• This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3‐L1 adipocytes, as well as the mechanism involved. (researchgate.net)
  • 3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)
  • The S100 calcium binding protein B (S100B) implicated in brain inflammation acts via the receptor of advanced glycation end products (RAGE) and is also secreted from adipocytes. (nih.gov)
  • We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model. (nih.gov)
  • 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. (nih.gov)
  • In this study, with the assumption that adipose tissue can be mechanically responsive (as is the case for well-known musculoskeletal tissues), we hypothesized that mechanical loading applied to the adipose tissue and its component cells, such as adipocytes, may play a role in the exercise control of insulin sensitivity. (unl.edu)
  • As a proof-ofconcept test, we applied cyclic stretch loading to the adipocyte model, differentiated adipocytes from 3T3-L1 preadipocytes, and evaluated stretch-induced activation of key insulin signaling molecules. (unl.edu)
  • Here we report that antidiabetic thiazolidinediones (TZDs) and other ligands for the nuclear receptor PPARγ dramatically upregulate oxidized LDL receptor 1 (OLR1) in adipocytes by facilitating the exchange of coactivators for corepressors on the OLR1 gene in cultured mouse adipocytes. (jci.org)
  • Acer Tegmentosum Maxim Inhibits Adipogenesis in 3t3-l1 Adipocytes and " by Hang-Hee Cho, Soo-Jung Lee et al. (touro.edu)
  • Protein kinase B (Akt) and glycogen synthase kinase 3β (GSK3β) phosphorylation was also decreased by ATM treatment of 3T3-L1 adipocytes. (touro.edu)
  • In vitro treatment with Gal3 directly enhanced macrophage chemotaxis, reduced insulin-stimulated glucose uptake in myocytes and 3T3-L1 adipocytes and impaired insulin-mediated suppression of glucose output in primary mouse hepatocytes. (elsevierpure.com)
  • We investigated the effects of the estrogen receptor-α (ERα) and -β (ERβ) in the regulation of leptin, resistin, and adiponectin expression in 3T3-L1 adipocytes. (korea.ac.kr)
  • ERα induced leptin expression and ERβ inhibited its expression in 3T3-L1 adipocytes. (korea.ac.kr)
  • In addition, HPMFs also activated adenosine monophosphate-activated protein kinase (AMPK) signaling in 3T3-L1 adipocytes. (researchwithrutgers.com)
  • We found that cinnamon extract (CE) induced typical brown adipocyte multiocular phenotype in 3T3-L1 adipocytes. (edu.hk)
  • The treatment also increased brown adipocytes markers and reduced white adipocytes markers in the 3T3-L1 adipocytes. (edu.hk)
  • In ex vivo studies, we found that CE increased brown adipocytes markers in the subcutaneous adipocytes isolated from db/db mice and diet-induced obesity (DIO) mice. (edu.hk)
  • In IR adipocytes, uptake of D3 and 25-hydroxyvitamin-D3 was higher, but, after adrenaline stimulation, the decrement in D3 and 25-hydroxyvitamin-D3 was stronger in control cells, which also showed increased expression of Cyp27a1 and Cyp27b1 and higher levels of 25-hydroxyvitamin-D3. (medscape.com)
  • Here, we describe the cellular origin and physiological significance of micro LDs (mLDs) that emerge in the cytoplasm during active lipolysis, as well as the roles of key lipolytic proteins on mLDs in differentiated 3T3-L1 adipocytes. (indexindex.com)
  • Adipocytes represent a major cell type in the mammary tumor microenvironment and are important for tumor growth. (jci.org)
  • Methods 3T3‐L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. (researchgate.net)
  • We show that piceatannol inhibits adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner at noncytotoxic concentrations. (oregonstate.edu)
  • In the early phase of adipogenesis, piceatannol-treated preadipocytes displayed a delayed cell cycle entry into G(2)/M phase at 24 h after initiation of adipogenesis. (oregonstate.edu)
  • Main methods: Cell viability of 3T3-L1 preadipocytes was assessed by MTT assays, and lipid accumulation was measured by Oil Red O. Adipogenesis related factors were checked by Western blot analysis. (kist.re.kr)
  • In the early phase of adipogenesis, HPMF-treated preadipocytes displayed a delayed cell cycle entry into G 2 /M phase at 24 h (35.5% for DMI group and 4.8% for 20 μg/mL HPMFs-treated group) after initiation of adipogenesis. (researchwithrutgers.com)
  • Hirsutenone Directly Targets PI3K and ERK to Inhibit Adipogenesis in 3T3-L1 Preadipocytes. (oregonstate.edu)
  • Using Oil Red O staining, we observed that HST dose-dependently suppresses lipid accumulation during adipogenesis in 3T3-L1 preadipocytes, concomitant with a decrease in peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and fatty acid synthase (FAS) protein expression. (oregonstate.edu)
  • This inhibitory effect was largely limited to the early stage of adipogenesis, which includes mitotic clonal expansion (MCE), as evidenced by delayed cell cycle entry of preadipocytes from G1 to S phase. (oregonstate.edu)
  • Our results suggest that HST attenuates adipogenesis by directly targeting PI3K and ERK during MCE in 3T3-L1 preadipocytes, underscoring the potential therapeutic application of HST in preventing obesity. (oregonstate.edu)
  • A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. (oregonstate.edu)
  • Our objective was to determine the effects of 6,7,4'-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved. (oregonstate.edu)
  • Adipogenesis was stimulated in 3T3-L1 preadipocytes. (oregonstate.edu)
  • Our results suggest that 6,7,4'-THIF suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. (oregonstate.edu)
  • Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
  • PPARγ (Peroxisome proliferator-activated receptor gamma) agonists are well-known candidates for cytokine storm modulation. (bvsalud.org)
  • Further analysis indicates that OXPHOS attenuates the expression of IL-1ß, which suppresses the expression of peroxisome proliferator-activated receptor α (PPARα) and forkhead box O3 (FOXO3) in hepatocytes to suppress HBV gene expression and replication. (bvsalud.org)
  • Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. (foodandnutritionresearch.net)
  • This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. (antibodies-online.com)
  • Apigenin reduced CCAAT/enhancer binding protein (C/EBP) α and peroxisome proliferator-activated receptor γ levels. (kist.re.kr)
  • ATM (60 ug/mL) caused inhibition of adipogenesis via down-regulation of the CCAAT/enhancer binding protein β (C/EBPβ) (48%), C/EBPα (66%), and peroxisome proliferator-activated receptor γ (PPARγ) (64%) expressions in 3T3-L1 cells. (touro.edu)
  • Western blot analysis revealed that HPMFs markedly down-regulated adipogenesis-related transcription factors peroxisome proliferator-activated receptor (PPAR) γ and sterol regulatory element-binding protein (SREBP)-1c as well as downstream target fatty acid binding protein 2 (aP2), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC). (researchwithrutgers.com)
  • We observed that 6,7,4'-THIF, but not daidzein, inhibited MDI-induced adipogenesis significantly at 40 and 80 μM, associated with decreased peroxisome proliferator-activated receptor-γ and C/EBP-α protein expression. (oregonstate.edu)
  • BACKGROUND: Peroxisome proliferator-activated receptor gamma (PPAR-γ) which controls body weight, glucose homeostasis, and adipocyte differentiation is a valuable candidate gene for insulin resistance (IR). (ac.ir)
  • OLR1 expression in white adipose tissue is increased in obesity and is further induced by PPARγ ligand treatment in vivo. (jci.org)
  • Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. (antibodies-online.com)
  • This was associated with DIM‐enhanced phosphorylation of the signaling intermediates Akt, insulin receptor substrate‐1, and insulin receptor early in differentiation. (researchgate.net)
  • Piceatannol, natural polyphenolic stilbene, inhibits adipogenesis via modulation of mitotic clonal expansion and insulin receptor-dependent insulin signaling in early phase of differentiation. (oregonstate.edu)
  • Piceatannol dose-dependently inhibited differentiation mixture-induced phosphorylation of insulin receptor (IR)/insulin receptor substrate-1 (IRS-1)/Akt pathway in the early phase of adipogenesis. (oregonstate.edu)
  • Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. (foodandnutritionresearch.net)
  • Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
  • MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
  • Key findings: Among five flavonoids isolated from GFF, only apigenin potently inhibited the differentiation of 3T3-L1 cells. (kist.re.kr)
  • Apigenin-treated 3T3-L1 cells failed to undergo clonal expansion during the early phase of adipocyte differentiation. (kist.re.kr)
  • Significance: These findings suggest that apigenin can prevent 3T3-L1 preadipocyte differentiation by the inhibition of the mitotic clonal expansion and the adipogenesis related factors and upregulation of the expression of multiple C/EBPβ inhibitors. (kist.re.kr)
  • We investigated the effect of Acer tegmentosum Maxim (ATM) on adipocyte differentiation in 3T3-L1 cells and anti-obesity properties in obese rats fed a high-fat diet (HFD). (touro.edu)
  • Thus any modification of ECM components can be transmitted via integrins and impact on cell movement during development and proliferation, differentiation, apoptosis. (springer.com)
  • Cellular lipid content in DMI (dexamethasone, 3-isobutyl-1-methylxanthine, and insulin mixture)-treated cells increased, while ATM treatment caused a significant reduction in lipid accumulation in differentiated 3T3-L1 cells. (touro.edu)
  • the effects of hypoxia and fibrosis may also play a role in obesity complications via notably ectopic-lipid accumulation in non-adipocyte cells. (springer.com)
  • However, lipid accumulation in non-specialized cells is deleterious and can contribute to cell death resulting in the development of pathology such as NASH (Non-Alcoholic Steatohepatitis) a liver injury associated with obesity [ 12 ]. (springer.com)
  • 6,7,4'-THIF significantly suppressed MDI-induced lipid accumulation in the early stage of adipogenesis, attributable to a suppression of cell proliferation and the induction of cell cycle arrest. (oregonstate.edu)
  • Using the network pharmacology approach, we identified two clusters of genes involved in immune response (IL1A, IL2, and IL6R) and cell proliferation (CCND1, PPARG, and EGFR) mediated by biochanin A in CRC/COVID-19 condition. (bvsalud.org)
  • abstract = "Browning is the process of increasing the number of brite cells, which helps to increase energy expenditure and reduce obesity. (edu.hk)
  • Flow cytometry was used to analyze the mitotic cell cycle during the mitotic clonal expansion phase. (kist.re.kr)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • Type 1 diabetes mellitus is a metabolic disease resulting from the destruction of insulinproducing β cells in the pancreas, that leads to hyperglycemia [1,2,20]. (researchgate.net)
  • The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis, non-alcoholic fatty liver disease (NAFLD) and an independent risk factor for metabolic syndrome. (wikipedia.org)
  • Expression of the receptors is correlated with insulin levels, as well as reduced in mouse models of diabetes, particularly in skeletal muscle and adipose tissue. (wikipedia.org)
  • Obesity often increases the risk of lifestyle diseases, such as cardiovascular disease, diabetes, and cancer. (frontiersin.org)
  • There is a connection between obesity and metabolic disorders such as diabetes, high blood pressure, and high cholesterol levels. (nordicoil.com)
  • Obesity can be viewed as a state of long-term lipid disequilibrium that is marked by massive adipocyte hypertrophy and is a major risk factor for developing insulin resistance and type 2 diabetes. (jci.org)
  • Their potential applications for the treatment of endotoxaemia, obesity, diabetes, Alzheimer's disease and their uses as cardiovascular and gastrointestinal protective agents, analgesics, anti-oxidant, anti-atherosclerosis agents, dermatological agents and anti-infectious agents are highlighted. (biomedcentral.com)
  • Obesity is a prominent risk factor for Non-insulin Dependent Diabetes Mellitus, (NIDDM), which is also called diabetes mellitus type II. (heraldopenaccess.us)
  • Obesity is defined as an expansion of white adipose (WAT) tissue that is associated with low grade inflammation which possibly contributes to the development of health complications such as diabetes, liver pathologies, dyslipidemia, cardiovascular diseases as well as some cancers [ 2 - 4 ]. (springer.com)
  • We sought to assess whether the flavonoid-rich fraction of crude extracts from Daphne genkwa Siebold et Zuccarini (GFF) might inhibit adipogenesis of 3T3-L1 cells. (kist.re.kr)
  • Importantly, we found that Gal3 can bind directly to the insulin receptor (IR) and inhibit downstream IR signaling. (elsevierpure.com)
  • It was observed that expressions of insulin receptor substrate-1 (IRS-1) and glucose transport-4 (GLUT4) were less influenced by stretch under the given conditions. (unl.edu)
  • One theory suggests that lipid over supply leads to defect in Insulin Receptor Substrate (IRS) through serine/threonine phosphorylation. (heraldopenaccess.us)
  • As a critical node for insulin/IGF signaling, insulin receptor substrate 1 (IRS-1) is essential for metabolic regulation. (nature.com)
  • Insulin receptor substrate (IRS) proteins play essential roles in mediating insulin/IGF signaling by recruiting a series of downstream effectors 23 . (nature.com)
  • Adiponectin binds to a number of receptors. (wikipedia.org)
  • So far, two receptors have been identified with homology to G protein-coupled receptors, and one receptor similar to the cadherin family: Adiponectin receptor 1 (AdipoR1) Adiponectin receptor 2 (AdipoR2) T-cadherin - CDH13 These have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. (wikipedia.org)
  • Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)
  • We investigated the anti-obesity effects of ATM on HFD-induced obese rats. (touro.edu)
  • in both IR cells and obese SAT, protein expression of β 2 -adrenergic receptor was reduced. (medscape.com)
  • The high prevalence of vitamin D deficiency in obese subjects [ 3 ] suggests that adipose tissue (AT) has a major role in the body distribution of vitamin D, but the causal relationship between obesity and low levels of circulating 25(OH)D has not been completely elucidated yet. (medscape.com)
  • DIM, a major metabolite of indole-3-carbinol, which is naturally produced in broccoli and cabbage, enhances glucose uptake through the improvement of insulin sensitivity in 3T3-L1 cells [13] . (researchgate.net)
  • In this review, selected foods and their components with potential anti-obesity properties are discussed. (scirp.org)
  • In addition, flu infection reduced PPARγ expression in all mouse macrophages, including AMs, interstitial macrophages, and bone-marrow-derived macrophages but not in alveolar epithelial cells. (bvsalud.org)
  • Cells infected with MA104 rotavirus and/or transfected with plasmids expressing NSP proteins, were analyzed for expression of cellular proteins related to NFκB and PPARγ pathways and evaluated through the ELISA, luminescence, flow cytometry and Western blot techniques. (bvsalud.org)
  • However, Ucp2 displays a wide tissue expression range ( 5 ), and its roles in various tissues and cell types have not been covered. (bmbreports.org)
  • The co-culture of RAW and L1 increased TNF-α, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. (nih.gov)
  • In addition, apigenin inhibited the DNA-binding activity of C/EBPβ in differentiating 3T3-L1 cells by down-regulating the 35 kDa isoform of C/EBPβ (liver-enriched activating protein) and up-regulating the expression of two different sets of C/EBP inhibitors: C/EBP homologous protein and the phospho-liver-enriched inhibitory protein isoform of C/EBPβ. (kist.re.kr)
  • To clarify the expression and regulation of adipokines by ER subtypes, total RNA was extracted from cells and measured using quantitative PCR. (korea.ac.kr)
  • β3-adernergic receptor (β3-AR) antagonist reduced the CE-enhanced UCP1 expression, suggesting an involvement of the β3-AR activity. (edu.hk)
  • In the present study, the effect of MSG on cell viability and its effect on expression of APC , BECN1 , and TP53 genes in SW620 and SW480 colon cancer cell lines were studied. (scielo.br)
  • conversely, TP53 showed a significant decrease in expression in SW620 cells. (scielo.br)
  • expression of 25-hydroxylase ( Cyp27a1 ), 1 α -hydroxylase ( Cyp27b1 ), and vitamin D receptor ( Vdr ) was analyzed by real-time polymerase chain reaction. (medscape.com)
  • Obesity and related metabolic disorders have reached global epidemic proportions in recent decades. (unl.edu)
  • CBD was able to block the CB1 receptors according to a study [4] and thus influence the risk of obesity and metabolic disorders. (nordicoil.com)
  • S100B upregulated TNF-α and M1 markers in RAW, and TNF-α augmented S100B secretion from L1. (nih.gov)
  • L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. (nih.gov)
  • The number of mitochondria is increased when ingested, proteins that accelerate fat breakdown are stimulated, and proteins that produce fat cells are blocked. (nordicoil.com)
  • Time-lapse imaging of GFP-tagged LD-associated proteins and immunocytochemical analyses showed that particulate structures carrying LD-associated proteins emerged throughout the cells upon lipolytic stimulation, but not when FA re-esterification was blocked. (indexindex.com)
  • The expanding problem of obesity is becoming a very serious issue in developed and even developing societies. (heraldopenaccess.us)
  • Davidson, T.L. and Swithers, S.E. (2004) A Pavlovian Approach to the Problem of Obesity. (scirp.org)
  • Thus, there is a tendency to use natural compounds for the management of obesity. (mdpi.com)
  • Changes in life-style along with modifications to the diet are important in the management of obesity. (scirp.org)
  • Despite a well documented health benefit of resveratrol in intervention of the development of obesity, the role of piceatannol in the development of adipose tissue and related diseases is unknown. (oregonstate.edu)
  • A differential response in the development of obesity complications exists depending on WAT depot distribution. (springer.com)
  • Cancer cell viability was analyzed using MTT assay, and the results showed a significant increase in the number of viable cells after 24 h of treatment with MSG with different concentrations (0.5, 1.0, 10, 50, and 100mM). (scielo.br)
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • TP53 is a tumor suppressor gene that functions in modifying DNA errors and/or inducing apoptosis of damaged cells, and both APC and BECN1 genes are involved in CRC and are of importance in cellular growth and metastasis. (scielo.br)
  • Now focus has been geared towards the development of foods that possess more than one mechanism to alter the progression of obesity. (scirp.org)
  • F. H. Chen exhibits anti-obesity effects. (frontiersin.org)
  • Although notoginsenosides are potential drugs for the treatment of obesity, their effects and mechanisms have not been analyzed in depth. (frontiersin.org)
  • Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. (foodandnutritionresearch.net)
  • Synthetic anti-obesity drugs show side effects and variable effectiveness. (mdpi.com)
  • in addition, obesity increases the production of IL-1β, which may have cytotoxic effects on beta islet cells [7]. (heraldopenaccess.us)
  • Positive effects in managing obesity by natural components, and selected foods have drawn attention due to the potential side effects of obesity drugs. (scirp.org)
  • These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)
  • Concentrations of cholesterol and triglycerides are strongly correlated in the adipocyte, but little is known about mechanisms regulating cholesterol metabolism in fat cells. (jci.org)
  • Vitamin D is a lipophilic hormone playing a key role in bone metabolism and calcium homeostasis, [ 1 ] and it is also involved in many other pathological processes [ 2 ] commonly linked with obesity. (medscape.com)
  • Insulin resistance increases the risk of building up white fat cells and damaging the organs. (nordicoil.com)
  • Depression and obesity are strongly interrelated: The occurrence of one increases the risk of the other [4]. (heraldopenaccess.us)
  • However, currently, there are very few drugs available for obesity treatment. (frontiersin.org)
  • Soy isoflavones like 6,7,4'-THIF may have potential for development into novel treatment strategies for chronic obesity. (oregonstate.edu)
  • PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. (antibodies-online.com)
  • The receptors affect the downstream target AMP kinase, an important cellular metabolic rate control point. (wikipedia.org)
  • In this study, we observed the overexpression of SUMO-speci﫿c protease 2 (Senp2) in adipose tissues during obesity. (deepdyve.com)