• genome
  • MeV has six genes on its genome [nucleocapsid (N), phospho (P), matrix (M), fusion (F), hemagglutinin (H), and large (L)] and produces eight proteins (V and C proteins are produced from the P gene). (frontiersin.org)
  • Once sufficient P, N, L, and M2 proteins are available to create a capsid around the newly replicated genome, the virus undergoes replication. (wikipedia.org)
  • It has a single stranded positive RNA genome of about 9.3 kb and contains a single open reading frame (ORF) encoding two structural (E1 and E2) and five non-structural (NS2, NS3, NS4, NS5A, and NS5B) proteins. (wikipedia.org)
  • The nonstructural proteins NSm participate in virus assembly and NSs plays a key role in counteracting the host immune response by blocking alpha/beta interferon induction The full-length genome of NM/12 consists of a 947 base pair nucleotide S segment, a 4405 base pair nucleotide M segment, and a 6870 base pair nucelotide L segment. (wikipedia.org)
  • The orthobunyavirus genome has a characteristic segmented genome, with small, medium, and large (S, M, and L) segments which generally encode the nucleocapsid, envelope protein and the polymerase protein, respectively. (wikipedia.org)
  • Once the encapsidation region and the Gag protein interact, the NC domain recruits the genome of the virus to be packaged into the retrovirus capsid. (wikipedia.org)
  • In reassortment, the S and L segments are the ones that are usually exchanged between species further, the S segment, that is coded by the nucleocapsid protein, and the L polymerase function together to create a replication of the viral genome. (wikipedia.org)
  • Two genome subgroups can be distinguished, nonsegmented and segmented, and are described as such: In viruses with nonsegmented genomes, the first step of replication is transcription of the negative strand by RdRp to form various monocistronic mRNA that code for individual viral proteins. (wikipedia.org)
  • Gene
  • The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus(TGEV):comparison with the sequence of the peplomer protein of feline infectious peritonitis virus(FIPV). (nii.ac.jp)
  • Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. (nii.ac.jp)
  • To express and purify the nucleocapsid protein (N protein) of SARS-CoV and generate MAbs against the N protein, gene encoding N protein was separated into two parts according to the prediction of epitopes and cloned into pET32a(+), respectively. (mysciencework.com)
  • Thus, a general strategy for expression of any gene would be to generate significant quantities of mRNA containing the viral UTR from a strong promoter, and then to allow translation of mRNA of a gene product in the presence of N. Even a modest increase in the production of commercially desirable proteins is a goal in industry. (patentsencyclopedia.com)
  • and AUG=beginning (5' end) of the gene to be translated into protein. (patentsencyclopedia.com)
  • 8. The method according to claim 1 wherein said gene product is a protein or polypeptide. (patentsencyclopedia.com)
  • The Pol gene produces three proteins: a protease (PR), a reverse-transcriptase endowed with an RT (reverse-transcriptase) and an RNAse H domains, and an integrase (IN). (wikipedia.org)
  • Based on structure, presence/absence of specific protein domains, and conserved protein sequence motifs, they can be subdivided into several lineages: Errantiviruses contain an additional defective envelope ORF with similarities to the retroviral envelope gene. (wikipedia.org)
  • Virus
  • Computer model showing the hexameric structure of the toscana virus nucleocapsid protein. (sciencephoto.com)
  • Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus-type 1) complexed with the Psi RNA (ribonucleic acid) packaging element (pink). (sciencephoto.com)
  • Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid (N) protein may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. (tamu.edu)
  • 3. The method according to claim 1 wherein said nucleocapsid is from a virus from the genus hantavirus, orthobunyavirus, nairovirus, tospovirus or phlebovirus. (patentsencyclopedia.com)
  • The Pneumoviridae M2-1 protein is distinctive, and no homologue has been found in any other virus families. (wikipedia.org)
  • Identification of a Movement Protein of the Tenuvirus Rice Stripe Virus" (PDF). (wikipedia.org)
  • Type 2 PRRSV is an enveloped virus with a non-isometric nucleocapsid core. (wikipedia.org)
  • Extensive research has yet to be performed on the detailed crystalline structure of Batai virus, but research on the closely related Bunyamwera virus has shown a distinct functionality of each of the two nucleocapsid side chains. (wikipedia.org)
  • Gag proteins form virus-like particles in the cytoplasm inside which reverse-transcription occurs. (wikipedia.org)
  • Occasional ribosomal frameshifting allows the production of both proteins, while ensuring that much more Gag protein is produced to form virus-like particles. (wikipedia.org)
  • The virus might enter a cell using the cell surface protein nucleolin. (wikipedia.org)
  • The TTV1 virion contains four virus-encoded proteins, TP1-4. (wikipedia.org)
  • Interestingly, nucleocapsid protein TP1 has apparently evolved from a Cas4 endonuclease, a conserved component of the adaptive CRISPR-Cas immunity, presenting the first described case of exaptation of an enzyme for a virus capsid protein function. (wikipedia.org)
  • Matrix proteins inside the envelope stabilise virus structure. (wikipedia.org)
  • helical
  • 1995). The ability of E. coli expressed IBV N protein to form helical-nucleocapsid-like structures was investigated using transmission electron microscopy. (tamu.edu)
  • Because E. coli -expressed histidine-tagged fragments of the IBV N protein did not form helical nucleocapsid-like structures, the full-length protein is probably required for assembly of these structures. (tamu.edu)
  • Nucleocapsids have a diameter of 13.5 nm and a helical pitch of 6.5 nm. (wikipedia.org)
  • Nucleoprotein
  • In eukaryotic cells, DNA is associated with about an equal mass of histone proteins in a highly condensed nucleoprotein complex called chromatin. (wikipedia.org)
  • molecule
  • Ribosomes consist of one molecule of each of 50 or more ribosomal proteins along with three different molecules of RNA in prokaryotes or four in eukaryotes. (wikipedia.org)
  • Some viruses are simple ribonucleoproteins, containing only one molecule of RNA and a number of identical protein molecules. (wikipedia.org)
  • replication
  • If the encapsidation (packaging) signal is not essential for this particle assembly, then the protein interactions among Gag molecules are likely solely responsible for viral replication. (wikipedia.org)
  • coronavirus
  • Serum samples from 317 patients with patients with severe acute respiratory syndrome (SARS) were tested for the nucleocapsid (N) protein of SARS-associated coronavirus, with sensitivities of 94% and 78% for the first 5 days and 6-10 days after onset, respectively. (cdc.gov)
  • Molecular
  • The Northwestern blot, also known as the Northwestern assay, is a hybrid analytical technique of the Western blot and the Northern blot, and is used in molecular biology to detect interactions between RNA and proteins. (wikipedia.org)
  • Advantages of Northwestern blotting include the expedited detection of specific proteins that bind RNA, as well as the assessment of the approximate molecular weights of those proteins. (wikipedia.org)
  • complexes
  • Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand complexes in two dimensions, ACS Med. (rcsb.org)
  • infection
  • Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. (abcam.com)
  • Using pulse-labeling with 32Porthophosphate, the IBV N protein was found to be phosphorylated in the virion, as well as at all times during infection of Vero cells. (tamu.edu)
  • Detection
  • These techniques include the Western blot (protein detection), the Northern blot (RNA detection), the Southwestern blot (DNA-protein interaction detection), the Eastern blot (post translational modification detection) and the Northwestern blot (RNA-protein interaction detection). (wikipedia.org)
  • The Northwestern blot allows for detection of identified proteins in a way that is inexpensive. (wikipedia.org)
  • sequence
  • In addition, once the protein is isolated, it can be digested with trypsin, and Mass Spectrometry can be utilized to sequence the peptides in order to determine the identity of the specific protein. (wikipedia.org)