• genome
  • Retrotransposons are mobile genetic elements, closely related to retroviruses, which use their own proteins in conjunction with cellular machinery to replicate independently of the genome. (pubmedcentralcanada.ca)
  • Characterizing the Interactions between the Nucleocapsid Protein and the 5'-UTR of HIV-1: Implications for Diploid Genome Packaging. (umbc.edu)
  • Packaging is mediated by interactions between the nucleocapsid (NC) domain of the viral Gag polyproteins and the packaging segments located in the highly conserved 5'-untranslated region (5'-UTR) of the RNA genome . (umbc.edu)
  • Post a Comment for Characterizing the Interactions between the Nucleocapsid Protein and the 5'-UTR of HIV-1: Implications for Diploid Genome Packaging. (umbc.edu)
  • Retroviral genome recognition is mediated by the nucleocapsid (NC) domain of the virally encoded Gag polyprotein , which interacts with cognate RNA packaging elements that typically reside within the 5'-untranslated region (5'-UTR) of the genome . (umbc.edu)
  • Once sufficient P, N, L, and M2 proteins are available to create a capsid around the newly replicated genome, the virus undergoes replication. (wikipedia.org)
  • It has a single stranded positive RNA genome of about 9.3 kb and contains a single open reading frame (ORF) encoding two structural (E1 and E2) and five non-structural (NS2, NS3, NS4, NS5A, and NS5B) proteins. (wikipedia.org)
  • Once the encapsidation region and the Gag protein interact, the NC domain recruits the genome of the virus to be packaged into the retrovirus capsid. (wikipedia.org)
  • The nonstructural proteins NSm participate in virus assembly and NSs plays a key role in counteracting the host immune response by blocking alpha/beta interferon induction The full-length genome of NM/12 consists of a 947 base pair nucleotide S segment, a 4405 base pair nucleotide M segment, and a 6870 base pair nucelotide L segment. (wikipedia.org)
  • Two genome subgroups can be distinguished, nonsegmented and segmented, and are described as such: In viruses with nonsegmented genomes, the first step of replication is transcription of the negative strand by RdRp to form various monocistronic mRNA that code for individual viral proteins. (wikipedia.org)
  • In reassortment, the S and L segments are the ones that are usually exchanged between species further, the S segment, that is coded by the nucleocapsid protein, and the L polymerase function together to create a replication of the viral genome. (wikipedia.org)
  • Gene
  • The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus(TGEV):comparison with the sequence of the peplomer protein of feline infectious peritonitis virus(FIPV). (nii.ac.jp)
  • Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. (nii.ac.jp)
  • Thus, a general strategy for expression of any gene would be to generate significant quantities of mRNA containing the viral UTR from a strong promoter, and then to allow translation of mRNA of a gene product in the presence of N. Even a modest increase in the production of commercially desirable proteins is a goal in industry. (patentsencyclopedia.com)
  • and AUG=beginning (5' end) of the gene to be translated into protein. (patentsencyclopedia.com)
  • 8. The method according to claim 1 wherein said gene product is a protein or polypeptide. (patentsencyclopedia.com)
  • The Pol gene produces three proteins: a protease (PR), a reverse-transcriptase endowed with an RT (reverse-transcriptase) and an RNAse H domains, and an integrase (IN). (wikipedia.org)
  • Based on structure, presence/absence of specific protein domains, and conserved protein sequence motifs, they can be subdivided into several lineages: Errantiviruses contain an additional defective envelope ORF with similarities to the retroviral envelope gene. (wikipedia.org)
  • capsid
  • Interestingly, nucleocapsid protein TP1 has apparently evolved from a Cas4 endonuclease, a conserved component of the adaptive CRISPR-Cas immunity, presenting the first described case of exaptation of an enzyme for a virus capsid protein function. (wikipedia.org)
  • molecule
  • We use in vitro ensemble and single-molecule DNA stretching experiments to measure the characteristics of wild-type and mutant HIV-1 NC proteins, and correlate these results with cell-based HIV-1 replication assays. (blogspot.com)
  • Ribosomes consist of one molecule of each of 50 or more ribosomal proteins along with three different molecules of RNA in prokaryotes or four in eukaryotes. (wikipedia.org)
  • Some viruses are simple ribonucleoproteins, containing only one molecule of RNA and a number of identical protein molecules. (wikipedia.org)
  • Molecular
  • Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus-type 1) complexed with the Psi RNA (ribonucleic acid) packaging element (pink). (sciencephoto.com)
  • Advantages of Northwestern blotting include the expedited detection of specific proteins that bind RNA, as well as the assessment of the approximate molecular weights of those proteins. (wikipedia.org)
  • genomic
  • The prototypical examples are nucleosomes, complexes in which genomic DNA is wrapped around clusters of eight histone proteins in eukaryotic cell nuclei to form chromatin. (wikipedia.org)
  • In addition, negative-sense single-stranded RNA viruses have complex genomic sequences, cell cycles, and replication habits that use various protein complexes to arrange in specific conformations and carry out necessary processes for survival and reproduction of their genomic sequences. (wikipedia.org)
  • The genomic RNA segments are encapsidated by copies of the N protein in the form of ribonucleoprotein (RNP) complexes. (wikipedia.org)
  • mRNA
  • The mechanism of this augmentation relies on the ability of N to replace the cellular cap binding complex to attain more efficient translation initiation--the result being great mRNA production and greater protein/polypeptide production. (patentsencyclopedia.com)
  • Prior to undergoing replication, mRNA is transcribed and viral proteins are translated. (wikipedia.org)
  • A replicative complex is formed with RdRp: The positive strands can either function as mRNA to produce more proteins or as template to make more negative RNA strands. (wikipedia.org)
  • coronavirus
  • Serum samples from 317 patients with patients with severe acute respiratory syndrome (SARS) were tested for the nucleocapsid (N) protein of SARS-associated coronavirus, with sensitivities of 94% and 78% for the first 5 days and 6-10 days after onset, respectively. (cdc.gov)
  • zinc
  • The human immunodeficiency virus type 1 (HIV-1) nucleocapsid (NC) protein contains 15 basic residues located throughout its 55-amino acid sequence, as well as one aromatic residue in each of its two CCHC-type zinc finger motifs. (blogspot.com)
  • In the same manner as ADA, the compounds interact with an 18-residue polypeptide on the N terminal zinc knuckle region of the HIV nucleocapsid protein which causes ejection of the zinc from the region by covalently modifying the cysteine residues. (wikipedia.org)
  • binds
  • Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. (abcam.com)
  • During this time, the competitor RNA binds to the RNA binding proteins in the samples that are on the blot. (wikipedia.org)
  • Detection
  • These techniques include the Western blot (protein detection), the Northern blot (RNA detection), the Southwestern blot (DNA-protein interaction detection), the Eastern blot (post translational modification detection) and the Northwestern blot (RNA-protein interaction detection). (wikipedia.org)
  • The Northwestern blot allows for detection of identified proteins in a way that is inexpensive. (wikipedia.org)
  • sequence
  • In addition, once the protein is isolated, it can be digested with trypsin, and Mass Spectrometry can be utilized to sequence the peptides in order to determine the identity of the specific protein. (wikipedia.org)
  • structure
  • Extensive research has yet to be performed on the detailed crystalline structure of Batai virus, but research on the closely related Bunyamwera virus has shown a distinct functionality of each of the two nucleocapsid side chains. (wikipedia.org)
  • NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the psi-RNA packaging signal. (wikipedia.org)
  • involves
  • The technique involves using gel electrophoresis, an important analytical method that involves the use of an electric field and the subsequent migration of charged DNA, RNA or proteins through that electric field based on size and charge. (wikipedia.org)
  • Running a Northwestern blot involves separating the RNA binding proteins by gel electrophoresis, which will separate the RNA binding proteins based upon their size and charge. (wikipedia.org)
  • Results
  • After developing the blot using xray or autoradiography, the results can be analyzed and interpreted to determine the approximate size and concentration of the RNA binding protein(s) of interest to further study the protein(s). (wikipedia.org)
  • The location and concentration of the RNA binding protein on the blot can affect the results, and bands can sometimes appear after development. (wikipedia.org)