Often range biological assay. Medical search. Frequent questions

There's a lot of political talk that the tiny and decreasing number of women in computing is a result of sexism and prejudice that has to be remedied with measures ranging from sensitivity training up through admission and hiring quotas. (ibiblio.org)
In summary, 1) the assay standard curves and the relative signals of the calibrator concentrations were consistently within the criteria specified by the manufacturer, 2) the obtained assay sensitivity was calculated to be 4.33 pg/mL while the manufacturer claimed sensitivity was 6.15 pg/mL, 3) two samples with very high and high Klotho concentrations were used at different dilution to evaluate the assay linearity. (cdc.gov)
The sensitivity and dynamic range of the QIAseq Library Quant System were compared against Agilent's High Sensitivity DNA Kit, using two NGS libraries of differing concentrations. (qiagen.com)
By contrast, the QIAseq Library Quant Array was able to quantify both libraries (see figure The QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods The QIAseq Library Quant Array's high sensitivity and broad dynamic range enable the quantification of both the NGS-L1 and NGS-L2 libraries [A]. By contrast, Agilent's High Sensitivity DNA Kit (for use with the Agilent 2100 BioAnalyzer) quantified only the NGS-L1 library; with this kit, the NGS-L2 concentration was too low for quantification [B]. "> QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods ). (qiagen.com)
The QIAseq Library Quant System is available in both a tube and an array format, and is optimized with QIAseq qPCR SYBR ® Green Mastermixes to provide superior sensitivity and a wide dynamic range. (qiagen.com)
This includes studies on the effects of low doses, group related radiation sensitivity, and epidemiological studies where sampling and handling of biological samples is included. (europa.eu)
Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)
Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
ENVI Assay System test kits are featured with practical tools and straightforward test method giving fast response, specificity, and sensitivity ideal for provisional biological threat assessment from environmental samples. (j3seven.com)

ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)
These antibodies can be utilized over an extensive application range including Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA, ELISpot and Biological assays. (diaclone.com)
Antinuclear antithrombin, protein C, protein S or pres- antibodies were investigated with standard- ence of antiphospholipid antibodies, are ized enzyme-linked immunosorbent assay common in patients with retinal vein occlu- sions and may contribute to the etiology of (ELISA). (who.int)

Can be readily automated as a high-throughput assay for thousands of samples per day. (immpact-international.org)

Develop, optimize, establish and conduct a wide range of biological, molecular and biochemical assays. (nature.com)
Techniques used span from synthetic chemistry to protein expression and biochemical assays. (hofstra.edu)
The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)
Hence, it is desirable to start with a biochemical assay followed by a cell-based assay to corroborate results in a physiological context (that is, to provide orthogonal validation). (genengnews.com)

SAGIAN Automated Assay Optimization (AAO) software is now allowing scientists to import designed experiments that are translated into corresponding Biomek FX methods. (qualitydigest.com)
To overcome these obstacles, automated assay optimization (AAO) systems often are used to accommodate large numbers of samples. (statease.com)
Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)

We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. (nih.gov)
Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)

In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
DCM is very volatile and thus sampling and storage conditions of biological fluids are important. (who.int)

By combining allele-specific amplification and hydrolysis probe detection, qBiomarker Somatic Mutation real-time PCR assays have been developed which can detect as few as 1% somatic mutations in the background of wild-type genomic DNA. (qiagen.com)
For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044 . (abcam.com)

These studies use a range of techniques, from computational modelling, to biological assays, to Nuclear Magnetic Resonance Spectroscopy (NMR). (hofstra.edu)
Simulations can now be used as computational assays of biological activity, for example, in predictions of drug resistance. (manchester.ac.uk)
Methodological and algorithmic developments, combined with advances in computational hardware, are transforming the scope and range of calculations. (manchester.ac.uk)

Diaclone Monoclonal Antibodies are highly specific, high quality mouse monoclonal antibodies for the detection of a broad range of human proteins. (diaclone.com)

Studies are screened, and only those assays using standardized methods and resulting in significant effects are used for the determination of ERL/ERM guidelines. (wikipedia.org)
Most of the assay methods for quantifying uromodulin rely on the availability of antibodies to this glycoprotein. (tubitak.gov.tr)
These methods may also incorporate simple calculated properties, for example the so-called 'Rule of 5' (5) to ensure libraries stay within sufficiently druglike areas of chemical space as well as existing structure activity relationship data describing the interaction of the chemical matter with the principle biological target, as well as related targets. (ddw-online.com)
Spectrophotometric methods were used to assay the clotting factors II, VII, IX and X in plasma of 33 subjectively healthy human donors and in plasma of 98 patients receiving long-term oral anticoagulant therapy. (karger.com)
Plasma from 98 patients receiving long-term anticoagulant therapy was assayed with 'Thrombotest', a clotting test in whole blood introduced by Owren and in these plasmas the activity of each of the vitamin K-dependent factors was assayed with spectrophotometric methods. (karger.com)
For the clotting factors IX and VII, novel spectrophotometric methods were applied and the plasma activities thus measured were compared to results obtained with factor IX and VII clotting assays. (karger.com)
This assay is less prone to artifacts than other viability assay methods. (sigmaaldrich.com)
The objective of this article is to inform about efforts to design and implement a data model that can parametrically describe and store information about a wide range of ecotoxicological tests and bioindication methods used in Ecological Risk Assessment (EcoRA). (muni.cz)
The aim of this work is to complement existing EcoRA decision-support tools with a web-based engine for storing and searching biological tests and methods used in EcoRA as there is currently no similar informational source available on the Internet. (muni.cz)

Additionally, for complex biological matrices, antibody selection can be challenging since protein targets secreted from cells into cell culture media or serum may be cleaved or modified, altering epitope recognition-hence the benefit of using protease cocktail inhibitors. (genengnews.com)
As each study had used different assays for measuring testosterone, with each assay using different calibrators, the team obtained fasting morning serum samples from 100 men in each cohort, which were then analyzed in the CDC Clinical Reference Laboratory using a single, highly calibrated assay. (medscape.com)

Microfluidic devices are essential in performing scientific tests ranging from DNA sequencing to cell sorting and protein analysis. (enparticle.com)
However, although Michael acceptors are reactive, the Michael addition is reversible and it can be assumed that also other parts of the molecules will moderate the binding to and the release from any given nucleophilic site in a protein, and thereby moderate a specific biological activity. (lu.se)

In environmental toxicology, effects range low (ERL) and effects range median (ERM) are measures of toxicity in marine sediment. (wikipedia.org)
They are derived from biological toxicity assays and synoptic sampling. (wikipedia.org)
NOAA originally calculated ERL/ERMs using existing toxicity data compiled from completed toxicity assays with varying endpoints, including effects on commonly tested organisms, particularly at sensitive life stages. (wikipedia.org)
Using data already collected ("data mining") has the advantage of being able to quickly and inexpensively make an assessment with a large dataset that would otherwise require much more time-consuming and costly specific toxicity assays. (wikipedia.org)
In summary, the key links between the compiled studies are the testing of a specific analyte - toxicity assays used are for sediment, and a significant effect must be determined. (wikipedia.org)

While highlighting that the findings establish a reference range for testosterone, he added: "These data also show that variations in assays are an important contributor to variation in testosterone levels in cohorts from different geographic regions. (medscape.com)
Inter-assay and intra- about 4.4 million people, had impaired disorders such as hypo- or hyperthy- assay variations for PTH were 8.9% and fasting glucose [2,3]. (who.int)

Performance parameters that may need to be established during validation include accuracy, precision, specificity, limit of detection ( LOD ), limit of quantification ( LOQ ), linearity, and range. (gmp-compliance.org)

Potency is determined by means of a biological assay and interpreted by the first International Low Molecular Weight Heparin Standard as units of anti-factor Xa (anti-Xa) activity per milligram. (rxlist.com)
Effective use of hierarchical lines of evidence relies on consideration of the rele- vance and potency of assays, organization of assays by mode of action, and better assay validation. (cdc.gov)

The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Synthesis, in vitro transcription inhibition assays, and biological testing of the hybrids identified a range of potent anti-transcription inhibitors with activity against a range of pathogenic bacteria with MICs as low as 3.1 μM. (edu.au)

Assay technologies relying on excitation or emission at these wavelengths will be prone to interference. (genengnews.com)

If it is outside the range considered acceptable for the test (i.e., 28 to 32 g/kg), the salinity of the sample or each test solution should be adjusted to within this range using natural or artificial hypersaline brine, commercially-available dry ocean salts, reagent-grade salts (see Sections 2.3.4 and 4.3.2 ), or deionized water. (canada.ca)

This study investigates a range of solitary and social species across Aculeata. (bvsalud.org)

Low interference in biological samples. (immpact-international.org)

In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
The assay is often repeated multiple times on certain molecules. (physicsforums.com)
Biomolecular simulation is increasingly central to understanding and designing biological molecules and their interactions. (manchester.ac.uk)
Wash-based assays can be used to circumvent interference caused by highly complex sample types, as molecules within the matrix that contribute to interference are washed away. (genengnews.com)

To evaluate the use of salivary ChE as a biological monitor for OP insecticide exposure, a modified Ellman assay in conjunction with a pharmacodynamic model was used to characterize salivary ChE in adult male Sprague-Dawley rats. (cdc.gov)

Assay technologies may not always be compatible with particular matrices, which can cause interference. (genengnews.com)

The ATP Bioluminescence Kit provides a simple and quick assay to quantify ATP, and also to measure cell viability based on ATP content. (sigmaaldrich.com)
This lactone ring is a Michael acceptor that is known to affect biological processes such as cell proliferation, death/apoptosis, and cell migration, by interfering with nucleophilic sites in cell signalling pathways. (lu.se)
The IC50 values in the two cell lines were found to depend on the overall structure of the assayed compounds, although less in this subset of compounds compared to a previous investigation. (lu.se)

Find or custom design the right target-specific assays and panels to research your biological targets of interest. (qiagen.com)
Offer assays formulated for mixing into panels of up to 30-plex and are all sold individually to provide a highly flexible system. (bdbiosciences.com)

The emission spectra is a scan of the emission range, normally from the wavelength of excitation in the direction of the longer wavelengths resulting from excitation at the excitation max of the fluorophore. (biolegend.com)
Since the Aurora utilizes the full spectrophotometric profile of the fluorophores across all lasers to accurately unmix the fluorophores, the spectra of the Aurora is best seen as only the emission profiles collected in segments of 10-30nm across the emission range (longer than the excitation laser) off each laser. (biolegend.com)

All assays within each product area have been verified for performance in single-plex and in multiplexed scenarios to ensure consistency between assay types. (bdbiosciences.com)

In the research described in the paper for example, we have been able to not only synthesise a range of novel quinoxaline-alkynyl derivatives but then also carry out in-vitro testing to investigate the biological activity of these compounds. (asynt.com)
As a follow-up of a previous investigation in which semisynthetic damsin derivatives were shown to possess up to 10 times higher cytoxicity in JIMT-1 breast cancer cells compared to normal breast epithelial MCF-10A cells, a range of new derivatives were prepared and assayed toward the same cells. (lu.se)

When the therapeutic range for Thrombotest values is set between 5 and 12.5% the corresponding therapeutic ranges for the activity of the factors II, VII, IX and X are respectively 12.6-36.1, 27.0-52.3, 23.1-49.3 and 18.9-36.2% (expressed as a percentage of the activity in normal pool plasma). (karger.com)

The requirement is to develop high-quality, robust assays that work across a wide range of biological conditions. (qualitydigest.com)
Integrating DOE and automated, liquid-handling technology offers scientists the ability to design experiments that test a wide range of assay conditions. (qualitydigest.com)
Develop the skills and knowledge to make a difference in the world with the hands-on experience you need to thrive in a wide range of bioscience fields. (mdx.ac.uk)
The primary mechanism of action for organophosphorus (OP) insecticides such as chlorpyrifos (CPF) involves the inhibition of acetylcholinesterase (AChE) by their active oxon metabolites resulting in a wide range of neurotoxic effects. (cdc.gov)
And, our prestigious journals publish the latest scientific findings on a wide range of topics. (nationalacademies.org)
In addition, there was a wide range of adaptive ability levels in children with autism in our study, including in those with low, average, or high cognitive scores. (cdc.gov)

The measuring range of the bioassay described in this paper is between 0.13 and 1.25 \mu g/ml, which makes it a potential method for clinical use. (tubitak.gov.tr)
Well-defined reference ranges are at the heart of clinical practice and, without them, clinicians can make erroneous diagnoses that could lead to patients receiving costly, lifelong treatments that they don't need or deny treatments to those who need them," said Dr Bhasin said in an Endocrine Society press release. (medscape.com)

The chromogenic assays for the factors II, VII, IX and X provide the same information on the therapeutic state of the patients in respectively 86.7, 78.6, 81.6 and 89.8% of the cases. (karger.com)
Collectively, these factors are known to influence data quality, biological relevance, and therapeutic predictability, which will ultimately impact the success of entire preclinical drug development efforts. (genengnews.com)

Age and assay variation values for 25(OH)D from the third national Surveil ance of sex were matched between the 2 groups. (who.int)

qBiomarker Somatic Mutation PCR Arrays are translational research tools that allow rapid and accurate profiling of the somatic mutation status for important genes related to a biological pathway or disease. (qiagen.com)
Our research programs are diverse and include a range of diseases and research topics such as cancer, diabetes, ocular diseases, as well as novel formulation delivery platforms, drug discovery/developments and drug metabolism. (samford.edu)
Our laboratory facilities occupy over 1,000 square feet of space and consist of modern chromatographic, spectrometric and instruments for biological based assays which are available to students conducting research. (samford.edu)
A European network of biological and physical-retrospective dosimetry "RENEB" has been set up which has the capacity and capability to significantly contribute to European Radiation Emergency Preparedness and to Radiation Protection Research in Europe. (europa.eu)
The commit tee also believes that since the term elongate mineral particle covers a broad range of mineral particles of interest in this research, its use should be limited to research efforts and emphasizes that this term is not a rigorous mineralogical term. (nationalacademies.org)

Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
The Toolkit was revised to allow researchers to select self-or interviewer administration when creating the data collection worksheets and ranges of specimens necessary to run biological assays has been added to the Toolkit. (rti.org)
Consequently, small-molecule drug discovery usually involves an iterative process of molecular design, chemical synthesis, biological assay and data analysis feeding directly into the next cycle, but this process always needs a chemical starting point. (ddw-online.com)
The array format workflow (see figure QIAseq Library Quant Array workflow Each sample DNA library is combined with QIAseq qPCR SYBR ® Green Mastermix, aliquoted in technical triplicate across the QIAseq Library Quant Array, and analyzed with qPCR. Raw C T values are copied into the data analysis Excel template, and the software automatically calculates the library concentration or dilution factor. "> QIAseq Library Quant Array workflow ) begins with two tenfold dilutions of the sample library, to ensure that its concentration falls within the range of the serially diluted standards. (qiagen.com)

The oxidation peak current was found to vary linearly with the concentration of nevirapine in the range of 5.0 - 350 µM. (bgu.ac.il)

Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
The array format provides this standard in five predispensed, sequential 10-fold dilutions mixed with a PCR primer assay in triplicate, ensuring that your sample library will fall within the detection range of the array (see figure Principle of the QIAseq Library Quant System The serial dilutions of the DNA standard (5 sequential 10-fold dilutions) generate a standard curve. The sample library should fall within the standard curve. "> Principle of QIAseq Library Quant System ). (qiagen.com)

In some cases (e.g., biological assays), relative accuracy takes the place of accuracy. (gmp-compliance.org)

Procedure: Assay takes 30 min. (immpact-international.org)

Control, reference, and treatment test mixtures were incubated at temperatures ranging from 21.6 to 22.2 °C and aerated for three hours at a rate sufficient to provide aerobic conditions and maintain solids in suspension. (europa.eu)
ENVI Assay System biodefence tests provide military and civil defence officers with a fast, simple, and reliable solution. (j3seven.com)

Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. (abcam.com)

Harmonized reference normal ranges for testosterone levels in healthy, nonobese men have been generated in a landmark study that should aid in accurately diagnosing hypogonadism , say US investigators. (medscape.com)

Hi everyone, I have a question about the error on certain biological results. (physicsforums.com)
Although I no longer spend my days in a lab, as a medicinal chemist myself, I always found it highly motivating to almost see the fruits of your labour - synthesising novel compounds and then actually seeing them have an effect against a certain biological target is hugely rewarding. (asynt.com)

These rapid biodefence tests can be used in a broad range of applications, from law enforcement authorities, to private security services, airport security checks, to even mobile laboratories. (j3seven.com)
Coauthor Hubert Vesper, PhD, who is cochair of the Partnership for the Accurate Testing of Hormones (PATH), an Endocrine Society initiative, commented, "Without harmonized reference ranges and standardized assays, tests can lead to misdiagnoses, and unfortunately this happens every day around the world. (medscape.com)
He emphasized that it is important that the new reference range for testosterone be taken "into consideration in the tests that clinicians and patients depend on for accurate diagnoses. (medscape.com)
According to one proposition, the assays would indicate reference range thyroid hormone levels in the blood if appropriate tests were applied. (medscape.com)

This allows for a clear understanding of the assay and its components so that improvements can be implemented and the assay optimized. (qualitydigest.com)

After ranking, both the 10th and 50th percentile concentrations are determined over the range of endpoint concentrations. (wikipedia.org)
The USGS has also integrated ERL and ERM values into an Alert Range Table, a table that provides ranges of contaminant concentrations that predict the likelihood of adverse effects occurring in benthic organisms for the Lake Pontchartrain Basin in Louisiana. (wikipedia.org)

The biological test system was a consortium of microorganisms common to the activated sludge treatment process. (europa.eu)

More important, measuring how the change of one single factor affects the assay leaves the experimenter blind to interactions that may exist between two or more experimental factors. (qualitydigest.com)
These experimental parameters represent only a few of the many influencing assay factors that are currently being studied using DOE. (qualitydigest.com)

Lampire Biological Laboratories, Inc. (lampire.com)
National laboratories used WHO reference assays developed by the United States Centers for Disease Control and Prevention, an RSV subtyping assay developed by the Victorian Infectious Diseases Reference Laboratory (Australia), or other in-house or commercial assays already in use at their laboratories. (cdc.gov)

Harmonizing testosterone levels from four major cohort studies, Shalender Bhasin, MD, from Brigham and Women's Hospital, Harvard Medical School in Boston, Massachusetts, and colleagues were able to identify a reference range for testosterone that could help clinicians in decision making and improve patient care - this was in the region of 250 to 900 ng/dL for 19- to 39-year-old men. (medscape.com)
As testosterone levels decline with age, the researchers initially generated reference ranges in healthy, nonobese men aged 19 to 39 years, followed by ranges stratified by decade of age. (medscape.com)
In the sickest patients who manifest low T4, TSH elevates to hypothyroid levels at the recovery phase, returning to reference range levels with complete recovery, as shown in the image below. (medscape.com)

DCM assays were sensitive enough to separate workers exposed to 10 ppm from non-exposed workers. (who.int)

Optimizing biological assay conditions is a demanding process that scientists face every day. (qualitydigest.com)
Using mathematical models, they're evaluating experimental parameters in order to optimize assay conditions. (qualitydigest.com)

Collaborations with other departments are of paramount importance since chemists are not able to perform most of the biological assays required to determine the efficacy of the synthesised compounds. (asynt.com)

Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. (abcam.com)
At the same time it describes comprehensive web-based portal built on this model that can be used to quickly find relevant biological assays (ecotoxicological biotests) for given situation and therefore support the decision-making process in EcoRA. (muni.cz)
However, such assays sometimes require complex execution, which can impede the screening process. (genengnews.com)
ENVI Assay System allows for early detection and BWA identification, which combined with timely warning, protection and correct medical countermeasures are crucial steps for the whole process of bio-threat management. (j3seven.com)

Anatomy3

Organisms2

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes12

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Sensitivity10

ELISA4

High-throughput assay1

Biochemical5

Optimization3

Phenotypic2

Fluids2

Detect2

Computational3

Proteins1

Methods9

Serum2

Protein2

Toxicity5

Variations2

Linearity1

Potency2

Extracts1

Inhibitors1

Prone1

Brine1

Species1

Samples1

Molecules4

Evaluate1

Interference1

Cell3

Panels2

Excitation2

Consistency1

Derivatives2

Plasma1

Wide6

Clinical2

Therapeutic2

Variation1

Research5

Data4

Concentration1

Detection range2

Accuracy1

Procedure1

Provide2

Apoptosis1

Accurately1

Certain biological2

Tests4

Components1

Concentrations2

Test1

Factors2

Laboratories2

Reference3

Sensitive1

Conditions2

Compounds1

Process4