• Expression of Rb was detected in normal lung tissues but was barely detectable by Northern blot hybridization in lung tumors. (jax.org)
  • Expression of genes was studied using Northern blot analysis and in situ hybridization. (gla.ac.uk)
  • Second, I conducted extensive analysis of expression profiling of the \u003ci\u003eGsdmc\u003c/i\u003eand \u003ci\u003eGsdmd\u003c/i\u003egenes by Northern blot, quantitative PCR and \u003ci\u003ein situ\u003c/i\u003e hybridization. (soken.ac.jp)
  • The results of these initial studies suggest the possibility of a functional role for GIP in the rat salivary gland by the demonstration of GIP mRNA in the SSG by Northern analysis and in situ hybridization, as well as by an increase in SSG GIP gene expression following a glucose meal. (duke.edu)
  • Cells were treated for 3 days with nontoxic concentrations of the drugs, and CYP3A induction was assessed through midazolam hydroxylase activity and Western and Northern blot analyses. (aspetjournals.org)
  • This result was in agreement with the high amount of CYP3A4 protein and mRNA revealed by Western and Northern blot analyses. (aspetjournals.org)
  • Northern blot analyses indicated that the mRNAs of p50 and p52, but not that of p65 RelA, are induced by TNF or either of the two TNFR antisera. (jefferson.edu)
  • We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)
  • In chemically induced mouse lung tumors, the p16INK4a gene is a target of inactivation, and Rb is reduced at the mRNA level (Northern blot) although similar at the protein level (Western blot) when compared to normal lung tissues. (jax.org)
  • Western blot analysis indicated the presence of both hypophosphorylated and hyperphosphorylated Rb protein in lung tumors and in normal lung tissues. (jax.org)
  • Chloroplasts were purified by Percoll gradient, and the coat protein and virus-associated RNAs were extracted and analyzed by Western and Northern blots respectively. (unesp.br)
  • The effects of PTX-induced exosomal circBACH1 on BC cell function were assessed using RNA Binding Protein Immunoprecipitation (RIP), dual luciferase reporter gene, tube formation, CCK-8, and Western Blot assays. (biomedcentral.com)
  • NHE protein and mRNA were assessed by Western and Northern blotting. (elsevierpure.com)
  • In rats, region-specific, time- and dose-dependent reductions of NHE2 and NHE3 activities, protein expression, and mRNA were observed after exposure to IFN-γ. (elsevierpure.com)
  • Western blotting was used to confirm asymptomatic VL ( 8 ). (cdc.gov)
  • Western blot analysis confirmed the increased expression of cyclin D1 and cdk4 in lung tumors compared to normal lung. (jax.org)
  • Cloroplastos foram purificados em gradiente de Percoll e as proteínas e os RNA foram extraídos e analisados, respectivamente, por Western blot e Northern blot. (unesp.br)
  • Cysticercosis serum apDia antigen ELISAs and Western blot for LLGP and rT24h antigen were performed on all PWE and 2% of screen-negative patients. (cdc.gov)
  • Reference diagnostic data were generated using the Bio-Rad HIV-1-2-O EIA/Western blot algorithm with further serotyping performed using the Multispot HIV-1/2 assay. (cdc.gov)
  • Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • However, similar levels of EP4 and FP receptor mRNA were observed comparing the fundus and LUS. (gla.ac.uk)
  • The functional roles of the two TNFRs in mediating NF-$\kappa$B activation, and inducing IL-2R$\alpha$ chain (CD25) and ICAM-1 (CD54) expression, were examined using receptor-specific antisera and TNF mutants, Northern blot analysis, gel shift assay and flow cytometry on human NK-like YT cells. (jefferson.edu)
  • Finally, a TNF triggered homoaggregation on YT cells was observed. (jefferson.edu)
  • We also observed a decrease in S6K1 phosphorylation in FNIP1- and, to a lesser extent, in FNIPL/FNIP2-suppressed cells. (nih.gov)
  • bone marrow cells, and the recovery of these lymphocyte subsets was observed when IRF-1? (tam-receptor.com)
  • In addition, RNA from THP-1 cells was used in Northern blots to determine whether inhibition of secretion of IL-1 beta and TNF-alpha by gemifloxacin occurred at the transcription or translation level. (illnesshacker.com)
  • Northern blot analysis of these cells revealed low to nondetectable levels of mRNAs for MHCencoded proteasome components LMP-7 and LMP-2, as well as the putative peptide transporters TAP-1 and TAP-2. (johnshopkins.edu)
  • Treatment of cells with interferon γ enhanced expression of these mRNAs and reversed the observed functional and biochemical deficits. (johnshopkins.edu)
  • Northern blot analysis showed that it was expressed predominantly in skeletal muscle. (aacrjournals.org)
  • Northern blot analysis showed that the size of the mRNA was about 2 kb, like that of the axi 1 isolated from tobacco. (tubitak.gov.tr)
  • P2XM was localized to chromosomal band 22q11, where frequent loss of heterozygosity has been observed in rhabdoid tumors. (aacrjournals.org)
  • In rat hepatocytes, no induction of CYP3A1 expression was observed following exposure to macrolides, even to erythromycin base and TAO (well known in vivo CYP3A1 inducers), whereas dexamethasone and phenobarbital were confirmed to induce this enzyme. (aspetjournals.org)
  • Expression studies by using both dotblot and Northern blot techniques revealed that human THEG is expressedspecifically in the testis. (embl-heidelberg.de)
  • They also observed an over-expression of haplotypes bearing CDSN*971T in a spontaneously immortalized HaCaT keratinocyte cell line. (dermatologytimes.com)
  • Expression of genes was quantified by Northern analysis as a ratio of the signal for the gene of interest to each of three housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase [GAPDH], beta-actin and cyclophilin). (gla.ac.uk)
  • 4 When expression of prostanoid receptor genes was studied in myometrium obtained from animals both in labour and not in labour and the techniques employed were optimized (principally the use of riboprobes), transcripts of similar size to the human genes were detected for prostanoid EP1, EP2, EP3, EP4, IP, FP and TP receptor mRNA using Northern blot. (gla.ac.uk)
  • 5 When expression of prostanoid receptor genes was studied in cervix obtained from animals both in labour and not in labour, clear signals which were similar in estimated size to the human genes were detected by Northern analysis for EP1, EP2, EP3, EP4, FP, IP and TP receptors. (gla.ac.uk)
  • Abnormal expression and mutational alterations of these receptors have been observed in several human malignancies. (nih.gov)
  • Expression of receptor proteins was examined by immunohistochemical analysis in paraffin-embedded prostatic tissue sections, and mRNA expression was determined by Northern blot and RT-PCR analysis. (nih.gov)
  • Finally, tissue-specific expression of the six mouse IGFBP genes was observed in adult tissues suggesting different roles or modes of actions in adult life. (eur.nl)
  • 3 Initial studies of animals not in labour using cDNA probes demonstrated transcripts of similar size to the human genes for prostanoid EP2, EP3, EP4, and FP receptor mRNA using Northern blot in myometrium. (gla.ac.uk)
  • HVL has not been reported) and 11 sites (2,086 serum samples) in the HVL-endemic northern region, where the disease was previously reported in 8 of 11 sites ( Figure ). (cdc.gov)
  • Although the concentrations of gemifloxacin used were higher than those observed in the serum of human volunteers treated with the dose under clinical development, it should be taken into consideration that concentrations at tissue and intracellular levels may be considerably higher than serum concentrations. (illnesshacker.com)
  • Cyclin D1 dysregulation and differential inactivation of p16INK4a and Rb have been observed in human lung cancer. (jax.org)
  • The t 1/2 value obtained for Nalm-6 is comparable to those estimated from Northern blot studies, using normal human leukocytes (5.5 h). (biomedcentral.com)
  • These extracts showed a marked reduction of signal on Northern blot analysis, suggesting nonsense-mediated decay of mutated transcripts, as is frequently observed in recessive disorders. (medscape.com)
  • By the employment of siRNA, we observed a decrease in S6K1 phosphorylation in the BHD-suppressed cell. (nih.gov)
  • Release from D-glucose repression of the β-mannanase genes was partial in the single deletants, while nearly full de-repression was observed in Δ creA Δ pkaA and Δ creA Δ ganB . (megazyme.com)
  • Northern blotting revealed that mIGFBP-2, -3, -4 and -5 genes are already expressed at gestational day 11.5, suggesting a role for these mIGFBPs in embryonal development. (eur.nl)
  • Northern blot analysis further revealed that this induction of IL-15 messenger RNA was impaired in IRF-1? (tam-receptor.com)
  • Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
  • For all five tested targets, we observed a consistent increase in modification efficiency with increasing tracrRNA length. (cdc.gov)
  • The results presented in this work indicate that the association CoLV/chloroplasts, observed in the ultrastructural studies, might be a casual event in the host cell, and that the virus does not replicate inside the organelle. (unesp.br)
  • At the 3' end of 10/3a, 63 % similarity at the nucleotide level was observed with the clone. (tubitak.gov.tr)
  • β-Xylanase production increased not only in Δ creA but also in Δ pkaA and Δ ganB , with the highest level observed in their double deletants, irrespective of the presence or absence of D-glucose. (megazyme.com)
  • Northern blots indicated that the inhibitory activity of gemifloxacin occurred post-transcription. (illnesshacker.com)
  • RNA was extracted and measured by Northern blot analysis using specific rat GIP probes. (duke.edu)
  • Due to alternative splicing within the coding regions, supplemental diversity is observed referred to as variable regions 1, 2, and 3 Firstly, variable region 1 (vr1) consists of a short exon containing three amino acids. (wikipedia.org)
  • weak or no bands were observed with negative samples. (cdc.gov)
  • The processing intermediates of microRNA precursors were analyzed by Northern blot and an increased accumulation of pre-microRNAs was found in the infected samples comparing to the non infected ones. (conicet.gov.ar)
  • Sequenceanalysis of these PCR products demonstrated that the smaller transcript(THEG minor) lacks 72 bp which was also observed for the mouse Theg. (embl-heidelberg.de)
  • No effect was observed in racial and/or rural disparities in stroke mortality. (cdc.gov)
  • i) the plant, animal or microorganism exhibits characteristics that were not previously observed in that plant, animal or microorganism. (canada.ca)
  • 3,4 The steadily increasing rate of this cancer in some provinces in the south has been observed in recent years. (who.int)
  • In this study, HVL seroprevalence in northern Israel was compared with that in a region free of clinical disease. (cdc.gov)