wikipedia.org)
  • quantitative
  • Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. (cancerindex.org)
  • protein
  • The next year protein sequencing and amino acid samples were sent to survey 103 core facilities. (wikipedia.org)
  • Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. (wikipedia.org)
  • Tumor
  • To investigate the function of TGF-β1 and test whether fascin1 is an important mediator of the tumor response to TGF-β1 in bladder carcinoma cells, real-time RT-PCR and western blot analysis were used to test changes in fascin1 expression after TGF-β1 (10 ng/ml) treatment in T24 and BIU87 cells. (cancerindex.org)
  • Access to whole cancer genome sequencing is important to cancer (or cancer genome) research because: Mutations are the immediate cause of cancer and define the tumor phenotype. (wikipedia.org)
  • Cell Cycle
  • Through exogenous expression analyses, we found that PDCD2 and NCoR1 can decrease proliferation, and increase apoptosis and G1 cell cycle arrest, in GIST-derived cells. (cancerindex.org)
  • Chemical
  • Deep sequencing of RNA expressed during chemical stress and high cell density fermentation discovered 253 novel intergenic transcripts adding to roughly 200 intergenic sRNAs previously described in E. coli. (wikipedia.org)
  • Transduction
  • The actual sensing component (or "chip") is just one piece of a complete analysis system Transduction must be done to translate the actual sensing event (DNA binding, oxidation/reduction, etc.) into a format understandable by a computer (voltage, light intensity, mass, etc.), which then enables additional analysis and processing to produce a final, human-readable output. (wikipedia.org)