Nucleotide oligonucleotide array sequence analysis. Medical search. Frequent questions

Combined analyses including FISH, coding sequence analysis, high-density single nucleotide polymorphism-based oligonucleotide arrays, and multiplex ligation-dependent probe amplification enable the identification of biallelic, inactivating perturbations of SMARCB1 in nearly all MRTs, consistent with the 2-hit model of tumor formation. (medscape.com)
Despite technical issues regarding design, data analysis and validation that have to be addressed in the planning and interpretation of a microarray study, microarray-based approaches for studying transcript expression, single-nucleotide-polymorphism genotyping and gene resequencing are becoming more widely adopted. (medscape.com)
Although restriction fragment length polymorphism analysis remains a widely used method for detecting DNA sequence polymorphism, several useful variations on the fragment length theme have recently been introduced. (justia.com)
The expression of ACE gene was detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis in 36 Algerian patients with glioma and 195 healthy controls. (cancer-genetics.org)
Recently developed microarray technology, which refers to attachment of short oligonucleotides on a solid/polymeric surface, has proved to be useful for screening of genetic mutations, study of polymorphism, as diagnostics, etc. (researchgate.net)
Furthermore, single nucleotide polymorphism (SNP) detection was examined using the oligonucleotide-arrayed TFT photosensor. (elsevierpure.com)
Single-nucleotide polymorphism (SNP) analysis is a powerful tool for mapping and diagnosing disease-related alleles. (uni-koeln.de)

Increasingly, this question is being addressed through the simultaneous analysis of hundreds to thousands of unique genetic elements with microarrays. (biomedcentral.com)
Presently, two technologies dominate the field of high-density microarrays: cDNA arrays and oligonucleotide arrays. (biomedcentral.com)
Relative to conventional two- dimensional microarrays , the bead arrays have larger surface- to- volume ratios, providing faster hybridization kinetics, and they also offer much more flexibility and scalability in assay design. (genomicglossaries.com)
Small scale specialty or 'boutique' array, where large proportions of the genes on the microarrays are expected to be highly differentially expressed. (genomicglossaries.com)
spotted cDNA microarrays , spotted oligonucleotide microarrays , and Affymetrix GeneChips , which are sufficiently unique to warrant their own grouping. (genomicglossaries.com)
The general categories we describe are macroarrays, microarrays , high- density oligonucleotide array s (e.g. (genomicglossaries.com)
Massively parallel experimental assays, particularly DNA microarrays and sequencing, have revolutionized genomics research in the last decades. (nature.com)
Direct syntheses (in situ) of oligonucleotides on the chip surface following conventional or photolithographic methods and immobilization of presynthesized oligonucleotides on selected substrates (the deposition method) are two predominant methods for fabrication of oligonucleotide microarrays (6)(7) (8) (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). (researchgate.net)
The in situ synthesis protocol, which allows the preparation of high-density oligonucleotide microarrays, suffers from certain drawbacks (6)(7)(8)17). (researchgate.net)
Microarrays allow the quantitative analysis of thousands of gene expression changes simultaneously in a single experiment, orders of magnitude more than the number that could be evaluated using older technologies for quantitative gene expression [i.e. (nih.gov)
The advantage of microarray (array) over conventional karyotype for the diagnosis of fetal pathogenic chromosomal anomalies has prompted the use of microarrays in prenatal diagnostics. (mdpi.com)
Gene detection and the quantification of gene expression in samples require the use of oligonucleotide microarrays. (dead-samurai.com)
Microarrays are key to in-depth analyses necessary during pharmaceutical research. (dead-samurai.com)

Most current methods for inferring ancestry at each locus in the genome use a few thousand single nucleotide polymorphisms (SNPs) that are very different in frequency between West Africans and European Americans, and that are required to not be in linkage disequilibrium in the ancestral populations. (nih.gov)
Unbiased genetic association studies, including genome-wide association and whole-genome sequencing studies, have uncovered many novel disease-associated variants. (nih.gov)
The advent of affordable benchtop next-generation sequencing platforms such as the semiconductor-based Ion Personal Genome Machine (PGM) Sequencer has facilitated multi-gene mutational profiling using only nanograms of DNA. (nih.gov)
Sequencing was carried out at the Baylor Genome Center (U54 HG003273). (cam.ac.uk)
The ability to perform large-scale analysis of the genome at the level of gene sequence, gene copy number and messenger RNA transcript expression characterizes the post-genomic era. (medscape.com)
An oligonucleotide microarray for high-throughput sequencing of the mitochondrial genome. (nih.gov)
Here we report the development of a second-generation MitoChip (v2.0) with oligonucleotide probes to sequence the entire mitochondrial genome. (nih.gov)
the goal of this initiative is to reduce costs by at least four orders of magnitude, so that a mammalian-sized genome could be sequenced for approximately $1000. (nih.gov)
Multiple degenerate capture and intermediate oligonucleotides (usually four or five, respectively) complementary to the conserved regions covering the entire genome of each subtype were designed. (cdc.gov)
In this review we compare the performance of different array platforms (BAC, oligonucleotide CGH, SNP) and designs (targeted, whole genome, whole genome, and targeted, custom) and discuss their advantages and disadvantages in relation to prenatal testing. (mdpi.com)
Nevertheless, spurred by technological improvements in the ability the perform bioassays in a massively parallel fashion, the sequencing of the human genome, and the development of statistical methodologies, researchers now have the capacity to leverage information from other levels of the system to raised understand the function of genetic perturbations in disease. (biopaqc.com)
Understanding as much information as possible about the human genome sequence increases the results of drug creation and use. (dead-samurai.com)
Major research efforts in the field include sequence alignment, gene finding, genome assembly, drug design, drug discovery, protein structure alignment, protein structure prediction, prediction of gene expression and protein-protein interactions, genome-wide association studies, and the modeling of evolution. (limsforum.com)
First, the mouse tsg101 cDNA sequence was used to query the Expressed Sequences Tags (ESTs) database of the National Center for Biotechnology Information (NCBI) by the BLAST program in order to find homologous sequences in the human genome. (davidson.edu)
To understand the underlying genetic changes that may be responsible for this evolution, we performed a comprehensive genome-level analyses of 4 M. bovis strains that encompass the main molecular types of the pathogen circulating in GB. (aber.ac.uk)
Results: We have used a combination of genome sequencing, transcriptome analyses, and recombinant DNA technology to define genetic differences across the major M. bovis lineages circulating in GB that may give rise to phenotypic differences of practical importance. (aber.ac.uk)
The genomes of three M. bovis field isolates were sequenced using Illumina sequencing technology and strain specific differences in gene expression were measured during in vitro growth and in ex vivo bovine alveolar macrophages using a whole genome amplicon microarray and a whole genome tiled oligonucleotide microarray. (aber.ac.uk)

These arrays can contain hundreds of thousands to millions of individual probes , making them perhaps the most promising technology for the vast number of tests that will be needed for applications such as drug discovery and for diagnosis and treatment of patients. (genomicglossaries.com)
The immobilization of thiolated oligonucleotide probes onto a Ti0 2 -coated TFT photosensor using γ-aminopropyltriethoxysilane (APTES) and N-(γ-maleimidobutyloxy) sulfosuccinimide ester (GMBS) was optimized. (elsevierpure.com)
AIM: A rapid analysis of mitochondrial DNA (mtDNA) sequences with an array of immobilized sequence-specific oligonucleotide (SSO) probes was tested on 18 skeletal elements recovered from mass graves in Croatia, which could not be genotyped with common forensic nuclear DNA systems (PM+DQA1 and short tandem repeat analysis). (lookformedical.com)
However, the number of molecules hybridized to the probes is generally lower than the amount necessary for efficient application of some next generation sequencing chemistries. (justia.com)
In preferred embodiments an array of probes complementary to a plurality of sequences of interest are hybridized to targets. (justia.com)

cDNA arrays A microscopic, ordered array of nucleic acids, proteins, small molecules , cells or other substances that enables parallel analysis of complex biochemical samples. (genomicglossaries.com)
Alignment of the putative human and mouse TSG101 proteins (Figure 1B) shows 94% identity between the protein sequences, with 20 amino acid mismatches and one gap. (davidson.edu)
N.C. Seeman, J.M. Rosenberg and A. Rich, Sequence Specific Recognition of Double Helical Nucleic Acids by Proteins. (nyu.edu)

[3] [4] Its primary use since at least the late 1980s has been in genomics and genetics, particularly in those areas of genomics involving large-scale DNA sequencing . (limsforum.com)

Modern SNP arrays provide data on hundreds of thousands of SNPs per sample, and to use these to infer ancestry, using many of the standard methods, it is necessary to choose subsets of the SNPs for analysis. (nih.gov)
Here we present panels of about 4,300 ancestry informative markers (AIMs) that are subsets respectively of SNPs on the Illumina 1 M, Illumina 650, Illumina 610, Affymetrix 6.0 and Affymetrix 5.0 arrays. (nih.gov)
To identify single-nucleotide polymorphisms (SNPs) associated with risk and age at onset of Alzheimer disease (AD) in a genomewide association study of 469 438 SNPs. (nih.gov)
METHODS: We used a novel gene chip containing 48,742 single-nucleotide polymorphisms (SNPs) in 2100 candidate genes to test for associations in 3145 case subjects with coronary disease and 3352 control subjects. (ox.ac.uk)
A meta-analysis showed that with a genotype score involving both LPA SNPs, the odds ratios for coronary disease were 1.51 (95% CI, 1.38 to 1.66) for one variant and 2.57 (95% CI, 1.80 to 3.67) for two or more variants. (ox.ac.uk)

Conduct of PCR with genomic DNA using single short (8-10 mer) primers of arbitrary sequence generates a product that can be used in gel electrophoretic fingerprint analysis to generate numerous polymorphic markers (6,7). (justia.com)
To evaluate capture oligonucleotides in the nanomicroarray assay, PCR primers sets for amplification of HA, NA, and M genes of H2N2, H7N2, H7N3 and H9N2 were designed and are listed in Technical Appendix Table 1. (cdc.gov)
Oligonucleotide primers were made based on a region of identity between two of the ESTs. (davidson.edu)
With these primers and others corresponding to sequences bracketing the Lambda gt10 cloning site of a human cDNA library, the human TSG101 cDNA was amplified by PCR (Figure 1Ab). (davidson.edu)
Oligonucleotide primers for the reaction are automatically selected by the software, a unique DNA tag/anti-tag system is generated, and the pairing of primers and DNA-tags is automatically done in a way to avoid any crossreactivity. (uni-koeln.de)
Two oligonucleotide primers each hybridize to their respective complementary DNA strands in the second step (annealing step), thus defining a region of the target DNA. (distantreader.org)

Here, we combined high-resolution array-based comparative genomic hybridization (aCGH) with gene expression profiling to target genes within focal high-level amplifications in GC cell lines, and identified RAB23 as an amplified and overexpressed Chr 6p11p12 gene in Hs746T cells. (duke.edu)
All tested samples underwent successful targeted sequencing of 46 genes. (nih.gov)
Analysis of DNA polymorphisms has been extremely valuable for identifying genetic markers tightly linked to genes associated with phenotypic traits. (justia.com)
120 known sequences of HA and NA genes of different subtypes were selected from 500 hit count analyses of each gene covering sequences of viruses from different geographic regions (i.e. (cdc.gov)
This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. (cancer-genetics.org)
A molecular biologist may use an array scanner to measure how specific conditions influence the production of mRNA within a set of genes. (dead-samurai.com)
Bioinformatics is limited to sequence, structural, and functional analysis of genes and genomes and their corresponding products and is often considered computational molecular biology. (limsforum.com)

Methods for solid phase clonal amplification of hybridized target molecules on the surface of the arrays or short oligonucleotides of know sequence and location are disclosed. (justia.com)

Use of next-generation sequencing also enhances the power of fine needle aspiration by providing gene mutation results that can direct personalized cancer therapy. (nih.gov)
A combined linkage, microarray and exome analysis suggests MAP3K11 as a candidate gene for left ventricular hypertrophy. (ox.ac.uk)
This critical design ensures that the target gene is captured on the microarray, hybridized with intermediate oligonucleotides and detected using the gold nanoparticle probe. (cdc.gov)
In addition, a universal primer set previously reported to amplify all eight gene segments was modified by adding a 13 bp in length of flanking sequence (5-ACGACGGGCGACA-3) at the 5 end of each primer to enhance the annealing temperature and achieve high fidelity and yield in PCR amplification. (cdc.gov)
According to the available TEM and SHV genotypes, we designed a gene chip that can detect most of the genotypes of TEM and SHV-ESBLs in clinical isolates, because gene chip has the advantage of detecting single nucleotide mutation. (ncl.edu.tw)
DNA microarray technology aids in gene expression analysis and other bioinformatics functions. (limsforum.com)
The mouse gene, tsg101, appears to suppress malignant cell growth, and the putative protein encoded by tsg 101 has the sequence characteristics of a transcription factor. (davidson.edu)
A query of the NCBI database returned no human gene sequences that had significant homology to the TSG101 gene, supporting the notion that the gene is a unique human homologue to the mouse tsg101. (davidson.edu)
Mouse-rodent hybrid cells were used as a source of template DNA for PCR analysis, which localized the gene to mouse chromosome 7. (davidson.edu)
SNP/small base pair insertion and deletions and gene expression data were overlaid onto the genomic sequence of the fully sequenced strain of M. bovis 2122/97 to link observed strain specific genomic differences with differences in RNA expression. (aber.ac.uk)
Furthermore, we show that transcriptomic data based solely on amplicon arrays can generate spurious results in terms of gene expression profiles due to hybridisation of antisense transcripts. (aber.ac.uk)

The existence of variable number tandem repeat (VNTR) or "microsatellite" sequences scattered throughout genomic DNA has been exploited in the identification of polymorphic markers (2,3). (justia.com)
This Funding Opportunity Announcement (FOA) solicits Small Business Innovation Research (SBIR) grant applications from small business concerns (SBCs) to develop novel technologies that will enable extremely low-cost genomic DNA sequencing. (nih.gov)
We provide genomic, transcriptomic and functional data to show that synonymous point mutations (sSNPs) on the coding strand can lead to the expression of antisense transcripts on the opposing strand, a finding with implications for how we define a 'silent' nucleotide change. (aber.ac.uk)

After the DNA library is sequenced on the Illumina flow cell, it is enzymatically double stranded by extension of a 5′ biotinylated primer. (nature.com)

Quantitative analysis of RNA on a massively parallel array (RNA-MaP) 21 , 23 starts with ssDNA sequencing libraries including an RNA polymerase promoter region, a stall sequence, and a variable region complementary to the desired RNA. (nature.com)
High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)
METHODS: We used duplex polymerase chain reaction (PCR) amplification of the mtDNA hypervariable regions I and II (HVI and HVII) (444 bp and 415 bp amplicons, respectively) and subsequent linear array typing, which targets six polymorphic regions and two additional sites within the human mtDNA HVI and HVII. (lookformedical.com)
Mutation analysis by polymerase mediated single-base primer extension (minisequencing) can be massively parallelized using DNA microchips or flow cytometry with microspheres as solid support. (uni-koeln.de)
Batt, C. A. title: Applications of DNA amplification techniques in veterinary diagnostics date: 1995 journal: Vet Res Commun DOI: 10.1007/bf01839319 sha: 9282b6f2a3a4c79ab56505e5c040a8b94b0750de doc_id: 5364 cord_uid: gwkr4r1k An overview of the principles of the polymerase chain reaction, ligase chain reaction, self-sustained sequence replication and Qβ replicase is given. (distantreader.org)
The polymerase chain reaction (PCR) is an in vitro enzymatic method which allows several million-fold amplification of a specific DNA sequence. (distantreader.org)

Here, we demonstrate a protocol that employs coating of SU-8 (glycidyl ether of bisphenol A) onto glass microslides to obtain high density of epoxy functions for efficient immobilization of aminoalkyl-, thiophosphoryl-, and phosphorylated oligonucleotides with uniform spot morphology. (researchgate.net)

Background: Methotrexate (MTX) is an inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in nucleotide synthesis, and is widely used in therapy, but its effi cacy is often compromised by the development of resistance in cancer cells. (medscimonit.com)
The designed intermediate oligonucleotides modified with 5-amino-C6-modifier while a 25-mer poly (A) tail were added at the 3 end of intermediate oligonucleotides during synthesis (Integrated DNA Technologies, Coralville, IA, USA). (cdc.gov)
Using Affymetrix GENECHIP arrays, specific hybridization can be achieved by direct synthesis of the DNA sequences of interest on arrays. (justia.com)
The amplified target is then analyzed, for example, by sequencing by synthesis or sequencing by ligation. (justia.com)
RNA sequences that serve as templates for protein synthesis. (lookformedical.com)

In this assay, based on the Luminex xMAP system, carefully selected oligonucleotides were covalently linked to fluorescently coded microspheres that are hybridized to biotinylated cRNA followed by amplification of the signal, which results in a rapid, sensitive, multiplexed assay platform. (nih.gov)

The selected nucleotide sequences were further aligned to identify significant divergent or conserved regions for detection and differentiation of subtypes. (cdc.gov)
Microarray technology has become an important tool for detection and analysis of nucleic acid targets. (researchgate.net)
DNA hybridization in the presence of both ALDH2*1 and ALDH2*2 target DNA was observed using both ALDH2*1 and ALDH2*2 detection oligonucleotides-arrayed TFT photosensor. (elsevierpure.com)

Affymetrix s GeneChips ), and microelectronic arrays . (genomicglossaries.com)

Short synthetic oligonucleotides are finding wide variety of applications in area of genomics and medicinal chemistry. (researchgate.net)
Combining whole transcriptome analysis with morphological context, spatial transcriptomics allows researchers to uncover the "where" for every "what," according to 10X Genomics. (genengnews.com)
For example, over the years, AGBT meetings have highlighted the launches of next-generation sequencing stars such as Complete Genomics, Pacific Biosciences, Ion Torrent, and Oxford Nanopore. (genengnews.com)

The DNA mixtures were denatured and reannealed, and the resultant homo- and heteroduplices were evaluated by temperature-modulated heteroduplex analysis by the DHPLC method. (lookformedical.com)

Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. (justia.com)
In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. (justia.com)
The methods of the invention relate generally to amplification of nucleic acid on a solid support and analysis of the amplified nucleic acid. (justia.com)
The methods disclosed herein enable solid phase clonal amplification of hybridized target molecules on the surface of the arrays, such as GENECHIP arrays, after hybridization. (justia.com)
Then, the longest 5' and 3' fragments obtained from PCR amplification were joined into the pAMP1 cloning vector and sequenced. (davidson.edu)
Other recently developed DNA amplification methods, such as the ligase chain reaction (LCR), self-sustained sequence replication (3SR) and Qfl replicase amplification have so far found only limited applications, but show promise for specific diagnostic applications. (distantreader.org)
Amplification of RNA sequences, e.g. (distantreader.org)

We showed 100% concordance of results between next-generation sequencing and conventional test platforms for all previously known point mutations that included BRAF, EGFR, KRAS, MET, NRAS, PIK3CA, RET and TP53, deletions of EGFR and wild-type calls. (nih.gov)
In addition, the MitoChip v2.0 contains redundant tiling of sequences for 500 of the most common haplotypes including single-nucleotide changes, insertions, and deletions. (nih.gov)

The most frequent type of genetic change associated with an RFLP marker is point mutation within the recognition sequence of a restriction enzyme. (justia.com)
Several mutations have been described, but when DNA analysis is proposed as a screening test for morbidity and mortality associated with iron overload in the setting of the general adult population, the only mutation of interest is C282Y. (cdc.gov)

Cyclic mono- or oligonucleotide second messenger molecules are ubiquitously occurring in cells from all three domains of life and are implicated in a wide array of signaling networks at the single or multiple cell level. (nature.com)

Multiple coding region variants detected only in the second generation MitoChips, but not the earlier chip version, were further confirmed with conventional sequencing. (nih.gov)

However, many challenges of the technology remain -- quality control, expression arrays and pharmacokinetics will be addressed as well as new developments in the field, such as microRNA arrays and DNA methylation. (genomicglossaries.com)

Sequencing results from 14 primary head and neck tumor tissues demonstrated that the v2.0 MitoChips detected a larger number of variants than the original version. (nih.gov)
Accurate identification of disease-causing sequence variants and driving pathways is essential to minimize drug resistance or tumor relapse with targeted therapeutics [ 15 ]. (biomedcentral.com)
Because the anatomic pathologist's analysis of tissue from a patient's tumor is absolutely critical in sarcoma treatment, sarcoma pathologists are specially trained in the diagnosis of these rare tumors. (sarcomahelp.org)

Analysis of microbial populations, important in environmental and industrial settings, is currently a daunting task, typically requiring extensive culturing and a battery of biochemical tests, supplemented by crude classification by visual inspection. (justia.com)
Such clonally amplified DNAs can serve as a template for next generation sequencing biochemical reactions. (justia.com)

Using single-cell RNA sequencing (RNA-seq), we examine the intratumoral heterogeneity of a pair of primary renal cell carcinoma and its lung metastasis. (biomedcentral.com)

Previously we developed an oligonucleotide sequencing microarray (MitoChip) as an array-based sequencing platform for rapid and high-throughput analysis of mitochondrial DNA. (nih.gov)
Forensic utility of mitochondrial DNA analysis based on denaturing high-performance liquid chromatography. (lookformedical.com)

This approach allows for the locus specific targeting of sequencing in only regions of interest and reduces the overwhelming redundancies required with other random sampling methods. (justia.com)
Methods for the locus specific targeting of sequencing or analysis of regions of interest are disclosed. (justia.com)

Current DNA array formats can be categorized into various groups based on the type of matrix, the probe number and/or density , the physical size of the array, and the type of target labeling. (genomicglossaries.com)
The prominent feature of the protocol is that spots can be visualized distinctly at 0.05 μM probe (a 20-mer oligonucleotide) concentration. (researchgate.net)
Identification of human remains by immobilized sequence-specific oligonucleotide probe analysis of mtDNA hypervariable regions I and II. (lookformedical.com)

They are used as miniaturized platforms for analysis of nucleic acids-based biomarkers. (mdpi.com)

Here we explore the role of coding variation on these traits by analysis of variants on the HumanExome BeadChip in 60,564 non-diabetic individuals and in 16,491 T2D cases and 81,877 controls. (cam.ac.uk)
We combined linkage and association analyses to discover novel rare genetic variants involved in three such measures and two principal components derived from them. (ox.ac.uk)
Exome sequence data identified two associated variants after multiple testing corrections were applied. (ox.ac.uk)

Another recent advance in polymorphic marker analysis is single short primer PCR, or "random amplified polymorphic DNA" (RAPD) marker analysis. (justia.com)
Presently the commercial offerings range from systems that claim to achieve from 40 to 100 MB of sequence information in a single run, up to about 1 to almost 2 GB of assembled sequences in a single run. (justia.com)
The targets of interest are then amplified in a defined location so that many copies of the same target sequence are generated in a single location. (justia.com)

Therefore, the field of bioinformatics has evolved such that the most pressing task now involves the analysis and interpretation of various types of data, including nucleotide and amino acid sequences, protein domains, and protein structures. (limsforum.com)
An open reading frame of 1140 nucleotides found in the cDNA sequence was predicted to encode a 380 amino acid protein with a mass of 42.841 kDa. (davidson.edu)
In addition, the human TSG101 amino acid sequence also contains seven possible protein kinase C phosphorylation sites, five possible casein kinase II phosphorylation sites, and three possible N-glycosylation sites that are found in the mouse protein. (davidson.edu)

The sequences for capture and intermediate oligonucleotides were designed and prepared as described previously. (cdc.gov)
These advances will allow mtDNA analysis in cases not previously examined by current sequencing-based approaches and could allow more forensic case samples to be entered into the proposed mtDNA Combined DNA Index System (CODIS trade mark ) databank as a result of mtDNA mixture resolution. (lookformedical.com)

Increasing use of fine needle aspiration for oncological diagnosis, while minimally invasive, poses a challenge for molecular testing by traditional sequencing platforms due to high sample requirements. (nih.gov)
Next-generation sequencing can be performed with only nanograms of DNA and has better sensitivity than traditional sequencing platforms. (nih.gov)

Common activities in bioinformatics include mapping and analyzing DNA and protein sequences, aligning different DNA and protein sequences to compare them, and creating and viewing 3-D models of protein structures. (limsforum.com)
Arguably one of the first "bioinformatics" projects-though the concept didn't yet exist-involved the 1965 creation and maintenance of a protein sequence database called the Atlas of Protein Sequence and Structure by Margaret O. Dayhoff, Richard V. Eck, and Robert S. Ledley. (limsforum.com)

We describe successful next-generation sequencing-based testing of fine needle aspiration cytological specimens in a clinical laboratory setting. (nih.gov)
of these, 31 specimens yielded sufficient DNA for next-generation sequencing testing. (nih.gov)

Essentially the most significant applications of expression array profiling to common disease are in the region of cancer. (biopaqc.com)

In preferred aspects the methods reduce the requirements for analysis of redundant sequences relative to that of methods that employ random sampling methods. (justia.com)

DNA sequencing and PCR analysis can also be performed to achieve accurate microbial identification and typing, but like current DNA typing procedures, these microbial DNA diagnostic tests require gel electrophoretic analysis, which is time consuming and labor intensive and accommodates a relatively low sample throughput. (justia.com)

The present invention relates generally to the fields of molecular biology and nucleic acid analysis. (justia.com)

Immobilization of modified and unmodified oligonucleotides on epoxy-functionalized glass surfaces is often used in microarray fabrication. (researchgate.net)

In the absence of quantitative predictive capacity for such bioaffinity interactions, high throughput experimental approaches are needed to sufficiently sample RNA sequence space. (nature.com)

Exome sequencing uncovered a relatively rare variant in MAPK3K11 on chromosome 11 (MAF = 0.01) that helped account for the suggestive linkage peak observed for the first principal component. (ox.ac.uk)
Each clone was hybridized to arrays of human chromosomes and gave signals on chromosome 11 in 20 cells. (davidson.edu)

The remaining amplified products were subjected to direct sequence analysis to obtain complete sequence information for the targeted HV regions. (lookformedical.com)
The DNA sequencing approach is therefore random and currently does not specifically sample any targeted regions of interest. (justia.com)

Elevated integrin α6 expression is involved in the occurrence and development of lung adenocarcinoma, and predicts a poor prognosis: a study based on immunohistochemical analysis and bioinformatics. (cancerindex.org)

Anatomy7

Organisms7

Diseases1

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes93

Disciplines and Occupations3

Technology, Industry, Agriculture2

Information Science9

Health Care7

Polymorphism7

Microarrays13

Genome17

Probes5

Proteins3

Genetics1

SNPs5

Primers6

Genes7

Short oligonucleotides1

Gene11

Genomic3

Illumina1

Polymerase6

High-density1

Synthesis5

Assay1

Detection3

Affymetrix1

Genomics3

Heteroduplex Analysis1

Amplification7

Deletions2

Mutation2

Molecules1

Conventional1

Methylation1

Tumor3

Biochemical2

Metastasis1

Mitochondrial2

Locus2

Probe3

Nucleic acids1

Variants3

Single3

Amino3

Previously2

Platforms2

Protein2

Specimens2

Disease1

Redundant1

Identification1

Molecular biology1

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Chromosome 112

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Adenocarcinoma1